Fret Förster Resonance Energy Transfer

Author : Igor L. Medintz,Niko Hildebrandt
Publisher : John Wiley & Sons
Page : 824 pages
File Size : 42,8 Mb
Release : 2013-10-17
Category : Science
ISBN : 9783527656042

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FRET - Förster Resonance Energy Transfer by Igor L. Medintz,Niko Hildebrandt Pdf

FRET – Förster Resonance Energy Transfer Meeting the need for an up-to-date and detailed primer on all aspects of the topic, this ready reference reflects the incredible expansion in the application of FRET and its derivative techniques over the past decade, especially in the biological sciences. This wide diversity is equally mirrored in the range of expert contributors. The book itself is clearly subdivided into four major sections. The first provides some background, theory, and key concepts, while the second section focuses on some common FRET techniques and applications, such as in vitro sensing and diagnostics, the determination of protein, peptide and other biological structures, as well as cellular biosensing with genetically encoded fluorescent indicators. The third section looks at recent developments, beginning with the use of fluorescent proteins, followed by a review of FRET usage with semiconductor quantum dots, along with an overview of multistep FRET. The text concludes with a detailed and greatly updated series of supporting tables on FRET pairs and Förster distances, together with some outlook and perspectives on FRET. Written for both the FRET novice and for the seasoned user, this is a must-have resource for office and laboratory shelves.

Author : Theodorus W. J. Gadella
Publisher : Elsevier
Page : 560 pages
File Size : 49,5 Mb
Release : 2011-07-29
Category : Science
ISBN : 0080915124

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FRET and FLIM Techniques by Theodorus W. J. Gadella Pdf

This volume reviews the techniques Förster Resonance Energy Transfer (FRET) and Fluorescence Lifetime Imaging Microscopy (FLIM) providing researchers with step by step protocols and handy hints and tips. Both have become staple techniques in many biological and biophysical fields.

Author : Hilmi Volkan Demir,Sergey V. Gaponenko
Publisher : Cambridge University Press
Page : 453 pages
File Size : 49,6 Mb
Release : 2018-11-22
Category : Science
ISBN : 9781107145504

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Applied Nanophotonics by Hilmi Volkan Demir,Sergey V. Gaponenko Pdf

An accessible yet rigorous introduction to nanophotonics, covering basic principles, technology, and applications in lighting, lasers, and photovoltaics. Providing a wealth of information on materials and devices, and over 150 color figures, it is the 'go-to' guide for students in electrical engineering taking courses in nanophotonics.

Author : Igor L. Medintz,Niko Hildebrandt
Publisher : Wiley-VCH
Page : 0 pages
File Size : 50,9 Mb
Release : 2013-10-17
Category : Science
ISBN : 3527656030

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FRET - F¿rster Resonance Energy Transfer by Igor L. Medintz,Niko Hildebrandt Pdf

FRET – Förster Resonance Energy Transfer Meeting the need for an up-to-date and detailed primer on all aspects of the topic, this ready reference reflects the incredible expansion in the application of FRET and its derivative techniques over the past decade, especially in the biological sciences. This wide diversity is equally mirrored in the range of expert contributors. The book itself is clearly subdivided into four major sections. The first provides some background, theory, and key concepts, while the second section focuses on some common FRET techniques and applications, such as in vitro sensing and diagnostics, the determination of protein, peptide and other biological structures, as well as cellular biosensing with genetically encoded fluorescent indicators. The third section looks at recent developments, beginning with the use of fluorescent proteins, followed by a review of FRET usage with semiconductor quantum dots, along with an overview of multistep FRET. The text concludes with a detailed and greatly updated series of supporting tables on FRET pairs and Förster distances, together with some outlook and perspectives on FRET. Written for both the FRET novice and for the seasoned user, this is a must-have resource for office and laboratory shelves.

Author : Jeremy Sanderson
Publisher : John Wiley & Sons
Page : 848 pages
File Size : 54,6 Mb
Release : 2019-03-28
Category : Science
ISBN : 9781118696811

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Understanding Light Microscopy by Jeremy Sanderson Pdf

Introduces readers to the enlightening world of the modern light microscope There have been rapid advances in science and technology over the last decade, and the light microscope, together with the information that it gives about the image, has changed too. Yet the fundamental principles of setting up and using a microscope rests upon unchanging physical principles that have been understood for years. This informative, practical, full-colour guide fills the gap between specialised edited texts on detailed research topics, and introductory books, which concentrate on an optical approach to the light microscope. It also provides comprehensive coverage of confocal microscopy, which has revolutionised light microscopy over the last few decades. Written to help the reader understand, set up, and use the often very expensive and complex modern research light microscope properly, Understanding Light Microscopy keeps mathematical formulae to a minimum—containing and explaining them within boxes in the text. Chapters provide in-depth coverage of basic microscope optics and design; ergonomics; illumination; diffraction and image formation; reflected-light, polarised-light, and fluorescence microscopy; deconvolution; TIRF microscopy; FRAP & FRET; super-resolution techniques; biological and materials specimen preparation; and more. Gives a didactic introduction to the light microscope Encourages readers to use advanced fluorescence and confocal microscopes within a research institute or core microscopy facility Features full-colour illustrations and workable practical protocols Understanding Light Microscopy is intended for any scientist who wishes to understand and use a modern light microscope. It is also ideal as supporting material for a formal taught course, or for individual students to learn the key aspects of light microscopy through their own study.

Author : Igor L. Medintz,Niko Hildebrandt
Publisher : Wiley-VCH
Page : 0 pages
File Size : 41,5 Mb
Release : 2013-12-23
Category : Science
ISBN : 3527328165

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FRET - F¿rster Resonance Energy Transfer by Igor L. Medintz,Niko Hildebrandt Pdf

FRET – Förster Resonance Energy Transfer Meeting the need for an up-to-date and detailed primer on all aspects of the topic, this ready reference reflects the incredible expansion in the application of FRET and its derivative techniques over the past decade, especially in the biological sciences. This wide diversity is equally mirrored in the range of expert contributors. The book itself is clearly subdivided into four major sections. The first provides some background, theory, and key concepts, while the second section focuses on some common FRET techniques and applications, such as in vitro sensing and diagnostics, the determination of protein, peptide and other biological structures, as well as cellular biosensing with genetically encoded fluorescent indicators. The third section looks at recent developments, beginning with the use of fluorescent proteins, followed by a review of FRET usage with semiconductor quantum dots, along with an overview of multistep FRET. The text concludes with a detailed and greatly updated series of supporting tables on FRET pairs and Förster distances, together with some outlook and perspectives on FRET. Written for both the FRET novice and for the seasoned user, this is a must-have resource for office and laboratory shelves.

Author : Joseph R. Lakowicz
Publisher : Springer Science & Business Media
Page : 487 pages
File Size : 40,8 Mb
Release : 2013-11-11
Category : Science
ISBN : 9781461576587

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Principles of Fluorescence Spectroscopy by Joseph R. Lakowicz Pdf

Fluorescence methods are being used increasingly in biochemical, medical, and chemical research. This is because of the inherent sensitivity of this technique. and the favorable time scale of the phenomenon of fluorescence. 8 Fluorescence emission occurs about 10- sec (10 nsec) after light absorp tion. During this period of time a wide range of molecular processes can occur, and these can effect the spectral characteristics of the fluorescent compound. This combination of sensitivity and a favorable time scale allows fluorescence methods to be generally useful for studies of proteins and membranes and their interactions with other macromolecules. This book describes the fundamental aspects of fluorescence. and the biochemical applications of this methodology. Each chapter starts with the -theoreticalbasis of each phenomenon of fluorescence, followed by examples which illustrate the use of the phenomenon in the study of biochemical problems. The book contains numerous figures. It is felt that such graphical presentations contribute to pleasurable reading and increased understand ing. Separate chapters are devoted to fluorescence polarization, lifetimes, quenching, energy transfer, solvent effects, and excited state reactions. To enhance the usefulness of this work as a textbook, problems are included which illustrate the concepts described in each chapter. Furthermore, a separate chapter is devoted to the instrumentation used in fluorescence spectroscopy. This chapter will be especially valuable for those perform ing or contemplating fluorescence measurements. Such measurements are easily compromised by failure to consider a number of simple principles.

Author : Pedro Ludwig Hernández Martínez,Alexander Govorov,Hilmi Volkan Demir
Publisher : Unknown
Page : 42 pages
File Size : 44,8 Mb
Release : 2017
Category : Computer engineering
ISBN : 9811018723

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Understanding and Modeling Förster-type Resonance Energy Transfer (FRET) by Pedro Ludwig Hernández Martínez,Alexander Govorov,Hilmi Volkan Demir Pdf

Author : Hilmi Volkan Demir,Pedro Ludwig Hernández Martínez,Alexander Govorov
Publisher : Springer
Page : 40 pages
File Size : 54,6 Mb
Release : 2016-08-08
Category : Technology & Engineering
ISBN : 9789811018763

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Understanding and Modeling Förster-type Resonance Energy Transfer (FRET) by Hilmi Volkan Demir,Pedro Ludwig Hernández Martínez,Alexander Govorov Pdf

This Brief will focus on the functional uses and applications of FRET, starting with the derivation of FRET in the assemblies of nanostructures and subsequently giving application cases for biologists, physicists, chemists, material scientists, engineers, and those in many other fields whoever would like to FRET as a tool. The goal of this part is therefore to show both specialist and non-specialist how to use and analyze FRET in a wide range of applications.

Author : Martin Hof,Rudolf Hutterer,V. Fidler
Publisher : Springer Science & Business Media
Page : 330 pages
File Size : 47,9 Mb
Release : 2004-12-08
Category : Science
ISBN : 354022338X

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Fluorescence Spectroscopy in Biology by Martin Hof,Rudolf Hutterer,V. Fidler Pdf

Volume 3 of this new series focuses on brandnew research and applications in biology, biophysics and other fields of life sciences. Many frontline researcher have contributed to this highly attractive and interdisciplinary volume which spans the entire field of present fluorescence spectroscopy including nanotechnology, membrane and DNA studies and fluorescence imaging in cancer research.

Author : Chris D. Geddes,Joseph R. Lakowicz
Publisher : Springer Science & Business Media
Page : 621 pages
File Size : 40,7 Mb
Release : 2007-02-05
Category : Science
ISBN : 9780387330167

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Reviews in Fluorescence 2006 by Chris D. Geddes,Joseph R. Lakowicz Pdf

This is the third volume in the Reviews in Fluorescence series. To date, two volumes have been both published and well received by the scientific community. Several book reviews have also favorably described the series as an "excellent compilation of material which is well balanced from authors in both the US and Europe". Of particular mention we note the recent book review in JACS by Gary Baker, Los Alamos. In this 3rd volume we continue the tradition of publishing leading edge and timely articles from authors around the world. We hope you find this volume as useful as past volumes, which promises to be just as diverse with regard to content. Finally, in closing, we would like to thank Dr Kadir Asian for the typesetting of the entire volume and our counterparts at Springer, New York, for its timely publication. Professor Chris D. Geddes Professor Joseph R. Lakowicz August 20*^ 2005.

Author : Hilmi Volkan Demir,Pedro Ludwig Hernández Martínez,Alexander Govorov
Publisher : Unknown
Page : 40 pages
File Size : 47,7 Mb
Release : 2017
Category : Computer engineering
ISBN : 9811018758

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Understanding and Modeling Förster-type Resonance Energy Transfer (FRET) by Hilmi Volkan Demir,Pedro Ludwig Hernández Martínez,Alexander Govorov Pdf

Author : Yoshiaki Toyama,Atsushi Miyawaki,Masaya Nakamura,Masahiro Jinzaki
Publisher : Springer Nature
Page : 292 pages
File Size : 52,8 Mb
Release : 2019-10-02
Category : Medical
ISBN : 9789811379086

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Make Life Visible by Yoshiaki Toyama,Atsushi Miyawaki,Masaya Nakamura,Masahiro Jinzaki Pdf

This open access book describes marked advances in imaging technology that have enabled the visualization of phenomena in ways formerly believed to be completelyimpossible. These technologies have made major contributions to the elucidation of the pathology of diseases as well as to their diagnosis and therapy. The volume presents various studies from molecular imaging to clinical imaging. It also focuses on innovative, creative, advanced research that gives full play to imaging technology inthe broad sense, while exploring cross-disciplinary areas in which individual research fields interact and pursuing the development of new techniques where they fuse together. The book is separated into three parts, the first of which addresses the topic of visualizing and controlling molecules for life. Th e second part is devoted to imaging of disease mechanisms, while the final part comprises studies on the application of imaging technologies to diagnosis and therapy. Th e book contains the proceedings of the 12th Uehara International Symposium 2017, “Make Life Visible” sponsored by the Uehara Memorial Foundation and held from June 12 to 14, 2017. It is written by leading scientists in the field and is an open access publication under a CC BY 4.0 license.

Author : Dieter Bimberg
Publisher : Springer Science & Business Media
Page : 369 pages
File Size : 50,9 Mb
Release : 2008-06-03
Category : Technology & Engineering
ISBN : 9783540778998

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Semiconductor Nanostructures by Dieter Bimberg Pdf

Reducing the size of a coherently grown semiconductor cluster in all three directions of space to a value below the de Broglie wavelength of a charge carrier leads to complete quantization of the energy levels, density of states, etc. Such “quantum dots” are more similar to giant atoms in a dielectric cage than to classical solids or semiconductors showing a dispersion of energy as a function of wavevector. Their electronic and optical properties depend strongly on their size and shape, i.e. on their geometry. By designing the geometry by controlling the growth of QDs, absolutely novel possibilities for material design leading to novel devices are opened. This multiauthor book written by world-wide recognized leaders of their particular fields and edited by the recipient of the Max-Born Award and Medal 2006 Professor Dieter Bimberg reports on the state of the art of the growing of quantum dots, the theory of self-organised growth, the theory of electronic and excitonic states, optical properties and transport in a variety of materials. It covers the subject from the early work beginning of the 1990s up to 2006. The topics addressed in the book are the focus of research in all leading semiconductor and optoelectronic device laboratories of the world.

Author : Juliusz Ashot Kozak,James W. Putney, Jr.
Publisher : CRC Press
Page : 343 pages
File Size : 45,8 Mb
Release : 2017-07-14
Category : Science
ISBN : 9781498752732

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Calcium Entry Channels in Non-Excitable Cells by Juliusz Ashot Kozak,James W. Putney, Jr. Pdf

Calcium Entry Channels in Non-Excitable Cells focuses on methods of investigating the structure and function of non-voltage gated calcium channels. Each chapter presents important discoveries in calcium entry pathways, specifically dealing with the molecular identification of store-operated calcium channels which were reviewed by earlier volumes in the Methods in Signal Transduction series. Crystallographic and pharmacological approaches to the study of calcium channels of epithelial cells are also discussed. Calcium ion is a messenger in most cell types. Whereas voltage gated calcium channels have been studied extensively, the non-voltage gated calcium entry channel genes have only been identified relatively recently. The book will fill this important niche.