Gene Quantification

Author : Francois Ferre
Publisher : Springer Science & Business Media
Page : 379 pages
File Size : 46,6 Mb
Release : 2012-12-06
Category : Medical
ISBN : 9781461241645

Get Book

Gene Quantification by Francois Ferre Pdf

Geneticists and molecular biologists have been interested in quantifying genes and their products for many years and for various reasons (Bishop, 1974). Early molecular methods were based on molecular hybridization, and were devised shortly after Marmur and Doty (1961) first showed that denaturation of the double helix could be reversed - that the process of molecular reassociation was exquisitely sequence dependent. Gillespie and Spiegelman (1965) developed a way of using the method to titrate the number of copies of a probe within a target sequence in which the target sequence was fixed to a membrane support prior to hybridization with the probe - typically a RNA. Thus, this was a precursor to many of the methods still in use, and indeed under development, today. Early examples of the application of these methods included the measurement of the copy numbers in gene families such as the ribosomal genes and the immunoglo bulin family. Amplification of genes in tumors and in response to drug treatment was discovered by this method. In the same period, methods were invented for estimating gene num bers based on the kinetics of the reassociation process - the so-called Cot analysis. This method, which exploits the dependence of the rate of reassociation on the concentration of the two strands, revealed the presence of repeated sequences in the DNA of higher eukaryotes (Britten and Kohne, 1968). An adaptation to RNA, Rot analysis (Melli and Bishop, 1969), was used to measure the abundance of RNAs in a mixed population.

Author : Nalini Raghavachari,Natàlia Garcia-Reyero
Publisher : Humana
Page : 0 pages
File Size : 45,7 Mb
Release : 2018-05-17
Category : Medical
ISBN : 1493978330

Get Book

Gene Expression Analysis by Nalini Raghavachari,Natàlia Garcia-Reyero Pdf

This volume provides experimental and bioinformatics approaches related to different aspects of gene expression analysis. Divided in three sections chapters detail wet-lab protocols, bioinformatics approaches, single-cell gene expression, highly multiplexed amplicon sequencing, multi-omics techniques, and targeted sequencing. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Gene Expression Analysis: Methods and Protocols aims provide useful information to researchers worldwide.

Author : Bruce K. Patterson
Publisher : Springer Science & Business Media
Page : 157 pages
File Size : 45,5 Mb
Release : 2012-12-06
Category : Science
ISBN : 9781461213420

Get Book

Techniques in Quantification and Localization of Gene Expression by Bruce K. Patterson Pdf

Can the son or daughter of a baseball pitcher or cricket bowler throw a ball 100 miles an hour? Is the son or daughter of an opera singer also an opera singer? Is a house with functional light switches lit? The line of thinking in these rhetorical questions also applies to human genetics. What do baseball pitchers, opera sing ers, light switches, and the Human Genome Project have in common? These questions address the issue of potential versus realization of function. Although sons and daughters of baseball pitchers and opera singers may have inherited the mechanical attributes to be baseball pitchers and opera singers, they may not, at any point in time, be baseball pitchers or opera singers. A house with functional light switches is not lit unless the light switches are on. Similarly, all of the genes discovered and sequenced as a result of the Human Genome Project are not expressed at the same time. Genome project information will allow us to deter mine the repertoire of genes in an individual, which is analogous to determining where the light switches in a house are located and whether they are functional (a mutation or deletion in the Genome Project Model). The pattern of "on" light switches in a house gives us functional information as to what the family inside is doing (e. g. , eating, reading, sleeping). Similarly, the pattern of gene expression (RNA) gives us information on what our bodies are doing (e. g.

Author : Abraham Lee,Carolyn Ren
Publisher : Royal Society of Chemistry
Page : 315 pages
File Size : 41,6 Mb
Release : 2020-11-27
Category : Science
ISBN : 9781788017695

Get Book

Droplet Microfluidics by Abraham Lee,Carolyn Ren Pdf

Edited by two leaders, this book has drawn together expertise from around the globe to form a unified, cohesive resource for the droplet microfluidics community. Starting with the basic theory of droplet microfluidics before introducing its use as a tool, the reader is treated to chapters on important techniques, including robust passive and active droplet manipulations and applications such as single cell analysis, which is key for drug discovery. This book is a go-to resource for the community yearning to adopt and promote droplet microfluidics into different applications.

Author : Bruce Alberts
Publisher : Unknown
Page : 0 pages
File Size : 44,5 Mb
Release : 2002
Category : Cytology
ISBN : 0815332181

Get Book

Molecular Biology of The Cell by Bruce Alberts Pdf

Author : Giovanni Parmigiani,Elizabeth S. Garett,Rafael A. Irizarry,Scott L. Zeger
Publisher : Springer Science & Business Media
Page : 456 pages
File Size : 43,8 Mb
Release : 2006-04-11
Category : Medical
ISBN : 9780387216799

Get Book

The Analysis of Gene Expression Data by Giovanni Parmigiani,Elizabeth S. Garett,Rafael A. Irizarry,Scott L. Zeger Pdf

This book presents practical approaches for the analysis of data from gene expression micro-arrays. It describes the conceptual and methodological underpinning for a statistical tool and its implementation in software. The book includes coverage of various packages that are part of the Bioconductor project and several related R tools. The materials presented cover a range of software tools designed for varied audiences.

Author : Anonim
Publisher : Elsevier
Page : 730 pages
File Size : 53,8 Mb
Release : 2011-09-21
Category : Science
ISBN : 9780080521787

Get Book

Yeast Gene Analysis by Anonim Pdf

Focusing on Saccharomyces cerevisiae, the second edition of Yeast Gene Analysis represents a major reworking of the original edition, with many completely new chapters and major revisions to all previous chapters. Originally published shortly after completion of the yeast genome sequence, the new edition covers many of the major genome-wide strategies that have been developed since then such as microarray analysis of transcription, synthetic gene array studies, protein microarrays and chemical genetic approaches. It represents a valuable resource for any research laboratory using budding yeast as their experimental system in which to identify new yeast gene functions. The chapters are written in a readable style with useful background information, technical tips and specific experimental protocols included as appropriate, enabling both the novice and the experienced yeast researcher to adopt new procedures with confidence. New chapters on: Strain construction; genome-wide two-hybrid approaches; use of microarrays for transcript analysis; real-time analysis of chromosome behaviour and FRET; synthetic gene array technology and protein arrays; chemical genomics and yeast prions; RNA gene analysis and mitochondrial gene function analysis; phylogenetic footprinting; discovering human gene function and predicting yeast gene function

Author : Altuna Akalin
Publisher : CRC Press
Page : 462 pages
File Size : 49,9 Mb
Release : 2020-12-16
Category : Mathematics
ISBN : 9781498781862

Get Book

Computational Genomics with R by Altuna Akalin Pdf

Computational Genomics with R provides a starting point for beginners in genomic data analysis and also guides more advanced practitioners to sophisticated data analysis techniques in genomics. The book covers topics from R programming, to machine learning and statistics, to the latest genomic data analysis techniques. The text provides accessible information and explanations, always with the genomics context in the background. This also contains practical and well-documented examples in R so readers can analyze their data by simply reusing the code presented. As the field of computational genomics is interdisciplinary, it requires different starting points for people with different backgrounds. For example, a biologist might skip sections on basic genome biology and start with R programming, whereas a computer scientist might want to start with genome biology. After reading: You will have the basics of R and be able to dive right into specialized uses of R for computational genomics such as using Bioconductor packages. You will be familiar with statistics, supervised and unsupervised learning techniques that are important in data modeling, and exploratory analysis of high-dimensional data. You will understand genomic intervals and operations on them that are used for tasks such as aligned read counting and genomic feature annotation. You will know the basics of processing and quality checking high-throughput sequencing data. You will be able to do sequence analysis, such as calculating GC content for parts of a genome or finding transcription factor binding sites. You will know about visualization techniques used in genomics, such as heatmaps, meta-gene plots, and genomic track visualization. You will be familiar with analysis of different high-throughput sequencing data sets, such as RNA-seq, ChIP-seq, and BS-seq. You will know basic techniques for integrating and interpreting multi-omics datasets. Altuna Akalin is a group leader and head of the Bioinformatics and Omics Data Science Platform at the Berlin Institute of Medical Systems Biology, Max Delbrück Center, Berlin. He has been developing computational methods for analyzing and integrating large-scale genomics data sets since 2002. He has published an extensive body of work in this area. The framework for this book grew out of the yearly computational genomics courses he has been organizing and teaching since 2015.

Author : Janusz A. Z. Jankowski,Julia M. Polak
Publisher : Cambridge University Press
Page : 484 pages
File Size : 54,7 Mb
Release : 1996-09-13
Category : Medical
ISBN : 0521478960

Get Book

Clinical Gene Analysis and Manipulation by Janusz A. Z. Jankowski,Julia M. Polak Pdf

This practical compendium provides clinical scientists with an essential guide to the basic techniques of molecular medicine. It serves as a laboratory manual and a source of reference. It is suitable for those wishing to perform basic semi-quantitative experiments such as Northern or Southern blots and also those wishing to undertake more specialised genetic manipulations such as gene cloning, expression and creation of DNA libraries. It will give clinical scientists a unique insight into the potential of these techniques. As stated by Sir David Weatherall: 'It should be of great value to both established research workers and young scientists coming into the field for the first time. It deserves every success.'

Author : Piero Carninci
Publisher : CRC Press
Page : 273 pages
File Size : 50,6 Mb
Release : 2019-05-08
Category : Science
ISBN : 9780429533655

Get Book

Cap-Analysis Gene Expression (CAGE) by Piero Carninci Pdf

This book is a guide for users of new technologies, as it includes accurately proven protocols, allowing readers to prepare their samples for experiments. Additionally, it is a guide for the bioinformatics tools that are available for the analysis of the obtained tags, including the design of the software, the sources and the Web. Finally, the book provides examples of the application of these technologies to identify promoters, annotate genomes, identify new RNAs and reconstruct models of transcriptional control. Although examples mainly concern mammalians, the discussion expands to other groups of eukaryotes, where these approaches are complementing genome sequencing.

Author : Mei-Ling Ting Lee
Publisher : Springer Science & Business Media
Page : 378 pages
File Size : 55,6 Mb
Release : 2004-04-30
Category : Mathematics
ISBN : 9780792370871

Get Book

Analysis of Microarray Gene Expression Data by Mei-Ling Ting Lee Pdf

After genomic sequencing, microarray technology has emerged as a widely used platform for genomic studies in the life sciences. Microarray technology provides a systematic way to survey DNA and RNA variation. With the abundance of data produced from microarray studies, however, the ultimate impact of the studies on biology will depend heavily on data mining and statistical analysis. The contribution of this book is to provide readers with an integrated presentation of various topics on analyzing microarray data.

Author : W. Dietmaier,C. Wittwer,N. Sivasubramanian
Publisher : Springer Science & Business Media
Page : 200 pages
File Size : 41,7 Mb
Release : 2013-06-29
Category : Medical
ISBN : 9783642593970

Get Book

Rapid Cycle Real-Time PCR — Methods and Applications by W. Dietmaier,C. Wittwer,N. Sivasubramanian Pdf

Rapid-Cycle Real-Time PCR is a powerful technique for nucleic acid amplification and analysis that often requires less than half an hour to perform. Samples are amplified by rapid-cycle PCR followed by immediate melting curve analysis in the same instrument. Melting curve analysis of PCR products with SYBR Green I often allows product identification without gel electrophoresis. Furthermore, in the presence of fluorescent hybridization probes, melting curves provide "dynamic dot blots" for fine sequence analysis, including single nucleotide polymorphisms (SNPs). The method is often cited as the most versatile, efficient method for nucleic acid analysis in research and diagnostics in the fields of genetics and oncology. Molecular diagnostics has never been easier!

Author : Philip Meneely
Publisher : Oxford University Press, USA
Page : 491 pages
File Size : 48,5 Mb
Release : 2020-02-11
Category : Eukaryotic cells
ISBN : 9780198809906

Get Book

Genetic Analysis by Philip Meneely Pdf

How do we know what role a particular gene has? How do some genes control the expression of others? How do genes interact to form gene networks? With its unique integration of genetics and molecular biology, Genetic Analysis probes fascinating questions such as these, detailing how our understanding of key genetic phenomena can be used to understand biological systems. Opening with a brief overview of key genetic principles, model organisms, and epigenetics, the book goes on to explore the use of gene mutations and the analysis of gene expression and activity. A discussion of the interactions of genes during suppression, synthetic enhancement, and epistasis follows, which is then expanded into a consideration of genetic networks and personal genomics. Drawing on the latest experimental tools, including CRISPR-Cas9 genome editing, microarrays, RNAi screens, and bioinformatics approaches, Genetic Analysis provides a state-of-the-art review of the field, but in a truly student-friendly manner. It uses extended case studies and text boxes to augment the narrative, taking the reader right to the forefront of contemporary research, without losing its clarity of explanation and insight. We are in an age where, despite knowing so much about biological systems, we are just beginning to realise how much more there is still to understand. Genetic Analysis is the ideal guide to how we can use the awesome power of molecular genetics to further our understanding.

Author : Robert C. Bast, Jr.,Carlo M. Croce,William N. Hait,Waun Ki Hong,Donald W. Kufe,Martine Piccart-Gebhart,Raphael E. Pollock,Ralph R. Weichselbaum,Hongyang Wang,James F. Holland
Publisher : John Wiley & Sons
Page : 2008 pages
File Size : 47,7 Mb
Release : 2017-03-10
Category : Medical
ISBN : 9781119000846

Get Book

Holland-Frei Cancer Medicine by Robert C. Bast, Jr.,Carlo M. Croce,William N. Hait,Waun Ki Hong,Donald W. Kufe,Martine Piccart-Gebhart,Raphael E. Pollock,Ralph R. Weichselbaum,Hongyang Wang,James F. Holland Pdf

Holland-Frei Cancer Medicine, Ninth Edition, offers a balanced view of the most current knowledge of cancer science and clinical oncology practice. This all-new edition is the consummate reference source for medical oncologists, radiation oncologists, internists, surgical oncologists, and others who treat cancer patients. A translational perspective throughout, integrating cancer biology with cancer management providing an in depth understanding of the disease An emphasis on multidisciplinary, research-driven patient care to improve outcomes and optimal use of all appropriate therapies Cutting-edge coverage of personalized cancer care, including molecular diagnostics and therapeutics Concise, readable, clinically relevant text with algorithms, guidelines and insight into the use of both conventional and novel drugs Includes free access to the Wiley Digital Edition providing search across the book, the full reference list with web links, illustrations and photographs, and post-publication updates

Author : T. A. Brown
Publisher : John Wiley & Sons
Page : 432 pages
File Size : 44,6 Mb
Release : 2020-11-23
Category : Science
ISBN : 9781119640783

Get Book

Gene Cloning and DNA Analysis by T. A. Brown Pdf

Known worldwide as the standard introductory text to this important and exciting area of study, Gene Cloning and DNA Analysis: An Introduction, 8th Edition preserves the tradition of excellence created by previous editions. Comprehensive and authoritative, the book explores all of the topics crucial to an understanding of gene cloning in an approachable way. An easy-to-follow and user-friendly layout is presented in full-color throughout the volume, making it simple to absorb the clear and accessible material contained within. Gene Cloning and DNA Analysis: An Introduction, 8th Edition contains updated and extended coverage of gene editing strategies like CRISPR/Cas, rewritten chapters on DNA sequencing and genome studies, as well as new material on real-time PCR and typing of human disease mutations. Over 250 full-color illustrations are included to bring to life the comprehensive content. The book also covers topics like: The strategies used by researchers and industry practitioners to assemble genome sequences Next generation sequencing methods and descriptions of their applications in studying genomes and transcriptomes Includes the use and application of gene editing strategies Interbreeding between Neanderthals and Homo Sapiens Gene Cloning and DNA Analysis: An Introduction, 8th Edition is an invaluable introductory text for students in classes like genetics and genomics, molecular biology, biochemistry, immunology, and applied biology. It also belongs on the bookshelves of every professional who desires to improve their understanding of the basics of gene cloning or DNA analysis.