Rapid Cycle Real Time Pcr Methods And Applications

Author : Carl Wittwer,Meinhard Hahn,Karen Kaul
Publisher : Springer
Page : 223 pages
File Size : 46,6 Mb
Release : 2012-12-06
Category : Science
ISBN : 9783642188404

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Rapid Cycle Real-Time PCR — Methods and Applications by Carl Wittwer,Meinhard Hahn,Karen Kaul Pdf

Rapid Cycle Real-Time PCR is a powerful technique for nucleic acid quantification and analysis that takes less than 30 minutes to complete. Fluorescence is automatically monitored each cycle and the amount of template quantified by advanced analytical methods, such as the second derivative maximum method. Immediately following rapid cycle PCR, melting curve analysis is performed to verify product purity with SYBR Green I and/or genotype with fluorescently-labeled hybridization probes(HybProbes or SimpleProbes). Rapid cycle real-time PCR is often cited as the most versatile, efficient method for nucleic acid quantification in research and climical studies. Molecular analysis has never been easier!

Author : W. Dietmaier,C. Wittwer,N. Sivasubramanian
Publisher : Springer Science & Business Media
Page : 200 pages
File Size : 44,7 Mb
Release : 2013-06-29
Category : Medical
ISBN : 9783642593970

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Rapid Cycle Real-Time PCR — Methods and Applications by W. Dietmaier,C. Wittwer,N. Sivasubramanian Pdf

Rapid-Cycle Real-Time PCR is a powerful technique for nucleic acid amplification and analysis that often requires less than half an hour to perform. Samples are amplified by rapid-cycle PCR followed by immediate melting curve analysis in the same instrument. Melting curve analysis of PCR products with SYBR Green I often allows product identification without gel electrophoresis. Furthermore, in the presence of fluorescent hybridization probes, melting curves provide "dynamic dot blots" for fine sequence analysis, including single nucleotide polymorphisms (SNPs). The method is often cited as the most versatile, efficient method for nucleic acid analysis in research and diagnostics in the fields of genetics and oncology. Molecular diagnostics has never been easier!

Author : W. Dietmaier,Carl Wittwer,N. Sivasubramanian
Publisher : Unknown
Page : 218 pages
File Size : 55,6 Mb
Release : 2014-01-15
Category : Electronic
ISBN : 3642593984

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Rapid Cycle Real-Time PCR - Methods and Applications by W. Dietmaier,Carl Wittwer,N. Sivasubramanian Pdf

Author : U. Reischl,C. Wittwer,F. Cockerill
Publisher : Springer Science & Business Media
Page : 253 pages
File Size : 42,7 Mb
Release : 2012-12-06
Category : Science
ISBN : 9783642483516

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Rapid Cycle Real-Time PCR — Methods and Applications by U. Reischl,C. Wittwer,F. Cockerill Pdf

Rapid Cycle Real-Time PCR is a powerful analytical tool with broad application for the basic and applied life sciences. Compared with conventional PCR technology, Rapid Cycle Real-Time PCR is faster, has greater specificity, and is more easily adaptable for a variety of diagnostic tests, including qualitative, quantitative and mutation detection assays. This book provides general overviews of this technology for use in the clinical microbiology laboratory as well as specific diagnostic protocols for the detection of viral, bacterial and fungal pathogens and genetically modified organisms in human specimens and foodstuffs. All of these protocols have been developed, verified, and validated by experts in the field and should be of great interest for clinical microbiologists, pathologists, laboratory technologists as well as practicing physicians.

Author : Udo Reischl,Carl Wittwer,F. Cockerill
Publisher : Springer
Page : 258 pages
File Size : 49,9 Mb
Release : 2012-12-12
Category : Science
ISBN : 3642483534

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Rapid Cycle Real-Time PCR — Methods and Applications by Udo Reischl,Carl Wittwer,F. Cockerill Pdf

Rapid Cycle Real-Time PCR is a powerful analytical tool with broad application for the basic and applied life sciences. Compared with conventional PCR technology, Rapid Cycle Real-Time PCR is faster, has greater specificity, and is more easily adaptable for a variety of diagnostic tests, including qualitative, quantitative and mutation detection assays. This book provides general overviews of this technology for use in the clinical microbiology laboratory as well as specific diagnostic protocols for the detection of viral, bacterial and fungal pathogens and genetically modified organisms in human specimens and foodstuffs. All of these protocols have been developed, verified, and validated by experts in the field and should be of great interest for clinical microbiologists, pathologists, laboratory technologists as well as practicing physicians.

Author : Francois Ferre
Publisher : Springer Science & Business Media
Page : 379 pages
File Size : 43,5 Mb
Release : 2012-12-06
Category : Medical
ISBN : 9781461241645

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Gene Quantification by Francois Ferre Pdf

Geneticists and molecular biologists have been interested in quantifying genes and their products for many years and for various reasons (Bishop, 1974). Early molecular methods were based on molecular hybridization, and were devised shortly after Marmur and Doty (1961) first showed that denaturation of the double helix could be reversed - that the process of molecular reassociation was exquisitely sequence dependent. Gillespie and Spiegelman (1965) developed a way of using the method to titrate the number of copies of a probe within a target sequence in which the target sequence was fixed to a membrane support prior to hybridization with the probe - typically a RNA. Thus, this was a precursor to many of the methods still in use, and indeed under development, today. Early examples of the application of these methods included the measurement of the copy numbers in gene families such as the ribosomal genes and the immunoglo bulin family. Amplification of genes in tumors and in response to drug treatment was discovered by this method. In the same period, methods were invented for estimating gene num bers based on the kinetics of the reassociation process - the so-called Cot analysis. This method, which exploits the dependence of the rate of reassociation on the concentration of the two strands, revealed the presence of repeated sequences in the DNA of higher eukaryotes (Britten and Kohne, 1968). An adaptation to RNA, Rot analysis (Melli and Bishop, 1969), was used to measure the abundance of RNAs in a mixed population.

Author : Erika Pestana,Sandor Belak,Adama Diallo,John R. Crowther,Gerrit J. Viljoen
Publisher : Springer Science & Business Media
Page : 320 pages
File Size : 44,5 Mb
Release : 2010-01-18
Category : Science
ISBN : 9789048131327

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Early, rapid and sensitive veterinary molecular diagnostics - real time PCR applications by Erika Pestana,Sandor Belak,Adama Diallo,John R. Crowther,Gerrit J. Viljoen Pdf

This book gives a comprehensive account of the practical aspects of Real time PCR and its application to veterinary diagnostic laboratories. The optimisation of assays to help diagnose livestock diseases is stressed and exemplified through assembling standard operating procedures from many laboratory sources. Theoretical aspects of PCR are dealt with as well as quality control features necessary to maintain an assured testing system. The book will be helpful to all scientists involved in diagnostic applications of molecular techniques, but is designed primarily to offer developing country scientists a collection of working methods in a single source. The book is an adjunct to the Molecular Diagnostic PCR Handbook published in 2005.

Author : Kirstin J. Edwards,Julie M. J. Logan,Nick A. Saunders
Publisher : Taylor & Francis
Page : 362 pages
File Size : 42,9 Mb
Release : 2004
Category : Polymerase chain reaction
ISBN : 9781134184002

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Real-Time PCR by Kirstin J. Edwards,Julie M. J. Logan,Nick A. Saunders Pdf

Author : M Tevfik Dorak
Publisher : Garland Science
Page : 333 pages
File Size : 41,7 Mb
Release : 2007-02-08
Category : Science
ISBN : 9780203967317

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Real-time PCR by M Tevfik Dorak Pdf

With a variety of detection chemistries, an increasing number of platforms, multiple choices for analytical methods and the jargon emerging along with these developments, real-time PCR is facing the risk of becoming an intimidating method, especially for beginners. Real-time PCR provides the basics, explains how they are exploited to run a real-time PCR assay, how the assays are run and where these assays are informative in real life. It addresses the most practical aspects of the techniques with the emphasis on 'how to do it in the laboratory'. Keeping with the spirit of the Advanced Methods Series, most chapters provide an experimental protocol as an example of a specific assay.

Author : Lucília Domingues
Publisher : Springer Nature
Page : 255 pages
File Size : 51,9 Mb
Release : 2023-09-23
Category : Science
ISBN : 9781071633588

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PCR by Lucília Domingues Pdf

This second volume focuses on PCR methods and PCR application specificities to the biotechnology and bioengineering field. New and updated chapters detail real-time PCR protocols, synthetic biology applications, pathogen detection, microfluidics, digital, multiplex detection recent advances. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, PCR: Methods and Protocols, Second Edition aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge.

Author : Roberto Biassoni,Alessandro Raso
Publisher : Humana
Page : 0 pages
File Size : 48,8 Mb
Release : 2019-10-03
Category : Science
ISBN : 1493998323

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Quantitative Real-Time PCR by Roberto Biassoni,Alessandro Raso Pdf

This book expands upon the useful first edition by exploring classic Quantitative Polymerase Chain Reaction (qPCR) techniques as well as a number of recently developed applications. With the changes in instrumentation due to technological advances and the development of new reagents to fulfill ethical and legal issues, the qPCR field is now an up-to-date technology that indeed is widely used in research and clinical diagnostics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Revised and authoritative, Quantitative Real-Time PCR: Methods and Protocols, Second Edition is an ideal guide to this expanding and vital field of study.

Author : Nicola King,Joe O’Connell
Publisher : Springer Science & Business Media
Page : 370 pages
File Size : 53,8 Mb
Release : 2008-02-04
Category : Science
ISBN : 9781592592838

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RT-PCR Protocols by Nicola King,Joe O’Connell Pdf

Until the mid 1980s, the detection and quantification of a specific mRNA was a difficult task, usually only undertaken by a skilled molecular biologist. With the advent of PCR, it became possible to amplify specific mRNA, after first converting the mRNA to cDNA via reverse transcriptase. The arrival of this technique—termed reverse transcription-PCR (RT-PCR)—meant that mRNA suddenly became amenable to rapid and sensitive analysis, without the need for advanced training in molecular biology. This new accessibility of mRNA, which has been facilitated by the rapid accumulation of sequence data for human mRNAs, means that every biomedical researcher can now include measurement of specific mRNA expression as a routine component of his/her research plans. In view of the ubiquity of the use of standard RT-PCR, the main objective of RT-PCR Protocols is essentially to provide novel, useful applications of RT-PCR. These include some useful adaptations and applications that could be relevant to the wider research community who are already familiar with the basic RT-PCR protocol. For example, a variety of different adaptations are described that have been employed to obtain quantitative data from RT-PCR. Quantitative RT-PCR provides the ability to accurately measure changes/imb- ances in specific mRNA expression between normal and diseased tissues.

Author : Stephen A. Bustin
Publisher : Cambridge University Press
Page : 327 pages
File Size : 50,6 Mb
Release : 2010
Category : Science
ISBN : 9780521882316

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The PCR Revolution by Stephen A. Bustin Pdf

Examines the latest innovations and the overall impact of PCR on areas of molecular research.

Author : Kary B. Mullis,Francois Ferre,Richard A. Gibbs
Publisher : Springer Science & Business Media
Page : 464 pages
File Size : 51,8 Mb
Release : 2012-02-02
Category : Medical
ISBN : 9781461202578

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The Polymerase Chain Reaction by Kary B. Mullis,Francois Ferre,Richard A. Gibbs Pdf

James D. Watson When, in late March of 1953, Francis Crick and I came to write the first Nature paper describing the double helical structure of the DNA molecule, Francis had wanted to include a lengthy discussion of the genetic implications of a molecule whose struc ture we had divined from a minimum of experimental data and on theoretical argu ments based on physical principles. But I felt that this might be tempting fate, given that we had not yet seen the detailed evidence from King's College. Nevertheless, we reached a compromise and decided to include a sentence that pointed to the biological significance of the molecule's key feature-the complementary pairing of the bases. "It has not escaped our notice," Francis wrote, "that the specific pairing that we have postulated immediately suggests a possible copying mechanism for the genetic material." By May, when we were writing the second Nature paper, I was more confident that the proposed structure was at the very least substantially correct, so that this second paper contains a discussion of molecular self-duplication using templates or molds. We pointed out that, as a consequence of base pairing, a DNA molecule has two chains that are complementary to each other. Each chain could then act ". . . as a template for the formation on itself of a new companion chain, so that eventually we shall have two pairs of chains, where we only had one before" and, moreover, " ...

Author : Roberto Biassoni,Alessandro Raso
Publisher : Humana
Page : 0 pages
File Size : 53,7 Mb
Release : 2014-04-17
Category : Science
ISBN : 1493907328

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Quantitative Real-Time PCR by Roberto Biassoni,Alessandro Raso Pdf

Quantitative Real-Time PCR: Methods and Protocols focuses on different applications of qPCR ranging from microbiological detections (both viral and bacterial) to pathological applications. Several chapters deal with quality issues which regard the quality of starting material, the knowledge of the minimal information required to both perform an assay and to set the experimental plan, while the others focus on translational medicine applications that are ordered following an approximate logical order of their medical application. The last part of the book gives you an idea of an emerging digital PCR technique that is a unique qPCR approach for measuring nucleic acid, particularly suited for low level detection and to develop non-invasive diagnosis. Written for the Methods in Molecular Biology series, most chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, laboratory protocols and tips on troubleshooting and avoiding known pitfalls. Practical and authoritative, Quantitative Real-Time PCR: Methods and Protocols aims to aid researchers seeking to devise new qPCR-based approaches related to his or her area of investigation.