Gene Therapy Protocols

Gene Therapy Protocols Book in PDF, ePub and Kindle version is available to download in english. Read online anytime anywhere directly from your device. Click on the download button below to get a free pdf file of Gene Therapy Protocols book. This book definitely worth reading, it is an incredibly well-written.

Gene Therapy Protocols

Paul D. Robbins
Gene Therapy Protocols Book PDF

Author : Paul D. Robbins
Publisher : Humana PressInc
Page : 432 pages
File Size : 49,6 Mb
Release : 1997
Category : Medical
ISBN : 0896034844

Get Book

Gene Therapy Protocols by Paul D. Robbins Pdf

Thirty protocols by leading researchers describe in detail all the essential molecular methods for working with gene transfer systems, along with the methods for gene transfer to specific tissue types either in vivo or ex vivo. The easily reproducible methods range from those for specific viral and nonviral for both genetic and acquired diseases, to those concerned with gene delivery to particular tissues. Methods for applying specific therapeutic systems, such as ribozymes and tumor supressor genes for the treatment of AIDS and cancer, are also included in this authoritative collection.

Gene Therapy Protocols

Joseph LeDoux
Gene Therapy Protocols Book PDF

Author : Joseph LeDoux
Publisher : Humana Press
Page : 408 pages
File Size : 41,7 Mb
Release : 2008-04-24
Category : Medical
ISBN : 1588299031

Get Book

Gene Therapy Protocols by Joseph LeDoux Pdf

In this book internationally recognized investigators describe cutting-edge laboratory techniques for the study of Production and In Vivo Applications of Gene Transfer Vectors and Design and Characterization of Gene Transfer Vectors. Readers will find a comprehensive resource of current and emerging methods for the production of viral and non-viral gene transfer vectors, as well as detailed protocols for applications in stem cell biology, cancer research and infectious disease.

Gene Therapy Protocols

Paul D. Robbins
Gene Therapy Protocols Book PDF

Author : Paul D. Robbins
Publisher : Unknown
Page : 432 pages
File Size : 41,9 Mb
Release : 1997
Category : Gene therapy
ISBN : 159259591X

Get Book

Gene Therapy Protocols by Paul D. Robbins Pdf

In Gene Therapy Protocols, leading researchers describe in detail all the essential molecular methods for developing gene transfer systems, along with the methods for introducing genes into specific tissue types either in vivo or ex vivo. These easily reproducible methods range from those for specific viral and nonviral delivery systems, to those concerned with gene delivery to particular tissues. Methods for applying specific therapeutic systems, such as ribozymes and tumor suppressor genes for the treatment of AIDS and cancer, are also included in this authoritative collection. Gene Therapy Protocols is the first major collection of the methods needed for successful gene delivery and subsequent in vivo gene expression, techniques at the center of the many and significant recent advances in the treatment of both genetic and acquired diseases. It will surely become today's indispensable standard reference source for all scientists working to realize the promises of gene therapy.

Viral Vectors for Gene Therapy

Curtis A. Machida
Viral Vectors for Gene Therapy Book PDF

Author : Curtis A. Machida
Publisher : Springer Science & Business Media
Page : 591 pages
File Size : 40,6 Mb
Release : 2008-02-02
Category : Medical
ISBN : 9781592593040

Get Book

Viral Vectors for Gene Therapy by Curtis A. Machida Pdf

Viral Vectors for Gene Therapy: Methods and Protocols consists of 30 ch- ters detailing the use of herpes viruses, adenoviruses, adeno-associated viruses, simple and complex retroviruses, including lentiviruses, and other virus systems for vector development and gene transfer. Chapter cont- butions provide perspective in the use of viral vectors for applications in the brain and in the central nervous system. Viral Vectors for Gene Therapy: Methods and Protocols contains step-by-step methods for successful rep- cation of experimental procedures, and should prove useful for both experienced investigators and newcomers in the field, including those beginning graduate study or undergoing postdoctoral training. The “Notes” section contained in each chapter provides valuable troublesho- ing guides to help develop working protocols for your laboratory. With Viral Vectors for Gene Therapy: Methods and Protocols, it has been my intent to develop a comprehensive collection of modern molecular methods for the construction, development, and use of viral vectors for gene transfer and gene therapy. I would like to thank the many chapter authors for their contributions. They are all experts in various aspects of viral vectors, and I appreciate their efforts and hard work in developing comprehensive chapters. As editor, it has been a privilege to preview the development of Viral Vectors for Gene Therapy: Methods and Protocols, and to acquire insight into the various methodological approaches from the many different contri- tors.

Viral Vectors for Gene Therapy

Fredric P. Manfredsson,Matthew J. Benskey
Viral Vectors for Gene Therapy Book PDF

Author : Fredric P. Manfredsson,Matthew J. Benskey
Publisher : Unknown
Page : 328 pages
File Size : 48,7 Mb
Release : 2019
Category : Electronic books
ISBN : 1493990659

Get Book

Viral Vectors for Gene Therapy by Fredric P. Manfredsson,Matthew J. Benskey Pdf

This volume discusses protocols, ranging from vector production to delivery methods, used to execute gene therapy applications. Chapters are divided into four parts, and cover topics such as design, construction, and application of transcription activation-like effectors; multi-modal production of adeno-associated virus; construction of oncolytic herpes simplex virus; AAV-mediated gene delivery to the mouse liver; and intrathecal delivery of gene therapeutics by direct lumbar puncture in mice. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and authoritative, Viral Vectors for Gene Therapy: Methods and Protocols is a valuable resource for researchers, clinicians, and students looking to utilize viral vectors in gene therapy experiments.

Gene Therapy Protocols

Joseph LeDoux
Gene Therapy Protocols Book PDF

Author : Joseph LeDoux
Publisher : Humana Press
Page : 0 pages
File Size : 53,7 Mb
Release : 2014-11-04
Category : Medical
ISBN : 1627039643

Get Book

Gene Therapy Protocols by Joseph LeDoux Pdf

In Gene Therapy Protocols, Volumes 1 and 2, internationally recognized investigators describe cutting-edge laboratory techniques for the study of Production and In Vivo Applications of Gene Transfer Vectors (Volume 1) and Design and Characterization of Gene Transfer Vectors (Volume 2). In this second volume, readers will find a comprehensive resource of current and emerging methods for the processing and characterization of viral and non-viral gene transfer vectors.

Gene Therapy Protocols

Joseph M. Le Doux
Gene Therapy Protocols Book PDF

Author : Joseph M. Le Doux
Publisher : Unknown
Page : 128 pages
File Size : 45,6 Mb
Release : 2008
Category : Electronic books
ISBN : 1603272488

Get Book

Gene Therapy Protocols by Joseph M. Le Doux Pdf

In the first volume, readers will find a comprehensive resource of current and emerging methods for the production of viral and non-viral gene transfer vectors, as well as detailed protocols for critical applications in stem cell biology, cancer, diabetes, HIV and tissue engineering. In the second volume, readers will find a comprehensive resource of current and emerging methods for the processing and characterization of viral and non-viral gene transfer vectors, as well as promising approaches to design vectors for efficient, targeted and regulated gene delivery and expression.

Viral Vectors for Gene Therapy

Otto-Wilhelm Merten,Mohamed Al-Rubeai
Viral Vectors for Gene Therapy Book PDF

Author : Otto-Wilhelm Merten,Mohamed Al-Rubeai
Publisher : Humana
Page : 0 pages
File Size : 40,5 Mb
Release : 2011-05-19
Category : Medical
ISBN : 161779094X

Get Book

Viral Vectors for Gene Therapy by Otto-Wilhelm Merten,Mohamed Al-Rubeai Pdf

The huge potential for gene therapy to cure a wide range of diseases has led to high expectations and a great increase in research efforts in this area, particularly in the study of delivery via viral vectors, widely considered to be more efficient than DNA transfection. In Viral Vectors for Gene Therapy: Methods and Protocols, experts in the field present a collection of their knowledge and experience featuring methodologies that involve virus production, transferring protocols, and evaluating the efficacy of gene products. While thoroughly covering the most popular viral vector systems of adenovirus, retrovirus, and adeno-associated virus, this detailed volume also explores less common viral vector systems such as baculovirus, herpes virus, and measles virus, the growing interest in which is creating a considerable demand for large scale manufacturing and purification procedures. Written in the highly successful Methods in Molecular BiologyTM series format, many chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and vital tips on troubleshooting and avoiding known pitfalls. Comprehensive and practical, Viral Vectors for Gene Therapy: Methods and Protocols provides basic principles accessible to scientists from a wide variety of backgrounds for the development of gene therapy viral products that are safe and effective.

Lentivirus Gene Engineering Protocols

Maurizio Federico
Lentivirus Gene Engineering Protocols Book PDF

Author : Maurizio Federico
Publisher : Springer Science & Business Media
Page : 310 pages
File Size : 47,5 Mb
Release : 2008-02-03
Category : Science
ISBN : 9781592593934

Get Book

Lentivirus Gene Engineering Protocols by Maurizio Federico Pdf

Cell gene engineering is emerging as a field with outstanding impact, not only in medicine/biology, but also, and perhaps most importantly, in agriculture and in all those food sciences involved in the fight against world hunger. Lentivirus vector-based technologies represent the last frontier in the development of powerful and reliable methods for both in vitro and in vivo gene transfer in eukaryotic animal cells. Although the design of lentivirus vectors is closely reminiscent of those already successfully applied to the construction of oncoretroviral vectors, some unique features, e.g., the ef- ciency in transducing both postmitotic and stem cells, render the use of lentivirus vectors invaluable. It has been a great pleasure to edit Lentivirus Gene Engineering Pro- cols, owing in part to the high level of enthusiasm that the authors dem- strated in contributing to this book. The fact that so many outstanding scientists engaged in lentivirus vector research have provided articles renders it so- thing more than a technical handbook. In addition to detailed descriptions of the most innovative methodologies, the reader may find very informative ov- views concerning both theoretical and practical aspects of the origin and the development of diverse lentivirus vector types. This, in my opinion, rep- sents a unique added value of this volume, which should help our work resist the passage of time, to which books such as this are particularly sensitive.

Gene Therapy for Neurological Disorders

Fredric P. Manfredsson
Gene Therapy for Neurological Disorders Book PDF

Author : Fredric P. Manfredsson
Publisher : Humana
Page : 0 pages
File Size : 54,8 Mb
Release : 2015-11-26
Category : Medical
ISBN : 1493932705

Get Book

Gene Therapy for Neurological Disorders by Fredric P. Manfredsson Pdf

This volume provides a clear and detailed roadmap of how to design and execute a gene therapy experiment in order to obtain consistent results. Chapters in this book disseminate bits of unknown information that are important to consider during the course of experimentation and will answer questions such as: What delivery vehicle do you use?; How will you ensure that your vector retains stability?; What expression system best fits your needs?; What route will you choose to deliver your gene therapy agent?; How will you model the neurodegenerative disorder that you aim to investigate and what are the proven methods to treat these disorders in preclinical models? Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls. Authoritative and thorough, Gene Therapy for Neurological Disorders: Methods and Protocols, is a compilation of protocols and instructive chapters intended to give researchers, clinicians, and students of all levels, a foundation upon which future gene therapy experiments can be designed.

Cancer Gene Therapy

David T. Curiel,Joanne T. Douglas
Cancer Gene Therapy Book PDF

Author : David T. Curiel,Joanne T. Douglas
Publisher : Springer Science & Business Media
Page : 490 pages
File Size : 43,6 Mb
Release : 2007-11-03
Category : Medical
ISBN : 9781592597857

Get Book

Cancer Gene Therapy by David T. Curiel,Joanne T. Douglas Pdf

A complete introduction and guide to the latest developments in cancer gene therapy-from bench to bedside. The authors comprehensively review the anticancer genes and gene delivery methods currently available for cancer gene therapy, including the transfer of genetic material into the cancer cells, stimulation of the immune system to recognize and eliminate cancer cells, and the targeting of the nonmalignant stromal cells that support their growth. They also thoroughly examine the advantages and limitations of the different therapies and detail strategies to overcome obstacles to their clinical implementation. Topics of special interest include vector-targeting techniques, the lessons learned to date from clinical trials of cancer gene therapy, and the regulatory guidelines for future trials. Noninvasive techniques to monitor the extent of gene transfer and disease regression during the course of treatment are also discussed.

Cardiac Gene Therapy

Kiyotake Ishikawa
Cardiac Gene Therapy Book PDF

Author : Kiyotake Ishikawa
Publisher : Springer Nature
Page : 342 pages
File Size : 43,9 Mb
Release : 2022-08-30
Category : Science
ISBN : 9781071627075

Get Book

Cardiac Gene Therapy by Kiyotake Ishikawa Pdf

This second edition volume expands on the previous edition with updated techniques and discussions on topics such as gene suppression, editing, and reprogramming; cardiac gene therapy vectors and promoters; cardiac gene delivery methods; pulmonary hypertension; and patient screening and measuring the efficacy of cardiac gene therapy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Cardiac Gene Therapy: Methods and Protocols, Second Edition is a valuable tool for researchers in cardiology who are conducting gene therapy research. This book will lead to further advancements and successful clinical translations in the field. Chapter “Direct Reprogramming of Adult Human Cardiac Fibroblasts into Induced Cardiomyocytes Using miRcombo” is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Gene Therapy of Cancer

Wolfgang Walther
Gene Therapy of Cancer Book PDF

Author : Wolfgang Walther
Publisher : Springer Nature
Page : 342 pages
File Size : 41,5 Mb
Release : 2022-06-22
Category : Medical
ISBN : 9781071624418

Get Book

Gene Therapy of Cancer by Wolfgang Walther Pdf

This third edition provides new and updated chapters on gene therapeutic strategies of cancer. Chapters guide readers through suicide and oncolytic gene therapy, gene replacement and gene suppression therapy, vector development and refinement, immunogene therapy, TCR and CAR engineering, tumor vaccination using DNA or RNA vaccines, and antitumoral immune stimulation at different levels. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Gene Therapy of Cancer: Methods and Protocols, Third Edition aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge.

Gene Therapy - From Laboratory To The Clinic

Kam M Hui
Gene Therapy From Laboratory To The Clinic Book PDF

Author : Kam M Hui
Publisher : World Scientific
Page : 289 pages
File Size : 40,8 Mb
Release : 1994-11-29
Category : Science
ISBN : 9789814502382

Get Book

Gene Therapy - From Laboratory To The Clinic by Kam M Hui Pdf

With advances in our understanding of the molecular biology of human diseases and the development of efficient gene transfer techniques, the treatment of such diseases as cancer and infectious disease using gene therapy has progressed from a distant prospect to a distinct possibility in a very short time. The development of gene transfer methods which are suitable for different forms of therapy has been a major topic of research over the past several years. A common goal of this research has been to achieve the efficient delivery of genes into cells. The successful implementation of gene transfer as a cure for diseases, however, will continue to require the translation of preclinical studies in gene therapy into effective clinical protocols. This volume outlines the latest developments in cancer treatment using various gene delivery systems, which include cytokine gene transfer, the delivery of anti-ras DNA by retroviral vector and the injection of allogeneic HLA DNA via liposomes. Several of these molecular approaches have recently been approved by the US FDA as human clinical trial protocols in order to assess their therapeutic efficiency and safety for cancer treatment. Further developments in recombinant DNA technology within this field should ultimately lead to dramatic improvements in the practice of medicine.

Oversight and Review of Clinical Gene Transfer Protocols

Institute of Medicine,Board on Health Sciences Policy,Committee on the Independent Review and Assessment of the Activities of the NIH Recombinant DNA Advisory Committee
Oversight and Review of Clinical Gene Transfer Protocols Book PDF

Author : Institute of Medicine,Board on Health Sciences Policy,Committee on the Independent Review and Assessment of the Activities of the NIH Recombinant DNA Advisory Committee
Publisher : National Academies Press
Page : 78 pages
File Size : 43,7 Mb
Release : 2014-03-27
Category : Medical
ISBN : 9780309296656

Get Book

Oversight and Review of Clinical Gene Transfer Protocols by Institute of Medicine,Board on Health Sciences Policy,Committee on the Independent Review and Assessment of the Activities of the NIH Recombinant DNA Advisory Committee Pdf

Gene transfer research is a rapidly advancing field that involves the introduction of a genetic sequence into a human subject for research or diagnostic purposes. Clinical gene transfer trials are subject to regulation by the U.S. Food and Drug Administration (FDA) at the federal level and to oversight by institutional review boards (IRBs) and institutional biosafety committees (IBCs) at the local level before human subjects can be enrolled. In addition, at present all researchers and institutions funded by the National Institutes of Health (NIH) are required by NIH guidelines to submit human gene transfer protocols for advisory review by the NIH Recombinant DNA Advisory Committee (RAC). Some protocols are then selected for individual review and public discussion. Oversight and Review of Clinical Gene Transfer Protocols provides an assessment of the state of existing gene transfer science and the current regulatory and policy context under which research is investigated. This report assesses whether the current oversight of individual gene transfer protocols by the RAC continues to be necessary and offers recommendations concerning the criteria the NIH should employ to determine whether individual protocols should receive public review. The focus of this report is on the standards the RAC and NIH should use in exercising its oversight function. Oversight and Review of Clinical Gene Transfer Protocols will assist not only the RAC, but also research institutions and the general public with respect to utilizing and improving existing oversight processes.