Gene Transfer And Expression In Mammalian Cells

Author : S.C. Makrides
Publisher : Gulf Professional Publishing
Page : 730 pages
File Size : 40,5 Mb
Release : 2003-10-24
Category : Science
ISBN : 0444513701

Get Book

Gene Transfer and Expression in Mammalian Cells by S.C. Makrides Pdf

This volume provides a broad, state-of-the-art coverage of diverse technical topics in gene expression in mammalian cells, including the development of vectors for production of proteins in cultured cells, in transgenic animals, vaccination, and gene therapy; progress in methods for the transfer of genes into mammalian cells and the optimization and monitoring of gene expression; advances in our understanding and manipulation of cellular biochemical pathways that have a quantitative and qualitative impact on mammalian gene expression; and the large-scale production and purification of proteins from cultured cells.

Author : William C. Heiser
Publisher : Springer Science & Business Media
Page : 303 pages
File Size : 44,7 Mb
Release : 2008-02-03
Category : Science
ISBN : 9781592596492

Get Book

Gene Delivery to Mammalian Cells by William C. Heiser Pdf

Experienced researchers describe in step-by-step detail methods that have proven most useful in delivering genes to mammalian cells. Volume 1 focuses on gene delivery by a variety of chemical and physical methods, including ultrasound, biolistics, peptides, PNA clamps, liposomes, microinjection, electroporation, particle bombardment, dendrimers, and hydrodynamics. Volume 2 details procedures for delivering genes to cells in vitro and in vivo, including the use of lentiviral vectors.

Author : Edward J. Murray
Publisher : Springer Science & Business Media
Page : 417 pages
File Size : 40,5 Mb
Release : 2008-02-02
Category : Medical
ISBN : 9781592594948

Get Book

Gene Transfer and Expression Protocols by Edward J. Murray Pdf

Biology is the study of living things. The classical approach might be described as holistic and descriptive, whereas the modern molecular - proach aims to be investigative, reductionist, and mechanistic . Genes contain all the information for the structure of all living things ; thus, the understanding of how genes are regulated is an important step toward understanding the nature of living things. The study of gene regulation has been made more tractable by the design of simple expe- mental models in which a single gene can be isolated from the milieu of the organism. The new science of molecular biology has introduced techniques that permit the design of such experimental models. In - sence, the genome of the organism is dissected in such a manner that specific genes may now be introduced into an appropriate cell line . Subsequent analysis of the proteins expressed from the genes under study results in the identification of the regulatory DNA sequences .

Author : Michael Kriegler
Publisher : Springer
Page : 248 pages
File Size : 51,6 Mb
Release : 1990-06-18
Category : Science
ISBN : 9781349118915

Get Book

Gene Transfer and Expression by Michael Kriegler Pdf

A practical manual of protocols for achieving expression of foreign genes in mammalian cells. It includes some very new techniques such as PCR-based expression. The author gives a theoretical introduction to the protocols and compares the strengths and weaknesses.

Author : Jeffrey H. Miller,Michele P. Calos
Publisher : Unknown
Page : 188 pages
File Size : 40,9 Mb
Release : 1987
Category : Science
ISBN : UOM:39015012637735

Get Book

Gene Transfer Vectors for Mammalian Cells by Jeffrey H. Miller,Michele P. Calos Pdf

Author : Richard Twyman
Publisher : Taylor & Francis
Page : 299 pages
File Size : 55,8 Mb
Release : 2004-12-01
Category : Science
ISBN : 9781135322793

Get Book

Gene Transfer to Animal Cells by Richard Twyman Pdf

Gene transfer to animal cells was first achieved more than thirty years ago. Since then, transformation technology has developed rapidly, resulting in a multitude of techniques for cell transformation and the creation of transgenic animals. As with any expanding technology, it becomes difficult to keep track of all the developments and to find a concise and comprehensive source of information that explains all the underlying principles. Gene Transfer to Animals Cells addresses this problem by describing the principles behind gene transfer technologies, how gene expression is controlled in animal cells and how advanced strategies can be used to add, exchange or delete sequences from animal genomes in a conditional manner. A final chapter provides an overview of all the applications of animal cell transformation in farming, medicine and research.

Author : Theodore Friedmann,John J. Rossi
Publisher : CSHL Press
Page : 769 pages
File Size : 54,8 Mb
Release : 2007
Category : Gene Transfer Techniques
ISBN : 9780879697655

Get Book

Gene Transfer by Theodore Friedmann,John J. Rossi Pdf

Understanding gene function and regulation requires rigorous testing in live cells and organisms. Recent advances have provided a variety of new strategies for delivering DNA and RNA into cells and probing their expression, as well as new clinical applications that rely upon the introduction of genetic material. The vast number of available techniques for clinical and laboratory research often makes selecting the optimal method a difficult process. Gene Transfer: Delivery and Expression of DNA and RNA provides the first comprehensive guide to technical approaches for delivering nucleic acids into cells and organisms and of ensuring (even manipulating) appropriate expression. The detailed, step-by-step protocols cover a variety of methods, both well established and newly evolving. These include viral and nonviral methods of gene delivery, transgenic approaches, strategies for the regulation of transgene expression, and modification of the host response. The introductory matter to each chapter includes concise technical and theoretical discussions with considerations for selection of the appropriate system and strategies for delivery.

Author : R. Kucherlapati
Publisher : Springer Science & Business Media
Page : 447 pages
File Size : 51,7 Mb
Release : 2012-12-06
Category : Medical
ISBN : 9781468451672

Get Book

Gene Transfer by R. Kucherlapati Pdf

Genetic analysis of microbial systems provided us with the foundation for un derstanding gene structure, expression, and regulation. It was long felt that the ability to generate mutants and conduct genetic studies in mammalian systems would prove to be equally useful. However, genetic analysis based on sexual systems is difficult in mammals because of the long generation times and the inability to perform controlled matings. As a result, genetic analysis of mam malian systems had to await the development of parasexual systems. This book is an attempt to bring together descriptions of a number of these parasexual systems. A common theme of all the parasexual systems is the transfer of genetic information from a defined source into a specific cell type. This volume deals with a number of methods of gene transfer into mammalian cells. The early methods of gene transfer involved transfer of relatively large amounts of genetic information. These include somatic cell hybridization, microcell fusion, and chromosome transfer, which constitute the first part of this book. Each of these methods has already proven to be of enormous value in arriving at a genetic understanding of the mammalian genome. Development of recombinant DNA methods, and the ability to introduce purified DNA into mammalian cells, has had a significant impact on our ability to dissect important aspects of mammalian gene expression and regulation. The second part of this book deals with gene transfer systems involving defined nucleic acid sequences.

Author : Jon A. Wolff
Publisher : Springer Science & Business Media
Page : 431 pages
File Size : 55,7 Mb
Release : 2012-12-06
Category : Medical
ISBN : 9781468468229

Get Book

Gene Therapeutics by Jon A. Wolff Pdf

During the first half century of genetics, coinciding with the first half of this cen tury, geneticists dreamt of the repair of genetic disease by altering or replacing defective genes. H. J. Muller wrote of the great advantages of mutations, "nanoneedles" in his apt term, for delicately probing physiological and chemical processes. In the same spirit, genes could be used to provide treatments of needle point delicacy. Yet, during this period no realistic possibility appeared; it remained but a dream. The situation changed abruptly at the half century. Microbial genetics and its offshoot, cell culture genetics, provided the route. Pneumococcus transformation showed that exogenous DNA could become a permanent part of the genome; yet attempts to reproduce this in animals produced a few tantalizing hints of success, but mostly failures. Transduction, using a virus as mediator, offered a better op portunity. The fITSt reproducible in vivo gene therapy in a whole animal came in 1981. This was in Drosophila, with a transposable element as carrier. Flies were "cured" of a mutant eye color by incorporation of the normal allele, and the effect was transmissible, foreshadowing not only somatic, but germ line gene therapy. At the same time, retroviruses carrying human genes were found to be ex tremely efficient in transferring their contents to the chromosomes of cultured cells.

Author : William C. Heiser
Publisher : Humana Press
Page : 566 pages
File Size : 52,9 Mb
Release : 2003-10-28
Category : Science
ISBN : 1588290956

Get Book

Gene Delivery to Mammalian Cells by William C. Heiser Pdf

The efficiency of delivering DNA into mammalian cells has increased t- mendously since DEAE dextran was first shown to be capable of enhancing transfer of RNA into mammalian cells in culture. Not only have other chemical methods been developed and refined, but also very efficient physical and viral delivery methods have been established. The technique of introducing DNA into cells has developed from transfecting tissue culture cells to delivering DNA to specific cell types and organs in vivo. Moreover, two important areas of biology—assessment of gene function and gene therapy—require succe- ful DNA delivery to cells, driving the practical need to increase the efficiency and efficacy of gene transfer both in vitro and in vivo. TM These two volumes of the Methods in Molecular Biology series, Gene Del- ery to Mammalian Cells, are designed as a compendium of those techniques that have proven most useful in the expanding field of gene transfer in mammalian cells. It is intended that these volumes will provide a thorough background on chemical, physical, and viral methods of gene delivery, a synopsis of the myriad techniques currently available to introduce genes into mammalian cells, as well as a practical guide on how to accomplish this. It is my expectation that it will be useful to the novice in the field as well as to the scientist with expertise in gene delivery.

Author : Anonim
Publisher : Academic Press
Page : 379 pages
File Size : 42,7 Mb
Release : 1994-10-18
Category : Science
ISBN : 0080859410

Get Book

Protein Expression in Animal Cells by Anonim Pdf

Critically acclaimed for more than 25 years, the Methods in Cell Biology series provides an indispensable tool for the researcher. Each volume is carefully edited by experts to contain state-of-the-art reviews and step-by-step protocols. Techniques are described completely so that methods are made accessible to users. Describes both well-established and novel recombinant vector systems for expression of proteins Presents methods for efficient delivery of recombinant genes into differentiated cells, tissues, and whole animals Covers high-level and inducible systems, plus assays for protein expression Provides beginning and advanced investigators and students with the information they need to choose the optimal viral or plasmid system for their protein Practical, benchtop-style presentation works in lab and in the classroom

Author : William C. Heiser
Publisher : Humana Press
Page : 0 pages
File Size : 55,8 Mb
Release : 2010-11-10
Category : Science
ISBN : 1617373494

Get Book

Gene Delivery to Mammalian Cells by William C. Heiser Pdf

Highly experienced researchers describe in step-by-step detail methods that have proven most useful in delivering genes to mammalian cells. Volume 1: Nonviral Gene Transfer Techniques focuses on gene delivery by a variety of chemical and physical methods, including ultrasound, biolistics, peptides, PNA clamps, liposomes, microinjection, electroporation, particle bombardment, dendrimers, and hydrodynamics. An accompanying volume, Volume 2: Viral Gene Transfer Techniques, details procedures for delivering genes to cells in vitro and in vivo, including the use of lentiviral vectors.

Author : Bruce Alberts
Publisher : Unknown
Page : 0 pages
File Size : 49,5 Mb
Release : 2002
Category : Cytology
ISBN : 0815332181

Get Book

Molecular Biology of The Cell by Bruce Alberts Pdf

Author : Paulina Balbas,Argelia Lorence
Publisher : Springer Science & Business Media
Page : 505 pages
File Size : 46,5 Mb
Release : 2008-02-04
Category : Science
ISBN : 9781592597741

Get Book

Recombinant Gene Expression by Paulina Balbas,Argelia Lorence Pdf

Since newly created beings are often perceived as either wholly good or bad, the genetic alteration of living cells impacts directly on a symbolic meaning deeply imbedded in every culture. During the earlier years of gene expression research, te- nological applications were confined mainly to academic and industrial laboratories, and were perceived as highly beneficial since molecules that were previously unable to be separated or synthesized became accessible as therapeutic agents. Such were the success stories of hormones, antibodies, and vaccines produced in the bacterium Escherichia coli. Originally this bacterium gained fame among humans for being an unwanted host in the intestine, or worse yet, for being occasionally dangerous and pathogenic. H- ever, it was easily identified in contaminated waters during the 19th century, thus becoming a clear indicator of water pollution by human feces. Tamed, cultivated, and easily maintained in laboratories, its fast growth rate and metabolic capacity to adjust to changing environments fascinated the minds of scientists who studied and modeled such complex phenomena as growth, evolution, genetic exchange, infection, survival, adaptation, and further on—gene expression. Although at the lower end of the complexity scale, this microbe became a very successful model system and a key player in the fantastic revolution kindled by the birth of recombinant DNA technology.

Author : Carl William Anderson
Publisher : Unknown
Page : 398 pages
File Size : 45,9 Mb
Release : 1978
Category : Bacterial transformation
ISBN : WISC:89034758664

Get Book

Genetic Interaction and Gene Transfer by Carl William Anderson Pdf