Gene Transfer To Animal Cells

Author : Richard Twyman
Publisher : Taylor & Francis
Page : 299 pages
File Size : 47,7 Mb
Release : 2004-12-01
Category : Science
ISBN : 9781135322793

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Gene Transfer to Animal Cells by Richard Twyman Pdf

Gene transfer to animal cells was first achieved more than thirty years ago. Since then, transformation technology has developed rapidly, resulting in a multitude of techniques for cell transformation and the creation of transgenic animals. As with any expanding technology, it becomes difficult to keep track of all the developments and to find a concise and comprehensive source of information that explains all the underlying principles. Gene Transfer to Animals Cells addresses this problem by describing the principles behind gene transfer technologies, how gene expression is controlled in animal cells and how advanced strategies can be used to add, exchange or delete sequences from animal genomes in a conditional manner. A final chapter provides an overview of all the applications of animal cell transformation in farming, medicine and research.

Author : S.C. Makrides
Publisher : Gulf Professional Publishing
Page : 730 pages
File Size : 43,6 Mb
Release : 2003-10-24
Category : Science
ISBN : 0444513701

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Gene Transfer and Expression in Mammalian Cells by S.C. Makrides Pdf

This volume provides a broad, state-of-the-art coverage of diverse technical topics in gene expression in mammalian cells, including the development of vectors for production of proteins in cultured cells, in transgenic animals, vaccination, and gene therapy; progress in methods for the transfer of genes into mammalian cells and the optimization and monitoring of gene expression; advances in our understanding and manipulation of cellular biochemical pathways that have a quantitative and qualitative impact on mammalian gene expression; and the large-scale production and purification of proteins from cultured cells.

Author : Jac A. Nickoloff
Publisher : Springer Science & Business Media
Page : 367 pages
File Size : 45,9 Mb
Release : 2008-02-03
Category : Science
ISBN : 9781592595358

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Animal Cell Electroporation and Electrofusion Protocols by Jac A. Nickoloff Pdf

The ability to introduce macromolecules into animal cells, includ ing DNA, RNA, proteins, and other bioactive compounds has facili tated a broad range of biological studies, from biochemistry and biophysics to molecular biology, cell biology, and whole animal stud ies. Gene transfer technology in particular will continue to play an essential role in studies aimed at improving our understanding of the relationships between the gene structure and function, and it has impor tant practical applications in both biotechnology and biomedicine, as evidenced by the current intense interest in gene therapy. Although DNA and other macromolecules may be introduced into cells by a variety of methods, including chemical treatments and microinjection, el- troporation has proven to be simpler to perform, more efficient, and effective with a wider variety of cell types than other techniques. The early and broad success of electric field-mediated DNA transfer soon prompted researchers to investigate electroporation for transferring other types of molecules into cells, including RNA, enzymes, antibodies, and analytic dyes. Animal Cell Electroporation and Electrofusion Protocols begins with three chapters that describe the theoretical and practical aspects of electroporation, including a review of the commercially available instrumentation. These introductory chapters will be of particular inter est to those new to electric field technologies and to those developing protocols for as yet untested species or cell types. Nineteen chapters follow that present well-tested protocols for electroporation of proteins and DNA into insect, fish, and mammalian cells.

Author : A. Pühler
Publisher : Unknown
Page : 196 pages
File Size : 47,8 Mb
Release : 1993
Category : Animal genetic engineering
ISBN : STANFORD:36105016207743

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Genetic Engineering of Animals by A. Pühler Pdf

Author : Paul F. Lurquin
Publisher : Springer Science & Business Media
Page : 281 pages
File Size : 46,6 Mb
Release : 2012-12-06
Category : Medical
ISBN : 9781468444933

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Genetic Engineering in Eukaryotes by Paul F. Lurquin Pdf

This book includes the proceedings of a NATO Advanced Study Institute held at Washington State University, Pullman, Washington from July 26 until August 6, 1982. Although genetic engineering in eukaryotes is best developed in yeast and mammalian cells, the reader will find that some emphasis has been put on plant systems. Indeed, it was our position that the development of plant cell genetic transformation would benefit from the interactions between a comparatively smaller number of fungal and animal cell experts and a larger number of plant cell specialists representing various aspects of plant molecular genetic research. On the other hand, it is clear that the ultimate achievements of plant genetic engineering will have a tremendous ~pact on, among other things, food production without generating the problems of ethics encountered when one contempla-tes the genetic modification of human beings. Therefore, this slight bias in favor of the plant kingdom simply reflects our belief that a "second green revolution" will benefit mankind to a greater extent than any other kind of genetic engine ering. The keynote lecture of the Institute was delivered by Dr. John Slaughter, Director of the National Science Foundation, whom we deeply thank for his words of encouragement and commitment to the genetic manipulation of plants.

Author : Jon A. Wolff
Publisher : Springer Science & Business Media
Page : 431 pages
File Size : 43,7 Mb
Release : 2012-12-06
Category : Medical
ISBN : 9781468468229

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Gene Therapeutics by Jon A. Wolff Pdf

During the first half century of genetics, coinciding with the first half of this cen tury, geneticists dreamt of the repair of genetic disease by altering or replacing defective genes. H. J. Muller wrote of the great advantages of mutations, "nanoneedles" in his apt term, for delicately probing physiological and chemical processes. In the same spirit, genes could be used to provide treatments of needle point delicacy. Yet, during this period no realistic possibility appeared; it remained but a dream. The situation changed abruptly at the half century. Microbial genetics and its offshoot, cell culture genetics, provided the route. Pneumococcus transformation showed that exogenous DNA could become a permanent part of the genome; yet attempts to reproduce this in animals produced a few tantalizing hints of success, but mostly failures. Transduction, using a virus as mediator, offered a better op portunity. The fITSt reproducible in vivo gene therapy in a whole animal came in 1981. This was in Drosophila, with a transposable element as carrier. Flies were "cured" of a mutant eye color by incorporation of the normal allele, and the effect was transmissible, foreshadowing not only somatic, but germ line gene therapy. At the same time, retroviruses carrying human genes were found to be ex tremely efficient in transferring their contents to the chromosomes of cultured cells.

Author : Suzanne Nanis
Publisher : Unknown
Page : 18 pages
File Size : 48,8 Mb
Release : 1989
Category : Animals
ISBN : MINN:319510029673354

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Biotechnology by Suzanne Nanis Pdf

Author : Anonim
Publisher : Academic Press
Page : 379 pages
File Size : 43,7 Mb
Release : 1994-10-18
Category : Science
ISBN : 0080859410

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Protein Expression in Animal Cells by Anonim Pdf

Critically acclaimed for more than 25 years, the Methods in Cell Biology series provides an indispensable tool for the researcher. Each volume is carefully edited by experts to contain state-of-the-art reviews and step-by-step protocols. Techniques are described completely so that methods are made accessible to users. Describes both well-established and novel recombinant vector systems for expression of proteins Presents methods for efficient delivery of recombinant genes into differentiated cells, tissues, and whole animals Covers high-level and inducible systems, plus assays for protein expression Provides beginning and advanced investigators and students with the information they need to choose the optimal viral or plasmid system for their protein Practical, benchtop-style presentation works in lab and in the classroom

Author : Leda Castilho,Angela Moraes,Elisabeth Augusto,Mike Butler
Publisher : Taylor & Francis
Page : 506 pages
File Size : 41,8 Mb
Release : 2008-03-01
Category : Medical
ISBN : 9781134099726

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Animal Cell Technology by Leda Castilho,Angela Moraes,Elisabeth Augusto,Mike Butler Pdf

Animal Cell Technology: from Biopharmaceuticals to Gene Therapy provides a comprehensive insight into biological and engineering concepts related to mammalian and insect cell technology, as well as an overview of the applications of animal cell technology. Part 1 of the book covers the Fundamentals upon which this technology is based and covers the science underpinning the technology. Part 2 covers the Applications from the production of therapeutic proteins to gene therapy. The authors of the chapters are internationally-recognized in the field of animal cell culture research and have extensive experience in the areas covered in their respective chapters.

Author : Carl A. Pinkert
Publisher : Gulf Professional Publishing
Page : 635 pages
File Size : 43,5 Mb
Release : 2002-11-18
Category : Medical
ISBN : 9780125571661

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Transgenic Animal Technology by Carl A. Pinkert Pdf

A transgenic animal is one that is genetically modified to carry genes from another species. Transgenic species can be raised to carry potentially useful genes from a variety of species. While the topics of genetic engineering and cloning are controversial, the reality is that these technologies offer tremendous benefits to society - from offering a framework for developing and screening medical therapies, to enhancing the safety and nutrition of the food we eat. One potential application of research into transgenic animal technology is the creation of domestic animals genetically designed to express a certain human disease and therefore serve as models for the study and treatment of human illnesses. Although many mouse models of human diseases are available today, such models in large domestic animals physiologically more similar to humans are sparse and critically needed. Further research in this field will undoubtedly uncover many more direct and indirect benefits of this technology. Transgenic animal technologies and the ability to introduce functional genes into animals have revolutionized our ability to address complex biomedical and biological questions. This well-illustrated handbook covers the technical aspects of gene transfer - from molecular methods to whole animal considerations - for important laboratory and domestic animal species. It describes methodologies as employed by leading laboratories and is a key resource for researchers, as well as a tool for training technicians and students. This second edition incorporates updates on a variety of genetic engineering technologies ranging from microinjection and ES cell transfer to nuclear transfer in a broad range of animal modeling systems. Contains a comprehensive collection of transgenic animal and gene transfer methods Discusses background and introduction to techniques and animal systems Teaches practical step-by-step protocols New section on analysis

Author : Daniel Scherman
Publisher : World Scientific
Page : 556 pages
File Size : 44,7 Mb
Release : 2013-12-10
Category : Science
ISBN : 9781783263141

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Advanced Textbook On Gene Transfer, Gene Therapy And Genetic Pharmacology: Principles, Delivery And Pharmacological And Biomedical Applications Of Nucleotide-based Therapies by Daniel Scherman Pdf

This unique advanced textbook provides a clear and comprehensive description of the field of gene delivery, gene therapy and genetic pharmacology, with descriptions of the main gene transfer vectors and a set of selected therapeutic applications, along with safety considerations.The use of gene transfer is exponentially growing in the scientific and medical communities for day-to-day cell biology experiments and swift development of revolutionary gene therapy strategies. In this advanced textbook, more than 25 leading scientists, world-renowned in their respective fields, come together to provide a clear and comprehensive description of gene delivery, gene therapy and genetic pharmacology.This educational introduction to the main gene transfer vectors and selected therapeutic applications provides the background material needed to further explore the subject as well as relevant research literature. It will thus be invaluable to Master, PhD or MD students, post-doctoral scientists or medical doctors, as well as any scientist wishing to deliver a gene or synthetic nucleotide, or develop a gene therapy strategy. Furthermore, the textbook's simple and synthetic content will be of value to any reader interested in the biological and medical revolution derived from the elucidation of the human genome.

Author : Harry Johnson
Publisher : States Academic Press
Page : 244 pages
File Size : 43,6 Mb
Release : 2021-11-16
Category : Science
ISBN : 1639890483

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Animal Cell Biotechnology by Harry Johnson Pdf

The branch of biology which makes use of living organisms to create new products is termed as biotechnology. The application of biotechnology for studying animal cells and cell culture is termed as animal cell biotechnology. Some of the key areas of study under this field are sequencing of animal genomes, recombinant DNA technology, identification of genes, regulation of genes, molecular markers and diagnostics. The animals which are genetically altered to have specific characteristics are known as transgenic animals. They are produced using a variety of techniques such as nuclear transfer, DNA microinjection, sperm mediated gene transfer and artificial chromosomes. Animal cell biotechnology finds application across various fields such as food production, organ transplant, pharmaceuticals, animal forensics and animal breeding. Animal cell biotechnology is an upcoming field of science that has undergone rapid development over the past few decades. Most of the topics introduced herein cover new techniques and the applications of animal cell biotechnology. Those in search of information to further their knowledge will be greatly assisted by this book.

Author : Robert D. Warmbrodt
Publisher : Unknown
Page : 76 pages
File Size : 45,6 Mb
Release : 1992
Category : Animal biotechnology
ISBN : MINN:31951D00834477I

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Gene Transfer in Animal Systems by Robert D. Warmbrodt Pdf

Author : William C. Heiser
Publisher : Humana Press
Page : 301 pages
File Size : 52,8 Mb
Release : 2003-10-28
Category : Science
ISBN : 1588290867

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Gene Delivery to Mammalian Cells by William C. Heiser Pdf

Highly experienced researchers describe in step-by-step detail methods that have proven most useful in delivering genes to mammalian cells. Volume 1: Nonviral Gene Transfer Techniques focuses on gene delivery by a variety of chemical and physical methods, including ultrasound, biolistics, peptides, PNA clamps, liposomes, microinjection, electroporation, particle bombardment, dendrimers, and hydrodynamics. An accompanying volume, Volume 2: Viral Gene Transfer Techniques, details procedures for delivering genes to cells in vitro and in vivo, including the use of lentiviral vectors.

Author : Edward J. Murray
Publisher : Springer Science & Business Media
Page : 417 pages
File Size : 47,9 Mb
Release : 2008-02-02
Category : Medical
ISBN : 9781592594948

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Gene Transfer and Expression Protocols by Edward J. Murray Pdf

Biology is the study of living things. The classical approach might be described as holistic and descriptive, whereas the modern molecular - proach aims to be investigative, reductionist, and mechanistic . Genes contain all the information for the structure of all living things ; thus, the understanding of how genes are regulated is an important step toward understanding the nature of living things. The study of gene regulation has been made more tractable by the design of simple expe- mental models in which a single gene can be isolated from the milieu of the organism. The new science of molecular biology has introduced techniques that permit the design of such experimental models. In - sence, the genome of the organism is dissected in such a manner that specific genes may now be introduced into an appropriate cell line . Subsequent analysis of the proteins expressed from the genes under study results in the identification of the regulatory DNA sequences .