Genetically Incorporated Non Canonical Amino Acids

Author : Yu-Hsuan Tsai,Simon J. Elsässer
Publisher : Springer Nature
Page : 287 pages
File Size : 52,6 Mb
Release : 2023-06-05
Category : Science
ISBN : 9781071632512

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Genetically Incorporated Non-Canonical Amino Acids by Yu-Hsuan Tsai,Simon J. Elsässer Pdf

This detailed volume explores non-canonical amino acids (ncAAs) through their site-specific incorporation by genetic code expansion (GCE). The collection provides a broad resource of methods for implementing GCE in E. coli, mammalian cells, and animals, highlighting specific applications ranging from fluorescence labeling to photocontrol and the study of protein post-translational modification. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Genetically Incorporated Non-Canonical Amino Acids: Methods and Protocols serves as an ideal source of methodologies that can be adapted and extended, migrated to different model systems, and combined in new ways to help explore a wide range of biological questions and to augment industrial and pharmaceutical protein engineering.

Author : Edward A. Lemke
Publisher : Unknown
Page : 411 pages
File Size : 55,9 Mb
Release : 2018
Category : Amino acids
ISBN : 1493975749

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Noncanonical Amino Acids by Edward A. Lemke Pdf

Author : Anonim
Publisher : Academic Press
Page : 350 pages
File Size : 49,7 Mb
Release : 2009-07-24
Category : Science
ISBN : 0080921639

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Non-Natural Amino Acids by Anonim Pdf

By combining the tools of organic chemistry with those of physical biochemistry and cell biology, Non-Natural Amino Acids aims to provide fundamental insights into how proteins work within the context of complex biological systems of biomedical interest. The critically acclaimed laboratory standard for 40 years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. With more than 400 volumes published, each Methods in Enzymology volume presents material that is relevant in today's labs -- truly an essential publication for researchers in all fields of life sciences. Demonstrates how the tools and principles of chemistry combined with the molecules and processes of living cells can be combined to create molecules with new properties and functions found neither in nature nor in the test tube Presents new insights into the molecular mechanisms of complex biological and chemical systems that can be gained by studying the structure and function of non-natural molecules Provides a "one-stop shop" for tried and tested essential techniques, eliminating the need to wade through untested or unreliable methods

Author : Gustavo Fuertes,Kensaku Sakamoto,Ned Budisa
Publisher : Frontiers Media SA
Page : 123 pages
File Size : 42,7 Mb
Release : 2023-11-01
Category : Science
ISBN : 9782832538029

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Exploring and Expanding the Protein Universe with Non-Canonical Amino Acids by Gustavo Fuertes,Kensaku Sakamoto,Ned Budisa Pdf

The site-specific incorporation of unnatural or non-canonical amino acids (ncAAs) into proteins is a universally important tool for systems bioengineering at the interface of chemistry, biology, and biotechnology. The synergistic use of ncAA and related technologies (e.g. Xeno nucleic acids) should enable: i) New opportunities to manipulate, design and elucidate protein structure, dynamics, and function. ii) A deeper understanding of natural and evolved translational systems and their importance for artificial biology. iii) The synthesis of novel biopolymers, creating a solid basis for synthetic cells, which is also an important technology in the production of new classes of medically relevant protein-based scaffolds. Research on reprogrammed protein translation has now reached an experimental and intellectual maturity: more than 200 ncAA (i.e. more than ten times larger variety than standard amino acids) have been introduced into proteins using different routes: genetic code expansion (GCE), selective pressure incorporation (SPI), chemical mutagenesis, protein semi-synthesis, and peptide synthesis.

Author : Loredano Pollegioni,Stefano Servi
Publisher : Humana Press
Page : 409 pages
File Size : 42,8 Mb
Release : 2011-10-05
Category : Science
ISBN : 1617793302

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Unnatural Amino Acids by Loredano Pollegioni,Stefano Servi Pdf

Even though they are present in nature, non-proteinogenic amino acids are usually defined as unnatural or non-natural. Beside their structural diversity, interest in these compounds is due to their occurrence in nature, their biological properties, the analytical aspects, their use as probes, and their incorporation into peptides and proteins, among other reasons. Divided into five convenient sections, Unnatural Amino Acids: Methods and Protocols deals with enzymatic methods used to produce non-natural amino acids, aspects concerning the presence of unnatural amino acids in peptides with antimicrobial properties, genetic incorporation of unnatural amino acids into proteins (yeast and mammalian cells), and detection and quantification of D-amino acids and related enzymes. Written in the highly successful Methods in Molecular BiologyTM series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Unnatural Amino Acids: Methods and Protocols serves as an ideal guide for scientists and contributes to directing the attention of researchers to the many fields of growing scientific interest in non-natural amino acids.

Author : Nediljko Budisa
Publisher : John Wiley & Sons
Page : 312 pages
File Size : 40,8 Mb
Release : 2006-05-12
Category : Science
ISBN : 9783527607099

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Engineering the Genetic Code by Nediljko Budisa Pdf

The ability to introduce non-canonical amino acids in vivo has greatly expanded the repertoire of accessible proteins for basic research and biotechnological application. Here, the different methods and strategies to incorporate new or modified amino acids are explained in detail, including a lot of practical advice for first-time users of this powerful technique. Novel applications in protein biochemistry, genomics, biotechnology and biomedicine made possible by the expansion of the genetic code are discussed and numerous examples are given. Essential reading for all molecular life scientists who want to stay ahead in their research.

Author : Ingrid-Cristiana Gebura-Vreja
Publisher : Unknown
Page : 0 pages
File Size : 53,5 Mb
Release : 2015
Category : Electronic
ISBN : OCLC:934221355

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Non-canonical Amino Acids as Minimal Tags for Investigating Protein Organization and Turnover by Ingrid-Cristiana Gebura-Vreja Pdf

The burgeoning field of genetic code expansion provides new tools for specifically labeling proteins for a variety of applications. Clickable non-canonical amino acids (ncAA) have been refined for almost-quantitative and highly selective reactions with complementary probes. In my thesis work I have adapted genetic code expansion for two biological questions. First, I used ncAA incorporation and click reaction to test the effect of fluorescent protein (FP) tagging on the nanoscale organization of target proteins. Second, I used these tools to generate a genetically-encoded scheme for specifi ...

Author : Patrick O’Donoghue,Ilka Heinemann
Publisher : MDPI
Page : 188 pages
File Size : 40,9 Mb
Release : 2019-11-19
Category : Science
ISBN : 9783039217342

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Synthetic DNA and RNA Programming by Patrick O’Donoghue,Ilka Heinemann Pdf

Dear Colleagues, Synthetic biology is a broad and emerging discipline that capitalizes on recent advances in molecular biology, genetics, protein and RNA engineering and omics technologies. These technologies have transformed our ability to reveal the biology of the cell and the molecular basis of disease. This Special Issue on “Synthetic RNA and DNA Programming” features original research articles and reviews, highlighting novel aspects of basic molecular biology and the molecular mechanisms of disease that were uncovered by the application and development of novel synthetic biology-driven approaches.

Author : Dieter Söll,Uttam L. RajBhandary
Publisher : Unknown
Page : 572 pages
File Size : 50,9 Mb
Release : 1995
Category : Aminoacyl-tRNA synthetases
ISBN : 1555818331

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TRNA by Dieter Söll,Uttam L. RajBhandary Pdf

Author : Roland K. Hartmann,Albrecht Bindereif,Astrid Sch¿n,Eric Westhof
Publisher : John Wiley & Sons
Page : 1370 pages
File Size : 47,6 Mb
Release : 2015-06-22
Category : Science
ISBN : 9783527327768

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Handbook of RNA Biochemistry by Roland K. Hartmann,Albrecht Bindereif,Astrid Sch¿n,Eric Westhof Pdf

The second edition of a highly acclaimed handbook and ready reference. Unmatched in its breadth and quality, around 100 specialists from all over the world share their up-to-date expertise and experiences, including hundreds of protocols, complete with explanations, and hitherto unpublished troubleshooting hints. They cover all modern techniques for the handling, analysis and modification of RNAs and their complexes with proteins. Throughout, they bear the practising bench scientist in mind, providing quick and reliable access to a plethora of solutions for practical questions of RNA research, ranging from simple to highly complex. This broad scope allows the treatment of specialized methods side by side with basic biochemical techniques, making the book a real treasure trove for every researcher experimenting with RNA.

Author : Nick J Westwood,Adam Nelson
Publisher : Royal Society of Chemistry
Page : 339 pages
File Size : 43,5 Mb
Release : 2018-08-16
Category : Science
ISBN : 9781788015073

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Chemical and Biological Synthesis by Nick J Westwood,Adam Nelson Pdf

Synthetic chemistry plays a central role in many areas of chemical biology; utilising recent case studies, the goal of Chemical and Biological Synthesis is to highlight the full impact that the preparation of novel reagents can have in chemical biology. Covering the synthetic approaches that can be applied across the whole field of chemical biology, this book provides synthetic chemists with the broader context to which their work contributes and the biological questions that can be addressed through it. An ideal guide for postgraduate students and researchers in synthetic organic chemistry and chemical biology, Chemical and Biological Synthesis introduces synthetic techniques and methods to those who wish to incorporate synthesis for the first time in their biology-focused research programmes.

Author : Anonim
Publisher : ScholarlyEditions
Page : 279 pages
File Size : 46,5 Mb
Release : 2013-06-21
Category : Science
ISBN : 9781481690706

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Organelles—Advances in Research and Application: 2013 Edition by Anonim Pdf

Organelles—Advances in Research and Application: 2013 Edition is a ScholarlyEditions™ book that delivers timely, authoritative, and comprehensive information about Ribosomes. The editors have built Organelles—Advances in Research and Application: 2013 Edition on the vast information databases of ScholarlyNews.™ You can expect the information about Ribosomes in this book to be deeper than what you can access anywhere else, as well as consistently reliable, authoritative, informed, and relevant. The content of Organelles—Advances in Research and Application: 2013 Edition has been produced by the world’s leading scientists, engineers, analysts, research institutions, and companies. All of the content is from peer-reviewed sources, and all of it is written, assembled, and edited by the editors at ScholarlyEditions™ and available exclusively from us. You now have a source you can cite with authority, confidence, and credibility. More information is available at http://www.ScholarlyEditions.com/.

Author : W. Andy Tao,Ying Zhang
Publisher : John Wiley & Sons
Page : 448 pages
File Size : 47,9 Mb
Release : 2019-07-10
Category : Science
ISBN : 9781118970218

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Mass Spectrometry-Based Chemical Proteomics by W. Andy Tao,Ying Zhang Pdf

PROVIDES STRATEGIES AND CONCEPTS FOR UNDERSTANDING CHEMICAL PROTEOMICS, AND ANALYZING PROTEIN FUNCTIONS, MODIFICATIONS, AND INTERACTIONS—EMPHASIZING MASS SPECTROMETRY THROUGHOUT Covering mass spectrometry for chemical proteomics, this book helps readers understand analytical strategies behind protein functions, their modifications and interactions, and applications in drug discovery. It provides a basic overview and presents concepts in chemical proteomics through three angles: Strategies, Technical Advances, and Applications. Chapters cover those many technical advances and applications in drug discovery, from target identification to validation and potential treatments. The first section of Mass Spectrometry-Based Chemical Proteomics starts by reviewing basic methods and recent advances in mass spectrometry for proteomics, including shotgun proteomics, quantitative proteomics, and data analyses. The next section covers a variety of techniques and strategies coupling chemical probes to MS-based proteomics to provide functional insights into the proteome. In the last section, it focuses on using chemical strategies to study protein post-translational modifications and high-order structures. Summarizes chemical proteomics, up-to-date concepts, analysis, and target validation Covers fundamentals and strategies, including the profiling of enzyme activities and protein-drug interactions Explains technical advances in the field and describes on shotgun proteomics, quantitative proteomics, and corresponding methods of software and database usage for proteomics Includes a wide variety of applications in drug discovery, from kinase inhibitors and intracellular drug targets to the chemoproteomics analysis of natural products Addresses an important tool in small molecule drug discovery, appealing to both academia and the pharmaceutical industry Mass Spectrometry-Based Chemical Proteomics is an excellent source of information for readers in both academia and industry in a variety of fields, including pharmaceutical sciences, drug discovery, molecular biology, bioinformatics, and analytical sciences.

Author : Michael Ibba
Publisher : CRC Press
Page : 0 pages
File Size : 54,5 Mb
Release : 2005-04-01
Category : Science
ISBN : 1587061899

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The Aminoacyl-tRNA Synthetases by Michael Ibba Pdf

By virtue of their role as catalysts of the aminoacylation reaction, the aminoacyl-tRNA synthetases ensure that the first step of translation is performed quickly and accurately. In this volume of 36 separate chapters, the many facets of this ancient and ubiquitous family are reviewed, including their surprising structural diversity, enzymology, tRNA interaction properties, and curious alternative functions. These chapters illustrate the degree to which the aminoacyl-tRNA synthetases employ a variety of mechanisms to carry out both the standard functions related to the synthesis of aminoacylated tRNA for protein synthesis, as well as the surprising functions associated with amino acid biosynthesis, cytokine function, and even the processivity of DNA replication. Other chapters explore the regulation of their synthesis, their role in disease, and their prospects as targets for antibacterial therapeutics. This monograph will be a valuable resource for all scientists interested in the fundamentals of protein synthesis from both a basic research and clinical perspective, as well as the relation of translational components to the evolution of the genetic code.

Author : Olga Iranzo,Ana Cecília Roque
Publisher : Humana
Page : 289 pages
File Size : 40,6 Mb
Release : 2021-07-23
Category : Science
ISBN : 1071607227

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Peptide and Protein Engineering by Olga Iranzo,Ana Cecília Roque Pdf

This thorough book aims to present the methods that have enabled the success of peptides and proteins in a wide variety of applications. It opens with a section on chemical tools applied to the production or engineering of peptides and proteins, and concludes with a collection of chapters on biological approaches used to engineer structure and function in peptides and proteins. As a book in the Springer Protocols Handbooks series, chapters include the kind of detailed descriptions and tips necessary for successful results in practice. Authoritative and practical, Peptide and Protein Engineering: From Concepts to Biotechnological Applications will be of great use to scientists in academia and industry seeking a better understanding of the emerging principles and methodologies in peptide and protein engineering.