Gtpase Protocols

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GTPase Protocols

Ed Manser,Thomas Leung
GTPase Protocols Book PDF

Author : Ed Manser,Thomas Leung
Publisher : Springer Science & Business Media
Page : 259 pages
File Size : 49,5 Mb
Release : 2008-02-03
Category : Science
ISBN : 9781592592814

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GTPase Protocols by Ed Manser,Thomas Leung Pdf

In the last 10 years researchers have firmly established key roles for R- related GTPases in almost every aspect of cell biology. In the 1980s the pro- oncogene Ras itself was the focus of interest, though in the 1990s this shifted to the increasing variety of Ras-related proteins. In this new decade much yet needs to be done to establish the role for all the small GTPases now uncovered by the human genome project. In particular, these GTPases need to be und- stood in the appropriate biochemical and cellular contexts. In the process of trying to uncover the role of these versatile proteins, a variety of novel te- niques and methodologies has been developed. These now enable investi- tors to move easily within a diversity of fields ranging from structural studies to real-time in vivo analysis of a GTPase. In recognition of the need for access to key background methodologies, GTPase Protocols: The Ras Superfamily is devoted to techniques that are pr- ently widely used and that will continue to be the standard for researchers worldwide. Each chapter is aimed at supplying detailed methodologies to allow reproduction in any laboratory, while also providing the general pr- ciples on which the methods are based. Some of the techniques grouped in the first section apply broadly to small GTPases, whereas others in Part II are more applicable within each GTPase subfamily.

GTPase Protocols

Edward J. Manser
GTPase Protocols Book PDF

Author : Edward J. Manser
Publisher : Unknown
Page : 280 pages
File Size : 49,6 Mb
Release : 2002
Category : Guanosine triphosphatase
ISBN : OCLC:990747391

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GTPase Protocols by Edward J. Manser Pdf

Small GTPase binding proteins (GTPases) are an ancient group of proteins that play key roles in almost every aspect of cell biology, from cell proliferation to nuclear transport. In GTPase Protocols: The Ras Superfamily, Edward J. Manser and Thomas Leung have collected the key techniques currently in use to probe the function of these ubiquitous proteins both in vitro and in vivo. Presented in a format that ensures ready reproducibility by accomplished experimentalists who have refined the various methods in their laboratories, each technique includes step-by-step instructions, tips on avoiding pitfalls and troubleshooting, and ancillary notes explaining how to adapt each procedure in the event of problems. The methods cover the spectrum of core techniques required for the five major GTPase subfamilies (Ras, Rho, Rab, Arf, and Ran) and permit a diversity of applications ranging from structural studies on a GTPase to real time in vivo analysis. Timely and highly practical, GTPase Protocols: The Ras Superfamily illuminates the powerful techniques used by investigators today to study this special family of proteins that plays such important roles in human health and disease.

Signal Transduction Protocols

David A. Kendall,Stephen J. Hill
Signal Transduction Protocols Book PDF

Author : David A. Kendall,Stephen J. Hill
Publisher : Springer Science & Business Media
Page : 305 pages
File Size : 43,7 Mb
Release : 2008-02-02
Category : Science
ISBN : 9781592595280

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Signal Transduction Protocols by David A. Kendall,Stephen J. Hill Pdf

As our understanding of the biological sciences expands, the bou- aries between traditional disciplines tend to blur at the edges. Physio- gists and pharmacologists, for instance, now need to embrace techniques that until recently were the strict preserves of biochemists and mole- lar biologists. However, the acquisition of new technologies can be a time-consuming and frustrating business, and unless an expert is on hand to give instruction, precious hours can be spent poring over half-described Methods sections with no guarantee of eventual success. The aim of Signal Transduction Protocols has been to get experts with "hands-on" experience in particular techniques to give detailed accounts of experimental protocols in a recipe-type format, which we hope will circumvent the problems of ambiguity often encountered when reading the literature. The techniques described in Signal Transduction Protocols are those that we think will be most useful in addressing questions in the area of receptor-mediated cell signaling, with particular regard to those receptors that are part of the G-protein-linked superfamily. To keep it to a manageable size, we have omitted any reference to electrophysi- ogy and have instead concentrated on more biochemical approaches.

Receptor Signal Transduction Protocols

Gary B. Willars,R. A. John Challiss
Receptor Signal Transduction Protocols Book PDF

Author : Gary B. Willars,R. A. John Challiss
Publisher : Springer Science & Business Media
Page : 420 pages
File Size : 51,6 Mb
Release : 2008-02-03
Category : Science
ISBN : 9781592597543

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Receptor Signal Transduction Protocols by Gary B. Willars,R. A. John Challiss Pdf

This second edition of Receptor Signal Transduction Protocols not only has a new editor, but also a greater focus on G-protein-coupled receptors, their properties per se, and their coupling to immediate downstream binding partners—principally, although not exclusively, the heterotrimeric G-proteins. The new edition combines updates of key chapters from the first edition, as well as a large number of new contributions covering key methodologies that have emerged, or been extended to receptor/G-protein research, in the past 5–6 years. In common with many fields, the range of methods used to assess the first steps in signal transduction are continually expanding and methods that might have been considered too specialized five years ago are now sufficiently routine to be included here. Unlike many research areas, where off-the-shelf kits have made research basically foolproof, signal transduction research still requires considerable expertise, and the methods included here are provided by internationally recognized experts in their fields who have many years of experience using the methods they describe. This not only allows each chapter to impart a clear description of the method, but also to furnish invaluable troubleshooting advice for when things do not go entirely according to plan. Once again we would like to thank the Series Editor, John Walker, for the invitation to compile this second edition, and to express our gratitude to all of the authors who have enthusiastically agreed to provide the uniformly excellent contributions.

Rab GTPases

Guangpu Li (Molecular biologist)
Rab GTPases Book PDF

Author : Guangpu Li (Molecular biologist)
Publisher : Unknown
Page : 358 pages
File Size : 43,5 Mb
Release : 2015
Category : Biochemistry
ISBN : 1493925695

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Rab GTPases by Guangpu Li (Molecular biologist) Pdf

This volume covers the latest technological advances in the characterization of the biosynthesis and functions of Rab GTPases and their regulation by guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs). The book consists of 28 chapters, and starts with an overview of the Rab GTPase family. The next few chapters describe systematic approaches to the identification and classification of Rabs and Rab GAPs, as well as the detection of Rab isoprenylation and membrane distribution. The last few chapters examine the biochemical and functional properties of individual Rabs in the order of exocytic, recycling, and endocytic Rabs. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Instructive and practical, Rab GTPases: Methods and Protocols approaches each topic with great detail and is a valuable resource for researchers and students interested in the field of Rab GTPases.

Neutrophil Methods and Protocols

Mark T. Quinn,Frank R. DeLeo,Gary M. Bokoch
Neutrophil Methods and Protocols Book PDF

Author : Mark T. Quinn,Frank R. DeLeo,Gary M. Bokoch
Publisher : Springer Science & Business Media
Page : 1099 pages
File Size : 51,8 Mb
Release : 2007-08-02
Category : Medical
ISBN : 9781588297884

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Neutrophil Methods and Protocols by Mark T. Quinn,Frank R. DeLeo,Gary M. Bokoch Pdf

This book provides a concise set of protocols for assessing basic neutrophil functions, investigating specialized areas in neutrophil research, and completing step-by-step diagnostic assays of common neutrophil disorders. Each of the protocols is written by leading researchers in the field and includes hints for success, as well as guidance for troubleshooting. Scientists and clinicians will find this collection an invaluable aid.

PCR Cloning Protocols

Bing-Yuan Chen,Harry W. Janes
PCR Cloning Protocols Book PDF

Author : Bing-Yuan Chen,Harry W. Janes
Publisher : Springer Science & Business Media
Page : 429 pages
File Size : 45,5 Mb
Release : 2008-02-05
Category : Science
ISBN : 9781592591770

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PCR Cloning Protocols by Bing-Yuan Chen,Harry W. Janes Pdf

PCR Cloning Protocols, Second Edition, updates and expands Bruce White's best-selling PCR Cloning Protocols (1997) with the newest procedures for DNA cloning and mutagenesis. Here the researcher will find readily reproducible methods for all the major aspects of PCR use, including PCR optimization, computer programs for PCR primer design and analysis, and novel variations for cloning genes of special characteristics or origin, with emphasis on long distance PCR and GC-rich template amplification. Also included are both conventional and novel enzyme-free and restriction site-free procedures to clone PCR products into a range of vectors, as well as state-of-the-art protocols to facilitate DNA mutagenesis and recombination, and to clone the challenging uncharacterized DNA flanking a known DNA fragment.

Epithelial Cell Culture Protocols

Clare Wise
Epithelial Cell Culture Protocols Book PDF

Author : Clare Wise
Publisher : Springer Science & Business Media
Page : 407 pages
File Size : 48,5 Mb
Release : 2008-02-05
Category : Medical
ISBN : 9781592591855

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Epithelial Cell Culture Protocols by Clare Wise Pdf

There have been significant advances in research involving the isolation and culture of epithelial cells in the past decade, and many new techniques have been developed. Monolayer cultures can be used to evaluate the nature and behavior of cells, while the use of epithelial cells in model systems has allowed a deeper understanding of cellular and molecular mechanisms and interactions. The aim of this book is to provide a comprehensive, step-by-step guide to many techniques for epithelial cell culture, combining in one volume the more commonly used protocols along with many that are more speci- ized. Epithelial Cell Culture Protocols should help those who are new to this field and want to learn the basic culture techniques, as well as those needing to use more wide ranging and specific protocols. It should be a useful resource on its own, and also complement the other volumes that have been written about cell culture in the Methods in Molecular Biology series. Epithelial Cell Culture Protocols covers a wide variety of protocols, mostly aimed at the researcher, but also a few aimed at clinicians. The est- lishment and maintenance of primary cultures derived from many different tissues and different species is covered. Particular emphasis has been placed on protocols needed to further analyze and assess epithelial cells, for example, by looking at apoptosis and integrins and by measuring membrane capa- tance and confluence. Using different co-culture techniques, it is possible also to develop models to investigate many different systems in vitro.

DNA Methylation Protocols

Ken I. Mills,Bernie H. Ramsahoye
DNA Methylation Protocols Book PDF

Author : Ken I. Mills,Bernie H. Ramsahoye
Publisher : Springer Science & Business Media
Page : 191 pages
File Size : 42,5 Mb
Release : 2008-02-05
Category : Science
ISBN : 9781592591824

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DNA Methylation Protocols by Ken I. Mills,Bernie H. Ramsahoye Pdf

DNA Methylation Protocols offer a set of readily reproducible protocols of the analysis of DNA methylation and methylases. These powerful methods provide the tools necessary for studying methylation at both the global level and the level of sequence, and include many techniques for identifying genes that might be aberrantly methylated in cancer and aging. Additional methods cover genome-wide analysis of abnormal DNA methylation and the isolation and measurement of demethylases and related proteins.

Calcium-Binding Protein Protocols

Hans J. Vogel
Calcium Binding Protein Protocols Book PDF

Author : Hans J. Vogel
Publisher : Springer Science & Business Media
Page : 415 pages
File Size : 51,8 Mb
Release : 2008-02-05
Category : Science
ISBN : 9781592591848

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Calcium-Binding Protein Protocols by Hans J. Vogel Pdf

Calcium plays an important role in a wide variety of biological processes. This divalent metal ion can bind to a large number of proteins; by doing so it modifies their biological activity or their stability. Because of its distinct che- cal properties calcium is uniquely suited to act as an on–off switch or as a light dimmer of biological activities. The two books entitled Calcium-Binding Protein Protocols (Volumes I and II) focus on modern experimental analyses and methodologies for the study of calcium-binding proteins. Both extracel- lar and intracellular calcium-binding proteins are discussed in detail. H- ever, proteins involved in calcium handling (e. g. , calcium pumps and calcium channels), fall outside of the scope of these two volumes. Also, calcium-bi- ing proteins involved in bone deposition will not be discussed, as this specific topic has been addressed previously. The focus of these two books is on studies of the calcium-binding proteins and their behavior in vitro and in vivo. The primary emphasis is on protein chemistry and biophysical methods. Many of the methods described will also be applicable to proteins that do not bind calcium. Calcium-Binding Protein Protocols is divided into three main sections. The section entitled Introduction and Reviews provides information on the role of calcium in intracellular secondary messenger activation mechanisms. Mo- over, unique aspects of calcium chemistry and the utilization of calcium in dairy proteins, as well as calcium-binding proteins involved in blood clotting, are addressed.

RT-PCR Protocols

Nicola King,Joe O’Connell
RT PCR Protocols Book PDF

Author : Nicola King,Joe O’Connell
Publisher : Springer Science & Business Media
Page : 370 pages
File Size : 53,5 Mb
Release : 2008-02-04
Category : Science
ISBN : 9781592592838

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RT-PCR Protocols by Nicola King,Joe O’Connell Pdf

Until the mid 1980s, the detection and quantification of a specific mRNA was a difficult task, usually only undertaken by a skilled molecular biologist. With the advent of PCR, it became possible to amplify specific mRNA, after first converting the mRNA to cDNA via reverse transcriptase. The arrival of this technique—termed reverse transcription-PCR (RT-PCR)—meant that mRNA suddenly became amenable to rapid and sensitive analysis, without the need for advanced training in molecular biology. This new accessibility of mRNA, which has been facilitated by the rapid accumulation of sequence data for human mRNAs, means that every biomedical researcher can now include measurement of specific mRNA expression as a routine component of his/her research plans. In view of the ubiquity of the use of standard RT-PCR, the main objective of RT-PCR Protocols is essentially to provide novel, useful applications of RT-PCR. These include some useful adaptations and applications that could be relevant to the wider research community who are already familiar with the basic RT-PCR protocol. For example, a variety of different adaptations are described that have been employed to obtain quantitative data from RT-PCR. Quantitative RT-PCR provides the ability to accurately measure changes/imb- ances in specific mRNA expression between normal and diseased tissues.

Peptide Research Protocols

Janet J. Maguire,Anthony P. Davenport
Peptide Research Protocols Book PDF

Author : Janet J. Maguire,Anthony P. Davenport
Publisher : Springer Science & Business Media
Page : 241 pages
File Size : 50,8 Mb
Release : 2008-02-04
Category : Medical
ISBN : 9781592592890

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Peptide Research Protocols by Janet J. Maguire,Anthony P. Davenport Pdf

A panel of multidisciplinary experts describes in detail readily reproducible methods to investigate all aspects of the endothelin system from its synthesis and metabolism, to its function in health and disease. Theses methods use state-of-the-art molecular techniques to quantify the expression of mRNA for both endothelin receptors and the endothelin converting enzymes. They show how peptides, precursors, receptors, and synthetic enzymes can be localized and quantified in plasma, culture supernatants, tissue homogenate, and tissue sections using antibodies. Several in vivo protocols illustrate the role of the endothelin peptides in healthy human individuals and describe animal models that can be used to predict the therapeutic potential of cardiovascular drugs that manipulate endothelin synthesis or function.

PCR Mutation Detection Protocols

Bimal D. M. Theophilus,Ralph Rapley
PCR Mutation Detection Protocols Book PDF

Author : Bimal D. M. Theophilus,Ralph Rapley
Publisher : Springer Science & Business Media
Page : 214 pages
File Size : 46,7 Mb
Release : 2008-02-02
Category : Science
ISBN : 9781592592739

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PCR Mutation Detection Protocols by Bimal D. M. Theophilus,Ralph Rapley Pdf

1Bimal D. Theophilus and Ralph Rapley provide biological and clinical investigators with a comprehensive collection of new, recent, and updated PCR-based screening methods suitable for detecting the presence of both known and novel mutations. The methods cover point mutations (e.g., ASO-PCR, SSCP, DGGE, chemical cleavage), deletions (multiplex PCR, FISH, blotting), non-sense mutations (PTT), and more. The new and exciting techniques of DNA array analysis, along with such recently developed experimental methods as conformation-sensitive gel electrophoresis, are also included. Each chapter explains the basic theory behind the technique and provides valuable notes essential for its successful execution.

Liposome Methods and Protocols

Subhash C. Basu,Manju Basu
Liposome Methods and Protocols Book PDF

Author : Subhash C. Basu,Manju Basu
Publisher : Springer Science & Business Media
Page : 250 pages
File Size : 49,8 Mb
Release : 2008-02-04
Category : Medical
ISBN : 9781592591756

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Liposome Methods and Protocols by Subhash C. Basu,Manju Basu Pdf

In vitro utilization of liposomes is now recognized as a powerful tool in many bioscience investigations and their associated clinical studies, e.g., liposomes in drug targeting; liposomes in gene transport across plasma and nuclear membranes; liposomes in enzyme therapy in patients with genetic disorders. However, before these areas can be effectively explored, many basic areas in liposome research require elucidation, including: (a) attachment of liposomes to cell surfaces; (b) permeation of liposomes through the plasma membranes; and (c) stability of liposomes in cell or nuclear matrices. None of these areas have been exhaustively explored and liposome researchers have ample opportunities to contribute to our knowledge. The aim of Liposome Methods and Protocols is to bring together a wide range of detailed laboratory protocols covering different aspects of liposome biology in order to assist researchers in those rapidly advancing medical fields mentioned earlier. With this goal in mind, in each protocol chapter we have detailed the materials to be used, followed by a step-by-step protocol. The Notes section of each protocol is also certain to prove particularly useful, since the authors include troubleshooting tips straight from their benchtops, valuable information that is seldom given in restricted methods sections of standard research journals. For this reason we feel that the book will prove especially useful for all researchers in the liposome field.

E. coli Gene Expression Protocols

Peter E. Vaillancourt
E coli Gene Expression Protocols Book PDF

Author : Peter E. Vaillancourt
Publisher : Springer Science & Business Media
Page : 347 pages
File Size : 45,7 Mb
Release : 2008-02-02
Category : Science
ISBN : 9781592593019

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E. coli Gene Expression Protocols by Peter E. Vaillancourt Pdf

Peter E. Vaillancourt presents a collection of popular and emerging methodologies that take advantage of E. coli's ability to quickly and inexpensively express recombinant proteins. The authors focus on two areas of interest: the use of E. coli vectors and strains for production of pure, functional protein, and the use of E. coli as host for the functional screening of large collections of proteins and peptides. Among the cutting-edge techniques demonstrated are those for rapid high-level expression and purification of soluble and functional recombinant protein and those essential to functional genomics, proteomics, and protein engineering.