Heterogeneity Of T Cells In Atherosclerosis Defined By Single Cell Rna Sequencing And Cytometry By Time Of Flight

Author : Holger Winkels,Dennis Wolf
Publisher : Unknown
Page : 128 pages
File Size : 48,8 Mb
Release : 2021
Category : Electronic
ISBN : OCLC:1256562773

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Heterogeneity of T Cells in Atherosclerosis Defined by Single-cell RNA-sequencing and Cytometry by Time of Flight by Holger Winkels,Dennis Wolf Pdf

Abstract: The infiltration and accumulation of pro- and anti-inflammatory leukocytes within the intimal layer of the arterial wall is a hallmark of developing and progressing atherosclerosis. While traditionally perceived as macrophage- and foam cell-dominated disease, it is now established that atherosclerosis is a partial autoimmune disease that involves the recognition of peptides from ApoB (apolipoprotein B), the core protein of LDL (low-density lipoprotein) cholesterol particles, by CD4+ T-helper cells and autoantibodies against LDL and ApoB. Autoimmunity in the atherosclerotic plaque has long been understood as a pathogenic T-helper type-1 driven response with proinflammatory cytokine secretion. Recent developments in high-parametric cell immunophenotyping by mass cytometry, single-cell RNA-sequencing, and in tools exploring antigen-specificity have established the existence of several unforeseen layers of T-cell diversity with mixed TH1 and T regulatory cells transcriptional programs and unpredicted fates. These findings suggest that pathogenic ApoB-reactive T cells evolve from atheroprotective and immunosuppressive CD4+ T regulatory cells that lose their protective properties over time. Here, we discuss T-cell heterogeneity in atherosclerosis with a focus on plasticity, antigen-specificity, exhaustion, maturation, tissue residency, and its potential use in clinical prediction

Author : Dipayan Rudra,Paula D. Bos,Akihiko Yoshimura
Publisher : Frontiers Media SA
Page : 246 pages
File Size : 50,6 Mb
Release : 2023-03-01
Category : Medical
ISBN : 9782832515846

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Non-Lymphoid Functions of Regulatory T cells in Health and Disease by Dipayan Rudra,Paula D. Bos,Akihiko Yoshimura Pdf

Author : Zorina S. Galis
Publisher : Academic Press
Page : 528 pages
File Size : 44,9 Mb
Release : 2022-06-11
Category : Science
ISBN : 9780128225479

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The Vasculome by Zorina S. Galis Pdf

The Vasculome: From Many, One introduces the fundamental bases of the “unity in diversity of the Vasculome, from the coming together of various cell lineages during development, to its deceptively simple solution for architectural design: the efficient interplay of a few types of building blocks supporting key similar functions throughout the body and their highly specialized functional local variations. Specific examples are included to illustrate how the Vasculome is integral to the function and malfunction of different organs, such as the brain or the kidney. Each section is preceded by an introductory summary that will give a high level unified view of the key concepts illustrated in the various chapters in that section. Zorina Galis' The Vasculome was named a finalist in the Clinical Medicine category of the American Association of Publishers’ 2023 PROSE Awards. 2023 PROSE Awards - Winner: Finalist: Clinical Medicine: Association of American Publishers Brings together leading experts who present the latest biomedical thinking about the vasculature from the integrative perspective of the Vasculome Challenges traditional real and perceived boundaries within vascular research areas and stimulates new fundamental thinking and medical explorations Creates the bases for translating the integrative Vasculome concept into improved fundamental and clinical assessment and management of local and systemic contributions of the vasculature in health and disease

Author : Nadine Suffee,Wilfried Le Goff,Jianmin Chen
Publisher : Frontiers Media SA
Page : 200 pages
File Size : 52,9 Mb
Release : 2024-03-08
Category : Medical
ISBN : 9782832545928

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Cardiometabolic diseases and inflammatory responses by Nadine Suffee,Wilfried Le Goff,Jianmin Chen Pdf

Cardiometabolic diseases are driven by both metabolic disorders (obesity, insulin resistance, non-alcoholic fatty liver diseases, and atherosclerosis) and chronic inflammation (e.g. in diabetes, hypertension and autoimmune diseases), leading to coronary artery disease and heart failure. The perivascular or pericardiac adipose tissue expansion affecting both the systemic and tissue compartment is favored in cardiometabolic disease development. This adipose tissue is a major component of the cardiovascular system that is dysregulated during the consumption of fat-enriched diets. Additionally, fat-enriched diets profoundly impact the response of immune cells in specialized tissues, as well as the activation and differentiation of tissue-resident progenitors. This results in both dysfunction and remodeling that is not limited to tissues, but also to cell activity. The fate of cardiovascular diseases associated with metabolic disorders promotes the imbalance in pro- and anti-inflammatory environments.

Author : Rhian M. Touyz,Christian Delles
Publisher : Springer
Page : 503 pages
File Size : 55,6 Mb
Release : 2019-08-02
Category : Medical
ISBN : 9783030164812

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Textbook of Vascular Medicine by Rhian M. Touyz,Christian Delles Pdf

This textbook focuses on the vascular biology and physiology that underlie vascular disorders in clinical medicine. Vascular biomedicine is a rapidly growing field as new molecular mechanisms of vascular health and disease are unraveled. Many of the major cardiovascular diseases including coronary artery disease, heart failure, stroke and vascular dementia are diseases of the vasculature. In addition vascular injury underpins conditions like kidney failure and cardiovascular complications of diabetes. This field is truly multidisciplinary involving scientists in many domains such as molecular and vascular biology, cardiovascular physiology and pharmacology and immunology and inflammation. Clinically, specialists across multiple disciplines are involved in the management of patients with vascular disorders, including cardiologists, nephrologists, endocrinologists, neurologists and vascular surgeons. This book covers a wide range of topics and provides an overview of the discipline of vascular biomedicine without aiming at in-depth reviews, but rather offering up-to-date knowledge organized in concise and structured chapters, with key points and pertinent references. The structure of the content provides an integrative and translational approach from basic science (e.g. stem cells) to clinical medicine (e.g. cardiovascular disease). The content of this book is targeted to those who are new in the field of vascular biology and vascular medicine and is ideal for medical students, graduate and postgraduate students, clinical fellows and academic clinicians with an interest in the vascular biology and physiology of cardiovascular disease and related pathologies.

Author : Anonim
Publisher : Frontiers Media SA
Page : 150 pages
File Size : 46,9 Mb
Release : 2024-05-14
Category : Medical
ISBN : 9782832549001

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Single-Cell OMICs Analyses in Cardiovascular Diseases by Anonim Pdf

Single-cell OMICs analyses have recently become one of the most promising tools to probe biology at the cellular level, in large part due to its ability to address issues beyond the bulk analysis – a window into cellular heterogeneity. The ability to profile transcriptomic, epigenomic, proteomics, and metabolomics at the single cell level including more recently the spatial information has enhanced our ability to understand interactions between biomolecules in different contexts leading to the discovery of specific cellular subpopulations as well as biological mechanisms underlying pathologies which may be amenable to therapeutic interventions. The scale and availability of a variety of technologies to measure intricate molecular details have provided an impetus to research in many disease areas, including cardiovascular medicine.

Author : Dennis Wolf,Holger Winkels,Norbert Gerdes,Florian Kahles,Partha Dutta,Chiara Giannarelli
Publisher : Frontiers Media SA
Page : 234 pages
File Size : 50,7 Mb
Release : 2023-02-08
Category : Medical
ISBN : 9782832514030

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Immune and Autoimmune Mechanisms in Cardiovascular Disease by Dennis Wolf,Holger Winkels,Norbert Gerdes,Florian Kahles,Partha Dutta,Chiara Giannarelli Pdf

Author : Tamás Röszer
Publisher : Springer Nature
Page : 224 pages
File Size : 51,5 Mb
Release : 2020-08-03
Category : Medical
ISBN : 9783030504809

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The M2 Macrophage by Tamás Röszer Pdf

Macrophages are core components of the innate immune system. Once activated, they may have either pro- or anti-inflammatory effects that include pathogen killing, safe disposal of apoptotic cells or tissue renewal. The activation state of macrophages is conceptualized by the so-called M1/M2 model of polarization. M2 macrophages are not simply antagonists of M1 macrophages; rather, they represent a network of tissue resident macrophages with roles in tissue development and organ homeostasis. M2 macrophages govern functions at the interfaces of immunity, tissue development and turnover, metabolism, and endocrine signaling. Dysfunction in M2 macrophages can ruin the healthy interplay between the immune system and metabolic processes, and lead to diseases such as insulin resistance, metabolic syndrome, and type 1 and 2 diabetes mellitus. Furthermore, M2 macrophages are essential for healthy tissue development and immunological self-tolerance. Worryingly, these functions of M2 macrophages can also be disrupted, resulting in tumor growth and autoimmunity. This book comprehensively discusses the biology of M2 macrophages, summarizes the current state of knowledge, and highlights key questions that remain unanswered.

Author : Shailendra K. Saxena,Hridayesh Prakash
Publisher : BoD – Books on Demand
Page : 416 pages
File Size : 43,9 Mb
Release : 2021-08-25
Category : Medical
ISBN : 9781838807658

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Innate Immunity in Health and Disease by Shailendra K. Saxena,Hridayesh Prakash Pdf

The book focuses on various aspects and properties of innate immunity, whose deep understanding is integral for safeguarding the human race from further loss of resources and economies due to innate immune response-mediated diseases. Throughout this book, we examine the individual mechanisms by which the innate immune response acts to protect the host from pathogenic infectious agents and other non-communicable diseases. Written by experts in the field, the volume discusses the significance of macrophages in infectious disease, tumor metabolism, and muscular disorders. Chapters cover such topics as the fate of differentiated macrophages and the molecular pathways that are important for the pathologic role of macrophages.

Author : Henry M. Greenberg,Edward M. Dwyer
Publisher : Unknown
Page : 500 pages
File Size : 46,9 Mb
Release : 1982
Category : Medical
ISBN : STANFORD:36105037929069

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Sudden Coronary Death by Henry M. Greenberg,Edward M. Dwyer Pdf

Author : Peter W.F Wilson
Publisher : Springer Science & Business Media
Page : 273 pages
File Size : 47,5 Mb
Release : 2013-06-29
Category : Medical
ISBN : 9781475793109

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Atlas of Atherosclerosis by Peter W.F Wilson Pdf

This new edition is an integral source of information covering topics such as newer coronary risk factors, high-density lipoprotein metabolism, lipid-lowering drugs, endothelium and thrombosis in atherogenesis, and contributing risk factors. With over 500 exceptional photographs, diagrams, and charts, each chapter illustrates an important facet of diagnosing and treating this common and often fatal disease.

Author : Paul A. Schulte,Frederica P. Perera
Publisher : Academic Press
Page : 588 pages
File Size : 47,7 Mb
Release : 2012-12-02
Category : Science
ISBN : 9780323138574

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Molecular Epidemiology by Paul A. Schulte,Frederica P. Perera Pdf

This book will serve as a primer for both laboratory and field scientists who are shaping the emerging field of molecular epidemiology. Molecular epidemiology utilizes the same paradigm as traditional epidemiology but uses biological markers to identify exposure, disease or susceptibility. Schulte and Perera present the epidemiologic methods pertinent to biological markers. The book is also designed to enumerate the considerations necessary for valid field research and provide a resource on the salient and subtle features of biological indicators.

Author : Andreas Radbruch
Publisher : Springer Science & Business Media
Page : 365 pages
File Size : 47,7 Mb
Release : 2013-03-14
Category : Science
ISBN : 9783662041291

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Flow Cytometry and Cell Sorting by Andreas Radbruch Pdf

The analysis and sorting of large numbers of cells with a fluorescence-activated cell sorter (FACS) was first achieved some 30 years ago. Since then, this technology has been rapidly developed and is used today in many laboratories. A Springer Lab Manual Review of the First Edition: "This is a most useful volume which will be a welcome addition for personal use and also for laboratories in a wide range of disciplines. Highly recommended." CYTOBIOS

Author : Mousumi Debnath,Godavarthi B.K.S. Prasad,Prakash S. Bisen
Publisher : Springer Science & Business Media
Page : 520 pages
File Size : 47,5 Mb
Release : 2010-01-29
Category : Medical
ISBN : 9789048132614

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Molecular Diagnostics: Promises and Possibilities by Mousumi Debnath,Godavarthi B.K.S. Prasad,Prakash S. Bisen Pdf

A rapid development in diverse areas of molecular biology and genetic engineering resulted in emergence of variety of tools. These tools are not only applicable to basic researches being carried out world over, but also exploited for precise detection of abnormal conditions in plants, animals and human body. Although a basic researcher is well versed with few techniques used by him/her in the laboratory, they may not be well acquainted with methodologies, which can be used to work out some of their own research problems. The picture is more blurred when the molecular diagnostic tools are to be used by physicians, scientists and technicians working in diagnostic laboratories in hospitals, industry and academic institutions. Since many of them are not trained in basics of these methods, they come across several gray areas in understanding of these tools. The accurate application of molecular diagnostic tools demands in depth understanding of the methodology for precise detection of the abnormal condition of living body. To meet the requirements of a good book on molecular diagnostics of students, physicians, scientists working in agricultural, veterinary, medical and pharmaceutical sciences, it needs to expose the reader lucidly to: Give basic science behind commonly used tools in diagnostics Expose the readers to detailed applications of these tools and Make them aware the availability of such diagnostic tools The book will attract additional audience of pathologists, medical microbiologists, pharmaceutical sciences, agricultural scientists and veterinary doctors if the following topics are incorporated at appropriate places in Unit II or separately as a part of Unit-III in the book. Molecular diagnosis of diseases in agricultural crops Molecular diagnosis of veterinary diseases. Molecular epidemiology, which helps to differentiate various epidemic strains and sources of disease outbreaks. Even in different units of the same hospital, the infections could be by different strains of the same species and the information becomes valuable for infection control strategies. Drug resistance is a growing problem for bacterial, fungal and parasitic microbes and the molecular biology tools can help to detect the drug resistance genes without the cultivation and in vitro sensitivity testing. Molecular diagnostics offers faster help in the selection of the proper antibiotic for the treatment of tuberculosis, which is a major problem of the in the developing world. The conventional culture and drug sensitivity testing of tuberculosis bacilli is laborious and time consuming, whereas molecular diagnosis offers rapid drug resistant gene detection even from direct clinical samples. The same approach for HIV, malaria and many more diseases needs to be considered. Molecular diagnostics in the detection of diseases during foetal life is an upcoming area in the foetal medicine in case of genetic abnormalities and infectious like TORCH complex etc. The book will be equally useful to students, scientists and professionals working in the field of molecular diagnostics.

Author : J. Philip McCoy, Jr
Publisher : Humana
Page : 356 pages
File Size : 43,5 Mb
Release : 2020-09-15
Category : Medical
ISBN : 1493996525

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Immunophenotyping by J. Philip McCoy, Jr Pdf

This volume presents the latest collection of immunophenotypic techniques and applications used in research and clinical settings. Chapters in this book cover topics such as constructions of high dimensions fluorescence and mass cytometry panels; fluorescence barcoding; using dried or lyophilized reagents; and immunophenotypic examples of specific cell types. The book concludes with a discussion on the critical roles of quality control and immunophenotyping in the clinical environment. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Immunophenotyping: Methods and Protocols is a valuable resource for any researchers, clinician, or scientist interested in learning more about this evolving field.