Histochemistry

Author : Carlo Pellicciari,Marco Biggiogera,Manuela Malatesta
Publisher : Springer Nature
Page : 351 pages
File Size : 53,8 Mb
Release : 2022-09-24
Category : Science
ISBN : 9781071626757

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Histochemistry of Single Molecules by Carlo Pellicciari,Marco Biggiogera,Manuela Malatesta Pdf

This volume details histochemical techniques for the detection of specific molecules or metabolic processes, both at light and electron microscopy. Chapters are divided into seven sections covering Vital histochemistry, Carbohydrate histochemistry, Protein histochemistry, Lipid histochemistry, Nuclear histochemistry, Plant histochemistry and Histochemistry for Nanoscience. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. The volume also contains three discursive chapters on Histochemistry in advanced cytometry, Lectins and Detection of molecules in plant cell walls by fluorescence microscopy. Authoritative and cutting-edge, Histochemistry of Single Molecules: Methods and Protocols, Second Edition aims to be a useful practical guide for researchers to help further their study in this field.

Author : Richard W. Horobin
Publisher : Elsevier
Page : 324 pages
File Size : 53,7 Mb
Release : 2014-05-16
Category : Science
ISBN : 9781483164687

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Histochemistry by Richard W. Horobin Pdf

Histochemistry: An Explanatory Outline of Histochemistry and Biophysical Staining describes the histochemical staining of cells and tissues as a major tool applied in biological and medical investigations, both in basic research and in practical applications such as clinical diagnosis. The book may be considered as a guide to understanding the scientific basis of staining procedures and alternate actions to take when common methods do not proceed as expected. The first chapter gives general theoretical ideas from which most part of the book is largely organized around. As the book considers the arts and crafts making up the practice of histochemistry and biological staining, emphasis is given to the common physicochemical aspects of the technically diverse methodologies involved. Hence, the author has drawn ideas and information from physicochemically and biochemically related fields, such as chromatography, pharmacology, photography, tanning, and textile dyeing. The bulk of the book is structured around groups of practical procedures, such as fixation, staining with dyestuffs, metal impregnation, and selective extraction as a test of significance. Lastly, general essays on the usefulness of staining theories and on the problems arising from reagent impurities are given as examples. The text is suitable for students and researchers in the fields of physiology, biochemistry, and pharmacology. Clinical laboratory scientists and those involved in the life sciences and biotechnology will benefit from reading the book.

Author : John Alan Kiernan
Publisher : Pergamon
Page : 454 pages
File Size : 44,9 Mb
Release : 1990
Category : Medical
ISBN : UOM:39015046946524

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Histological & Histochemical Methods by John Alan Kiernan Pdf

Revised and updated edition (1st was 1981) of a textbook on chemical and physical principles of fixation, staining and histochemistry. For students i all biological subjects using histological techniques, as well as researcher and medical laboratory technologists. Annotation copyright Book News, Inc

Author : P. J. Stoward
Publisher : Springer
Page : 201 pages
File Size : 44,6 Mb
Release : 2013-11-11
Category : Science
ISBN : 9781489932600

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Fixation in Histochemistry by P. J. Stoward Pdf

by by Professor Professor A. A. G. G. Everson Pearse Pearse The tide of of the Symposium, at at which the the papers which compromise this volume were presented, is ofthe utmost importance. It was not 'Fixation and Tissue Destruction' or 'Fixation 'Fixation and and Loss Loss ofTissue Components', Components', but but 'Fixation and and Tissue Tissue Preservation'. Historical Historical fixatives, fixatives, some some still still with with us us in the field oflight oflight microscopy microscopy after after over over 100 100 years, are are not not less less guilty guilty than than the the new new historical 'fixatives' of of ultrastructural ultrastructural practice practice some some ofwhich of which remove up up to to 60% 60% ofthe of the material material originally present in in the specimen and and convert the remainder into chemically inert products. There There must must be be few few histochemists histochemists who who do not not appreciate appreciate the great need need for for improve improve ments ments in in the the practice practice of of fixation, fixation, but but whence whence can can we we hopefully hopefully expect expect the arrival arrival of such such improvements? improvements ? Apart Apart from from out out ofthe ofthe blue, or revolutionary, revolutionary, advances advances such such as as the advent advent of of glutaralde hyde, there are perhaps three principal sources.

Author : Z. Lojda,R. Gossrau,T.H. Schiebler
Publisher : Springer Science & Business Media
Page : 350 pages
File Size : 52,5 Mb
Release : 2012-12-06
Category : Medical
ISBN : 9783642672347

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Enzyme Histochemistry by Z. Lojda,R. Gossrau,T.H. Schiebler Pdf

During recent years enzyme histochemical reactions have increasingly been considered as important, the reason being that enzyme histo chemistry is now a well-established link between morphology and bio chemistry. The development of numerous new methods and in particular the improvement of existing techniques contributed to the expansion of enzyme histochemical reactions. Today, the use of these methods allows detailed insight into molecular processes of single cells and their constituents. The selection of a suitable method for enzyme histochemical investigations needs thorough knowledge and critical evaluation of the reactions de scribed for the histochemical demonstration of enzymes and introduced in laboratory practice. Often, it is difficult for scientists primarily concerned with the application of methods and for laboratory assistants to comment on the value of an enzyme histochemical reaction. Our book will serve as a guide in this respect. It contains the most important histochemical methods for the localization of enzymes, all of which were checked by the authors themselves. These methods were often modified and frequently used for numerous different investigations of healthy and diseased organs in basic research and in routine practice.

Author : Jinsong Zhou
Publisher : Walter de Gruyter GmbH & Co KG
Page : 242 pages
File Size : 43,8 Mb
Release : 2017-08-21
Category : Science
ISBN : 9783110531398

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Histochemistry by Jinsong Zhou Pdf

This book systematically illustrates theories and technologies in Histochemistry, including different kinds of enzymes, immunohistochemistry, polymerase chain reaction, related electron microscopic cytochemical techniques as well as the quantitative assay metrology. Abundant experiments as well as vivid images are demonstrated, making the book an essential reference for both graduate students and researchers in biochemistry.

Author : David Evered,Maeve O'Connor
Publisher : John Wiley & Sons
Page : 192 pages
File Size : 44,7 Mb
Release : 2009-09-16
Category : Science
ISBN : 9780470718247

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Trends in Enzyme Histochemistry and Cytochemistry by David Evered,Maeve O'Connor Pdf

The Novartis Foundation Series is a popular collection of the proceedings from Novartis Foundation Symposia, in which groups of leading scientists from a range of topics across biology, chemistry and medicine assembled to present papers and discuss results. The Novartis Foundation, originally known as the Ciba Foundation, is well known to scientists and clinicians around the world.

Author : Hans Lyon
Publisher : Springer Science & Business Media
Page : 582 pages
File Size : 55,6 Mb
Release : 2012-12-06
Category : Medical
ISBN : 9783642737428

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Theory and Strategy in Histochemistry by Hans Lyon Pdf

If you want practical information on how to use this book please refer to "Note to the Readers" p. VII. Histochemistry and cytochemistry are essential tools in biomedical research and routine service laboratories. Most texts on histochemistry fall into one of two categories: 1. Encyclopaedic texts covering all or nearly all information available on the whole or selected parts of histochemistry. 2. Reviews or surveys of methods found to be useful by the author(s). While the former category often appeals to the more philosophically inclined reader, direct guidance on the selection of technique may be difficult to find. In contrast, the latter category are often excellent sources for details on how to perform a particular method with a reasonable chance of success. Consideration of the exact mechanism of staining, of possible reasons for failure, and of alternative techniques are, however, frequently lacking. This book is an introduction to the scientific basis of histochemistry and is intended to provide a background for the selection and development of appro priate methods. It is not a "cook book" and readers expecting exhaustive methodological descriptions will be disappointed. Although most ofthe contributors to this book would not describe themselves as histochemists, they have all at some time found it essential to develop a basic understanding of histochemistry. This book contains the information they would have greatly appreciated ready access to at that time.

Author : Johnson K. Gao
Publisher : CRC Press
Page : 140 pages
File Size : 53,9 Mb
Release : 1993-06-11
Category : Science
ISBN : 0849343232

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Polyethylene Glycol as an Embedment for Microscopy and Histochemistry by Johnson K. Gao Pdf

Polyethylene Glycol as an Embedment for Microscopy and Histochemistry is the first book devoted to discussing polyethylene glycol (PEG) as a biological sample embedment for microscopy (EM and LM). Its contributors are international authorities in all areas of investigation using PEG as an embedding medium. The book provides broad coverage of a variety of methods, including low molecular PEG-cryosection, agarose block section-transferring technique, PEG section for scanning electron microscopy, nucleic acid in situ hybridization study, monoclonal antibody screening, PEG-celloidin mixture embedding method, and diethylene glycol distearate used as removable embedment. This practical volume will interest histologists, histochemists, cytochemists, cell biologists, and other researchers using PEG in microscopy.

Author : Marie-Francoise Chesselet
Publisher : CRC Press
Page : 232 pages
File Size : 48,7 Mb
Release : 1990-07-03
Category : Medical
ISBN : 0849369126

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In Situ Hybridization Histochemistry by Marie-Francoise Chesselet Pdf

The goal of this fascinating new book is to review the diversity of methods available to apply in situ hybridization histochemistry (ISHH) to a variety of experimental questions. This work includes topics such as synthesis and use of nick-translated DNA probes for ISHH, synthesis and use of oligomeric DNA probes for ISHH, and synthesis and use of RNA probes for ISHH. These interesting chapters describe the preparation of different radiolabeled probes for ISHH. They also discuss their respective advantages and limitations, and describe current results based on the use of these various probes. Sections of the text highlight low and high resolution autoradiography for ISHH, the use of biotin-labeled probes for ISHH, as well as the use of ISHH in combination with established anatomical techniques. In Situ Hybridization Histochemistry answers all of your questions regarding the quantification of ISHH. It also provides a practical description of typical protocols, both from molecular biology and histology. Investigators will understand and value this useful, powerful tool-whatever their backgrounds might be.

Author : K. Shyamsundari,K Hanumantha Rao
Publisher : MJP Publisher
Page : 886 pages
File Size : 46,7 Mb
Release : 2019-06-07
Category : Juvenile Nonfiction
ISBN : 8210379456XXX

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Histochemistry in Focus by K. Shyamsundari,K Hanumantha Rao Pdf

Fixatives and Methods of Fixation, Tissue Processing, Theory of Staining, Decalcification, Preparation of Stains, Mountants, Frozen Methods, Carbohydrates, Protein, Amyloids, Nucleic Acids, Lipids, Pigments, Minerals, Microorganisms in Sections, Enzymes, Connective Tissue, Neurological Studies, Endocrine Glands, Microwave Histology, Ultrahistochemistry, Techniques in Cell Biology, Methods for Special Organs, Invertebrate Staining Methods, Mast Cells, Immunocytochemistry.

Author : Anonim
Publisher : Elsevier
Page : 524 pages
File Size : 53,6 Mb
Release : 1971-01-01
Category : Medical
ISBN : 0080861598

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Histochemistry of Nervous Transmission by Anonim Pdf

Histochemistry of Nervous Transmission

Author : Masahiko Mori
Publisher : CRC Press
Page : 307 pages
File Size : 41,6 Mb
Release : 2019-06-04
Category : Medical
ISBN : 9781000005776

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Histochemistry of the Salivary Glands by Masahiko Mori Pdf

First published in 1991, Histochemistry of the Salivary Glands is a well-structured, in depth exploration of Cytoskeletal proteins and their function. With references and data from numerous sources, this book is essential for students of medicine and practitioners in their respective fields.

Author : Robert M. Rosenbaum
Publisher : Springer
Page : 327 pages
File Size : 46,6 Mb
Release : 2013-11-21
Category : Science
ISBN : 9781489927910

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Third International Congress of Histochemistry and Cytochemistry by Robert M. Rosenbaum Pdf

Author : Alan R. Biggs
Publisher : CRC Press
Page : 345 pages
File Size : 46,7 Mb
Release : 2019-06-13
Category : Technology & Engineering
ISBN : 9780429555855

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Cytology, Histology and Histochemistry of Fruit Tree Diseases by Alan R. Biggs Pdf

Published in 1993: This book provides a comprehensive discussion of the relationships between host plant structure and pathological anatomy. Topics include the structure and development of gum ducts, wound reactions, systemic invasion by bacterial pathogens, diseases caused by MLOs, and responses to cold temperatures.