Hplc Of Biological Macromolecules

Author : Karen M. Gooding,Fred E. Regnier
Publisher : CRC Press
Page : 806 pages
File Size : 51,9 Mb
Release : 2002-01-08
Category : Science
ISBN : 0203911598

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Hplc Of Biological Macro- Molecules, Revised And Expanded by Karen M. Gooding,Fred E. Regnier Pdf

Completely revised to reflect the innovations in HPLC from the past decade, this authoritative reference presents practical strategies for the evaluation and analysis of proteins, peptides, and polynucleotides. Offering class-specific applications for the characterization and fractionation of biological macromolecules, the book contains material on organic supports, size exclusion, ion exchange, hydrophobic interaction, and metal interaction chromatography. Leading experts summarize specialized detection systems, provides discussions on the chemical and biological properties of specific biomolecules, include detailed guidelines for the development of analytical techniques, and more.

Author : Karen M. Gooding,Fred E. Regnier
Publisher : Unknown
Page : 704 pages
File Size : 46,5 Mb
Release : 1990
Category : Science
ISBN : UOM:39015017977979

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HPLC of Biological Macromolecules by Karen M. Gooding,Fred E. Regnier Pdf

The technique; Polypeptides; Polynucleotides.

Author : Karen M. Gooding,Fred E. Regnier
Publisher : CRC Press
Page : 0 pages
File Size : 44,5 Mb
Release : 2002-01-08
Category : Science
ISBN : 082470665X

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Hplc Of Biological Macro- Molecules, Revised And Expanded by Karen M. Gooding,Fred E. Regnier Pdf

Completely revised to reflect the innovations in HPLC from the past decade, this authoritative reference presents practical strategies for the evaluation and analysis of proteins, peptides, and polynucleotides. Offering class-specific applications for the characterization and fractionation of biological macromolecules, the book contains material on organic supports, size exclusion, ion exchange, hydrophobic interaction, and metal interaction chromatography. Leading experts summarize specialized detection systems, provides discussions on the chemical and biological properties of specific biomolecules, include detailed guidelines for the development of analytical techniques, and more.

Author : Karen M. Gooding,Fred E. Regnier
Publisher : CRC Press
Page : 792 pages
File Size : 52,7 Mb
Release : 2002-01-08
Category : Science
ISBN : 082470665X

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Hplc Of Biological Macro- Molecules, Revised And Expanded by Karen M. Gooding,Fred E. Regnier Pdf

Completely revised to reflect the innovations in HPLC from the past decade, this authoritative reference presents practical strategies for the evaluation and analysis of proteins, peptides, and polynucleotides. Offering class-specific applications for the characterization and fractionation of biological macromolecules, the book contains material on organic supports, size exclusion, ion exchange, hydrophobic interaction, and metal interaction chromatography. Leading experts summarize specialized detection systems, provides discussions on the chemical and biological properties of specific biomolecules, include detailed guidelines for the development of analytical techniques, and more.

Author : R. W. A. Oliver
Publisher : Oxford University Press, USA
Page : 260 pages
File Size : 40,5 Mb
Release : 1989
Category : Language Arts & Disciplines
ISBN : UOM:39015058985725

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HPLC of Macromolecules by R. W. A. Oliver Pdf

A wide variety of biological macromolecules, especially proteins and nucleic acids, are now the subject of intense biochemical and medical research interest. The initial separation and purification of the macromolecules is of primary importance in any study. This book will enable researchers to use high performance liquid chromatography in their work.

Author : Barry L. Karger,William S. Hancock
Publisher : Elsevier
Page : 664 pages
File Size : 40,9 Mb
Release : 1996
Category : Capillary electrophoresis
ISBN : 0121821714

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High Resolution Separation and Analysis of Biological Macromolecules by Barry L. Karger,William S. Hancock Pdf

The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. More than 260 volumes have been published (all of them still in print) and much of the material is relevant even today--truly an essential publication for researchers in all fields of life sciences. Key Features * Liquid chromatography * Electrophoresis * Mass spectrometry.

Author : Marie-Isabel Aguilar
Publisher : Springer Science & Business Media
Page : 411 pages
File Size : 55,7 Mb
Release : 2008-02-03
Category : Science
ISBN : 9781592597420

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HPLC of Peptides and Proteins by Marie-Isabel Aguilar Pdf

The introduction of high-performance liquid chromatography (HPLC) to the analysis of peptides and proteins some 25 years ago revolutionized the biological sciences by enabling the rapid and sensitive analysis of peptide and protein structure through the exquisite speed, sensitivity, and resolution that can be easily obtained. Today, HPLC in its various modes has become the pivotal technique in the characterization of peptides and proteins and currently plays a critical role in both our understanding of biological processes and in the development of peptide- and protein-based pharmaceuticals. The number of applications of HPLC in peptide and protein purification continues to expand at an extremely rapid rate. Solid-phase peptide synthesis and recombinant DNA techniques have allowed the production of large quantities of peptides and proteins that need to be highly purified. HPLC techniques are also used extensively in the isolation and characterization of novel proteins that will become increasingly important in the postgenomic age. The design of multidimensional purification schemes to achieve high levels of product purity further demonstrates the power of HPLC techniques not only in the characterization of cellular events, but also in the production of pepti- and protein-based therapeutics. HPLC continues to be at the heart of the analytical techniques with which scientists in both academia and in industry must arm themselves to be able to fully characterize the identity, purity, and potency of peptides and proteins.

Author : Hassan Y. Aboul-Enein
Publisher : CRC Press
Page : 482 pages
File Size : 45,9 Mb
Release : 2020-10-15
Category : Science
ISBN : 9781000146257

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Analytical and Preparative Separation Methods of Biomacromolecules by Hassan Y. Aboul-Enein Pdf

Reports up-to-date research developments on purifying and isolation large organic molecules. The text provides information on high-performance liquid chromatography and capillary electrophoresis (CE) as tools for analyzing biomacromolecules and developing new biochemical and medicinal compounds. It applies biochemical separation technology to the study of macromolecules such as proteins, polysaccharides, nucleic acids and more.

Author : Barry L. Karger,William Hancock
Publisher : Academic Press
Page : 604 pages
File Size : 51,9 Mb
Release : 1996-07-18
Category : Medical
ISBN : UVA:X004063750

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High Resolution Separation and Analysis of Biological Macromolecules, Part B: Applications by Barry L. Karger,William Hancock Pdf

The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. More than 260 volumes have been published (all of them still in print) and much of the material is relevant even today--truly an essential publication for researchers in all fields of life sciences. Liquid chromatography Electrophoresis Mass spectrometry

Author : Anonim
Publisher : Elsevier
Page : 717 pages
File Size : 41,5 Mb
Release : 1988-11-01
Category : Science
ISBN : 0080858465

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Separation of Individual Compound Classes by Anonim Pdf

Separation of Individual Compound Classes

Author : A. Fallon,R.F.G. Booth,L.D. Bell
Publisher : Elsevier
Page : 337 pages
File Size : 44,8 Mb
Release : 1987-07-01
Category : Science
ISBN : 0080858864

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Applications of HPLC in Biochemistry by A. Fallon,R.F.G. Booth,L.D. Bell Pdf

This book is intended to familiarize biochemists with HPLC. Theoretical aspects of each mode of chromatography are discussed in chapters 1-9, providing an understanding of the various modes of chromatography which are now possible using commercially available columns, from reversed phase to affinity. Practical aspects and instrumentation are covered in chapter 10. The bulk of the book, which follows, presents examples and applications of each mode of chromatography in current biochemical practice.

Author : Anonim
Publisher : Academic Press
Page : 622 pages
File Size : 52,9 Mb
Release : 1996-07-08
Category : Science
ISBN : 0121821714

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High Resolution Separation and Analysis of Biological Macromolecules, Part A: Fundamentals by Anonim Pdf

The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. More than 260 volumes have been published (all of them still in print) and much of the material is relevant even today--truly an essential publication for researchers in all fields of life sciences. Liquid chromatography Electrophoresis Mass spectrometry

Author : Colin T. Mant,Robert S. Hodges
Publisher : CRC Press
Page : 960 pages
File Size : 48,8 Mb
Release : 2017-11-22
Category : Medical
ISBN : 9781351440752

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High-Performance Liquid Chromatography of Peptides and Proteins by Colin T. Mant,Robert S. Hodges Pdf

This book consists of a series of 82 precise, easy-to-read articles by internationally renowned scientists and emphasizes the practical approach to HPLC with minimal theory, although the underlying principles for peptide and protein separations are clearly expressed. All of the major modes of microbore, ultrafast and analytical HPLC are discussed, including size-exclusion, ion-exchange, reversed-phase, hydrophobic interaction, and affinity and immunoaffinity chromatography. A section on preparative HPLC, including displacement techniques, is also presented. Problem-solving approaches to the separation of various classes of biologically active peptides and proteins are thoroughly explored, while the importance of peptide standards for monitoring column performance and for optimizing separation conditions is emphasized. Several articles focus on the choice of the correct detection method (electrochemical, UV, fluorescence), as well as the need for a proper knowledge of approaches to column and instrument maintenance and trouble-shooting. A section on predictive approaches deals with both computer simulation of peptide separations and peptide structure. The book also includes complementary techniques to HPLC, as well as other useful applications of HPLC. It enables both novice and experienced chromatographers to realize the full potential of this extremely powerful technique, in the process making an important contribution to scientific literature.

Author : Barry L. Karger
Publisher : Unknown
Page : 555 pages
File Size : 41,5 Mb
Release : 2024-06-30
Category : Electronic
ISBN : OCLC:643458846

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High Resolution Separation and Analysis of Biological Macromolecules by Barry L. Karger Pdf

Author : T.S. Amar Anand Rao
Publisher : GRIN Verlag
Page : 95 pages
File Size : 45,9 Mb
Release : 2012-01-17
Category : Medical
ISBN : 9783656103547

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HPLC and FPLC: Troubleshooting and Standardizing Chromatogram Purification Profiles by T.S. Amar Anand Rao Pdf

Scientific Study from the year 2012 in the subject Biology - Micro- and Molecular Biology, Indian Institute of Science, course: Molecular Biology, language: English, abstract: This is a tool to separate compounds according to its hydrophobicity or hydrophilicity. A gradient is given from hydrophilic to hydrophobic range of solvent moving through a solid matrix. Here are some valuable tips collected through experience and handed over so that the reader can find it useful when the chromatograms and the instruments prove so cross with you. The instrumentation and working for both the systems HPLC and FPLC are almost same. HPLC is High Performance/Pressure Liquid Chromatography and FPLC is Fast Protein Liquid Chromtography.