In Situ Hybridization Protocols

Author : Boye Nielsen
Publisher : Humana Press
Page : 0 pages
File Size : 40,8 Mb
Release : 2014-09-13
Category : Medical
ISBN : 1493914588

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In Situ Hybridization Protocols by Boye Nielsen Pdf

In Situ Hybridization Protocols, Fourth Edition contains 21 protocols that utilize the in situ hybridization technology to document or take advantage of the visualization of specific RNA molecules. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, In Situ Hybridization Protocols, Fourth Edition seeks to aid scientists in the further discovery of new RNA species and uncovering of their cellular functions.

Author : Ian A. Darby
Publisher : Taylor & Francis US
Page : 370 pages
File Size : 41,6 Mb
Release : 2000
Category : Medical
ISBN : 0896036863

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In Situ Hybridization Protocols by Ian A. Darby Pdf

Annotation Darby (human biology, RMIT U., Victoria, Australia) is joined by geneticists, molecular biologists, and pathologists from around the world to describe basic and advanced techniques for hybridization, for whole-mount embryo specimens and at the electron microscope level. Coverage includes protocols for detection of DNA fragmentation in apoptosis, localization of genes to particular chromosomes, and the use of DNA and RNA probes to detect expression in cells or tissue sections. For novice and experienced investigators who need proven and readily reproducible methods. Annotation c. Book News, Inc., Portland, OR (booknews.com)

Author : Boye Schnack Nielsen,Julia Jones
Publisher : Unknown
Page : 392 pages
File Size : 45,7 Mb
Release : 2020
Category : Electronic books
ISBN : 1071606239

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In Situ Hybridization Protocols by Boye Schnack Nielsen,Julia Jones Pdf

This fifth edition volume expands on the previous editions with updated discussions on the many new in situ hybridization (ISH) techniques used by researchers today. New developments in probe designs, detection systems, specificity and sensitivity improvements, and multiplexing combinations are explored. Chapters in this book are organized into seven sections and cover general applications; methods for DNA ISH; methods for cultured cells; methods for wholemount and plant material; automated methods for RNA; multiplexing and combined methods; and targeted selective methods and single molecule detections. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, In Situ Hybridization Protocols, Fifth Edition is a valuable resource for both novice and expert scientists interested in learning more about this exciting and advancing field. .

Author : W. Wisden,B. J. Morris
Publisher : Gulf Professional Publishing
Page : 312 pages
File Size : 54,5 Mb
Release : 2002-08-05
Category : Medical
ISBN : 0123668476

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In Situ Hybridization Protocols for the Brain by W. Wisden,B. J. Morris Pdf

This volume of the International Review of Neurobiology was written to assist researchers without any previous experience with in situ hybridization, allowing them to follow the protocols and expect good results. It contains all the information required for newcomers to achieve successful in situ hybridization results, and methods for improving the technique of those already utilizing it. Published since 1959, International Review of Neurobiology is a well-known series appealing to neuroscientists, clinicians, psychologists, physiologists, and pharmacologists. Led by an internationally renowned editorial board, this important serial publishes both eclectic volumes made up of timely reviews and thematic volumes that focus on recent progress in a specific area of neurobiology research. A well-known series appealing to neuroscientists, clinicians, psychologists, physiologists, and pharmacologists Led by an internationally renowned editorial board, this important serial publishes both eclectic volumes made up of timely reviews and thematic volumes that focus on recent progress in a specific area of neurobiology research

Author : Thomas Liehr
Publisher : Springer Science & Business Media
Page : 447 pages
File Size : 48,7 Mb
Release : 2008-11-26
Category : Science
ISBN : 9783540705819

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Fluorescence In Situ Hybridization (FISH) - Application Guide by Thomas Liehr Pdf

This book is a unique source of information on the present state of the exciting field of molecular cytogenetics and how it can be applied in research and diagnostics. The basic techniques of fluorescence in situ hybridization and primed in situ hybridization (PRINS) are outlined, the multiple approaches and probe sets that are now available for these techniques are described, and applications of them are presented in 36 chapters by authors from ten different countries around the world. The book not only provides the reader with basic and background knowledge on the topic, but also gives detailed protocols that show how molecular cytogenetics is currently performed by specialists in this field. The FISH Application Guide initially provides an overview of the (historical) development of molecular cytogenetics, its basic procedures, the equipment required, and probe generation. The book then describes tips and tricks for making different tissues available for molecular cytogenetic studies. These are followed by chapters on various multicolor FISH probe sets, their availability, and their pot- tial for use in combination with other approaches. The possible applications that are shown encompass the characterization of marker chromosomes, cryptic cytogenetic aberrations and epigenetic changes in humans by interphase and metaphase cyto- netics, studies of nuclear architecture, as well as the application of molecular cytogenetics to zoology, botany and microbiology.

Author : Joanna M. Bridger,Keith Morris
Publisher : Humana Press
Page : 0 pages
File Size : 42,5 Mb
Release : 2010-09-09
Category : Science
ISBN : 1607617889

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Fluorescence in situ Hybridization (FISH) by Joanna M. Bridger,Keith Morris Pdf

Fluorescence in situ Hybridization (FISH) belongs to that special category of well-established molecular biology techniques that, since their inception a few decades ago, have succeeded in keeping a prominent position within the constantly expanding list of laboratory pro- dures for biomedical research and clinical diagnostics. The design simplicity and cost-effectiveness of the early FISH protocols, combined with the signifcant acceleration of discoveries in related technical areas such as fuor- cence microscopy, digital imaging, and nucleic acid technology have prompted the div- sifcation of the original technique into an outstanding number of imaginative and useful applications, and thus have not only held back its outmoding but have also promoted its expansion into different areas of basic and applied research in the post-genomic era. The 34 chapters included in this book aim at portraying the vibrant complexity and diversity of the current FISH protocol landscape, providing cutting-edge examples of va- ous applications for genetic and developmental research, cancer research, reproductive medicine, diagnostic and prognostic purposes, microbial ecology, and evolutionary st- ies. The book is divided in four parts: (I) Core Techniques, (II) Technical Advancements and Novel Adaptations, (III) Translational FISH: Applications for Human Genetics and Medicine, and (IV) Protocols for Model Organisms.

Author : Gal Haimovich
Publisher : Springer Nature
Page : 303 pages
File Size : 48,5 Mb
Release : 2024-06-30
Category : Electronic
ISBN : 9781071637661

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Fluorescence In Situ Hybridization (FISH) by Gal Haimovich Pdf

Author : David G. Wilkinson
Publisher : OUP Oxford
Page : 243 pages
File Size : 45,5 Mb
Release : 1998-11-26
Category : Science
ISBN : 9780191565700

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In Situ Hybridization by David G. Wilkinson Pdf

In situ hybridization is used to reveal the location of specific nucleic acids sequences on chromosomes or in tissues. Visualization of the location of genes on chromosomes or of specific mRNAs or viruses in tissues is crucial for understanding the organization, regulation, and function of genes. It is a therefore a core technique in all areas of biomedical research. In Situ Hybridization: A Practical Approach 2/e is the second edition of one of the most successful Practical Approach books, published in 1992. Since the first edition was published, a number of important technical advances have been made. The new edition has been thoroughly updated to contain protocols detailing the major techniques of in situ hybridization currently in use: in situ hybridization to mRNA with oligonucleotide and RNA probes (radiolabelled and hapten labelled); analysis using light and electron microscopes; whole mount in situ hybridization; double detection of RNAs, and RNA plus protein; and fluorescent in situ hybridization to detect chromosomal sequences. The protocols are complemented by advice on strategies for successful results, descriptions of the theoretical basis of in situ hybridization and important new developments in gene expression databases. The procedures described are widely applicable to many systems. The use of in situ hybridization in PCR is covered in a separate volume: Herrington and O'Leary (Eds) PCR 3 - PCR in situ hybridization: A Practical Approach (OUP, 1997). All the authors have extensive practical experience of establishing reliable techniques of in situ hybridization. This book will be useful to all researchers at all levels who use in situ hybridization.

Author : Gerard J. Nuovo
Publisher : Unknown
Page : 497 pages
File Size : 51,6 Mb
Release : 1997
Category : Cytodiagnosis
ISBN : 078170183X

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PCR in Situ Hybridization by Gerard J. Nuovo Pdf

Author : Giselbert Hauptmann
Publisher : Humana
Page : 0 pages
File Size : 53,9 Mb
Release : 2016-10-05
Category : Medical
ISBN : 1493944630

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In Situ Hybridization Methods by Giselbert Hauptmann Pdf

This volume contains a comprehensive compilation of chromogenic and fluorescent RNA in situ hybridization (ISH) technology in many of its various shades, forms, and applications. The book is organized into a number of parts and chapters focusing on the application of ISH methodologies to different animal species as used in Evolutionary Development (EvoDevo) and Biomedical research, and covering new developments in RNA visualization by fluorescent ISH (FISH). The described (F)ISH protocols employ effective strategies for signal enhancement and target amplification allowing for high signal intensities and drastically improved signal-to-noise ratios. Chromogenic and fluorescent ISH, as specified in the various chapters, are most essential for RNA expression profiling, applied to many fields of research including cellular, developmental, and evolutionary biology, neurobiology and neuropathology. Written for the popular Neuromethods series, chapters include the kind of detail and key implementation advice that ensures successful results in the laboratory. Essential and authoritative, In Situ Hybridization Methods provides detailed protocols for newcomers to ISH, and inspires researchers familiar with the technique to seek and find up-to-date methodology for new and specialized applications.

Author : Nuno F. Azevedo,Carina Almeida
Publisher : Unknown
Page : 128 pages
File Size : 46,8 Mb
Release : 2021
Category : Cytology
ISBN : 1071611178

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Fluorescence In-Situ Hybridization (FISH) for Microbial Cells by Nuno F. Azevedo,Carina Almeida Pdf

Author : Julia M. Polak,James O'Donnell McGee
Publisher : Oxford University Press, USA
Page : 264 pages
File Size : 45,9 Mb
Release : 1990
Category : Literary Criticism
ISBN : UOM:39015057806757

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In Situ Hybridization by Julia M. Polak,James O'Donnell McGee Pdf

Molecular biology and genetic engineering techniques have revolutionized our concepts of gene activity in cellular function. In situ hybridization has contributed to this revolution. It is the only technique which gives precise data on the content and distribution of DNA and mRNA in intactcells and tissues. It is now an invaluable test in understanding the pathophysiology of cellular functions. This book covers all aspects of in situ hybridization. It describes practical procedures and protocols, the scientific background, areas of application, and the limitations of the technology. The contributing authors are all experts. Each chapter contains up-to-date references, many colourillustrations, the latest advances, and prospects for the future. This practical guide will be invaluable to: molecular, cell, and developmental biologists; cyto- and molecular geneticists; clinicians, especially pathologists, biologists, and microbiologists; and scientists seriously interested inthe pathophysiology of cell function and its regulation.

Author : Gerard Morel,Annie Cavalier,Lynda Williams
Publisher : CRC Press
Page : 472 pages
File Size : 50,5 Mb
Release : 2001-01-30
Category : Science
ISBN : 9781420042504

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In Situ Hybridization in Electron Microscopy by Gerard Morel,Annie Cavalier,Lynda Williams Pdf

In situ hybridization is a technique that allows for the visualization of specific DNA and RNA sequences in individual cells, and is an especially important method for studying nucleic acids in heterogeneous cell populations. in situ Hybridization in Electron Microscopy reviews the three main methods developed for the ultrastructural visualization

Author : Trude Schwarzacher,John Heslop-Harrison
Publisher : Garland Science
Page : 203 pages
File Size : 40,9 Mb
Release : 2000
Category : In situ hybridization
ISBN : 185996138X

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Practical in Situ Hybridization by Trude Schwarzacher,John Heslop-Harrison Pdf

Practical in situ Hybridization is aimed at those who wish to learn and use efficient and reliable protocols in their work, and at researchers checking the validity and interpretation of published data.; In situ hybridization methods can be used to identi

Author : Joanna M. Bridger,Keith Morris
Publisher : Humana
Page : 451 pages
File Size : 46,6 Mb
Release : 2016-08-23
Category : Science
ISBN : 1493957244

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Fluorescence in situ Hybridization (FISH) by Joanna M. Bridger,Keith Morris Pdf

Fluorescence in situ Hybridization (FISH) belongs to that special category of well-established molecular biology techniques that, since their inception a few decades ago, have succeeded in keeping a prominent position within the constantly expanding list of laboratory pro- dures for biomedical research and clinical diagnostics. The design simplicity and cost-effectiveness of the early FISH protocols, combined with the signifcant acceleration of discoveries in related technical areas such as fuor- cence microscopy, digital imaging, and nucleic acid technology have prompted the div- sifcation of the original technique into an outstanding number of imaginative and useful applications, and thus have not only held back its outmoding but have also promoted its expansion into different areas of basic and applied research in the post-genomic era. The 34 chapters included in this book aim at portraying the vibrant complexity and diversity of the current FISH protocol landscape, providing cutting-edge examples of va- ous applications for genetic and developmental research, cancer research, reproductive medicine, diagnostic and prognostic purposes, microbial ecology, and evolutionary st- ies. The book is divided in four parts: (I) Core Techniques, (II) Technical Advancements and Novel Adaptations, (III) Translational FISH: Applications for Human Genetics and Medicine, and (IV) Protocols for Model Organisms.