Induced Pluripotent Stem Cells And Human Disease

Author : Kursad Turksen
Publisher : Springer Nature
Page : 426 pages
File Size : 48,6 Mb
Release : 2022-07-02
Category : Science
ISBN : 9781071625859

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Induced Pluripotent Stem Cells and Human Disease by Kursad Turksen Pdf

This detailed volume presents a series of protocols that are representative of recent developments and improvements in induced pluripotent stem cells (iPS cells) and corresponding human disease models. Reflecting the latest technology for generating induced pluripotent stem cells (iPS cells) and their initial characterization, the book explores techniques invaluable both for studies of disease-specific cell types and for their potential applications in regenerative medicine. Written for the highly successful Methods in Molecular Biology series, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Induced Pluripotent Stem Cells and Human Disease: Methods and Protocols serves as a vital guide that is valuable for not only experts but also novices in the stem cell field.

Author : Andras Nagy,Kursad Turksen
Publisher : Springer Nature
Page : 829 pages
File Size : 51,8 Mb
Release : 2022-06-22
Category : Science
ISBN : 9781071621196

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Induced Pluripotent Stem (iPS) Cells by Andras Nagy,Kursad Turksen Pdf

This extensive new edition presents protocols reflecting the great strides made in the study of induced pluripotent stem (iPS) cells. The collection explores new and improved methods for the generation, expansion, and maintenance of iPS cells from different tissue types, characterization of their differentiation pathways along different lineages, and their potential utility in tissue repair and regeneration. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and up-to-date, Induced Pluripotent Stem (iPS) Cells: Methods and Protocols, Second Edition aims to arm stem cell biologists, both novice and expert, with invaluable protocols that are currently being used in various laboratories around the world.

Author : Minoru Tomizawa
Publisher : BoD – Books on Demand
Page : 534 pages
File Size : 47,5 Mb
Release : 2016-07-20
Category : Science
ISBN : 9789535124719

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Pluripotent Stem Cells by Minoru Tomizawa Pdf

Pluripotent stem cells have distinct characteristics: self-renewal and the potential to differentiate into various somatic cells. In recent years, substantial advances have been made from basic science to clinical applications. The vast amount knowledge available makes obtaining concise yet sufficient information difficult, hence the purpose of this book. In this book, embryonic stem cells, induced pluripotent stem cells, and mesenchymal stem cells are discussed. The book is divided into five sections: pluripotency, culture methods, toxicology, disease models, and regenerative medicine. The topics covered range from new concepts to current technologies. Readers are expected to gain useful information from expert contributors.

Author : Deepa Bhartiya,Nibedita Lenka
Publisher : BoD – Books on Demand
Page : 642 pages
File Size : 48,7 Mb
Release : 2013-08-28
Category : Science
ISBN : 9789535111924

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Pluripotent Stem Cells by Deepa Bhartiya,Nibedita Lenka Pdf

Stem cells have generated a lot of excitement among the researchers, clinicians and the public alike. Various types of stem cells are being evaluated for their regenerative potential. Marginal benefit resulting by transplanting autologus stem cells (deemed to be absolutely safe) in various clinical conditions has been proposed to be a growth factor effect rather than true regeneration. In contrast, various pre-clinical studies have been undertaken, using differentiated cells from embryonic stem cells or induced pluripotent stem cells have shown promise, functional improvement and no signs of teratoma formation. The scientists are not in a rush to reach the clinic but a handful of clinical studies have shown promise. This book is a collection of studies/reviews, beginning with an introduction to the pluripotent stem cells and covering various aspects like derivation, differentiation, ethics, etc., and hence would provide insight into the recent standing on the pluripotent stem cells biology. The chapters have been categorized into three sections, covering subjects ranging from the generation of pluripotent stem cells and various means of their derivation from embryonic as well as adult tissues, the mechanistic understanding of pluripotency and narrating the potential therapeutic implications of these in vitro generated cells in various diseases, in addition to the associated pros and cons in the same.

Author : Anonim
Publisher : Springer
Page : 490 pages
File Size : 55,8 Mb
Release : 2011-08-07
Category : Electronic
ISBN : 1617792020

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Human Pluripotent Stem Cells by Anonim Pdf

Author : Arlene Chiu,Mahendra S. Rao
Publisher : Springer Science & Business Media
Page : 455 pages
File Size : 52,9 Mb
Release : 2003-08-01
Category : Science
ISBN : 9781592594238

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Human Embryonic Stem Cells by Arlene Chiu,Mahendra S. Rao Pdf

A discussion of all the key issues in the use of human pluripotent stem cells for treating degenerative diseases or for replacing tissues lost from trauma. On the practical side, the topics range from the problems of deriving human embryonic stem cells and driving their differentiation along specific lineages, regulating their development into mature cells, and bringing stem cell therapy to clinical trials. Regulatory issues are addressed in discussions of the ethical debate surrounding the derivation of human embryonic stem cells and the current policies governing their use in the United States and abroad, including the rules and conditions regulating federal funding and questions of intellectual property.

Author : Andras Nagy,Kursad Andras,Kursad Turksen
Publisher : Unknown
Page : 368 pages
File Size : 46,6 Mb
Release : 2016
Category : Cytology
ISBN : 1493930346

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Patient-Specific Induced Pluripotent Stem Cell Models by Andras Nagy,Kursad Andras,Kursad Turksen Pdf

This volume captures the rapid developments in the field of induced pluripotent stem (iPS) cells, which have provided novel opportunities and approaches both for better understanding a number of human diseases and for developing new platforms for drug development and screening for such diseases. Specifically, representative protocols on various disease models have been collected from labs around the world. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Patient-Specific Induced Pluripotent Stem Cell Models: Generation and Characterization is an ideal reference for scientists working on furthering iPS research.

Author : Vivi M. Heine,Stephanie Dooves,Dwayne Holmes,Judith Wagner
Publisher : Springer Science & Business Media
Page : 69 pages
File Size : 41,6 Mb
Release : 2011-11-23
Category : Science
ISBN : 9789400728165

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Induced Pluripotent Stem Cells in Brain Diseases by Vivi M. Heine,Stephanie Dooves,Dwayne Holmes,Judith Wagner Pdf

Brain diseases can have a large impact on patients and society, and treatment is often not available. A new approach in which somatic cells are reprogrammed into induced pluripotent cells (iPS cells) is a significant breakthrough for regenerative medicine. This promises patient-specific tissue for replacement therapies, as well as disease-specific cells for developmental modeling and drug treatment screening. However, this method faces issues of low reprogramming efficiency, and poorly defined criteria for determining the conversion of one cell type to another. Cells contain epigenetic “memories” of what they were that can affect reprogramming. This book discusses the various methods to reprogram cells, the control and determination of cell identity, the epigenetic models that have emerged and the application of iPS cell therapy for brain diseases, in particular Parkinson’s disease and Vanishing White Matter (VWM).​

Author : Kuldip S. Sidhu
Publisher : Bentham Science Publishers
Page : 250 pages
File Size : 47,8 Mb
Release : 2012
Category : Science
ISBN : 9781608052899

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Frontiers in Pluripotent Stem Cells Research and Therapeutic Potentials Bench-to-Bedside by Kuldip S. Sidhu Pdf

"Pluripotent stem cells have garnered tremendous interest in recent years, which is primarily driven by the hope of finding a cure for several debilitating human diseases. Cell transplantation (regeneratve medicine) offers considerable therapeutic potentia"

Author : Michael J. Edel
Publisher : MDPI
Page : 422 pages
File Size : 40,5 Mb
Release : 2018-10-02
Category : Electronic book
ISBN : 9783038421221

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iPS Cells for Modelling and Treatment of Human Diseases by Michael J. Edel Pdf

This book is a printed edition of the Special Issue "iPS Cells for Modelling and Treatment of Human Diseases" that was published in JCM

Author : Mayana Zatz,Oswaldo Keith Okamoto
Publisher : Springer
Page : 147 pages
File Size : 49,7 Mb
Release : 2015-08-11
Category : Science
ISBN : 9783319183145

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Stem Cells in Modeling Human Genetic Diseases by Mayana Zatz,Oswaldo Keith Okamoto Pdf

While most stem cell books focus on basic aspects and/or cell therapy, this book emphasizes the relevance of stem cells obtained from patients, the so-called “patients in a petri dish” as tools to investigate human genetic diseases for which there are no available effective treatment. Chapters embrace several examples of the use of iPS cell technology, a recent Nobel Prize-winning scientific breakthrough, to obtain patient-specific pluripotent cells from which many types of specialized cells involved in a particular disease can be generated, including psychiatric and neurodegenerative disorders, muscular dystrophies, laminopathies, among others. The text is a current and timely resource for postgraduate students, scientists and clinicians, interested in applications of this rapidly developing field of research in disease modeling, drug development, and emerging issues that it brings to regenerative medicine.

Author : Haruhisa Inoue,Yukio Nakamura
Publisher : Springer
Page : 190 pages
File Size : 53,9 Mb
Release : 2019-04-01
Category : Medical
ISBN : 9789811336720

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Medical Applications of iPS Cells by Haruhisa Inoue,Yukio Nakamura Pdf

This volume highlights recent advances using iPS cells in disease modeling and medical applications along with new technologies that enhance the power of iPS cells. While the discovery of iPS cells has provided excellent opportunities for developing models of human diseases, platforms for drug discovery and cell therapies, iPS cell technology still faces many challenges. Presenting the latest advances in this rapidly evolving research area, this book is intended to widen the community of researchers and clinicians interested in the exciting field of iPS cells.

Author : Sibel Yildirim
Publisher : Springer Science & Business Media
Page : 76 pages
File Size : 51,9 Mb
Release : 2012-02-02
Category : Science
ISBN : 9781461422068

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Induced Pluripotent Stem Cells by Sibel Yildirim Pdf

Induced Pluripotent Stem (iPS) cells are mature cells that have been genetically reprogrammed so that they return to their embryonic state. It is not yet known if iPS stem cells and embryonic stem cells differ significantly. Today many fundamental belief systems in biology are shifting towards accepting that mature body cells can be reverted to an embryonic state without the help of eggs or embryos. With their changed identities, iPS cells are then ready to serve as new tools for research in the fields of disease pathogenesis, drug discovery, oncology, and cell transplantation. One example of this would be using iPS from a patient’s mature cells to repair damaged tissue; it is thought that there would be very low incidence of rejection of the ‘new’ tissue in these cases. For the last four years, this therapeutic promise has been studied by hundreds of researchers worldwide in an effort to understand the ability of these cells to reverse their biological clocks.

Author : Deepak A. Lamba
Publisher : CRC Press
Page : 280 pages
File Size : 47,5 Mb
Release : 2017-04-07
Category : Medical
ISBN : 9781315402178

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Patient-Specific Stem Cells by Deepak A. Lamba Pdf

One of the biggest challenges faced in medical research had been to create accurate and relevant models of human disease. A number of good animal models have been developed to understand the pathophysiology. However, not all of them reflect the human disorder, a classic case being Usher’s syndrome where the mutant mice do not have the same visual and auditory defects that patients face. There are others which have been even more difficult to model due to the multi-factorial nature of the condition and due to lack of discovery of a single causative gene such as age-related macular degeneration or Alzheimer’s syndrome. Thus a more relevant and accurate system will allow us to make better predictions on relevant therapeutic approaches. The discovery of human pluripotent stem cells in 1998 followed by the technological advances to reprogram somatic cells to pluripotent-stem cell-like cells in 2006 has completely revolutionized the way we can now think about modelling human development and disease. This now coupled with genome editing technologies such as TALENS and CRISPRs have now set us up to develop in vitro models both 2D as well as 3D organoids, which can more precisely reflect the disease in the patients. These combinatorial technologies are already providing us with better tools and therapeutics in drug discovery or gene therapy. This book summarizes both the technological advances in the field of generation of patient specific lines as well as various gene editing approaches followed by its applicability in various systems. The book will serve as a reference for the current state of the field as it: -Provides a comprehensive overview of the status of the field of patients derived induced pluripotent stem cells. -Describes the use of cardiac cells as a main featured component within the book. -Examines drug toxicity analysis as a working example throughout the book.

Author : M.A. Hayat
Publisher : Springer Science & Business Media
Page : 284 pages
File Size : 54,9 Mb
Release : 2014-02-20
Category : Medical
ISBN : 9789401780322

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Stem Cells and Cancer Stem Cells, Volume 12 by M.A. Hayat Pdf

Volume 12 in this series explores the latest experimental and clinical uses of stem cells in the treatment of disease and of injuries and reviews methods for isolating multipotent endothelial-like cells from human adipose tissue and discusses clinical applications in cell therapy and regenerative medicine. The book is organized in five parts: Cancer Stem Cells, Pluripotent Stem Cells, Dendritic Stem Cells, Regenerative Medicine and General Applications. The first section includes chapters on histamine in the neural and cancer stem cell niches and emerging concepts of stem cell organization in the normal lung and in lung cancer. The section on Pluripotent Stem Cells includes discussion of the differentiation of dendritic cells from human induced pluripotent stem cell and the molecular mechanisms involved in reprogramming human somatic cells to generate induced pluripotent stem cells. Additional chapters cover the differentiation of induced pluripotent stem cells into functional cardiomyocytes, characteristics of satellite cells and multipotent adult stem cells in the skeletal muscle. The section on Dendritic Stem Cells explores the critical role of notch signaling in the differentiation and function of dendrite. Other chapters cover hypertensive emergencies in children after stem cell transplantation and overcoming the radio resistance of lung cancer stem cells. The section on Regenerative Medicine reports on experiments on improved renal revascularization in pigs using stem cells and phenotypic correction of murine Hemophilia A using cell-based therapy. The concluding section, General Applications, discusses such topics as methods in mathematical modeling for stem cells, as well as molecular and functional characterization of human adipocytes. Like its eleven predecessors in the series, this volume stands out for its comprehensive approach, its roster of some 51 expert contributors representing a dozen different countries and its up-to-date review of leading-edge technology and methods.