Interferon Methods And Protocols

Author : Daniel J. J. Carr
Publisher : Springer Science & Business Media
Page : 239 pages
File Size : 43,9 Mb
Release : 2008-02-02
Category : Medical
ISBN : 9781592599394

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Interferon Methods and Protocols by Daniel J. J. Carr Pdf

A compendium of optimized methods to measure type I interferon efficacy as an antiproliferative or an antiviral agent. These cutting-edge techniques range from the simple to the highly complex and serve to illuminate the signaling cascades and the activation of enzymatic pathways prompted by interferon. The protocols follow the successful Methods in Molecular MedicineTM series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. State-of-the-art and highly practical, Interferon Methods and Protocols offers researchers powerful tools not only to ascertain the functions of IFN-stimulatory gene products, but also to identify additional molecular pathways that will clarify our understanding of the many biological events influenced by IFNs.

Author : Meral Beksaç
Publisher : Springer Science & Business Media
Page : 485 pages
File Size : 55,6 Mb
Release : 2007-05-03
Category : Medical
ISBN : 9781588295958

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Bone Marrow and Stem Cell Transplantation by Meral Beksaç Pdf

This volume is a compendium of cutting-edge molecular methods for the successful transplantation of hematopoietic stem cells. The contributors are world-renown leaders in the field. They describe promising tools for stem cell transplant research models, such as in vivo bioluminescence imaging. They discuss HLA typing, PCR-SSP typing, and HLA antigens. This volume is an invaluable source for biochemists, molecular biologists, and clinicians.

Author : Helena J. Maier,Erica Bickerton
Publisher : Humana
Page : 278 pages
File Size : 45,7 Mb
Release : 2021-09-08
Category : Medical
ISBN : 1071609025

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Coronaviruses by Helena J. Maier,Erica Bickerton Pdf

This detailed new edition provides a comprehensive collection of protocols applicable to all members of the Coronavirinae sub-family currently and that are also transferrable to other fields of virology. Beginning with a section on detection, discovery, and evolution, the volume continues with coverage of propagation and titration of coronaviruses, genome manipulation, study of virus-host interactions, as well as imaging coronavirus infections. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Coronaviruses: Methods and Protocols, Second Edition serves as a valuable guide to researchers working to identify and control viruses with increased potential to cross the species barrier and to develop the diagnostics, vaccines, and antiviral therapeutics that are required to manage future outbreaks in both humans and animals.

Author : Irving C. Allen
Publisher : Humana
Page : 0 pages
File Size : 48,7 Mb
Release : 2016-08-27
Category : Medical
ISBN : 1493960369

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Mouse Models of Innate Immunity by Irving C. Allen Pdf

The innate immune system represents a critical arm of the immune response by providing immediate and robust host defense; however, human studies of its function are often limited by ethical, logistical, and technical obstacles. In Mouse Models of Innate Immunity: Methods and Protocols, experts in the field explore the design and execution of experiments used to thoroughly evaluate critical elements associated with the host innate immune response. The volume opens with methods that are essential for collecting and assessing various primary cells that are highly relevant to innate immunity, and it continues with in vivo protocols commonly used to evaluate the innate immune response in the mouse, including mouse models of respiratory infection, gastrointestinal inflammation, fungal and parasitic diseases, sepsis, and HIV-1 infection. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and easy to use, Mouse Models of Innate Immunity: Methods and Protocols will serve the research community by providing expert advice and protocols that allow both experienced and novice investigators to successfully plan, implement, and assess disease processes associated with the innate immune response.

Author : Paul Eggleton,Frank J. Ward
Publisher : Humana
Page : 0 pages
File Size : 48,7 Mb
Release : 2016-08-23
Category : Medical
ISBN : 1493952625

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Systemic Lupus Erythematosus by Paul Eggleton,Frank J. Ward Pdf

Systemic Lupus Erythematosus: Methods and Protocols describe a number of genetic, biochemical and immunological techniques. These techniques provide an advancing understanding of the pathology, breakdown of the immune system and therapeutic challenges of SLE in both humans and animal models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Systemic Lupus Erythematosus: Methods and Protocols appeal to biomedical and clinical scientists in a number of pathology disciplines at the doctoral and post-doctoral level.

Author : Catherine H. Schein
Publisher : Springer Science & Business Media
Page : 521 pages
File Size : 51,6 Mb
Release : 2008-02-03
Category : Science
ISBN : 9781592592333

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Nuclease Methods and Protocols by Catherine H. Schein Pdf

Nucleases, enzymes that restructure or degrade nucleic acid polymers, are vital to the control of every area of metabolism. They range from “housekeeping” enzymes with broad substrate ranges to extremely specific tools (1). Many types of nucleases are used in lab protocols, and their commercial and clinical uses are expanding. The purpose of Nuclease Methods and Protocols is to introduce the reader to some we- characterized protein nucleases, and the methods used to determine their activity, structure, interaction with other molecules, and physiological role. Each chapter begins with a mini-review on a specific nuclease or a nuclease-related theme. Although many chapters cover several topics, they were arbitrarily divided into five parts: Part I, “Characterizing Nuclease Activity,” includes protocols and assays to determine general (processive, distributive) or specific mechanisms. Methods to assay nuclease products, identify cloned nucleases, and determine their physiological role are also included here. Part II, “Inhibitors and Activators of Nucleases,” summarizes assays for measuring the effects of other proteins and small molecules. Many of these inhibitors have clinical relevance. Part III, “Relating Nuclease Structure and Function,” provides an overview of methods to determine or model the 3-D structure of nucleases and their complexes with substrates and inhibitors. A 3-D structure can greatly aid the rational design of nucleases and inhibitors for specific purposes. Part IV, “Nucleases in the Clinic,” summarizes assays and protocols suitable for use with t- sues and for nuclease based therapeutics.

Author : Anonim
Publisher : Gulf Professional Publishing
Page : 402 pages
File Size : 41,5 Mb
Release : 1975
Category : Electronic
ISBN : 0121820408

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Cumulative Subject Index [to] Methods in Enzymology by Anonim Pdf

Author : Karol Sikora
Publisher : Springer Science & Business Media
Page : 212 pages
File Size : 48,9 Mb
Release : 2013-11-11
Category : Medical
ISBN : 9781468411706

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Interferon and Cancer by Karol Sikora Pdf

Interferon was first discovered in 1957. Over the last five years it has become almost a household word. Many believe it to be a drug with already proven efficacy against cancer and viral infection. The media has distorted any cool scientific view of the data available. We have learned much about the complexity of the interferon system. We know some of the switches involved in interferon gene expression and its secretion by virally and immune stimulated cells. We also know that it binds to a cell surface receptor, mediating its complex effects on target cells by a series of second messengers. The advent of the new techniques of modern molecular biology, such as monoclonal antibodies and gene cloning, has had tremendous impact on the rate of acquisition of knowledge. Such techniques have provided us with almost unlimited quantities of highly purified interferon for clinical trial in patients with a spectrum of infectious and malignant diseases. The information we have gathered raises many more questions. Why should there be several families of interferon genes? What is their true physiolo gical role? How are they interrelated functionally? Interferon is clearly a cellular hormone providing a means of communication between cells. ~fhether it has clinical value in the management of patients with diseased cells remains to be seen. This book sum marises our current knowledge of interferons as possible an- cancer agents. It is clear interferon is no penicillin for cancer.

Author : Anonim
Publisher : Unknown
Page : 1216 pages
File Size : 46,5 Mb
Release : 1986
Category : Medicine
ISBN : OSU:32436011073077

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Cumulated Index Medicus by Anonim Pdf

Author : J. Philip McCoy, Jr
Publisher : Humana
Page : 356 pages
File Size : 44,8 Mb
Release : 2020-09-15
Category : Medical
ISBN : 1493996525

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Immunophenotyping by J. Philip McCoy, Jr Pdf

This volume presents the latest collection of immunophenotypic techniques and applications used in research and clinical settings. Chapters in this book cover topics such as constructions of high dimensions fluorescence and mass cytometry panels; fluorescence barcoding; using dried or lyophilized reagents; and immunophenotypic examples of specific cell types. The book concludes with a discussion on the critical roles of quality control and immunophenotyping in the clinical environment. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Immunophenotyping: Methods and Protocols is a valuable resource for any researchers, clinician, or scientist interested in learning more about this evolving field.

Author : Shree Ram Singh,Robert M. Hoffman,Amit Singh
Publisher : Humana
Page : 217 pages
File Size : 42,9 Mb
Release : 2021-02-20
Category : Science
ISBN : 1071610074

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Mouse Genetics by Shree Ram Singh,Robert M. Hoffman,Amit Singh Pdf

This fully updated edition provides selected mouse genetic techniques and their application in modeling varieties of human diseases. The chapters are mainly focused on the generation of different transgenic mice to accomplish the manipulation of genes of interest, tracing cell lineages, and modeling human diseases. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Mouse Genetics: Methods and Protocols, Second Edition delivers fundamental techniques and protocols to geneticists, molecular biologists, cell and developmental biologists, students, and postdoctoral fellows working in the various disciplines of genetics, developmental biology, mouse genetics, and modeling human diseases.

Author : Sigma Chemical Company
Publisher : Unknown
Page : 3100 pages
File Size : 45,9 Mb
Release : 2006
Category : Biological reagents
ISBN : PSU:000056810202

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Biochemicals, Reagents & Kits for Life Science Research by Sigma Chemical Company Pdf

Author : Maria S. Salvato
Publisher : Humana Press
Page : 425 pages
File Size : 55,6 Mb
Release : 2017-10-07
Category : Medical
ISBN : 1493969803

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Hemorrhagic Fever Viruses by Maria S. Salvato Pdf

This volume presents protocols that analyze and explore hemorrhagic fever viruses (HFV). This book is divided into 5 parts: Part I begins with an overview on predicting viral pandemics and then covers methods for surveillance, diagnosis, and classification of HFV. This includes an antibody capture method using Lassa virus antigens. Part II discusses structural studies and reverse genetics of HFV. The chapters in this part describe envelope glycoprotein membrane fusion studies, arenavirus nucleocapsid protein, and the use of virus-like-particles to study viral egress. Part III explores in vivo models of HFV infections, and contains chapters on murine, guinea pig, and primate models for HFV, and methods to obtain a subset of primary human liver cells that can be cultured long-term. Part IV looks into immune assays and vaccine production for HFV. The chapters in this section cover the attenuated vaccine for Argentine HFV, detecting virus-antibody immune complexes in secondary dengue infections, and DNA vaccination. Part V discusses host responses to viral hemorrhagic fever, and contains chapters on identifying host restrictions to Junín or Dengue infection, and a cell-culture method to assess coagulation after HFV infection. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Hemorrhagic Fever Viruses: Methods and Protocols is a valuable resource for scientists and researchers who want to bridge the gap between virus recognition in surveillance and understanding host responses to infection.

Author : Anthony Meager
Publisher : John Wiley & Sons
Page : 460 pages
File Size : 45,7 Mb
Release : 2006-08-21
Category : Science
ISBN : 9783527607921

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The Interferons by Anthony Meager Pdf

At long last, the first book to cover all important areas of interferon science in one volume. Top scientists, including many pioneers in the field, highlight the role of interferons as research tools and as therapeutic agents in clinical applications. Edited by an experienced interferonologist, chapters include discussions of interferon genes, Type I, II and III IFNs, as well as their induction, production and purification, receptors actions, measuring IFN activities and anti-IFN antibodies, as well as the evolution of viral defense mechanisms. For immunologists, cancer researchers, medicinal chemists, cell biologists, developmental biologists and the pharmaceutical industry.

Author : Shunzo Chiba,Mary K. Estes,Shuji Nakata,Charles H. Calisher
Publisher : Springer Science & Business Media
Page : 301 pages
File Size : 40,8 Mb
Release : 2012-12-06
Category : Medical
ISBN : 9783709165539

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Viral Gastroenteritis by Shunzo Chiba,Mary K. Estes,Shuji Nakata,Charles H. Calisher Pdf

Recently, rapid developments have occurred in the field of viral gastroenteritis. This book is an update of fundamental and practical aspects of viral gastroenteritis. Among the various agents that cause viral gastroenteritis, group A rotaviruses and caliciviruses are the focus of this volume because of their clinical impact and the significance of new findings about them.