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Introduction to Plant Biotechnology (3/e) by H S Chawla Pdf
This book has been written to meet the needs of students for biotechnology courses at various levels of undergraduate and graduate studies. This book covers all the important aspects of plant tissue culture viz. nutrition media, micropropagation, organ culture, cell suspension culture, haploid culture, protoplast isolation and fusion, secondary metabolite production, somaclonal variation and cryopreservation. For good understanding of recombinant DNA technology, chapters on genetic material, organization of DNA in the genome and basic techniques involved in recombinant DNA technology have been added. Different aspects on rDNA technology covered gene cloning, isolation of plant genes, transposons and gene tagging, in vitro mutagenesis, PCR, molecular markers and marker assisted selection, gene transfer methods, chloroplast and mitochondrion DNA transformation, genomics and bioinformatics. Genomics covers functional and structural genomics, proteomics, metabolomics, sequencing status of different organisms and DNA chip technology. Application of biotechnology has been discussed as transgenics in crop improvement and impact of recombinant DNA technology mainly in relation to biotech crops.
Introduction to Plant Biotechnology by H. S. Chawla Pdf
Plant biotechnology has created unprecedented opportunities for the manipulation of biological systems of plants. To understand biotechnology, it is essential to know the basic aspects of genes and their organization in the genome of plant cells. This text on the subject is aimed at students.
Basics; Laboratory organization; Sterilization techniques; Nutrition medium; Choice of the explant; Plant tissue culture; Seed culture; Micropropagation- meristem culture; Micropropagation- axillary bud proliferation; Micropropagation- adventitious regeneration; Micropropagation- organogenesis; Micropropagation- embryogenesis; Cell suspension; Secondary metabolite production in a cell suspension culture; Anther culture; Protoplast isolation and fusion; Biotechnology; SDS-PAGE electrophoresis of proteins; Isolation of DNA from plant tissues; Isolation an purification of plasmid DNA; Restriction enzyme digestion of DNA; Agarose gel electrophoresis; Preparation of competent cells, transformation of E. coil with plasmid DNA and ligation of insert DNA to a vector; Agrobacterium-mediated gene transfer; Biolistic method of transformation in plants; In vitro amplification of DNA by PCR: detection of transgenes; RAPD analysis; Microsatellite marker analysis; Southerm biotting; Southerm hybridization.
Plant Biotechnology by Adrian Slater,Nigel Scott,Mark Fowler Pdf
Plant Biotechnology presents a balanced, objective exploration of the technology behind genetic manipulation, and its application to the growth and cultivation of plants. The book describes the techniques underpinning genetic manipulation and makes extensive use of case studies to illustrate how this influential tool is used in practice.
Plant Biotechnology by Agnès Ricroch,Surinder Chopra,Marcel Kuntz Pdf
Written in easy to follow language, the book presents cutting-edge agriculturally relevant plant biotechnologies and applications in a manner that is accessible to all. This book updates and introduces the scope and method of plant biotechnologies and molecular breeding within the context of environmental analysis and assessment, a diminishing supply of productive arable land, scarce water resources and climate change. New plant breeding techniques including CRISPR-cas system are now tools to meet these challenges both in developed countries and in developing countries. Ethical issues, intellectual property rights, regulation policies in various countries related to agricultural biotechnology are examined. The rapid developments in plant biotechnology are explained to a large audience with relevant examples. New varieties of crops can be adapted to new climatic conditions in order to reduce pest-associated losses and the adverse abiotic effects
Plant Biotechnology and Genetics by C. Neal Stewart, Jr. Pdf
Designed to inform and inspire the next generation of plant biotechnologists Plant Biotechnology and Genetics explores contemporary techniques and applications of plant biotechnology, illustrating the tremendous potential this technology has to change our world by improving the food supply. As an introductory text, its focus is on basic science and processes. It guides students from plant biology and genetics to breeding to principles and applications of plant biotechnology. Next, the text examines the critical issues of patents and intellectual property and then tackles the many controversies and consumer concerns over transgenic plants. The final chapter of the book provides an expert forecast of the future of plant biotechnology. Each chapter has been written by one or more leading practitioners in the field and then carefully edited to ensure thoroughness and consistency. The chapters are organized so that each one progressively builds upon the previous chapters. Questions set forth in each chapter help students deepen their understanding and facilitate classroom discussions. Inspirational autobiographical essays, written by pioneers and eminent scientists in the field today, are interspersed throughout the text. Authors explain how they became involved in the field and offer a personal perspective on their contributions and the future of the field. The text's accompanying CD-ROM offers full-color figures that can be used in classroom presentations with other teaching aids available online. This text is recommended for junior- and senior-level courses in plant biotechnology or plant genetics and for courses devoted to special topics at both the undergraduate and graduate levels. It is also an ideal reference for practitioners.
Plant Biotechnology and Agriculture by Arie Altman,Paul Michael Hasegawa Pdf
As the oldest and largest human intervention in nature, the science of agriculture is one of the most intensely studied practices. From manipulation of plant gene structure to the use of plants for bioenergy, biotechnology interventions in plant and agricultural science have been rapidly developing over the past ten years with immense forward leaps on an annual basis. This book begins by laying the foundations for plant biotechnology by outlining the biological aspects including gene structure and expression, and the basic procedures in plant biotechnology of genomics, metabolomics, transcriptomics and proteomics. It then focuses on a discussion of the impacts of biotechnology on plant breeding technologies and germplasm sustainability. The role of biotechnology in the improvement of agricultural traits, production of industrial products and pharmaceuticals as well as biomaterials and biomass provide a historical perspective and a look to the future. Sections addressing intellectual property rights and sociological and food safety issues round out the holistic discussion of this important topic. Includes specific emphasis on the inter-relationships between basic plant biotechnologies and applied agricultural applications, and the way they contribute to each other Provides an updated review of the major plant biotechnology procedures and techniques, their impact on novel agricultural development and crop plant improvement Takes a broad view of the topic with discussions of practices in many countries
An Introduction to Molecular Biotechnology by Michael Wink Pdf
Molecular biotechnology continues to triumph, as this textbook testifies - edited by one of the academic pioneers in the field and written by experienced professionals. This completely revised second edition covers the entire spectrum, from the fundamentals of molecular and cell biology, via an overview of standard methods and technologies, the application of the various "-omics", and the development of novel drug targets, right up to the significance of system biology in biotechnology. The whole is rounded off by an introduction to industrial biotechnology as well as chapters on company foundation, patent law and marketing. The new edition features: - Large format and full color throughout - Proven structure according to basics, methods, main topics and economic perspectives - New sections on system biology, RNA interference, microscopic techniques, high throughput sequencing, laser applications, biocatalysis, current biomedical applications and drug approval - Optimized teaching with learning targets, a glossary containing around 800 entries, over 500 important abbreviations and further reading. The only resource for those who are seriously interested in the topic. Bonus material available online free of charge: www.wiley-vch.de/home/molecbiotech
Author : William G. Hopkins,Norman P. A. Hüner Publisher : John Wiley & Sons Page : 536 pages File Size : 47,5 Mb Release : 2008-12-10 Category : Science ISBN : STANFORD:36105132266805
Plant Cell and Tissue Culture - A Tool in Biotechnology by Karl-Hermann Neumann,Ashwani Kumar,Jafargholi Imani Pdf
This book provides a general introduction as well as a selected survey of key advances in the fascinating field of plant cell and tissue culture as a tool in biotechnology. After a detailed description of the various basic techniques employed in leading laboratories worldwide, follows an extended account of important applications in, for example, plant propagation, secondary metabolite production and gene technology. Additionally, some chapters are devoted to historical developments in this domain, metabolic aspects, nutrition, growth regulators, differentiation and the development of culture systems. The book will prove useful to both newcomers and specialists, and even “old hands” in tissue culture should find some challenging ideas to think about.
Plant Genetics and Molecular Biology by Rajeev K. Varshney,Manish K. Pandey,Annapurna Chitikineni Pdf
This book reviews the latest advances in multiple fields of plant biotechnology and the opportunities that plant genetics, genomics and molecular biology have offered for agriculture improvement. Advanced technologies can dramatically enhance our capacity in understanding the molecular basis of traits and utilizing the available resources for accelerated development of high yielding, nutritious, input-use efficient and climate-smart crop varieties. In this book, readers will discover the significant advances in plant genetics, structural and functional genomics, trait and gene discovery, transcriptomics, proteomics, metabolomics, epigenomics, nanotechnology and analytical & decision support tools in breeding. This book appeals to researchers, academics and other stakeholders of global agriculture.
Date Palm Biotechnology by Shri Mohan Jain,Jameel M. Al-Khayri,Dennis V. Johnson Pdf
This important reference book is the first comprehensive resource worldwide that reflects research achievements in date palm biotechnology, documenting research events during the last four decades, current status, and future outlook. This book is essential for researchers, policy makers, and commercial entrepreneurs concerned with date palm. The book is invaluable for date palm biotechnology students and specialists. This monument is written by an international team of experienced researchers from both academia and industry. It consists of five sections covering all aspects of date palm biotechnology including A) Micropropagation, B) Somaclonal Variation, Mutation and Selection, C) Germplasm Biodiversity and Conservation, D) Genetics and Genetic Improvement, and E) Metabolites and Industrial Biotechnology. The book brings together the principles and practices of contemporary date palm biotechnology. Each chapter contains background knowledge related to the topic, followed by a comprehensive literature review of research methodology and results including the authors own experience including illustrative tables and photographs.
Applied Plant Genomics and Biotechnology by Palmiro Poltronieri,Yiguo Hong Pdf
Applied plant genomics and biotechnology reviews the recent advancements in the post-genomic era, discussing how different varieties respond to abiotic and biotic stresses, investigating epigenetic modifications and epigenetic memory through analysis of DNA methylation states, applicative uses of RNA silencing and RNA interference in plant physiology and in experimental transgenics, and plants modified to produce high-value pharmaceutical proteins. The book provides an overview of research advances in application of RNA silencing and RNA interference, through Virus-based transient gene expression systems, Virus induced gene complementation (VIGC), Virus induced gene silencing (Sir VIGS, Mr VIGS) Virus-based microRNA silencing (VbMS) and Virus-based RNA mobility assays (VRMA); RNA based vaccines and expression of virus proteins or RNA, and virus-like particles in plants, the potential of virus vaccines and therapeutics, and exploring plants as factories for useful products and pharmaceuticals are topics wholly deepened. The book reviews and discuss Plant Functional Genomic studies discussing the technologies supporting the genetic improvement of plants and the production of plant varieties more resistant to biotic and abiotic stresses. Several important crops are analysed providing a glimpse on the most up-to-date methods and topics of investigation. The book presents a review on current state of GMO, the cisgenesis-derived plants and novel plant products devoid of transgene elements, discuss their regulation and the production of desired traits such as resistance to viruses and disease also in fruit trees and wood trees with long vegetative periods. Several chapters cover aspects of plant physiology related to plant improvement: cytokinin metabolism and hormone signaling pathways are discussed in barley; PARP-domain proteins involved in Stress-Induced Morphogenetic Response, regulation of NAD signaling and ROS dependent synthesis of anthocyanins. Apple allergen isoforms and the various content in different varieties are discussed and approaches to reduce their presence. Euphorbiaceae, castor bean, cassava and Jathropa are discussed at genomic structure, their diseases and viruses, and methods of transformation. Rice genomics and agricultural traits are discussed, and biotechnology for engineering and improve rice varieties. Mango topics are presented with an overview of molecular methods for variety differentiation, and aspects of fruit improvement by traditional and biotechnology methods. Oilseed rape is presented, discussing the genetic diversity, quality traits, genetic maps, genomic selection and comparative genomics for improvement of varieties. Tomato studies are presented, with an overview on the knowledge of the regulatory networks involved in flowering, methods applied to study the tomato genome-wide DNA methylation, its regulation by small RNAs, microRNA-dependent control of transcription factors expression, the development and ripening processes in tomato, genomic studies and fruit modelling to establish fleshy fruit traits of interest; the gene reprogramming during fruit ripening, and the ethylene dependent and independent DNA methylation changes. provides an overview on the ongoing projects and activities in the field of applied biotechnology includes examples of different crops and applications to be exploited reviews and discusses Plant Functional Genomic studies and the future developments in the field explores the new technologies supporting the genetic improvement of plants
Plant Biotechnology and Genetics by C. Neal Stewart, Jr. Pdf
Focused on basics and processes, this textbook teaches plant biology and agriculture applications with summary and discussion questions in each chapter. Updates each chapter to reflect advances / changes since the first edition, for example: new biotechnology tools and advances, genomics and systems biology, intellectual property issues on DNA and patents, discussion of synthetic biology tools Features autobiographical essays from eminent scientists, providing insight into plant biotechnology and careers Has a companion website with color images from the book and PowerPoint slides Links with author's own website that contains teaching slides and graphics for professors and students: http://bit.ly/2CI3mjp
