Introduction To Plant Cell Tissue And Organ Culture

Author : SUNIL D. PUROHIT
Publisher : PHI Learning Pvt. Ltd.
Page : 334 pages
File Size : 53,8 Mb
Release : 2012-10-30
Category : Science
ISBN : 9788120346772

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INTRODUCTION TO PLANT CELL TISSUE AND ORGAN CULTURE by SUNIL D. PUROHIT Pdf

Designed primarily as a text for undergraduate and postgraduate students of Botany and Plant Biotechnology, the book discusses the theoretical aspects and modern applications of plant cell, tissue and organ culture. Written with the aim of providing up-to-date information on the subject, and focused on the concept of commercialization of plant cell culture, the contents have been presented with clarity. The book not only discusses the theoretical aspects of plant tissue culture but also emphasizes the art of its practice. It also provides a systematic explanation of asepsis and methods of sterilization, plant tissue culture techniques, culture of reproductive structures, plant tissue culture in germplasm conservation, its applications in the industry and plant pathology and operation and management of greenhouse hardening unit. In addition, it discusses in vitro propagation of plants (micropropagation) with a series of case studies pertaining to tree species and horticultural crops. Besides students, the book will also prove to be useful for researchers, scholars and teachers.

Author : Oluf Gamborg,Gregory C. Phillips
Publisher : Springer Science & Business Media
Page : 367 pages
File Size : 47,9 Mb
Release : 2013-06-29
Category : Science
ISBN : 9783642790485

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Plant Cell, Tissue and Organ Culture by Oluf Gamborg,Gregory C. Phillips Pdf

This manual provides all relevant protocols for basic and applied plant cell and molecular technologies, such as histology, electron microscopy, cytology, virus diagnosis, gene transfer and PCR. Also included are chapters on laboratory facilities, operation and management as well as a glossary and all the information needed to set up and carry out any of the procedures without having to use other resource books. It is especially designed for professionals and advanced students who wish to acquire practical skills and first-hand experience in plant biotechnology.

Author : Jakob Reinert,Yashpal S. Bajaj
Publisher : Springer Science & Business Media
Page : 810 pages
File Size : 48,9 Mb
Release : 2013-04-17
Category : Science
ISBN : 9783662022795

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Applied and Fundamental Aspects of Plant Cell, Tissue, and Organ Culture by Jakob Reinert,Yashpal S. Bajaj Pdf

Progress in the field of plant cell and tissue culture has made this area of research one of the most dynamic and promising not only in plant physiology, cell biology and genetics but also in agriculture, forestry, horticulture and industry. Studies with plant cell cultures clearly have bearing upon a variety of problems as yet unsolved in basic and applied research. This was the compelling reason for assembling such a comprehensive source of information to stimulate students, teachers, and research workers. This book comprises 34 articles on regeneration of plants, vegetative propagation and cloning; haploids; cytology, cytogenetics and plant breeding; protoplasts, somatic hybridization and genetic engineering; plant pathology; secondary products and a chapter on isoenzymes, radiobiology, and cryobiology of plant cells. Particular attention has been paid to modern , fast-growing and fascinating disciplines - e.g. the induction of haploids, somatic hybridization and genetic manipulation by protoplast culture, which possess an enormous potential for plant improvement.

Author : Gamborg
Publisher : Unknown
Page : 128 pages
File Size : 43,8 Mb
Release : 1995
Category : Electronic
ISBN : 3662586703

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Plant Cell, Tissue and Organ Culture by Gamborg Pdf

Author : O. L.(Eds.) Gamborg,G. C. Philips
Publisher : Unknown
Page : 348 pages
File Size : 47,5 Mb
Release : 1998
Category : Plant cell culture
ISBN : OCLC:786431629

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Plant Cell Tissue and Organ Culture-Fundamental Methods by O. L.(Eds.) Gamborg,G. C. Philips Pdf

Author : Indra K. Vasil,Trevor A. Thorpe
Publisher : Springer Science & Business Media
Page : 593 pages
File Size : 40,8 Mb
Release : 2013-03-09
Category : Science
ISBN : 9789401726818

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Plant Cell and Tissue Culture by Indra K. Vasil,Trevor A. Thorpe Pdf

Plant Cell and Tissue Culture gives an exhaustive account of plant cell culture and genetic transformation, including detailed chapters on all major field and plantation crops. Part A presents a comprehensive coverage of all necessary laboratory techniques for the initiation, nutrition, maintenance and storage of plant cell and tissue cultures, including discussions on these topics, as well as on morphogenesis and regeneration, meristem and shoot tip culture, plant protoplasts, mutant cell lines, variation in tissue cultures, isogenic lines, fertilization control, cryopreservation, transformation, and the production of secondary metabolites. Part B then proceeds into detail on the specific in vitro culture of specific crops, including cereals, legumes, vegetables, potatoes, other roots and tubers, oilseeds, temperate fruits, tropical fruits, plantation crops, forest trees and ornamentals. Plant Cell and Tissue Culture is, and is likely to remain, the laboratory manual of choice, as well as a source of inspiration and a guide to all workers in the field.

Author : M. K. Razdan
Publisher : Science Publishers
Page : 394 pages
File Size : 45,7 Mb
Release : 2003
Category : Science
ISBN : 1578082374

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Introduction to Plant Tissue Culture by M. K. Razdan Pdf

Introduction and techniques; Introductory history; Laboratory organisation; Media; Aseptic manipulation; Basic aspects; Cell culture; Cellular totipotency; Somatic embryogenesis; Applications to plant breeding; Haploid prodution; Triploid production; In vitro pollination and fertilization; Zygotic embryo culture; Somatic hybridisation and cybridisation; Genetic transformation; Somaclonal and gametoclonal variant selection; Application to horticulture and forestry; Production of disease-free plants; clonal propagation; General applications; Industrial applications: secondary metabolite production; Germplasm conservation.

Author : Michael R. Davey,Paul Anthony
Publisher : John Wiley & Sons
Page : 358 pages
File Size : 54,7 Mb
Release : 2010-05-20
Category : Science
ISBN : 0470686510

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Plant Cell Culture by Michael R. Davey,Paul Anthony Pdf

The ability to culture cells is fundamental for mass propagation and as a baseline for the genetic manipulation of plant nuclei and organelles. The introduction to Plant Cell Culture: Essential Methods provides a general background to plant cell culture, including basic principles, technologies and laboratory practices that underpin the more detailed techniques described in subsequent chapters. Whilst each chapter provides a background to the topic area and methodology, a crucial aspect is the provision of detailed protocols with emphasis on trouble shooting, describing common problems and detailed advice for their avoidance. Plant Cell Culture: Essential Methods provides the reader with a concise overview of these techniques, including micropropagation, mutagenesis, cryopreservation, genetic and plastid transformation and somatic cell technologies. This book will be an essential addition to any plant science laboratory's bookshelf. Highlights the best and most up-to-date techniques for working on plant cell culture Explains clearly and precisely how to carry out selected techniques in addition to background information on the various approaches Chapters are written by leading international authorities in the field and cover both well-known and new, tried and tested, methods for working in plant cell culture An essential laboratory manual for students and early-career researchers.

Author : E. N. Willmer
Publisher : Elsevier
Page : 843 pages
File Size : 55,6 Mb
Release : 2013-10-02
Category : Science
ISBN : 9781483277233

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Cells and Tissues in Culture Methods, Biology and Physiology by E. N. Willmer Pdf

Cells and Tissues in Culture: Methods, Biology, and Physiology, Volume 3 focuses on the applications of the methods of tissue culture to various fields of investigation, including virology, immunology, and preventive medicine. The selection first offers information on molecular organization of cells and tissues in culture and tissue culture in radiobiology. Topics include cellular organization at the molecular level, fibrogenesis in tissue culture, effect of radiation on the growth of isolated cells, and irradiation of the selected parts of the cell. The publication then considers the effects of invading organisms on cells and tissues in culture and cell, tissue, and organ cultures in virus research. The book elaborates on antibody production in tissue culture and tissue culture in pharmacology. Discussions focus on early attempts at in vitro studies, tissue culture in the study of pharmacologically active agents, and methods of assessment of drug activity. The text also reviews invertebrate tissue and organ culture in cell research; introduction and methods employed in plant tissue culture; and growth, differentiation and organogenesis in plant tissue and organ cultures. The selection is a vital source of data for readers interested in the culture of cells and tissues.

Author : Saurabh Bhatia,Kiran Sharma,Randhir Dahiya,Tanmoy Bera
Publisher : Academic Press
Page : 452 pages
File Size : 53,6 Mb
Release : 2015-07-22
Category : Science
ISBN : 9780128024980

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Modern Applications of Plant Biotechnology in Pharmaceutical Sciences by Saurabh Bhatia,Kiran Sharma,Randhir Dahiya,Tanmoy Bera Pdf

Modern Applications of Plant Biotechnology in Pharmaceutical Sciences explores advanced techniques in plant biotechnology, their applications to pharmaceutical sciences, and how these methods can lead to more effective, safe, and affordable drugs. The book covers modern approaches in a practical, step-by-step manner, and includes illustrations, examples, and case studies to enhance understanding. Key topics include plant-made pharmaceuticals, classical and non-classical techniques for secondary metabolite production in plant cell culture and their relevance to pharmaceutical science, edible vaccines, novel delivery systems for plant-based products, international industry regulatory guidelines, and more. Readers will find the book to be a comprehensive and valuable resource for the study of modern plant biotechnology approaches and their pharmaceutical applications. Builds upon the basic concepts of cell and plant tissue culture and recombinant DNA technology to better illustrate the modern and potential applications of plant biotechnology to the pharmaceutical sciences Provides detailed yet practical coverage of complex techniques, such as micropropogation, gene transfer, and biosynthesis Examines critical issues of international importance and offers real-life examples and potential solutions

Author : Jennie P. Mather,Penelope E. Roberts
Publisher : Springer Science & Business Media
Page : 247 pages
File Size : 42,6 Mb
Release : 2007-08-20
Category : Science
ISBN : 9780585275710

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Introduction to Cell and Tissue Culture by Jennie P. Mather,Penelope E. Roberts Pdf

It is a pleasure to contribute the foreword to Introduction to Cell and Tissue Culture: The ory and Techniques by Mather and Roberts. Despite the occasional appearance of thought ful works devoted to elementary or advanced cell culture methodology, a place remains for a comprehensive and definitive volume that can be used to advantage by both the novice and the expert in the field. In this book, Mather and Roberts present the relevant method ology within a conceptual framework of cell biology, genetics, nutrition, endocrinology, and physiology that renders technical cell culture information in a comprehensive, logical for mat. This allows topics to be presented with an emphasis on troubleshooting problems from a basis of understanding the underlying theory. The material is presented in a way that is adaptable to student use in formal courses; it also should be functional when used on a daily basis by professional cell culturists in a- demia and industry. The volume includes references to relevant Internet sites and other use ful sources of information. In addition to the fundamentals, attention is also given to mod ern applications and approaches to cell culture derivation, medium formulation, culture scale-up, and biotechnology, presented by scientists who are pioneers in these areas. With this volume, it should be possible to establish and maintain a cell culture laboratory devot ed to any of the many disciplines to which cell culture methodology is applicable.

Author : Robert H. Smith
Publisher : Academic Press
Page : 190 pages
File Size : 41,5 Mb
Release : 2012-12-02
Category : Science
ISBN : 9780323160476

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Plant Tissue Culture by Robert H. Smith Pdf

Plant Tissue Culture Techniques and Experiments is a manual that contains laboratory exercises about the demonstration of the methods and different plant materials used in plant tissue culture. It provides an overview on the plant cell culture techniques and plant material options in selecting the explant source. This book starts by discussing the proper setup of a tissue culture laboratory and the selection of the culture medium. It then explains the determination of an explant which is the ultimate goal of the cell culture project. The explant is a piece of plant tissue that is used in tissue culture. Furthermore, the book discusses topics about callus induction, regeneration and morphogenesis process, and haploid plants from anther and pollen culture. The meristem culture for virus-free plants and in vitro propagation for commercial propagation of ornamentals are also explained in this manual. The book also provides topics and exercises on the protoplast isolation and fusion and agrobacterium-mediated transformation of plants. This manual is intended for college students, both graduate and undergraduate, who study chemistry, plant anatomy, and plant physiology.

Author : Jenny Aitken-Christie,T. Kozai,M.A.L Smith
Publisher : Springer Science & Business Media
Page : 592 pages
File Size : 48,6 Mb
Release : 1995
Category : Juvenile Nonfiction
ISBN : 0792328418

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Automation and environmental control in plant tissue culture by Jenny Aitken-Christie,T. Kozai,M.A.L Smith Pdf

Automation in plant tissue culture; General introduction and overview; Economic analysis of automated micropropagation; Economic aspects of somati embryogenesis; Systems analysis and engineering; Engineering aspects of plant propagation in bioreactors; Mechanical engineering approaches to plant biotechnology; Image analysis for plant cell culture and micropropagation; Image analysis for embryogenesis; Automation of the bioreactor process for mass propagation and secondary metabolism; Delivery system for tissue culture by encapsulation; A delivery system for naked somatic embryos for interior spruce; Automated systems for organogenesis; Commercialisation of tissue culture and automated systems; Environmental control in plant tissue culture; General introduction; Physical microenvironmental adn its effects; Vessels, gels, liquid media, and support systems; The chemical mciroenvironment; Carbon nutrition in vitro; Regulation and manupulation of carbon assimilation in micropropagated systems; Ethylene; In vitro acclimatization; Low temperature storage of plant tissue cultures; Environmental measurement and control systems.

Author : J. Reinert,M.M. Yeoman
Publisher : Springer Science & Business Media
Page : 85 pages
File Size : 55,7 Mb
Release : 2012-12-06
Category : Science
ISBN : 9783642817847

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Plant Cell and Tissue Culture by J. Reinert,M.M. Yeoman Pdf

The techniques of plant organ, tissue, and cell culture concentrated on reproducibility, simplicity and accu are now established in many research laboratories racy with sufficient illustration to make all mani throughout the world and are being used in numerous pulations clear. areas of plant science. Methods have been developed The drawings of items used in the bench layout to propagate plants and free them from viruses using diagrams are symbolic and are 'keyed in' by number to shoot tip culture. The regeneration of plants from callus the list of materials and equipment. A line around an culture has also proved useful commercially. Elegant item indicates that is sterile. techniques have been used to synthesise somatic The adoption of an integrated text in which diagrams hybrids by the fusion of protoplasts and to transform are related spatially to the methods will, we hope, help cells. These and many other techniques have been the student to grasp the techniques quickly and effec and can be used to investigate a variety of botanical tively. This is first and foremost a manual which has its phenomena as well as to improve crop plants and now place on the laboratory bench open in front of the provide an important part of the basic experimental student, a book to be used! skills required by a majority of experimental botanists.

Author : Karl-Hermann Neumann,Ashwani Kumar,Jafargholi Imani
Publisher : Springer Nature
Page : 459 pages
File Size : 48,6 Mb
Release : 2020-10-01
Category : Science
ISBN : 9783030490980

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Plant Cell and Tissue Culture – A Tool in Biotechnology by Karl-Hermann Neumann,Ashwani Kumar,Jafargholi Imani Pdf

This textbook is clearly structured with fourteen richly illustrated chapters and practical examples for easy understanding and direct implementation. The methods and findings developed in the authors’ group are presented in detailed, revised chapters. Readers will find valuable updates on the molecular basis of biotechnological processes, secondary metabolite production and genetic engineering. In addition, the basic principles of important biotechnologies, as well as examples of specially designed crops that deliver improved productivity under stress conditions, are presented. This second edition sets the direction for future research on the basic aspects of plant tissue culture and its applications in the fields of secondary metabolite production and genetic engineering. It provides both general and specific information for students, teachers, academic researchers and industrial teams who are interested in new developments in plant tissue culture and its applications.