Plant Cell Tissue And Organ Culture Fundamental Methods
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Plant Cell, Tissue and Organ Culture by Oluf Gamborg,Gregory C. Phillips Pdf
This manual provides all relevant protocols for basic and applied plant cell and molecular technologies, such as histology, electron microscopy, cytology, virus diagnosis, gene transfer and PCR. Also included are chapters on laboratory facilities, operation and management as well as a glossary and all the information needed to set up and carry out any of the procedures without having to use other resource books. It is especially designed for professionals and advanced students who wish to acquire practical skills and first-hand experience in plant biotechnology.
Plant Cell, Tissue and Organ Culture by Anonim Pdf
A manual providing all relevant protocols for basic and applied plant cell and molecular technologies, such as histology, electron microscopy, cytology, virus diagnosis and gene transfer. Also included are chapters on laboratory facilities, operations and procedures.
Plant Cell, Tissue And Organ Culture: Fundamental Methods by Oluf L. Gamborg,Gregory C. Phillips Pdf
A manual providing all relevant protocols for basic and applied plant cell and molecular technologies, such as histology, electron microscopy, cytology, virus diagnosis and gene transfer. Also included are chapters on laboratory facilities, operations and procedures.
Applied and Fundamental Aspects of Plant Cell, Tissue, and Organ Culture by Jakob Reinert,Yashpal S. Bajaj Pdf
Progress in the field of plant cell and tissue culture has made this area of research one of the most dynamic and promising not only in plant physiology, cell biology and genetics but also in agriculture, forestry, horticulture and industry. Studies with plant cell cultures clearly have bearing upon a variety of problems as yet unsolved in basic and applied research. This was the compelling reason for assembling such a comprehensive source of information to stimulate students, teachers, and research workers. This book comprises 34 articles on regeneration of plants, vegetative propagation and cloning; haploids; cytology, cytogenetics and plant breeding; protoplasts, somatic hybridization and genetic engineering; plant pathology; secondary products and a chapter on isoenzymes, radiobiology, and cryobiology of plant cells. Particular attention has been paid to modern , fast-growing and fascinating disciplines - e.g. the induction of haploids, somatic hybridization and genetic manipulation by protoplast culture, which possess an enormous potential for plant improvement.
INTRODUCTION TO PLANT CELL TISSUE AND ORGAN CULTURE by SUNIL D. PUROHIT Pdf
Designed primarily as a text for undergraduate and postgraduate students of Botany and Plant Biotechnology, the book discusses the theoretical aspects and modern applications of plant cell, tissue and organ culture. Written with the aim of providing up-to-date information on the subject, and focused on the concept of commercialization of plant cell culture, the contents have been presented with clarity. The book not only discusses the theoretical aspects of plant tissue culture but also emphasizes the art of its practice. It also provides a systematic explanation of asepsis and methods of sterilization, plant tissue culture techniques, culture of reproductive structures, plant tissue culture in germplasm conservation, its applications in the industry and plant pathology and operation and management of greenhouse hardening unit. In addition, it discusses in vitro propagation of plants (micropropagation) with a series of case studies pertaining to tree species and horticultural crops. Besides students, the book will also prove to be useful for researchers, scholars and teachers.
Author : Michael R. Davey,Paul Anthony Publisher : John Wiley & Sons Page : 358 pages File Size : 46,9 Mb Release : 2010-05-20 Category : Science ISBN : 0470686510
Plant Cell Culture by Michael R. Davey,Paul Anthony Pdf
The ability to culture cells is fundamental for mass propagation and as a baseline for the genetic manipulation of plant nuclei and organelles. The introduction to Plant Cell Culture: Essential Methods provides a general background to plant cell culture, including basic principles, technologies and laboratory practices that underpin the more detailed techniques described in subsequent chapters. Whilst each chapter provides a background to the topic area and methodology, a crucial aspect is the provision of detailed protocols with emphasis on trouble shooting, describing common problems and detailed advice for their avoidance. Plant Cell Culture: Essential Methods provides the reader with a concise overview of these techniques, including micropropagation, mutagenesis, cryopreservation, genetic and plastid transformation and somatic cell technologies. This book will be an essential addition to any plant science laboratory's bookshelf. Highlights the best and most up-to-date techniques for working on plant cell culture Explains clearly and precisely how to carry out selected techniques in addition to background information on the various approaches Chapters are written by leading international authorities in the field and cover both well-known and new, tried and tested, methods for working in plant cell culture An essential laboratory manual for students and early-career researchers.
Author : NIIR Board of Consultants and Engineers Publisher : ASIA PACIFIC BUSINESS PRESS Inc. Page : 605 pages File Size : 45,8 Mb Release : 2005-10-01 Category : Business & Economics ISBN : 9788178330297
Handbook on Plant and Cell Tissue Culture by NIIR Board of Consultants and Engineers Pdf
Plants cell tissue culture is a rapidly developing technology which holds promise of restructuring agricultural and forestry practices. During the last two decades cell culture have made considerable advanced in the field of agriculture, horticulture, plant breeding, forestry, somatic cell genetics, phytopathology etc. Plant cells can be grown in isolation from intact plants in tissue culture systems. The cells have the characteristics of callus cells, rather than other plant cell types. These are the cells that appear on cut surfaces when a plant is wounded and which gradually cover and seal the damaged area. Plant cells and tissue culture are often used for the production of primary and secondary metabolites. Plant tissue cultures can be initiated from almost any part of a plant. The physiological state of the plant does have an influence on its response to attempts to initiate tissue culture. The parent plant must be healthy and free from obvious signs of disease or decay. The source, termed explant, may be dictated by the reason for carrying out the tissue culture. Younger tissue contains a higher proportion of actively dividing cells and is more responsive to a callus initiation programme. The plants themselves must be actively growing, and not about to enter a period of dormancy. Plant tissue culture is used widely in plant science; it also has a number of commercial applications. Tissue culture is employed in; micropropagation, elimination of pathogens from plant materials, germoplasm storage, production of somaclonal varients, embryo rescue, production of haploids, production of artificial seeds, production of secondary metabolities, production of transgenic plants etc. Some of the fundamentals of the book are plant tissue culture, basic requirements for tissue culture laboratory, surface sterilization of explant materials, development of tissue culture techniques, principles of cell culture cell, special factors influencing growth and metabolism, media for culturing cells and tissues, sterilisation procedures, design and equipment of a tissue culture laboratory, isolation method for microorganisms for culture, culture preservation and stability, genetic modification of industrial microorganisms mutation etc. The present book discuss about the methods, culture preservation and stability procedures, storage and transportation of plant cell tissue culture. This book is an invaluable resource for research workers, students, technocrats, entrepreneurs, institutional libraries etc. TAGS Plant Tissue Culture in India, Commercialization of Plant Tissue Culture in India, Role of Plant Tissue Culture in Agriculture, Plant Tissue Culture Industry in India, Industrial Plant Tissue Culture, Tissue Culture in Agriculture, Plant Tissue Culture, Tissue Culture, Cell Culture and Tissue Culture, Tissue Culture and Cell Culture, Tissue Culture in Plants, Plant Cell and Tissue Culture, Commercial Plant Tissue Culture in India, Plant Tissue Culture Business Plan, Plant Tissue Culture and Biotechnology, Tissue Culture Plants, Plant Tissue Culture Business Plan, Business Opportunities in Plant Tissue Culture, Tissue Culture Methods, Cybrid Production, Process of Cybrids Production, Production of Cybrids, Production of Cybrid Plants, Production of Haploid Plants, Haploid Production, Plant Secondary Metabolism, Production of Secondary Metabolites, Production of Secondary Metabolites Using Plant Cell Cultures, Plant Tissue Cultures in Production of Secondary Metabolites, Secondary Metabolites Production, Production of Somatic Hybrid Plants, Somatic Hybridization of Plants, Somatic Hybrid, Somatic Hybrid Production, Production of Enriched Biomass, Enrichment on Biomass Production, Formulation of Tissue Culture Medium, Collection of Explant Materials, Subculture of Callus, Regeneration of Plants from Callus, Preparation of Chick Embryo Extract, Preparation of Embryo Extract from Young Embryos, Preparation of Bovine Embryo Extract, Preparation of Eagles Medium, Media for Plant Tissues, Organ Culture, Preparation of Trypsinised Embryonic Carcass, Enrichment Culture Methods, Genetic Modification of Industrial Microorganisms Mutation, Methods Favouring Formation of Hybrid DNA Molecules, Modes of Growth of Bacteria and Fungi, Mixed Culture and Mixed Substrate Systems, Spontaneous Mixed Culture Process, Maintenance of Protoplasts, Collection of Plant Materials, Storage of Germ Plasm of Potato, Mammalian Embryonic Tissues, Preparation of Tissues from Plants, Largescale Culture Methods, Preparation and Sterilisation of Apparatus, Preparation and Sterilisation of Media, Reservation, Storage and Transportation of Living Tissues and Cells, Culture of Plant Cells for Extraction of Secondary Metabolites, Preparation of Explant, Suspension Culture, Extraction of Secondary Metabolites, Biotransformation in Plant Cells, Immobilization of Plant Cells, Special Tissue Culture Media, Manufacturing Plant Cultures, Products from Plant Tissue Culture, Cultivation of Plant Tissue, Cultures of Tomato Roots, Tissue Culture of Tomato Roots, Preparation of Carrot Callus Culture, Tissue Culture of Carrot Callus, Carrot Callus Tissue for Culture, Cultivation of Cells in Vivo Transplantation, Cultures on Agar, Npcs, Niir, Process Technology Books, Business Consultancy, Business Consultant, Project Identification and Selection, Preparation of Project Profiles, Startup, Business Guidance, Business Guidance to Clients, Startup Project, Startup Ideas, Project for Startups, Startup Project Plan, Business Start-Up, Business Plan for Startup Business, Great Opportunity for Startup, Small Start-Up Business Project, Best Small and Cottage Scale Industries, Startup India, Stand Up India, Small Scale Industries, New Small Scale Ideas for Haploid Production Industry, Cybrid Production Business Ideas You Can Start on Your Own, Indian Secondary Metabolites Production Industry, Small Scale Somatic Hybrid Production, Guide to Starting and Operating Small Business, Business Ideas for Enriched Biomass Production, How to Start Secondary Metabolites Production Business, Starting Enriched Biomass Production, Start Your Own Somatic Hybrid Production Business, Secondary Metabolites Production Business Plan, Business Plan for Cybrid Production, Small Scale Industries in India, Haploid Production Based Small Business Ideas in India, Small Scale Industry You Can Start on Your Own, Business Plan for Small Scale Industries, Set Up Cybrid Production, Profitable Small Scale Manufacturing, How to Start Small Business in India, Free Manufacturing Business Plans, Small and Medium Scale Manufacturing, Profitable Small Business Industries Ideas, Business Ideas for Startup
Plant Cell and Tissue Culture – A Tool in Biotechnology by Karl-Hermann Neumann,Ashwani Kumar,Jafargholi Imani Pdf
This textbook is clearly structured with fourteen richly illustrated chapters and practical examples for easy understanding and direct implementation. The methods and findings developed in the authors’ group are presented in detailed, revised chapters. Readers will find valuable updates on the molecular basis of biotechnological processes, secondary metabolite production and genetic engineering. In addition, the basic principles of important biotechnologies, as well as examples of specially designed crops that deliver improved productivity under stress conditions, are presented. This second edition sets the direction for future research on the basic aspects of plant tissue culture and its applications in the fields of secondary metabolite production and genetic engineering. It provides both general and specific information for students, teachers, academic researchers and industrial teams who are interested in new developments in plant tissue culture and its applications.
Plant Tissue Culture: Techniques and Experiments, Fourth Edition, builds on the classroom tested, audience proven manual that has guided users through successful plant culturing for almost 30 years. The book's experiments demonstrate major concepts and can be conducted with a variety of plant materials readily available throughout the year. This fully updated edition describes the principles of the newest technologies, including CRISPR/Cas9 gene editing and RNAi technology with plant cell and tissue cultures and their applications. Bridging the gap between theory and practice, this book contains detailed methodology supported by comprehensive illustrations, giving users a diverse learning experience for both university students and plant scientists. Provides fundamental principles, methods and techniques in plant cell, tissue and organ culture that can be applied to all crop plants, including agronomic crops, horticulture and forestry crops for germplasm improvement Guides readers from lab setup to supplies, stock solution and media preparation, explant selection and disinfestations, and experimental observations and measurement Contains the latest advances and updates since the previous edition published in 2012
Plant Cell and Tissue Culture by Indra K. Vasil,Trevor A. Thorpe Pdf
Plant Cell and Tissue Culture gives an exhaustive account of plant cell culture and genetic transformation, including detailed chapters on all major field and plantation crops. Part A presents a comprehensive coverage of all necessary laboratory techniques for the initiation, nutrition, maintenance and storage of plant cell and tissue cultures, including discussions on these topics, as well as on morphogenesis and regeneration, meristem and shoot tip culture, plant protoplasts, mutant cell lines, variation in tissue cultures, isogenic lines, fertilization control, cryopreservation, transformation, and the production of secondary metabolites. Part B then proceeds into detail on the specific in vitro culture of specific crops, including cereals, legumes, vegetables, potatoes, other roots and tubers, oilseeds, temperate fruits, tropical fruits, plantation crops, forest trees and ornamentals. Plant Cell and Tissue Culture is, and is likely to remain, the laboratory manual of choice, as well as a source of inspiration and a guide to all workers in the field.
Plant Tissue Culture Concepts and Laboratory Exercises by Robert N. Trigiano Pdf
Alternating between topic discussions and hands-on laboratory experiments that range from the in vitro flowering of roses to tissue culture of ferns, Plant Tissue Culture Concepts and Laboratory Exercises, Second Edition, addresses the most current principles and methods in plant tissue culture research. The editors use the expertise of some of the top researchers and educators in plant biotechnology to furnish students, instructors and researchers with a broad consideration of the field. Divided into eight major parts, the text covers everything from the history of plant tissue culture and basic methods to propagation techniques, crop improvement procedures, specialized applications and nutrition of callus cultures. New topic discussions and laboratory exercises in the Second Edition include ""Micropropagation of Dieffenbachia,"" ""Micropropagation and in vitro flowering of rose,"" ""Propagation from nonmeristematic tissue-organogenesis,"" ""Variation in culture"" and ""Tissue culture of ferns.""It is the book's extensive laboratory exercises that provide a hands-on approach in illustrating various topics of discussion, featuring step-by-step procedures, anticipated results, and a list of materials needed. What's more, editors Trigiano and Gray go beyond mere basic principles of plant tissue culture by including chapters on genetic transformation techniques, and photographic methods and statistical analysis of data. In all, Plant Tissue Culture Concepts and Laboratory Exercises, Second Edition, is a veritable harvest of information for the continued study and research in plant tissue culture science.
Author : John H. Dodds,Lorin W. Roberts Publisher : International Potato Center Page : 260 pages File Size : 50,5 Mb Release : 1985-10-31 Category : Science ISBN : 0521315166
Experiments in Plant Tissue Culture by John H. Dodds,Lorin W. Roberts Pdf
The second edition of Experiments in Plant Tissue Culture makes available new information that has resulted from recent advances in the applications of plant tissue culture techniques to agriculture and industry. This comprehensive laboratory text takes the reader through a graded series of experimental protocols and also provides an introductory review of each topic. Topics include: a plant tissue culture laboratory, aseptic techniques, nutritional components of media, callus induction, organ formation, xylem cell differentiation, root cultures, cell suspensions, micropropagation, embryogenesis, isolation and fusion of protoplasts, haploid cultures, storage of plant genetic resources, secondary metabolite production, and quantification of procedures. This volume offers all of the basic experimental methods for the major research areas of plant tissue culture, and it will be invaluable to undergraduates and research investigators in the plant sciences.
