Introduction To Quantitative Cell Biology

Author : Wallace F. Marshall
Publisher : Biota Publishing
Page : 60 pages
File Size : 45,9 Mb
Release : 2017-07-20
Category : Science
ISBN : 9781615046690

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Introduction to Quantitative Cell Biology by Wallace F. Marshall Pdf

For the past decade or more, much of cell biology research has been focused on determining the key molecules involved in different cellular processes, an analytical problem that has been amenable to biochemical and genetic approaches. Now, we face an integrative problem of understanding how all of these molecules work together to produce living cells, a challenge that requires using quantitative approaches to model the complex interactions within a cell, and testing those models with careful quantitative measurements. This book is an introductory overview of the various approaches, methods, techniques, and models employed in quantitative cell biology, which are reviewed in greater detail in the other volumes in this e-book series. Particular emphasis is placed on the goals and purpose of quantitative analysis and modeling, and the special challenges that cell biology holds for understanding life at the physical level.

Author : Akatsuki Kimura
Publisher : Unknown
Page : 0 pages
File Size : 40,5 Mb
Release : 2022
Category : Electronic
ISBN : 9811650195

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Quantitative Biology by Akatsuki Kimura Pdf

This textbook is for biologists, to conduct quantitative analysis and modeling of biological processes at molecular and cellular levels. Focusing on practical concepts and techniques for everyday research, this text will enable beginners, both students and established biologists, to take the first step in quantitative biology. It also provides step-by-step tutorials to run various sample programs in one's personal computer using Excel and Python. This volume traces topics, starting with an introductory chapter, such as modeling, construction and execution of numerical models, and key concepts in quantitative biology: feedback regulations, fluctuations and randomness, and statistical analyses. It also provide sample codes with guidance to procedure programming for actual biological processes such as movement of the nucleus within a cell, cell-cycle regulation, stripe pattern formation of skins, and distribution of energy. Written by a leading research scientist who has a background in biology, studied quantitative approaches by himself, and teaches quantitative biology at several universities, this textbook broadens quantitative approaches for biologists who do not have a strong background in mathematics, physics, or computer programming, and helps them progress further in their research.

Author : Ron Milo,Rob Phillips
Publisher : Garland Science
Page : 400 pages
File Size : 41,6 Mb
Release : 2015-12-07
Category : Science
ISBN : 9781317230694

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Cell Biology by the Numbers by Ron Milo,Rob Phillips Pdf

A Top 25 CHOICE 2016 Title, and recipient of the CHOICE Outstanding Academic Title (OAT) Award. How much energy is released in ATP hydrolysis? How many mRNAs are in a cell? How genetically similar are two random people? What is faster, transcription or translation?Cell Biology by the Numbers explores these questions and dozens of others provid

Author : Ferdinand Heinmets
Publisher : Unknown
Page : 358 pages
File Size : 42,5 Mb
Release : 1970
Category : Science
ISBN : WISC:89031209463

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Quantitative Cellular Biology by Ferdinand Heinmets Pdf

Author : Brian P. Ingalls
Publisher : MIT Press
Page : 423 pages
File Size : 42,7 Mb
Release : 2022-06-07
Category : Science
ISBN : 9780262545822

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Mathematical Modeling in Systems Biology by Brian P. Ingalls Pdf

An introduction to the mathematical concepts and techniques needed for the construction and analysis of models in molecular systems biology. Systems techniques are integral to current research in molecular cell biology, and system-level investigations are often accompanied by mathematical models. These models serve as working hypotheses: they help us to understand and predict the behavior of complex systems. This book offers an introduction to mathematical concepts and techniques needed for the construction and interpretation of models in molecular systems biology. It is accessible to upper-level undergraduate or graduate students in life science or engineering who have some familiarity with calculus, and will be a useful reference for researchers at all levels. The first four chapters cover the basics of mathematical modeling in molecular systems biology. The last four chapters address specific biological domains, treating modeling of metabolic networks, of signal transduction pathways, of gene regulatory networks, and of electrophysiology and neuronal action potentials. Chapters 3–8 end with optional sections that address more specialized modeling topics. Exercises, solvable with pen-and-paper calculations, appear throughout the text to encourage interaction with the mathematical techniques. More involved end-of-chapter problem sets require computational software. Appendixes provide a review of basic concepts of molecular biology, additional mathematical background material, and tutorials for two computational software packages (XPPAUT and MATLAB) that can be used for model simulation and analysis.

Author : Anonim
Publisher : Academic Press
Page : 588 pages
File Size : 47,7 Mb
Release : 2014-06-25
Category : Science
ISBN : 9780124202016

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Quantitative Imaging in Cell Biology by Anonim Pdf

This new volume, number 123, of Methods in Cell Biology looks at methods for quantitative imaging in cell biology. It covers both theoretical and practical aspects of using optical fluorescence microscopy and image analysis techniques for quantitative applications. The introductory chapters cover fundamental concepts and techniques important for obtaining accurate and precise quantitative data from imaging systems. These chapters address how choice of microscope, fluorophores, and digital detector impact the quality of quantitative data, and include step-by-step protocols for capturing and analyzing quantitative images. Common quantitative applications, including co-localization, ratiometric imaging, and counting molecules, are covered in detail. Practical chapters cover topics critical to getting the most out of your imaging system, from microscope maintenance to creating standardized samples for measuring resolution. Later chapters cover recent advances in quantitative imaging techniques, including super-resolution and light sheet microscopy. With cutting-edge material, this comprehensive collection is intended to guide researchers for years to come. Covers sections on model systems and functional studies, imaging-based approaches and emerging studies Chapters are written by experts in the field Cutting-edge material

Author : Michael E. Wall
Publisher : CRC Press
Page : 392 pages
File Size : 53,9 Mb
Release : 2012-08-25
Category : Science
ISBN : 9781439827239

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Quantitative Biology by Michael E. Wall Pdf

Quantitative methods are revolutionizing modern molecular and cellular biology. Groundbreaking technical advances are fueling the rapid expansion in our ability to observe, as seen in multidisciplinary studies that integrate theory, computation, experimental assays, and the control of microenvironments. Integrating new experimental and theoretical

Author : Heinments F.
Publisher : Unknown
Page : 128 pages
File Size : 44,5 Mb
Release : 1974
Category : Electronic
ISBN : OCLC:769809926

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Quantitative Cellular Biology by Heinments F. Pdf

Author : Philip N. Benfey
Publisher : Unknown
Page : 0 pages
File Size : 50,8 Mb
Release : 2014
Category : SCIENCE
ISBN : 1621820343

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Quickstart Molecular Biology by Philip N. Benfey Pdf

"This book is an introductory course in molecular biology for mathematicians, physicists, and engineers. It covers the basic features of DNA, proteins, and cells but in the context of recent technological advances, such as next-generation sequencing and high-throughput screens, and their applications. This enables readers to move rapidly from the b

Author : Valerie Coffman,I-Ju Lee,Jian-Qiu Wu
Publisher : Biota Publishing
Page : 76 pages
File Size : 49,6 Mb
Release : 2014-10-01
Category : Science
ISBN : 9781615046553

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Counting Molecules Within Cells by Valerie Coffman,I-Ju Lee,Jian-Qiu Wu Pdf

A fundamental component of quantitative cell biology is the ability to count molecules within cells. The numbers of molecules and stoichiometries are the basis for structural models of protein complexes and simulations of biological processes. A variety of methods exist for in vivo quantifications, but the focus of this volume is mainly on fluorescence methods. The two most popular methods are stepwise photobleaching and ratio comparison using a standard curve. With recent advances in genome editing techniques, most model organisms are amenable to inserting coding sequences for fluorescent proteins into native genetic loci, making quantification of proteins by fluorescence microscopy one of the most ubiquitous tools available to cell biologists. The acquisition and analysis methods range from simple to complex, and most have been validated by counting with multiple methods and other types of data. Researchers should be aware of sources of error in the acquisition and analysis, but the accuracy of these methods is high. Quantification by fluorescence microscopy has yielded valuable new insights into many aspects of cell biology, highlighting its place among the standard tools for molecular and cell biologists.

Author : Julien Berro,Michael M. Lacy
Publisher : Biota Publishing
Page : 88 pages
File Size : 51,6 Mb
Release : 2018-07-25
Category : Science
ISBN : 9781615047857

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Quantitative Biology of Endocytosis by Julien Berro,Michael M. Lacy Pdf

Clathrin-mediated endocytosis (CME) is a ubiquitous internalization process in eukaryotic cells. It consists of the formation of an approximately 50-nm diameter vesicle out of a flat membrane. Genetics, biochemistry, and microscopy experiments performed in the last four decades have been instrumental to discover and characterize major endocytic proteins in yeast and mammals. However, due to the highly dynamic nature of the endocytic assembly and its small size, many questions remain unresolved: how are endocytic proteins organized spatially and dynamically? How are forces produced and how are their directions controlled? How do the biochemical activities of endocytic proteins and the membrane shape and mechanics regulate each other? These questions are virtually impossible to visualize or measure directly with conventional approaches but thanks to new quantitative biology methods, it is now possible to infer the mechanisms of endocytosis in exquisite detail. This book introduces quantitative microscopy and mathematical modeling approaches that have been used to count the copy number of endocytic proteins, infer their localization with nanometer precision, and infer molecular and physical mechanisms that are involved in the robust formation of endocytic vesicles.

Author : Joseph J Feher
Publisher : Academic Press
Page : 1008 pages
File Size : 53,8 Mb
Release : 2017-01-02
Category : Medical
ISBN : 9780128011546

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Quantitative Human Physiology by Joseph J Feher Pdf

Quantitative Human Physiology: An Introduction is the first text to meet the needs of the undergraduate bioengineering student who is being exposed to physiology for the first time, but requires a more analytical/quantitative approach. This book explores how component behavior produces system behavior in physiological systems. Through text explanation, figures, and equations, it provides the engineering student with a basic understanding of physiological principles with an emphasis on quantitative aspects. Features a quantitative approach that includes physical and chemical principles Provides a more integrated approach from first principles, integrating anatomy, molecular biology, biochemistry and physiology Includes clinical applications relevant to the biomedical engineering student (TENS, cochlear implants, blood substitutes, etc.) Integrates labs and problem sets to provide opportunities for practice and assessment throughout the course NEW FOR THE SECOND EDITION Expansion of many sections to include relevant information Addition of many new figures and re-drawing of other figures to update our understanding and clarify difficult areas Substantial updating of the text to reflect newer research results Addition of several new appendices including statistics, nomenclature of transport carriers, and structural biology of important items such as the neuromuscular junction and calcium release unit Addition of new problems within the problem sets Addition of commentary to power point presentations

Author : Raimund J. Ober,E. Sally Ward,Jerry Chao
Publisher : CRC Press
Page : 552 pages
File Size : 40,8 Mb
Release : 2020-12-15
Category : Medical
ISBN : 9780429892134

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Quantitative Bioimaging by Raimund J. Ober,E. Sally Ward,Jerry Chao Pdf

Quantitative bioimaging is a broad interdisciplinary field that exploits tools from biology, chemistry, optics, and statistical data analysis for the design and implementation of investigations of biological processes. Instead of adopting the traditional approach of focusing on just one of the component disciplines, this textbook provides a unique introduction to quantitative bioimaging that presents all of the disciplines in an integrated manner. The wide range of topics covered include basic concepts in molecular and cellular biology, relevant aspects of antibody technology, instrumentation and experimental design in fluorescence microscopy, introductory geometrical optics and diffraction theory, and parameter estimation and information theory for the analysis of stochastic data. Key Features: Comprises four parts, the first of which provides an overview of the topics that are developed from fundamental principles to more advanced levels in the other parts. Presents in the second part an in-depth introduction to the relevant background in molecular and cellular biology and in physical chemistry, which should be particularly useful for students without a formal background in these subjects. Provides in the third part a detailed treatment of microscopy techniques and optics, again starting from basic principles. Introduces in the fourth part modern statistical approaches to the determination of parameters of interest from microscopy data, in particular data generated by single molecule microscopy experiments. Uses two topics related to protein trafficking (transferrin trafficking and FcRn-mediated antibody trafficking) throughout the text to motivate and illustrate microscopy techniques. An online appendix providing the background and derivations for various mathematical results presented or used in the text is available at http://www.routledge.com/9781138598980.

Author : James Ferrell
Publisher : Garland Science
Page : 285 pages
File Size : 54,5 Mb
Release : 2021-09-28
Category : Science
ISBN : 9781000430738

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Systems Biology of Cell Signaling by James Ferrell Pdf

How can we understand the complexity of genes, RNAs, and proteins and the associated regulatory networks? One approach is to look for recurring types of dynamical behavior. Mathematical models prove to be useful, especially models coming from theories of biochemical reactions such as ordinary differential equation models. Clever, careful experiments test these models and their basis in specific theories. This textbook aims to provide advanced students with the tools and insights needed to carry out studies of signal transduction drawing on modeling, theory, and experimentation. Early chapters summarize the basic building blocks of signaling systems: binding/dissociation, synthesis/destruction, and activation/inactivation. Subsequent chapters introduce various basic circuit devices: amplifiers, stabilizers, pulse generators, switches, stochastic spike generators, and oscillators. All chapters consistently use approaches and concepts from chemical kinetics and nonlinear dynamics, including rate-balance analysis, phase plane analysis, nullclines, linear stability analysis, stable nodes, saddles, unstable nodes, stable and unstable spirals, and bifurcations. This textbook seeks to provide quantitatively inclined biologists and biologically inclined physicists with the tools and insights needed to apply modeling and theory to interesting biological processes. Key Features: Full-color illustration program with diagrams to help illuminate the concepts Enables the reader to apply modeling and theory to the biological processes Further Reading for each chapter High-quality figures available for instructors to download

Author : Margaret Gardel,Patrick Oakes
Publisher : Biota Publishing
Page : 77 pages
File Size : 48,9 Mb
Release : 2015-09-01
Category : Science
ISBN : 9781615046997

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Measuring Cell Mechanics by Margaret Gardel,Patrick Oakes Pdf

Cells are inherently physical entities that both experience mechanical forces from their external environment and generate their own internal forces to drive cell motion. Our particular aim here is to present the reader with an introduction to the primary tools used to measure these mechanical interactions and the material properties of cells that result from them. These approaches can be applied to a diverse array of physiological processes and systems, providing important insight into the regulatory roles of mechanical interactions in cells. We cover techniques at both the molecular and cellular scales, including those that actively and passively probe the system. Along the way we cover the fundamental principles of each approach, while emphasizing the relevant length and timescales, along with the typical magnitudes of the measurements that can be made. Each section ends by highlighting uses of the various techniques in recent relevant publications, illustrating the exciting future of cell mechanics in quantitative cell biology research.