Quantitative Imaging In Cell Biology

Author : Anonim
Publisher : Academic Press
Page : 588 pages
File Size : 45,5 Mb
Release : 2014-06-25
Category : Science
ISBN : 9780124202016

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Quantitative Imaging in Cell Biology by Anonim Pdf

This new volume, number 123, of Methods in Cell Biology looks at methods for quantitative imaging in cell biology. It covers both theoretical and practical aspects of using optical fluorescence microscopy and image analysis techniques for quantitative applications. The introductory chapters cover fundamental concepts and techniques important for obtaining accurate and precise quantitative data from imaging systems. These chapters address how choice of microscope, fluorophores, and digital detector impact the quality of quantitative data, and include step-by-step protocols for capturing and analyzing quantitative images. Common quantitative applications, including co-localization, ratiometric imaging, and counting molecules, are covered in detail. Practical chapters cover topics critical to getting the most out of your imaging system, from microscope maintenance to creating standardized samples for measuring resolution. Later chapters cover recent advances in quantitative imaging techniques, including super-resolution and light sheet microscopy. With cutting-edge material, this comprehensive collection is intended to guide researchers for years to come. Covers sections on model systems and functional studies, imaging-based approaches and emerging studies Chapters are written by experts in the field Cutting-edge material

Author : Anonim
Publisher : Academic Press
Page : 502 pages
File Size : 52,5 Mb
Release : 1989-04-01
Category : Science
ISBN : 0080859283

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Fluorescence Microscopy of Living Cells in Culture, Part B by Anonim Pdf

Fluorescence Microscopy of Living Cells in Culture, Part B

Author : Ruslan I. Dmitriev
Publisher : Springer
Page : 172 pages
File Size : 49,7 Mb
Release : 2017-10-26
Category : Science
ISBN : 9783319673585

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Multi-Parametric Live Cell Microscopy of 3D Tissue Models by Ruslan I. Dmitriev Pdf

This book provides an essential overview of existing state-of-the-art quantitative imaging methodologies and protocols (intensity-based ratiometric and FLIM/ PLIM). A variety of applications are covered, including multi-parametric quantitative imaging in intestinal organoid culture, autofluorescence imaging in cancer and stem cell biology, Ca2+ imaging in neural ex vivo tissue models, as well as multi-parametric imaging of pH and viscosity in cancer biology. The current state-of-the-art of 3D tissue models and their compatibility with live cell imaging is also covered. This is an ideal book for specialists working in tissue engineering and designing novel biomaterial.

Author : JENS. RITTSCHER
Publisher : Unknown
Page : 128 pages
File Size : 41,7 Mb
Release : 2019
Category : Electronic
ISBN : 1138627658

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QUANTITATIVE IMAGING by JENS. RITTSCHER Pdf

Author : Greenfield Sluder,David E. Wolf
Publisher : Elsevier
Page : 632 pages
File Size : 42,8 Mb
Release : 2007-04-26
Category : Science
ISBN : 0080544347

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Digital Microscopy by Greenfield Sluder,David E. Wolf Pdf

The previous edition of this book marked the shift in technology from video to digital camera use with microscope use in biological science. This new edition presents some of the optical fundamentals needed to provide a quality image to the digital camera. Specifically, it covers the fundamental geometric optics of finite- and infinity-corrected microscopes, develops the concepts of physical optics and Abbe’s theory of image formation, presents the principles of Kohler illumination, and finally reviews the fundamentals of fluorescence and fluorescence microscopy. The second group of chapters deals with digital and video fundamentals: how digital and video cameras work, how to coordinate cameras with microscopes, how to deal with digital data, the fundamentals of image processing, and low light level cameras. The third group of chapters address some specialized areas of microscopy that allow sophisticated measurements of events in living cells that are below the optical limits of resolution. * Expands coverage to include discussion of confocal microscopy not found in the previous edition * Includes "traps and pitfalls" as well as laboratory exercises to help illustrate methods

Author : Spencer L. Shorte,Friedrich Frischknecht
Publisher : Springer Science & Business Media
Page : 450 pages
File Size : 54,8 Mb
Release : 2007-09-12
Category : Science
ISBN : 9783540713319

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Imaging Cellular and Molecular Biological Functions by Spencer L. Shorte,Friedrich Frischknecht Pdf

This book offers a comprehensive selection of essays by leading experts, which covers all aspects of modern imaging, from its application and up-scaling to its development. The chapter content ranges from the basics to the most complex overview of method and protocols. There is ample practical and detailed "how-to" content on important, but rarely addressed topics. This first edition features all-colour-plate chapters, licensed software and a unique, continuously updated website forum.

Author : Douglas J. Taatjes,Brooke T. Mossman
Publisher : Springer Science & Business Media
Page : 505 pages
File Size : 49,6 Mb
Release : 2008-02-04
Category : Medical
ISBN : 9781592599936

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Cell Imaging Techniques by Douglas J. Taatjes,Brooke T. Mossman Pdf

A diverse collection of state-of-the-art methods for the microscopic imaging of cells and molecules. The authors cover a wide spectrum of complimentary techniques, including such methods as fluorescence microscopy, electron microscopy, atomic force microscopy, and laser scanning cytometry. Additional readily reproducible protocols on confocal scanning laser microscopy, quantitative computer-assisted image analysis, laser-capture microdissection, microarray image scanning, near-field scanning optical microscopy, and reflection contrast microscopy round out this eclectic collection of cutting-edge imaging techniques now available. The authors also discuss preparative methods for particles and cells by transmission electron microscopy.

Author : Raimund J. Ober,E. Sally Ward,Jerry Chao
Publisher : CRC Press
Page : 693 pages
File Size : 55,8 Mb
Release : 2020-12-15
Category : Medical
ISBN : 9780429892127

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Quantitative Bioimaging by Raimund J. Ober,E. Sally Ward,Jerry Chao Pdf

Quantitative bioimaging is a broad interdisciplinary field that exploits tools from biology, chemistry, optics, and statistical data analysis for the design and implementation of investigations of biological processes. Instead of adopting the traditional approach of focusing on just one of the component disciplines, this textbook provides a unique introduction to quantitative bioimaging that presents all of the disciplines in an integrated manner. The wide range of topics covered include basic concepts in molecular and cellular biology, relevant aspects of antibody technology, instrumentation and experimental design in fluorescence microscopy, introductory geometrical optics and diffraction theory, and parameter estimation and information theory for the analysis of stochastic data. Key Features: Comprises four parts, the first of which provides an overview of the topics that are developed from fundamental principles to more advanced levels in the other parts. Presents in the second part an in-depth introduction to the relevant background in molecular and cellular biology and in physical chemistry, which should be particularly useful for students without a formal background in these subjects. Provides in the third part a detailed treatment of microscopy techniques and optics, again starting from basic principles. Introduces in the fourth part modern statistical approaches to the determination of parameters of interest from microscopy data, in particular data generated by single molecule microscopy experiments. Uses two topics related to protein trafficking (transferrin trafficking and FcRn-mediated antibody trafficking) throughout the text to motivate and illustrate microscopy techniques. An online appendix providing the background and derivations for various mathematical results presented or used in the text is available at http://www.routledge.com/9781138598980.

Author : Natasha S. Barteneva,Ivan A. Vorobjev
Publisher : Humana
Page : 0 pages
File Size : 45,5 Mb
Release : 2015-11-23
Category : Medical
ISBN : 1493933000

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Imaging Flow Cytometry by Natasha S. Barteneva,Ivan A. Vorobjev Pdf

This detailed volume for the first time explores techniques and protocols involving quantitative imaging flow cytometry (IFC), which has revolutionized our ability to analyze cells, cellular clusters, and populations in a remarkable fashion. Beginning with an introduction to technology, the book continues with sections addressing protocols for studies on the cell nucleus, nucleic acids, and FISH techniques using an IFC instrument, immune response analysis and drug screening, IFC protocols for apoptosis and cell death analysis, as well as morphological analysis and the identification of rare cells. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Imaging Flow Cytometry: Methods and Protocols will be a critical source for all laboratories seeking to implement IFC in their research studies.

Author : F. W. D. Rost
Publisher : Cambridge University Press
Page : 264 pages
File Size : 42,6 Mb
Release : 1991-07-26
Category : Science
ISBN : 0521394228

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Quantitative Fluorescence Microscopy by F. W. D. Rost Pdf

Quantitative fluorescence microscopy is concerned with making measurements from fluorescent specimens in a fluorescence microscope, by measuring fluorescence emission from a defined area or areas of a specimen. This technique is most commonly used to determine the amount of some specific substance, such as DNA, in some particular area of a cell. But it has many other uses; for example, it can be used to identify certain substances in the cell by examining their fluorescence characteristics. This book is a complete guide to this technique for all biologists. It describes the principles and applications of quantitative fluorescence microscopy and also gives much practical information about the instrumentation required. There is also a discussion of the exciting developments in confocal fluorescence microscopy which allows the three dimensional distribution of particular substances to be determined. Everyone presently using this technique, or wishing to start using it will need to read this book.

Author : Guy Cox
Publisher : CRC Press
Page : 319 pages
File Size : 48,8 Mb
Release : 2012-06-04
Category : Medical
ISBN : 9781439848258

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Optical Imaging Techniques in Cell Biology, Second Edition by Guy Cox Pdf

Optical Imaging Techniques in Cell Biology, Second Edition covers the field of biological microscopy, from the optics of the microscope to the latest advances in imaging below the traditional resolution limit. It includes the techniques—such as labeling by immunofluorescence and fluorescent proteins—which have revolutionized cell biology. Quantitative techniques such as lifetime imaging, ratiometric measurement, and photoconversion are all covered in detail. Expanded with a new chapter and 40 new figures, the second edition has been updated to cover the latest developments in optical imaging techniques. Explanations throughout are accurate, detailed, but as far as possible non-mathematical. This edition includes appendices with useful practical protocols, references, and suggestions for further reading. Color figures are integrated throughout.

Author : Sung-Bae Kim
Publisher : Humana
Page : 447 pages
File Size : 44,7 Mb
Release : 2022-06-12
Category : Science
ISBN : 1071612603

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Live Cell Imaging by Sung-Bae Kim Pdf

This detailed book highlights recent advances in molecular imaging techniques and protocols, designed to be immediately applicable in global bio-laboratories. The chapters are categorized into seven major groups according to the reporter materials, such as imaging with passive optical readouts, activatable bioluminescent probes, functional substrates and luciferases, organic fluorescent probes, BRET probes, FRET probes, as well as with advanced instrumentation. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Live Cell Imaging: Methods and Protocols aims to direct and inspire researchers into creating smarter, next-generation imaging techniques that are truly quantitative, highly sensitive, and readily comprehended, in the effort to engender deeper understanding of biological systems and break new ground in the research fields of life science.

Author : Anda Cornea,P. Michael Conn
Publisher : Elsevier
Page : 260 pages
File Size : 44,6 Mb
Release : 2014-02-24
Category : Science
ISBN : 9780124167131

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Fluorescence Microscopy by Anda Cornea,P. Michael Conn Pdf

Fluorescence Microscopy: Super-Resolution and other Novel Techniques delivers a comprehensive review of current advances in fluorescence microscopy methods as applied to biological and biomedical science. With contributions selected for clarity, utility, and reproducibility, the work provides practical tools for investigating these ground-breaking developments. Emphasizing super-resolution techniques, light sheet microscopy, sample preparation, new labels, and analysis techniques, this work keeps pace with the innovative technical advances that are increasingly vital to biological and biomedical researchers. With its extensive graphics, inter-method comparisons, and tricks and approaches not revealed in primary publications, Fluorescence Microscopy encourages readers to both understand these methods, and to adapt them to other systems. It also offers instruction on the best visualization to derive quantitative information about cell biological structure and function, delivering crucial guidance on best practices in related laboratory research. Presents a timely and comprehensive review of novel techniques in fluorescence imaging as applied to biological and biomedical research Offers insight into common challenges in implementing techniques, as well as effective solutions

Author : Ata Mahjoubfar,Claire Lifan Chen,Bahram Jalali
Publisher : Springer
Page : 134 pages
File Size : 52,6 Mb
Release : 2017-04-19
Category : Technology & Engineering
ISBN : 9783319514482

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Artificial Intelligence in Label-free Microscopy by Ata Mahjoubfar,Claire Lifan Chen,Bahram Jalali Pdf

This book introduces time-stretch quantitative phase imaging (TS-QPI), a high-throughput label-free imaging flow cytometer developed for big data acquisition and analysis in phenotypic screening. TS-QPI is able to capture quantitative optical phase and intensity images simultaneously, enabling high-content cell analysis, cancer diagnostics, personalized genomics, and drug development. The authors also demonstrate a complete machine learning pipeline that performs optical phase measurement, image processing, feature extraction, and classification, enabling high-throughput quantitative imaging that achieves record high accuracy in label -free cellular phenotypic screening and opens up a new path to data-driven diagnosis.

Author : Robert D. Goldman,David L. Spector
Publisher : CSHL Press
Page : 658 pages
File Size : 55,6 Mb
Release : 2005
Category : Science
ISBN : 0879696834

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Live Cell Imaging by Robert D. Goldman,David L. Spector Pdf

Recent advances in imaging technology reveal, in real time and great detail, critical changes in living cells and organisms. This manual is a compendium of emerging techniques, organized into two parts: specific methods such as fluorescent labeling, and delivery and detection of labeled molecules in cells; and experimental approaches ranging from the detection of single molecules to the study of dynamic processes in organelles, organs, and whole animals. Although presented primarily as a laboratory manual, the book includes introductory and background material and could be used as a textbook in advanced courses. It also includes a DVD containing movies of living cells in action, created by investigators using the imaging techniques discussed in the book. The editors, David Spector and Robert Goldman, whose previous book was Cells: A Laboratory Manual,are highly respected investigators who have taught microscopy courses at Cold Spring Harbor Laboratory, the Marine Biology Laboratory at Woods Hole, and Northwestern University.