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Author : Nicolas L. Taylor,A. Harvey Millar
Publisher : Methods in Molecular Biology
Page : 352 pages
File Size : 50,8 Mb
Release : 2018-10-26
Category : Science
ISBN : 1493982281
Author : Zoë A. Popper
Publisher : Humana
Page : 0 pages
File Size : 49,7 Mb
Release : 2020-07-03
Category : Science
ISBN : 1071606190
This second edition volume expands on the previous edition with many new and updated chapters discussing the latest techniques used to investigate cell wall biochemistry, biomechanical properties, chemistry, and biology. Chapters in this book also cover topics such as cell wall composition and structure, plant tissue culture protoplast isolation, genetic manipulation, investigation of enzyme activities, and in situ localization of wall components. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, The Plant Cell Wall: Methods and Protocols, Second Edition is a valuable resource for both novice and expert scientists interested in learning more about this field.
Author : Bruce Alberts
Publisher : Unknown
Page : 0 pages
File Size : 55,5 Mb
Release : 2002
Category : Cytology
ISBN : 0815332181
Author : Paul R. Pryor
Publisher : Unknown
Page : 0 pages
File Size : 51,6 Mb
Release : 2015
Category : Cell fractionation
ISBN : 1621820424
Eukaryotic cells are remarkably complex structures, containing a vast repertoire of macromolecules, organelles, and other compartments that orchestrate the tasks required for life. For in-depth studies of their function and composition, reliable methods for the isolation of specific subcellular structures are often required. This laboratory manual provides step-by-step protocols for the extraction of subcellular components from animal tissues, yeasts, plants, and cultured cells. Each chapter focuses on a particular eukaryotic organelle, vesicle, membrane, or macromolecular complex. Strategies for breaking cells while maintaining the structural and functional integrity of the component of interest, enriching for that component based on its physical and biochemical characteristics, and monitoring and ensuring the success of the purification procedure are provided. The contributors describe both traditional approaches (e.g., density gradient centrifugation) and innovative techniques (e.g., the use of SPIONs) for isolating subcellular constituents. This manual is therefore an essential laboratory resource for all cell biologists seeking a comprehensive collection of dependable subcellular fractionation methods.
Author : Zoë A. Popper
Publisher : Lulu.com
Page : 324 pages
File Size : 41,6 Mb
Release : 2024-06-29
Category : Electronic
ISBN : 9780359580996
Author : Ralph Rapley,David L. Manning
Publisher : Humana Press
Page : 264 pages
File Size : 41,8 Mb
Release : 2013-08-18
Category : Science
ISBN : 1489942505
Ribonucleic acids are central to cellular and molecular processes and perform vital functions in both structural and functional roles. RNA molecules form the bridge between the stable genetic information contained within DNA and enzymes and proteins that carry out much of the metabolism within the cell. Many of the sites of protein synthesis, the ribosomes within the cell, are composed of these ribonucleic acids as are the tRNA molecules that deliver the amino acid building blocks to the ribosomes. Of all the RNA species, the nucleic acid intermediate, messenger RNA, is a desirable source of material to biologists, since this reflects much of, what ultimately, is translated into enzymes and proteins. In order to determine the qualitative and quantitative changes in mRNA expression, a vast number of molecular biological techniques have been developed. Key molecular methods that provide the means to initially isolate and analyze RNA molecules are the focus of this volume. In putting together this collection of protocols, we have tried to provide techniques that are most applicable and widely used. In particular, there are a number of iso- tion techniques included that have been developed, modified, or adapted to enable extraction from a variety of cell types, organisms, or subcellular organelles. Successful isolation of intact RNA is an essential starting point for any sub- quent analysis. This is why we have aimed to make this section comprehensive. The analysis of RNA is the focus of the following chapters.
Author : William V. Dashek,Gurbachan S. Miglani
Publisher : John Wiley & Sons
Page : 426 pages
File Size : 51,6 Mb
Release : 2017-01-17
Category : Science
ISBN : 9780470976869
Plant Cells and Their Organelles provides a comprehensive overview of the structure and function of plant organelles. The text focuses on subcellular organelles while also providing relevant background on plant cells, tissues and organs. Coverage of the latest methods of light and electron microscopy and modern biochemical procedures for the isolation and identification of organelles help to provide a thorough and up-to-date companion text to the field of plant cell and subcellular biology. The book is designed as an advanced text for upper-level undergraduate and graduate students with student-friendly diagrams and clear explanations.
Author : B.K. Garg
Publisher : Scientific Publishers
Page : 571 pages
File Size : 46,8 Mb
Release : 2012-06-01
Category : Technology & Engineering
ISBN : 9789387307872
The book `Plant Analysis: Comprehensive Methods and Protocols' is a complete laboratory manual for analytical methods and techniques in the field of Agriculture, Plant Physiology, Biochemistry and related Plant Sciences. Right from nutrient analysis in plants, it covers estimations of macromolecules, such as amino acids, proteins, nucleic acids and metabolites of fatty acid metabolism. Protocols for the assay of various enzymes of nitrogen metabolism, ammonia assimilation, photosynthetic CO2-fixation, reactive oxygen species, carbohydrate, phosphorus and energy metabolism have been elucidated in the book. Special emphasis has also been given to techniques on specific topics such as Electrophoresis, Molecular Biology, Histo-enzymology, Symbiotic Nitrogen Fixation and assay of plant growth hormones. Thus the present book is one stop solution for all important techniques and analytical methods for students and research workers engaged in plant sciences and agricultural research.
Author : Flavia Vischi Winck
Publisher : Springer Nature
Page : 220 pages
File Size : 54,7 Mb
Release : 2022-02-03
Category : Science
ISBN : 9783030803520
In the post-genomic era, several plant species have been sequenced and massive genomic information is now available which contributed to expand the development of novel technical strategies for the study of additional levels of biological information of plant species. This book focuses on the “omics” approaches together with systems analysis of several different plant species, which have revealed very interesting variations on the cellular responses at the protein, transcript and metabolite levels in response to changes environmental conditions. The volume covers recent technological advances in the area of “omics” and synthesizes recent findings of the field of plant “omics” and systems biology together along with techniques that can be applied for such studies.
Author : Anonim
Publisher : Elsevier
Page : 464 pages
File Size : 51,7 Mb
Release : 1997-05-06
Category : Technology & Engineering
ISBN : 0080561799
Advances in Botanical Research is a multi-volume publication that brings together reviews by recognized experts on subjects of importance to those involved in botanical research. For more than thirty years, Advances in Botanical Research has earned a reputation for excellence in the field. For those working on plant pathology, Advances in Plant Pathology has also carved a niche in the plant sciences during its decade of publication.Academic Press has merged Advances in Plant Pathology into Advances in Botanical Research. The plant science community will find that the merger of these two serials will provide one comprehensive resource for the field. To ensure complete coverage, John Andrews and Inez Tommerup, the editors of Advances in Plant Pathology, have joined the editorial board of the new series, which will include equal coverage of plant pathology and botany in both thematic and mixed volumes. The first few volumes of the new series will be slanted toward botany or plant pathology; however, future eclectic volumes will be fully integrated.The resulting synergy of these two serials greatly benefits the plant science community by providing a more comprehensive resource under one roof. The joint aim is to continue to include the very best articles, thereby maintaining the status of a high impact factor review series.
Author : Dorothea Bartels,Peter Dörmann
Publisher : Humana
Page : 472 pages
File Size : 43,6 Mb
Release : 2022-06-12
Category : Science
ISBN : 1071613642
This volume explores analytical methods to study complex lipid mixtures from plants and algae. The chapters in this book are organized into five parts and cover topics such as basic methods of lipid isolation and analysis; mass spectrometry and NMR analysis; lipid isolation and analysis from plant tissues, cell compartments and organelles; lipid signaling, lipid-protein interactions, and imaging; and lipid databases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Plant Lipids: Methods and Protocols is a valuable guide for experienced researchers and undergraduate, graduate, and Ph.D. students. This book is also an excellent resource for novice scientists with little to no experience in lipid experiments who are interested in approaching this field experimentally.
Author : H. Smith,Harry Smith
Publisher : Univ of California Press
Page : 516 pages
File Size : 44,9 Mb
Release : 1977-01-01
Category : Science
ISBN : 0520034651
Plant cell structure and function; Gene expression and its regulation in plant cells; The manipulation of plant cells.
Author : Stanton Gelvin
Publisher : Springer Science & Business Media
Page : 138 pages
File Size : 48,6 Mb
Release : 2013-11-11
Category : Science
ISBN : 9789401152426
Author : Paul McCabe,Francois Bouteau,Arunika Gunawardena,Joanna Kacprzyk
Publisher : Frontiers Media SA
Page : 90 pages
File Size : 48,7 Mb
Release : 2021-05-28
Category : Science
ISBN : 9782889668120
Author : Verena Kriechbaumer
Publisher : Springer Nature
Page : 434 pages
File Size : 51,7 Mb
Release : 2024-06-29
Category : Electronic
ISBN : 9781071637104
