Rna Isolation And Characterization Protocols

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RNA Isolation and Characterization Protocols

Ralph Rapley,David L. Manning
RNA Isolation and Characterization Protocols Book PDF

Author : Ralph Rapley,David L. Manning
Publisher : Unknown
Page : 264 pages
File Size : 40,9 Mb
Release : 1998
Category : Science
ISBN : 1592595707

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RNA Isolation and Characterization Protocols by Ralph Rapley,David L. Manning Pdf

RNA Isolation and Characterization Protocols offers biological investigators a powerful and highly useful collection of up-to-date methods for the isolation of RNA from a variety of sources, including bacterial, plant, and mammalian cells. These fine-tuned protocols take both the experienced and inexperienced investigator through the nuances of RNA manipulation, from extraction to in vitro translation. The authoritative researchers writing here also include detailed protocols for the analysis and characterization of isolated RNA species and extensive troubleshooting hints and tips to ensure easy reproducibility. RNA Isolation and Characterization Protocols gives today's bench scientists the single best collection of easily reproducible, step-by-step RNA techniques now available. This book will quickly become indispensable for everyone seeking to manipulate RNA and help set the stage for the coming revolution in RNA-based diagnostic techniques.

RNA Methodologies

Robert E. Farrell, Jr.
RNA Methodologies Book PDF

Author : Robert E. Farrell, Jr.
Publisher : Elsevier
Page : 688 pages
File Size : 47,9 Mb
Release : 2010-07-22
Category : Science
ISBN : 9780080454764

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RNA Methodologies by Robert E. Farrell, Jr. Pdf

This laboratory guide represents a growing collection of tried, tested and optimized laboratory protocols for the isolation and characterization of eukaryotic RNA, with lesser emphasis on the characterization of prokaryotic transcripts. Collectively the chapters work together to embellish the RNA story, each presenting clear take-home lessons, liberally incorporating flow charts, tables and graphs to facilitate learning and assist in the planning and implementation phases of a project. RNA Methodologies, 3rd edition includes approximately 30% new material, including chapters on the more recent technologies of RNA interference including: RNAi; Microarrays; Bioinformatics. It also includes new sections on: new and improved RT-PCR techniques; innovative 5’ and 3’ RACE techniques; subtractive PCR methods; methods for improving cDNA synthesis. * Author is a well-recognized expert in the field of RNA experimentation and founded Exon-Intron, a well-known biotechnology educational workshop center * Includes classic and contemporary techniques * Incorporates flow charts, tables, and graphs to facilitate learning and assist in the planning phases of projects

RNA Methodologies

Bozzano G Luisa
RNA Methodologies Book PDF

Author : Bozzano G Luisa
Publisher : Academic Press
Page : 332 pages
File Size : 52,8 Mb
Release : 2012-12-02
Category : Science
ISBN : 9780323137799

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RNA Methodologies by Bozzano G Luisa Pdf

This book is a collection of tried and tested laboratory protocols for the isolation and characterization of mammalian RNA. It studies cellular regulation using RNA as a parameter of gene express, offers RNA isolation strategies, and explains proper handling, storage, and manipulation of RNA. * Studies cellular regulation using RNA as a parameter of Gene Expression * Offers RNA isolation strategies * Explains proper handling, storage, and manipulation of RNA

RNA Methodologies

Robert E. Farrell
RNA Methodologies Book PDF

Author : Robert E. Farrell
Publisher : Academic Press
Page : 717 pages
File Size : 48,7 Mb
Release : 2010
Category : Science
ISBN : 0123747279

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RNA Methodologies by Robert E. Farrell Pdf

This is the fourth edition of the successful laboratory guide which has translated the rich story of riboneucleic acid for over fifteen years. RNA Methodologies 4e presents the latest collection of tested laboratory protocols for the isolation and characterization of eukaryotic and prokaryotic RNA with greater emphasis on transcript profiling, including quantification issues and elucidation of alternative transcription start sites. Collectively the chapters work together providing analysis with clear take-home lessons to assist researchers to understand RNA and to optimize time at the bench. The abundant use of flow charts, tables and graphs are especially helpful in the planning and implementation phases of a project and facilitate learning. 30% new material in this edition includes the addition of RNA isolation protocols including RNA isolation from tissue, expansion of PCR optimization analysis and RNA interference sections, the introduction of a new chapter dealing with the molecular biology of plants, and an expanded glossary. * 30% new material with the addition of RNA isolation protocols including RNA isolation from tissue, expansion of PCR optimization analysis and RNA interference sections, the introduction of a new chapter dealing with the molecular biology of plants, and an expanded glossary * Author is a well-recognized expert in the field of RNA experimentation and founded Exon-Intron, a well-known biotechnology educational workshop center * Includes classic and contemporary techniques useful for all labs

RNA Isolation and Characterization Protocols

Ralph Rapley,David L. Manning
RNA Isolation and Characterization Protocols Book PDF

Author : Ralph Rapley,David L. Manning
Publisher : Humana Press
Page : 264 pages
File Size : 51,5 Mb
Release : 2013-08-18
Category : Science
ISBN : 1489942505

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RNA Isolation and Characterization Protocols by Ralph Rapley,David L. Manning Pdf

Ribonucleic acids are central to cellular and molecular processes and perform vital functions in both structural and functional roles. RNA molecules form the bridge between the stable genetic information contained within DNA and enzymes and proteins that carry out much of the metabolism within the cell. Many of the sites of protein synthesis, the ribosomes within the cell, are composed of these ribonucleic acids as are the tRNA molecules that deliver the amino acid building blocks to the ribosomes. Of all the RNA species, the nucleic acid intermediate, messenger RNA, is a desirable source of material to biologists, since this reflects much of, what ultimately, is translated into enzymes and proteins. In order to determine the qualitative and quantitative changes in mRNA expression, a vast number of molecular biological techniques have been developed. Key molecular methods that provide the means to initially isolate and analyze RNA molecules are the focus of this volume. In putting together this collection of protocols, we have tried to provide techniques that are most applicable and widely used. In particular, there are a number of iso- tion techniques included that have been developed, modified, or adapted to enable extraction from a variety of cell types, organisms, or subcellular organelles. Successful isolation of intact RNA is an essential starting point for any sub- quent analysis. This is why we have aimed to make this section comprehensive. The analysis of RNA is the focus of the following chapters.

RNA Isolation and Analysis

Paul Jones,Jane Qiu,David Rickwood
RNA Isolation and Analysis Book PDF

Author : Paul Jones,Jane Qiu,David Rickwood
Publisher : Taylor & Francis
Page : 196 pages
File Size : 51,9 Mb
Release : 1994
Category : Science
ISBN : 1872748376

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RNA Isolation and Analysis by Paul Jones,Jane Qiu,David Rickwood Pdf

The isolation and analysis of RNA plays a key role in molecular biology today. This comprehensive account of the key techniques provides the background information and the protocols used to isolate and analyse RNA. The book is essential reading for all re

RNA Methodologies

Robert E. Farrell Jr.
RNA Methodologies Book PDF

Author : Robert E. Farrell Jr.
Publisher : Elsevier
Page : 962 pages
File Size : 48,8 Mb
Release : 2022-11-22
Category : Science
ISBN : 9780323916561

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RNA Methodologies by Robert E. Farrell Jr. Pdf

RNA Methodologies: A Laboratory Guide for Isolation and Characterization, Sixth Edition provides the most up-to-date ribonucleic acid lab techniques for seasoned scientists and graduate students alike. This edition features new material on RNA sequencing, RNA in Situ Hybridization, non-coding RNAs, computational RNA biology, transcriptomes and bioinformatics, along with the latest advances in methods and protocols across the field of RNA investigation. As a leader in the field, Dr. Farrell provides a wealth of knowledge on the topic of RNA biology while also giving readers helpful hints and troubleshooting techniques from his own personal experience in this subject area. This book presents the essential knowledge and techniques to use when working with RNA for the experienced practitioner, while also aiding the beginner in fully understanding this important branch of molecular biology. Presents the latest information covering all aspects of working with RNA, delivering a holistic understanding of this leading field in molecular biology Builds from basic information on RNA techniques to in-depth protocols for specific applications Features new chapters on RNA sequencing and RNA in situ hybridization Includes new material on RNA clinical applications and innovations, including RNA therapeutics and RNA vaccines, with particular relevance to coronavirus Comprises the latest developments in transcriptomes and bioinformatics, with new material on computational RNA biology, RNA CHiP analysis, aptamer biology and RNA epigenetics

A Laboratory Guide to RNA

Paul A. Krieg
A Laboratory Guide to RNA Book PDF

Author : Paul A. Krieg
Publisher : John Wiley & Sons
Page : 470 pages
File Size : 46,8 Mb
Release : 1996-08-15
Category : Science
ISBN : 0471125369

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A Laboratory Guide to RNA by Paul A. Krieg Pdf

Here is the most complete guide available to the isolation, analysis, and synthesis of RNA. It covers everything researchers and laboratory workers need to know about the study of gene expression via RNA analysis-from the theory behind the methods, to actual problem-solving techniques. Step-by-step protocols are presented for each method. A careful presentation of the experimental formalities of these protocols enables specialists and nonspecialists alike to implement the methods easily in the laboratory. Each protocol is accompanied by the theoretical background underlying the experimental procedure and most chapters contain illustrations of typical results and troubleshooting tips. A Laboratory Guide to RNA offers a straightforward detailed account of experimental procedures, ranging from the isolation of RNA from a variety of cell and tissue types, detection analysis, and quantitation using a range of strategies, to large- and small-scale synthesis of RNA. This unique guide not only covers established procedures such as RNA blotting and nuclease protection, but also the latest protocols for quantitative PCR and differential display. Protocols addressing in situ hybridization are highlighted in an eight-page, full-color section that illustrates the power of the technique for detection of gene expression in tissues and whole organisms. Featuring contributions from leading research laboratories and the biotechnology field, A Laboratory Guide to RNA: Isolation, Analysis, and Synthesis provides all the methods required for RNA analysis. It is the ideal laboratory guide for research scientists, graduate students, and lab personnel who need a solid reference on the analysis of gene expression at the RNA level.

RNA Methodologies

Robert E. Farrell, Jr.
RNA Methodologies Book PDF

Author : Robert E. Farrell, Jr.
Publisher : Academic Press
Page : 876 pages
File Size : 51,5 Mb
Release : 2017-08-11
Category : Science
ISBN : 9780128046791

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RNA Methodologies by Robert E. Farrell, Jr. Pdf

RNA Methodologies, Fifth Edition continues its tradition of excellence in providing the most up-to-date ribonucleic acid lab techniques for seasoned scientists and graduate students alike. This edition features new material on the exploding field of microRNA as well as the methods for the profiling of gene expression, both which have changed considerably in recent years. As a leader in the field, Dr. Farrell provides a wealth of knowledge on the topic of RNA while also giving readers helpful hints from his own personal experience in this subject area. Beginning with the most contemporary, RNA Methodologies, Fifth Edition, presents the essential techniques to use when working with RNA for the experienced practitioner while at the same time providing images and examples to aid the beginner in fully understanding this important branch of molecular biology. The next generation of scientists can look to this work as a guide for ensuring high productivity and highly representative data, as well as best practices in troubleshooting laboratory problems when they arise. Features new material in miRNA, MIQE guidelines, biomarkers, RNA sequencing, digital PCR and more Includes expanded coverage on quantitative PCR techniques, RNAi, bioinformatics, the role of locked nucleic acids, aptamer biology, PCR arrays, and other modern technologies Presents comprehensive, cutting-edge information covering all aspects of working with RNA Builds from basic information on RNA techniques to in-depth protocols to guidance on how to modify and adjust each step of a particular application Presents multiple avenues for addressing the same experimental goals

Protocols for Nucleic Acid Analysis by Nonradioactive Probes

Elena Hilario,John F. MacKay
Protocols for Nucleic Acid Analysis by Nonradioactive Probes Book PDF

Author : Elena Hilario,John F. MacKay
Publisher : Springer Science & Business Media
Page : 318 pages
File Size : 43,9 Mb
Release : 2008-02-02
Category : Science
ISBN : 9781597452298

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Protocols for Nucleic Acid Analysis by Nonradioactive Probes by Elena Hilario,John F. MacKay Pdf

Protocols for Nucleic Acid Analysis by Non-radioactive Probes, Second Edition provides a firm background on the basic preparative protocols required for the analysis of nucleic acids by nonradioactive methods. Presenting the methodologies using amazing new applications, this volume offers guide chapters on nucleic acid extractions, preparation of nucleic acid blots, and labeling of nucleic acids with nonradioactive haptens. New fluorescent techniques such as Real Time PCR and microarrays are also included, allowing users to get a nonradioactive protocol implemented in the laboratory with minimum adaptation required and fastest time to results. The protocols follow the successful Methods in Molecular BiologyTM series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

RNA Purification and Analysis

Douglas T. Gjerde,Lee Hoang,David Hornby
RNA Purification and Analysis Book PDF

Author : Douglas T. Gjerde,Lee Hoang,David Hornby
Publisher : John Wiley & Sons
Page : 206 pages
File Size : 45,8 Mb
Release : 2009-07-10
Category : Science
ISBN : 9783527627202

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RNA Purification and Analysis by Douglas T. Gjerde,Lee Hoang,David Hornby Pdf

This first book on the market covers the many new and important RNA species discovered over the past five years, explaining current methods for the enrichment, separation and purification of these novel RNAs. Building up from general principles of RNA biochemistry and biophysics, this book addresses the practical aspects relevant to the laboratory researcher throughout, while discussing the performance and potential problems of the methods discussed. An appendix contains a glossary with the important terms and techniques used in RNA analysis. By explaining the basic and working principles of the methods, the book allows biochemists and molecular biologists to gain much more expertise than by simply repeating a pre-formulated protocol, enabling them to select the procedure and materials best suited to the RNA analysis task at hand. As a result, they will be able to develop new protocols where needed and optimize and fine-tune the general purpose standard protocols that come with the purification equipment and instrumentation.

The Nucleic Acid Protocols Handbook

Ralph Rapley
The Nucleic Acid Protocols Handbook Book PDF

Author : Ralph Rapley
Publisher : Springer Science & Business Media
Page : 478 pages
File Size : 48,8 Mb
Release : 2008-06-29
Category : Science
ISBN : 9781592590384

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The Nucleic Acid Protocols Handbook by Ralph Rapley Pdf

A comprehensive treasury of all the key molecular biology methods-ranging from DNA extraction to gene localization in situ-needed to function effectively in the modern laboratory. Each of the 120 highly successful techniques follows the format of the much acclaimed Methods in Molecular BiologyOao series, providing an introduction to the scientific basis of each technique, a complete listing of all the necessary materials and reagents, and clear step-by-step instruction to permit error-free execution. Included for each technique are notes about pitfalls to avoid, troubleshooting tips, alternate methods, and explanations of the reasons for certain steps-all key elements contributing significantly to success or failure in the lab. The Nucleic Acid Protocols Handbook constitutes today's most comprehensive collection of all the key classic and cutting-edge techniques for the successful isolation, analysis, and manipulation of nucleic acids by both experienced researchers and those new to the field."

MicroRNA Profiling

Sweta Rani
MicroRNA Profiling Book PDF

Author : Sweta Rani
Publisher : Humana
Page : 0 pages
File Size : 47,8 Mb
Release : 2023-12-13
Category : Science
ISBN : 1071628259

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MicroRNA Profiling by Sweta Rani Pdf

This second edition provides updated and comprehensive methods on miRNA biogenesis and their role in the development and progression of various human diseases. Chapters detail miRNA biogenesis, isolating RNA, extracellular vesicles (EVs), circulating miRNAs, analyzing miRNA and miRDeep-P2, protocols for total RNA isolation from cells, cell-derived products, isolation and characterization of exosomes, serum, plasma specimens, and software tools. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, MicroRNA Profiling: Methods and Protocols, Second Edition aims to provide comprehensive and accessible methods to undergraduate, graduate, and established scientist.

RNA Amplification and Analysis

Mekbib Astatke
RNA Amplification and Analysis Book PDF

Author : Mekbib Astatke
Publisher : Humana
Page : 0 pages
File Size : 49,9 Mb
Release : 2024-08-11
Category : Science
ISBN : 107163917X

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RNA Amplification and Analysis by Mekbib Astatke Pdf

This volume provides methods to analyze RNA and gain insight to a number of critical cellular functions. Chapters detail RNA isolation and enrichment, RNA amplification and detection, RNA sequencing, analysis of RNA modification, RNA delivery approaches, and analysis of RNA binding and catalytic activity. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, RNA Amplifications and Analysis: Methods and Protocols aims to present researchers with cutting-edge technologies with detailed explanations of critical steps, while providing a clear understanding of the overall protocol.

RNA-Protein Interaction Protocols

Ren-Jang Lin
RNA Protein Interaction Protocols Book PDF

Author : Ren-Jang Lin
Publisher : Humana Press
Page : 0 pages
File Size : 51,5 Mb
Release : 2008-08-25
Category : Science
ISBN : 1588294196

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RNA-Protein Interaction Protocols by Ren-Jang Lin Pdf

Due to the vital biological importance of RNA and proteins functioning together within a cell, a protocol volume describing experimental procedures to study their interactions should find a home in many laboratories. RNA-Protein Interaction Protocols, Second Edition updates, complements, and expands upon the popular first edition by providing a collection of cutting-edge techniques developed or refined in the past few years along with tried-and-true methods. The expert contributors explore the isolation and characterization of RNA-protein complexes, the analysis and measurement of RNA-protein interaction, and related novel techniques and strategies. Written in the highly successful Methods in Molecular BiologyTM series format, the chapters include brief introductions to the material, lists of necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and a Notes section which highlights tips on troubleshooting and avoiding known pitfalls. Comprehensive and up-to-date, RNA-Protein Interaction Protocols, Second Edition is an ideal guide for researchers continuing the study of this all-important biological partnership.