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Author : Thomas Spence Work,Elizabeth Work
Publisher : Unknown
Page : 408 pages
File Size : 51,9 Mb
Release : 1999
Category : Biochemistry
ISBN : UOM:39015045481499
Author : Theodorus W. J. Gadella
Publisher : Elsevier
Page : 560 pages
File Size : 43,7 Mb
Release : 2011-07-29
Category : Science
ISBN : 0080915124
This volume reviews the techniques Förster Resonance Energy Transfer (FRET) and Fluorescence Lifetime Imaging Microscopy (FLIM) providing researchers with step by step protocols and handy hints and tips. Both have become staple techniques in many biological and biophysical fields.
Author : Keith Wilson,John Walker
Publisher : Cambridge University Press
Page : 769 pages
File Size : 48,7 Mb
Release : 2010-03-04
Category : Medical
ISBN : 9780521516358
Uniquely integrates the theory and practice of key experimental techniques for bioscience undergraduates. Now includes drug discovery and clinical biochemistry.
Author : Anonim
Publisher : Unknown
Page : 460 pages
File Size : 45,8 Mb
Release : 1969
Category : Biochemistry
ISBN : 0444813020
Author : Anonim
Publisher : Elsevier
Page : 486 pages
File Size : 55,7 Mb
Release : 2011-08-31
Category : Science
ISBN : 0080553508
Cell separation is at the core of current methods in experimental biology and medicine. Its importance is illustrated by the large number of physical and biochemical principles that have been evaluated for application to cell separation. The development of cell separation methods is driven by the needs of biological and medical research, and the ever-increasing demands for sensitivity, selectivity, yield, timeliness and economy of the process. The interdisciplinary nature of research in this area and the volume of information available in research publications and conferences necessitates a basic description of the fundamental processes involved in magnetic cell separation that may help the user in navigating this wealth of information available online and in scientific publications. This book will appeal to researchers in many areas utilizing this technique, including those working in cell biology, clinical research, inorganic chemistry, biochemistry, chemical engineering, materials science, physics and electrical engineering. Provides examples of how to calculate the volume magnetic susceptibility, a fundamental quantity for calculating the magnetic force acting on a cell, from various types of magnetic susceptibilities available in literature Introduces the elements of magnetostatics as they apply to cell magnetization and the magnetization of magnetic micro- and nano- particles used for cell separation Describes the parameters used to determine cell magnetophoresis
Author : P. Tijssen
Publisher : Elsevier
Page : 548 pages
File Size : 54,6 Mb
Release : 1985-08-01
Category : Medical
ISBN : 9780080858845
Enzyme immunoassays have developed into a powerful assay technology, transcending several discipline boundaries, extensively applied as a tool in fields other than enzymology and immunology. This volume reflects the rapid progress in the applications of this technique, providing a basic understanding of these techniques and a practical guideline for the choice and experimental detail.
Author : Anonim
Publisher : Unknown
Page : 425 pages
File Size : 41,8 Mb
Release : 1989
Category : Biochemistry
ISBN : 0444810226
Author : Catherine A. Rice- Evans
Publisher : Unknown
Page : 128 pages
File Size : 40,9 Mb
Release : 1991
Category : Electronic
ISBN : 0720442001
Author : Heather Miller,D. Scott Witherow,Sue Carson
Publisher : Academic Press
Page : 232 pages
File Size : 42,9 Mb
Release : 2011-10-18
Category : Science
ISBN : 9780123855459
This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The third edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The “project approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein - students can actually visualize positive clones following IPTG induction. Cover basic concepts and techniques used in molecular biology research labs Student-tested labs proven successful in a real classroom laboratories Exercises simulate a cloning project that would be performed in a real research lab "Project" approach to experiments gives students an overview of the entire process Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions
Author : Keith Wilson,Andreas Hofmann,John M. Walker,Samuel Clokie
Publisher : Cambridge University Press
Page : 961 pages
File Size : 54,5 Mb
Release : 2018-04-19
Category : Medical
ISBN : 9781107162273
A major update of a best-selling textbook that introduces students to the key experimental and analytical techniques underpinning life science research.
Author : A. H. Gordon
Publisher : Elsevier Science & Technology
Page : 220 pages
File Size : 53,9 Mb
Release : 1975
Category : Science
ISBN : 0444108645
Author : A. Kuksis
Publisher : Gulf Professional Publishing
Page : 1006 pages
File Size : 55,6 Mb
Release : 2003-09-12
Category : Medical
ISBN : 0444513043
The development of advanced methods for isolation, identification and quantification of old and new inositol lipids and inositol phosphtes from natural and synthetic systems has been a major advancing force in phosphoinositol research. The writing of this book was undertaken as an opportunity fo examine the analytical validity of the biochemical transformations that constitute the basis of the lipid signaling pathways.
Author : A.T. Diplock,M.C.R. Symons,C.A. Rice-Evans
Publisher : Elsevier
Page : 290 pages
File Size : 54,7 Mb
Release : 1991-12-11
Category : Science
ISBN : 0080858910
Free radical species are generally short-lived due to their high reactivity and thus direct measurement and identification are often impossible. ESR is the only technique which has the potential for direct detection of radicals but in biological systems even these must be trapped by a spin-trapping agent. Thus most investigations involve recognition of indicators of the presence of radicals in vivo or "FOOTPRINTS" of radical-mediated damage. Techniques in Free Radical Research assembles and critically assesses the most relevant and reliable experimental approaches used towards the measurement of radicals and radical-mediated damage in chemical systems, in cells and in tissues under the following six headings: a) Footprints of DNA damage, b) Footprints of protein damage, c) Footprints of lipid peroxidation, d) Footprints of antioxidant consumption, e) Footprints via indirect radical assays, and f) Footprints via the availability of transition metal complexes.
Author : M. H. V. Van Regenmortel
Publisher : Unknown
Page : 381 pages
File Size : 50,5 Mb
Release : 1969
Category : Antigenic determinants
ISBN : LCCN:68054514
Author : Alexander J. Ninfa,David P. Ballou,Marilee Benore
Publisher : John Wiley & Sons
Page : 480 pages
File Size : 54,7 Mb
Release : 2009-05-26
Category : Science
ISBN : 9780470087664
Ninfa/Ballou/Benore is a solid biochemistry lab manual, dedicated to developing research skills in students, allowing them to learn techniques and develop the organizational approaches necessary to conduct laboratory research. Ninfa/Ballou/Benore focuses on basic biochemistry laboratory techniques with a few molecular biology exercises, a reflection of most courses which concentrate on traditional biochemistry experiments and techniques. The manual also includes an introduction to ethics in the laboratory, uncommon in similar manuals. Most importantly, perhaps, is the authors' three-pronged approach to encouraging students to think like a research scientist: first, the authors introduce the scientific method and the hypothesis as a framework for developing conclusive experiments; second, the manual's experiments are designed to become increasingly complex in order to teach more advanced techniques and analysis; finally, gradually, the students are required to devise their own protocols. In this way, students and instructors are able to break away from a "cookbook" approach and to think and investigate for themselves. Suitable for lower-level and upper-level courses; Ninfa spans these courses and can also be used for some first-year graduate work.