Mass Spectrometry Of Proteins And Peptides

Author : Pradip K. Ghosh
Publisher : Elsevier
Page : 388 pages
File Size : 50,5 Mb
Release : 2024-04-26
Category : Science
ISBN : 9780443139208

Get Book

Introduction to Protein Mass Spectrometry by Pradip K. Ghosh Pdf

Introduction to Protein Mass Spectrometry, Second Edition provides a comprehensive overview of this increasingly important, yet complex, analytical technique. This book enables readers to understand how determinations about protein identity from mass spectrometric data are made. Coverage begins with the technical basics, including preparations, instruments, and spectrometric analysis of peptides and proteins, before exploring applied use in biological applications, bioinformatics, database, and software resources. This new edition is fully updated to include the latest developments in the field and will feature new content covering recent progress in the areas where there have been the most exciting advances. These include PNNL’s multilevel-PCB-based SLIM realization, SLIM-Agilent QQQ field trials; employment of SLIM-IMS-cryo-IR combination in molecular structure determination; proximity-labelling mass spectrometry, and applications in neuroscience. Offers up-to-date, introductory information for scientists and researchers new to the field, as well as advanced insights into the critical assessment of computer-analyzed mass spectrometric results and their current limitations Provides examples of commonly used MS instruments from a range of key manufacturers/developers, including Bruker, Applied Biosystems, JEOL, Thermo Scientific/Thermo Fisher Scientific, IU, Waters and PNNL Includes biological applications and exploration of analytical tools and databases for bioinformatics Features definitions, case studies, and recent developments in protein mass spectrometry Includes sections new to this edition on SLIM (Structures for Lossless Ion Manipulation) and mass spectrometry applications in neuroscience, including synaptic biology and Alzheimer’s disease

Author : John R. Chapman
Publisher : Springer Science & Business Media
Page : 539 pages
File Size : 52,9 Mb
Release : 2008-02-05
Category : Science
ISBN : 9781592590452

Get Book

Mass Spectrometry of Proteins and Peptides by John R. Chapman Pdf

Little more than three years down the line and I am already writing the Preface to a second volume to follow Protein and Peptide Analysis by Mass . What has happened in between these times to make this second venture worthwhile? New types of mass spectrometric instrumentation have appeared so that new techniques have become possible and existing techniques have become much more feasible. More particularly, however, the newer ionization te- niques, introduced for the analysis of high molecular weight materials, have now been thoroughly used and studied. As a result, there has been an en- mous improvement in the associated sample handling technology so that these methods are now routinely applied to much smaller sample amounts as well as to more intractable samples. Again, this particular community of mass spectrometry users has both increased in number and diversified. And, riding this wave of acceptance, leaders in the field have set their sights on more complex problems: molecular interaction, ion structures, quantitation, and kinetics are just a few of the newer areas reported in Mass Spectrometry of Proteins and Peptides. As with the first volume, one purpose of this collection, Mass Spectr- etry of Proteins and Peptides, is to show the reader what can be done by the application of mass spectrometry, and perhaps even to encourage the reader to venture down new paths.

Author : Michael L. Gross,Guodong Chen,Birendra Pramanik
Publisher : John Wiley & Sons
Page : 484 pages
File Size : 43,8 Mb
Release : 2011-09-26
Category : Medical
ISBN : 9781118116548

Get Book

Protein and Peptide Mass Spectrometry in Drug Discovery by Michael L. Gross,Guodong Chen,Birendra Pramanik Pdf

The book that highlights mass spectrometry and its application in characterizing proteins and peptides in drug discovery An instrumental analytical method for quantifying the mass and characterization of various samples from small molecules to large proteins, mass spectrometry (MS) has become one of the most widely used techniques for studying proteins and peptides over the last decade. Bringing together the work of experts in academia and industry, Protein and Peptide Mass Spectrometry in Drug Discovery highlights current analytical approaches, industry practices, and modern strategies for the characterization of both peptides and proteins in drug discovery. Illustrating the critical role MS technology plays in characterizing target proteins and protein products, the methods used, ion mobility, and the use of microwave radiation to speed proteolysis, the book also covers important emerging applications for neuroproteomics and antigenic peptides. Placing an emphasis on the pharmaceutical industry, the book stresses practice and applications, presenting real-world examples covering the most recent advances in mass spectrometry, and providing an invaluable resource for pharmaceutical scientists in industry and academia, analytical and bioanalytical chemists, and researchers in protein science and proteomics.

Author : J. R. Chapman
Publisher : Springer Science & Business Media
Page : 368 pages
File Size : 53,9 Mb
Release : 1996-08-19
Category : Medical
ISBN : UOM:39015038546373

Get Book

Protein and Peptide Analysis by Mass Spectrometry by J. R. Chapman Pdf

Leading practitioners authoritatively describe the newest and most effective spectrometric techniques for the analysis of proteins and peptides. The areas covered range from the elucidation of primary and secondary protein structure and the rapid identification of proteins using database techniques to methods for sequencing, as well as methods for the quantitative determination of peptides. Other chapters provide detailed information on the analysis of glycoproteins and glycopeptides and on the use of mass spectrometry to probe the interactions of proteins, both covalent and noncovalent.

Author : Mary S. Lipton,Ljiljana Pa#a-Tolic
Publisher : Humana Press
Page : 0 pages
File Size : 41,6 Mb
Release : 2014-11-27
Category : Science
ISBN : 1627037969

Get Book

Mass Spectrometry of Proteins and Peptides by Mary S. Lipton,Ljiljana Pa#a-Tolic Pdf

When the last edition of this book was published in 2000, the field of proteomics was in its infancy. At that time, multidimensional liquid chromatographic separations were being introduced as an alternative to traditional gel-based techniques for separating complex protein and peptide mixtures prior to mass spectrometric detection. Today, this approach – referred to as shotgun proteomics – is considered routine for lar- scale global analyses of protein mixtures. Now in its adolescence, proteomics is fundamentally transforming biological and medical research. Much of this transformation can be attributed to technological advancements, particularly in mass spectrometry. Much wider accessibility of hi- resolution and mass measurement accuracy instrumentation in recent years has ini- ated a new revolution in the field by providing more reliable data and shifting the focus from cataloging proteins to precisely quantifying changes in protein abundance over time and in response to stimuli. Advanced mass spectrometers and novel ion d- sociation schemes such as electron transfer/capture dissociation make it possible to venture boldly into the maze of protein posttranslational modifications, which are an integral component of understanding functional proteomics in the spatial and t- poral domains. Another area that has benefited from these advancements is top-down proteomics, an emerging method essential for characterizing various protein variants that has potentially high impact in biomedical research.

Author : Oscar Alzate
Publisher : CRC Press
Page : 354 pages
File Size : 51,8 Mb
Release : 2009-10-26
Category : Science
ISBN : 1420076264

Get Book

Neuroproteomics by Oscar Alzate Pdf

In this, the post-genomic age, our knowledge of biological systems continues to expand and progress. As the research becomes more focused, so too does the data. Genomic research progresses to proteomics and brings us to a deeper understanding of the behavior and function of protein clusters. And now proteomics gives way to neuroproteomics as we begin to unravel the complex mysteries of neurological diseases that less than a generation ago seemed opaque to our inquiries, if not altogether intractable. Edited by Dr. Oscar Alzate, Neuroproteomics is the newest volume in the CRC Press Frontiers of Neuroscience Series. With an extensive background in mathematics and physics, Dr. Alzate exemplifies the newest generation of biological systems researchers. He organizes research and data contributed from all across the world to present an overview of neuroproteomics that is practical and progressive. Bolstered by each new discovery, researchers employing multiple methods of inquiry gain a deeper understanding of the key biological problems related to brain function, brain structure, and the complexity of the nervous system. This in turn is leading to new understanding about diseases of neurological deficit such as Parkinson’s and Alzheimer’s. Approaches discussed in the book include mass spectrometry, electrophoresis, chromatography, surface plasmon resonance, protein arrays, immunoblotting, computational proteomics, and molecular imaging. Writing about their own work, leading researchers detail the principles, approaches, and difficulties of the various techniques, demonstrating the questions that neuroproteomics can answer and those it raises. New challenges wait, not the least of which is the identification of potential methods to regulate the structures and functions of key protein interaction networks. Ultimately, those building on the foundation presented here will advance our understanding of the brain and show us ways to abate the suffering caused by neurological and mental diseases.

Author : A.L. Burlingame
Publisher : Gulf Professional Publishing
Page : 520 pages
File Size : 48,7 Mb
Release : 2005-11-28
Category : Science
ISBN : 0121828077

Get Book

Biological Mass Spectrometry by A.L. Burlingame Pdf

Describes and integrates the techniques of many advances in both chromatographic and mass spectrometric technologies. This book also covers various biophysical applications, such as H/D exchange for study of conformations, protein-protein and protein-metal and ligand interactions. It also describes atto-to-zepto-mole quantitation of 14C and 3H.

Author : Rune Matthiesen
Publisher : Springer Science & Business Media
Page : 322 pages
File Size : 51,7 Mb
Release : 2008-02-02
Category : Science
ISBN : 9781597452755

Get Book

Mass Spectrometry Data Analysis in Proteomics by Rune Matthiesen Pdf

This is an in-depth guide to the theory and practice of analyzing raw mass spectrometry (MS) data in proteomics. The volume outlines available bioinformatics programs, algorithms, and databases available for MS data analysis. General guidelines for data analysis using search engines such as Mascot, Xtandem, and VEMS are provided, with specific attention to identifying poor quality data and optimizing search parameters.

Author : Mike S. Lee,Qin C. Ji
Publisher : John Wiley & Sons
Page : 288 pages
File Size : 53,7 Mb
Release : 2017-05-26
Category : Science
ISBN : 9781119359364

Get Book

Protein Analysis using Mass Spectrometry by Mike S. Lee,Qin C. Ji Pdf

Presents Practical Applications of Mass Spectrometry for Protein Analysis and Covers Their Impact on Accelerating Drug Discovery and Development Covers both qualitative and quantitative aspects of Mass Spectrometry protein analysis in drug discovery Principles, Instrumentation, Technologies topics include MS of peptides, proteins, and ADCs , instrumentation in protein analysis, nanospray technology in MS protein analysis, and automation in MS protein analysis Details emerging areas from drug monitoring to patient care such as Identification and validation of biomarkers for cancer, targeted MS approaches for biomarker validation, biomarker discovery, and regulatory perspectives Brings together the most current advances in the mass spectrometry technology and related method in protein analysis

Author : Thomas Letzel
Publisher : Royal Society of Chemistry
Page : 352 pages
File Size : 46,9 Mb
Release : 2011-07-22
Category : Science
ISBN : 9781849733144

Get Book

Protein and Peptide Analysis by LC-MS by Thomas Letzel Pdf

This book is the first example in presenting LC-MS strategies for the analysis of peptides and proteins with detailed information and hints about the needs and problems described from experts on-the-job. The best advantage is -for sure- the practical insight of experienced analysts into their novel protein analysis techniques. Readers starting in 'Proteomics' should be able to repeat each experiment with own equipment and own protein samples, like clean-up, direct protein analysis, after (online) digest, with modifications and others. Furthermore, the reader will learn more about strategies in protein analysis, like quantitative analysis, industrial standards, functional analysis and more.

Author : Paul T. Matsudaira
Publisher : Elsevier
Page : 205 pages
File Size : 54,7 Mb
Release : 2012-12-02
Category : Science
ISBN : 9780080924618

Get Book

A Practical Guide to Protein and Peptide Purification for Microsequencing by Paul T. Matsudaira Pdf

Why a Second Edition? The Second Edition provides practical answers to the general question, "How can I obtain useful sequence information from my protein or peptide?" rather than the more specific question asked in the first edition, "How can I obtain the N-terminal sequence?" Important new methods include ways of dealing with blocked N termini, computer analysis of protein sequences, and the recent revolution in mass spectrometry. Mass spectrophotometric characterization of proteins and peptides N-terminal sequencing of proteins with blocked N termini Internal amino acid sequence analysis after protease digestion in-gel and on-blot Improved microscale peptide purification methods Computer analysis of protein sequences New protocols tested and refined through everyday use in authors' laboratories Updated reference chapter covering all aspects of protein microsequencing

Author : John R. Chapman
Publisher : Humana Press
Page : 350 pages
File Size : 50,8 Mb
Release : 2013-08-11
Category : Science
ISBN : 1489940731

Get Book

Protein and Peptide Analysis by Mass Spectrometry by John R. Chapman Pdf

The purpose of the preface is to explain the book's objectives and how to use it; give warnings, disclaimers, and the like.* The main objective of Protein and Peptide Analysis by Mass Spec trometry is quite straightforward—to present authoritative, up-to-date, and practical accounts of the use of mass spectrometry in the analysis of pep tides and proteins. How to use it? Every reader will have their own particular interests and will surely be drawn toward the chapters that cover these interests. Within the remaining chapters, however, techniques are described with analytical possibilities that such a reader can then only guess at. So, read the book fully. Again, as is customary in the Methods in Molecular Biology series, the chapter format (Introduction, Materials, Methods, and Notes) allows the authors to introduce the techniques, to explain their relevance and applicability, and, above all, to provide detail—detail that represents each author's accumulated experience and enables the reader to use and benefit from these methods. So, read the book fully, and read it diligently. Warnings and disclaimers: Mass spectrometry today offers the pro tein chemist ready access to a wealth of information that is otherwise avail able only with great difficulty, or perhaps not at all. With this goal in sight, any warnings and disclaimers will almost surely be ignored. So, a warning anyway; the use of mass spectrometry might be habit forming.

Author : William S. Hancock
Publisher : CRC Press
Page : 280 pages
File Size : 40,5 Mb
Release : 1995-10-23
Category : Medical
ISBN : 0849378222

Get Book

New Methods in Peptide Mapping for the Characterization of Proteins by William S. Hancock Pdf

This text is devoted to the characterization of recombinant DNA-derived proteins by peptide mapping. It describes new technological procedures including capillary electrophoresis, analysis of glycopeptides and the use of electrospray and matrix-assisted laser desorption mass spectrometry. The book presents practical procedures for preparing a protein sample, the enzyme digestion, choice of separation method and procedures for the structural analysis of the separated species. Many figures of peptide maps illustrate typical results. Tables of summary information about digestion, separation conditions, and analyses of important protein samples are also presented.

Author : C. J McNeal
Publisher : Unknown
Page : 344 pages
File Size : 47,7 Mb
Release : 1988-12-26
Category : Science
ISBN : UOM:39015016067483

Get Book

Analysis of Peptides and Proteins by Mass Spectrometry by C. J McNeal Pdf

Comprises the proceedings of the Fourth Texas Symposium on Mass Spectrometry, held at Texas A & M University, April 1988. Twenty-five papers, presented by an international gathering of mass spectroscopists and bioscientists, reveal the state of the art in the application of mass spectrometry to the life sciences. Discussed are instruments that are sensitive to femtomoles, how mass spectrometry can further peptide mapping, monitoring peptide synthesis, peptide sequencing, and much more.

Author : C. J. McNeal
Publisher : Unknown
Page : 0 pages
File Size : 55,9 Mb
Release : 1988
Category : Electronic
ISBN : 0608068306

Get Book

The Analysis of Peptides and Proteins by Mass Spectrometry by C. J. McNeal Pdf