Matrix Metalloproteinase Protocols

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Matrix Metalloproteinase Protocols

Ian M. Clark
Matrix Metalloproteinase Protocols Book PDF

Author : Ian M. Clark
Publisher : Springer Science & Business Media
Page : 547 pages
File Size : 53,7 Mb
Release : 2008-02-05
Category : Science
ISBN : 9781592590469

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Matrix Metalloproteinase Protocols by Ian M. Clark Pdf

Research in the matrix metalloproteinase field began with the demonstration by Gross and Lapière, in 1962, that resorbing tadpole tail expressed an enzyme that could degrade collagen gels. These humble beginnings have led us to the elucidation of around twenty distinct vertebrate MMPs, along with a variety of homologs from such diverse organisms as sea urchin, plants, nematode worm, and bacteria. This, coupled with four known specific inhibitors of MMPs, the TIMPs, gives a complex picture. Part I of Matrix Metalloproteinase Protocols provides the reader with a selective overview of the MMP arena, and a chance to come to grips with where the field has been, where it is, and where it is going. I hope that this complements all of the methodology that comes later. Part II presents the reader with a diverse set of methods for the expression and purification of MMPs and TIMPs, bringing together the long and often hard-earned experience of a number of researchers. Part III allows the reader to detect MMPs and TIMPs at both the protein and mRNA level, whereas Part IV gives the ability to assay MMP and TIMP activities in a wide variety of circumstances.

Matrix Metalloproteases

Charles A. Galea
Matrix Metalloproteases Book PDF

Author : Charles A. Galea
Publisher : Humana
Page : 314 pages
File Size : 41,6 Mb
Release : 2018-08-07
Category : Science
ISBN : 1493983210

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Matrix Metalloproteases by Charles A. Galea Pdf

Since the identification of the first matrix metalloprotease (insterstitial collagenase or MMP-1) more than 20 closely related and evolutionarily conserved vertebrate MMPs have been discovered. Chapters in this volume detail methods and protocols on recent advances in the expression and purification of MMPs, biophysical methods such as X-ray crystallography, NMR spectroscopy and small angle X-ray scattering, computational and experimental methods for determining MMP specificity, methods for detecting MMP activity in vitro and in vivo, and methods for the development and characterization of MMP-based inhibitors as potential therapeutics for the treatment of various diseases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Matrix Metalloproteases: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Matrix Metalloproteinase Protocols

Ian M. Clark
Matrix Metalloproteinase Protocols Book PDF

Author : Ian M. Clark
Publisher : Humana Press
Page : 0 pages
File Size : 54,6 Mb
Release : 2010-03-04
Category : Science
ISBN : 1603272984

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Matrix Metalloproteinase Protocols by Ian M. Clark Pdf

Since the discovery of a collagen-degrading protease in the tadpole tail in 1962, matrix metalloproteinase research has led to the discovery of more than twenty distinct vertebrate MMPs, along with a variety of homologues from diverse organisms such as the sea urchin, plants, insects, and nematode worms. Fully updating and adding to the popular first edition, Matrix Metalloproteinase Protocols, Second Edition includes a series of state-of-the-art techniques provided by eminent experts in the field. Beginning with a brief overview of the MMP arena, from how these enzymes fit into the larger degradome to what occurs when their expression and function in the mouse is modulated, the volume continues with sections on the expression and purification of MMPs and TIMPs, the detection of MMPs and TIMPs at both the protein and mRNA level, and our ability to assay MMP and TIMP activities in a wide variety of circumstances. Written in the highly successful Methods in Molecular BiologyTM series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Matrix Metalloproteinase Protocols, Second Edition is an ideal source for many of the essential laboratory techniques for both novice and seasoned researchers alike collected in one convenient volume.

Inflammation Protocols

Paul G. Winyard,Derek A. Willoughby
Inflammation Protocols Book PDF

Author : Paul G. Winyard,Derek A. Willoughby
Publisher : Springer Science & Business Media
Page : 373 pages
File Size : 43,7 Mb
Release : 2008-02-03
Category : Medical
ISBN : 9781592593743

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Inflammation Protocols by Paul G. Winyard,Derek A. Willoughby Pdf

Inflammation has been described as the basis of many pathologies of human disease. When one considers the updated signs of inflammation, they would be vasodilation, cell migration, and, in the case of chronic inflam- tion, cell proliferation, often with an underlying autoimmune basis. Gen- ally, inflammation may be divided into acute, chronic, and autoimmune, - though the editors believe that most, if not all, chronic states are often the result of an autoimmune response to an endogenous antigen. Thus, a proper understanding of the inflammatory basis may provide clues to new therap- tic targets not only in classical inflammatory diseases, but atherosclerosis, cancer, and ischemic heart disease as well. The lack of advances in classical inflammatory diseases, such as rh- matoid arthritis, may in part arise from a failure to classify the disease into different forms. That different forms exist is exemplified in patients with d- fering responses to existing antiinflammatory drugs, ranging from nonresponders to very positive responders for a particular nonsteroidal an- inflammatory drug (NSAID). Though researchers have progressively unr- eled the mechanisms, the story is far from complete. It should also be noted that the inflammatory response is part of the innate immune response, or to use John Hunter’s words in 1795, “inflammation is a salutary response.” That may be applied in particular to the defensive response to invading micro- ganisms.

Matrix Metalloproteinase Biology

Irit Sagi,Jean Gaffney
Matrix Metalloproteinase Biology Book PDF

Author : Irit Sagi,Jean Gaffney
Publisher : John Wiley & Sons
Page : 232 pages
File Size : 41,7 Mb
Release : 2015-05-18
Category : Science
ISBN : 9781118772294

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Matrix Metalloproteinase Biology by Irit Sagi,Jean Gaffney Pdf

Discussing recent advances in the field of matrix metalloproteinase (MMP) research from a multidisciplinary perspective, Matrix Metalloproteinase Biologyis a collection of chapters written by leaders in the field of MMPs. The book focuses on the challenges of understanding the mechanisms substrate degradation by MMPs, as well as how these enzymes are able to degrade large, highly ordered substrates such as collagen. All topics addressed are considered in relation to disease progression including roles in cancer metastasis, rheumatoid arthritis and other inflammatory diseases. The text first provides an overview of MMPs, focusing on the history, the development and failures of small molecule inhibitors in clinical trials, and work with TIMPS, the endogenous inhibitors of MMPs. These introductory chapters establish the foundation for later discussion of the recent progress on the design of different types of inhibitors, including novel antibody based therapeutics. The following section emphasizes research using novel methods to further the study of the MMPs. The third and final section focuses on in vivo research, particularly with respect to cancer models, degradation of the extracellular matrix, and MMP involvement in other disease states. Written and edited by leaders in the field, Matrix Metalloproteinase Biology addresses the rapidly growth in MMP research, and will be an invaluable resource to advanced students and researchers studying cell and molecular biology.

The Role of Matrix Metalloproteinase in Human Body Pathologies

Francesco Travascio
The Role of Matrix Metalloproteinase in Human Body Pathologies Book PDF

Author : Francesco Travascio
Publisher : BoD – Books on Demand
Page : 202 pages
File Size : 48,8 Mb
Release : 2017-12-20
Category : Medical
ISBN : 9789535137177

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The Role of Matrix Metalloproteinase in Human Body Pathologies by Francesco Travascio Pdf

Matrix metalloproteinases (MMPs) are a family of proteolytic zinc-containing enzymes involved in physiological as well as in pathological processes in the human organism. MMPs play a key role in the remodeling of the extracellular matrix. Such a process may occur because of tissue homeostasis, morphogenesis, and tissue repair. However, remodeling could also be a part of many pathological states such as arthritis, cardiovascular diseases, neurodegenerative diseases, or impaired development in congenital anomalies. This book overviews the role of MMPs in different pathologies affecting the human body.

Arthritis Research

Andrew P. Cope
Arthritis Research Book PDF

Author : Andrew P. Cope
Publisher : Springer Science & Business Media
Page : 477 pages
File Size : 44,8 Mb
Release : 2008-02-02
Category : Medical
ISBN : 9781597454018

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Arthritis Research by Andrew P. Cope Pdf

This is a compendium of data pertinent to the methods and protocols that have contributed to recent advances in molecular medicine in general, but to the molecular basis of rheumatic disease in particular. These volumes details novel technologies, some of which are still evolving and whose impacts are yet to be determined. Leaders in the field contribute to cover exciting and cutting edge topics. This compendium will be a valuable tool.

Handbook of Proteolytic Enzymes

Alan J. Barrett,Neil D. Rawlings,J. Fred Woessner
Handbook of Proteolytic Enzymes Book PDF

Author : Alan J. Barrett,Neil D. Rawlings,J. Fred Woessner
Publisher : Academic Press
Page : 4094 pages
File Size : 54,6 Mb
Release : 2012-10-30
Category : Science
ISBN : 9780123822208

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Handbook of Proteolytic Enzymes by Alan J. Barrett,Neil D. Rawlings,J. Fred Woessner Pdf

The third edition of the Handbook of Proteolytic Enzymes is a comprehensive reference work for the enzymes that cleave proteins and peptides, written by acknowledged experts in the field and containing over 850 chapters. Each chapter is organized into sections describing the name and history, activity and specificity, structural chemistry, preparation, biological aspects, and distinguishing features for a specific peptidase. There are also introductory chapters on peptidase classification and mechanisms and a comprehensive index. For the first time, the Handbook is also available online via Elsevier's ScienceDirect platform as well as a three-volume book. The online version has enhanced options, including online multimedia, cross-referencing capabilities, integrated online delivery and closer integration with the online MEROPS database of peptidases and their inhibitors. This reference work is a must-have for biochemists, biotechnologists, molecular biologists and students in these disciplines, and will be of great interest to pharmaceutical and biotechnology companies. Contains over 830 chapters Covers new research in therapeutics and drug trials Supplies content written by experts in the field

Heart Failure: A Companion to Braunwald's Heart Disease E-book

Douglas L. Mann
Heart Failure A Companion to Braunwald s Heart Disease E book Book PDF

Author : Douglas L. Mann
Publisher : Elsevier Health Sciences
Page : 923 pages
File Size : 49,6 Mb
Release : 2010-11-11
Category : Medical
ISBN : 9781437703634

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Heart Failure: A Companion to Braunwald's Heart Disease E-book by Douglas L. Mann Pdf

Dr. Douglas L. Mann, one of the foremost experts in the field, presents the 2nd Edition of Heart Failure: A Companion to Braunwald’s Heart Disease. This completely reworked edition covers the scientific and clinical guidance you need to effectively manage your patients and captures the dramatic advances made in the field over the last five years. Now in full color, this edition features eleven new chapters, including advanced cardiac imaging techniques, use of biomarkers, cell-based therapies and tissue engineering, device therapies, and much more. Consult this title on your favorite e-reader, conduct rapid searches, and adjust font sizes for optimal readability. Compatible with Kindle®, nook®, and other popular devices. Use this Braunwald’s companion as the definitive source to prepare for the ABIM’s new Heart Failure board exam. Access the fully searchable contents of the book online at Expert Consult. This edition includes 67 new authors, who are experts in the field of heart failure Stay on the cutting edge with new chapters on: The latest practice guidelines for medical and device therapy Hemodynamic assessment of heart failure Contemporary medical therapy for heart failure patients with reduced and preserved ejection fraction Biomarkers in heart failure Pulmonary hypertension Management of co-morbidities in heart failure Mechanical cardiac support devices Get up to speed with the latest clinical trials, as well as how they have influenced current practice guidelines Explore what’s changing in key areas such as basic mechanisms of heart failure, genetic screening, cell and gene therapies, pulmonary hypertension, heart failure prevention, co-morbid conditions, telemedicine/remote monitoring, and palliative care

Cell Surface Proteases

Anonim
Cell Surface Proteases Book PDF

Author : Anonim
Publisher : Elsevier
Page : 452 pages
File Size : 54,6 Mb
Release : 2003-05-03
Category : Science
ISBN : 0080490883

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Cell Surface Proteases by Anonim Pdf

Cell Surface Proteases provides a comprehensive overview of these important enzymes that catalyze the hydrolysis of a protein as it degrades to a simpler substance. In the 1990s, an explosion of new discoveries shed light on the role of cell surface proteases and extended it beyond degradation of extracellular matrix components to include its influence on growth factors, cell signaling, and other cellular events. This volume unites the scientific literature from across disciplines and teases out unified themes of interactions between cell surface proteases and interconnecting cell surface-related systems -- including integrins and other adhesion molecules. Scientists and students involved in developmental biology, cell biology and disease processes will find this an indispensable resource. * Provides an overview of the entire field of cell surface proteases in a single volume * Presents major issues and astonishing discoveries at the forefront of modern developmental biology and developmental medicine * A thematic volume in the longest-running forum for contemporary issues in developmental biology with over 30 years of coverage

Heart Failure E-Book

G. Michael Felker,Douglas L. Mann
Heart Failure E Book Book PDF

Author : G. Michael Felker,Douglas L. Mann
Publisher : Elsevier Health Sciences
Page : 787 pages
File Size : 52,9 Mb
Release : 2014-12-18
Category : Medical
ISBN : 9780323315685

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Heart Failure E-Book by G. Michael Felker,Douglas L. Mann Pdf

Lead editor of Braunwald's Heart Disease, Dr. Douglas L. Mann, and nationally and internationally recognized heart failure expert Dr. G. Michael Felker, bring you the latest, definitive state-of-the art information on heart failure in this outstanding Braunwald's companion volume. Heart Failure, 3rd Edition keeps you current with recent developments in the field, improved patient management strategies, and new drug therapies and implantable devices that will make a difference in your patients' lives and your practice.

Advances in Clinical Chemistry

Anonim
Advances in Clinical Chemistry Book PDF

Author : Anonim
Publisher : Elsevier
Page : 266 pages
File Size : 49,5 Mb
Release : 2004-10-21
Category : Science
ISBN : 0080490271

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Advances in Clinical Chemistry by Anonim Pdf

Volume thirty-eight in the series of the internationally acclaimed Advances in Clinical Chemistry, contains chapters submitted from leading experts from academia and clinical laboratory science. Authors are from a diverse field of clinical chemistry disciplines and diagnostics ranging from basic biochemical exploration to cutting edge microarray technology. In keeping with the tradition of the series, this volume emphasizes novel laboratory advances with application not only to both clinical laboratory diagnostics, but as well as practical basic science studies. This volume of Advances in Clinical Chemistry is an indispensable resource and practical guide for twenty-first century practitioners of clinical chemistry, molecular diagnostics, pathology, and clinical laboratory sciences in general. Selected Contents on: -Biochemical markers in atherosclerosis and cancer -Molecular diagnostics in metastatic diseases -Single cell mRNA analysis -Protein detecting microarray technology -Evaluation of nerve agents and poisoning

Biologically Responsive Biomaterials for Tissue Engineering

Iulian Antoniac
Biologically Responsive Biomaterials for Tissue Engineering Book PDF

Author : Iulian Antoniac
Publisher : Springer Science & Business Media
Page : 254 pages
File Size : 47,7 Mb
Release : 2012-10-02
Category : Technology & Engineering
ISBN : 9781461443285

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Biologically Responsive Biomaterials for Tissue Engineering by Iulian Antoniac Pdf

Developments in the area of biomaterials, bionanotechnology, tissue engineering, and medical devices are becoming the core of health care. Almost all medical specialties involve the use of biomaterials, and research plays a key role in the development of new and improved treatment modalities. This volume focuses on several current trends in tissue engineering, remodelling and regeneration. Leading researchers describe the use of nanomaterials to create new functionalities when interfaced with biological molecules or structures. In addition to coverage of basic science and engineering aspects, a range of applications in bionanotechnology are presented, including diagnostic devices, contrast agents, analytical tools, physical therapy applications, and vehicles for targeted drug delivery. The use of polymers, alloys, and composites, or a combination of these, for biomaterials applications in orthopaedics is also explored. These contributions represent essential reading for the biomaterials and biomedical engineering communities, and can serve as instructional course lectures targeted at graduate and post-graduate students.

Zymography

Jeff Wilkesman,Liliana Kurz
Zymography Book PDF

Author : Jeff Wilkesman,Liliana Kurz
Publisher : Humana
Page : 310 pages
File Size : 44,5 Mb
Release : 2018-06-12
Category : Science
ISBN : 1493983873

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Zymography by Jeff Wilkesman,Liliana Kurz Pdf

This detailed volume examines the latest techniques and protocols associated with zymography, the practice of detecting enzyme activity on electrophoretic gels. Sections cover subjects such as endopeptidase zymography, reverse zymography & in situ zymography, 2D zymography, as well as a variety of special cases. Written in the highly respected Methods in Molecular Biology series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and practical, Zymography: Methods and Protocols serves as an ideal aid for a broad array of researchers from the fields of biological and medicinal science, as well as scientists working in clinical and diagnostic medicine, medical genetics, agricultural entomology, genetic monitoring of environmental pollution, and forensic science.

Capillary Electrophoresis of Nucleic Acids

Keith R. Mitchelson,Jing Cheng
Capillary Electrophoresis of Nucleic Acids Book PDF

Author : Keith R. Mitchelson,Jing Cheng
Publisher : Springer Science & Business Media
Page : 481 pages
File Size : 54,5 Mb
Release : 2008-02-05
Category : Science
ISBN : 9781592590551

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Capillary Electrophoresis of Nucleic Acids by Keith R. Mitchelson,Jing Cheng Pdf

The development of PCR, which enables extremely small amounts of DNA to be amplified, led to the rapid development of a multiplicity of a- lytical procedures that permit use of this new resource for the analysis of genetic variation and for the detection of disease-causing mutations. The advent of capillary electrophoresis (CE), with its power to separate and a- lyze very small amounts of DNA, has also stimulated researchers to develop analytical procedures for the CE format. The advantages of CE in terms of speed and reproducibility of analyses are manifold. Furthermore, the high s- sitivity of detection, and the ability to increase sample throughput with par- lel analysis, has led to the creation of a full range of analysis of DNA molecules, from modified DNA adducts and single-strand oligonucleotides through PCR-amplified DNA fragments and whole chromosomes. Capillary Elect- phoresis of Nucleic Acids focuses on analytical protocols that can be used for detection and analysis of mutations and modification, from precise DNA loci through entire genomes of organisms. Important practical considerations for CE, such as the choice of separation media, electrophoresis conditions, and the influence of buffer additives and dyes on DNA mobility, are discussed in several key chapters and within particular applications.