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Methods in Nitric Oxide Research by Martin Feelisch,Jonathan S. Stamler Pdf
This handbook describes methods for the measurement and investigation of nitric oxide. The topics discussed include the metabolism of substrates and cofactors, the detection of nitrogen oxides, the detection of nitrogen oxide synthase activity, and the detection of adducts and reaction products.
Nitric Oxide by Helen O. McCarthy,Jonathan A. Coulter Pdf
The past two decades have seen an explosion in the number of research articles relating to both the physiological and pathological responses evoked by nitric oxide generation. Despite this, accurate quantification of nitric oxide in either in vitro or in vivo models remains challenging, due to the relatively unstable nature of the molecule. In Nitric Oxide: Methods and Protocols, experts in the field provide a detailed reference manual packed with step-by-step approaches to reproduce various complex protocols within your own laboratory. Divided into two parts, the volume first covers various disparate protocols relating to the direct detection and quantification of nitric oxide, including techniques which detail how to image real time in vivo generation of nitric oxide, quantify nitric oxide production in the rat brain, and the detect ultralow levels of nitric oxide in the pM range, and the book then continues with a section on techniques designed to either inhibit or enhance nitric oxide, with an aim to achieve therapeutic gain, featuring the inhibition of the nitric oxide synthase enzymes using viral, shRNA delivery systems to prevent cardiovascular dysfunction, peripheral neuropathy, and graft rejection. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and easy to use, Nitric Oxide: Methods and Protocols examines numerous complex methodologies detailed in each chapter and proves to be an invaluable resource for anyone with an interest in nitric oxide research.
With numerous methods available for the quantification of nitric oxide (NO), this detailed book explores their advantages and disadvantages in order to provide a foundation for further research in plant nitric oxide. After providing a useful practical guide to choosing a technique for measuring NO from plant materials, the book continues with chapters on chemiluminescence, diaminofluorescence (DAF), EPR spectroscopy, a laser-based method, as well as many other topics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory methods, and tips on troubleshooting and avoiding known pitfalls. Practical and authoritative, Plant Nitric Oxide: Methods and Protocols serves as a valuable guide to all researchers working and intending to work in the field of plant nitric oxide research.
Methods in Enzymology by Lester Packer,Enrique Cadenas Pdf
Since the inception of the series, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today - truly an essential publication for researchers in all field of life sciences. This final volume in the five-part Nitric Oxide series supplements MIE volumes 268, 269, 301 and 359. Nitric Oxide impinges on a wide range of fields in biological research, particularly in the areas of biomedicine and cell and organic biology, as well as fundamental chemistry. These volumes are a valuable resource for the experienced researcher and for those entering the field. *One of the most highly respected publication in the field of biochemistry since 1955 *Frequently consulted and praised by researchers and reviewers alike *Truly an essential publication for anyone in any field of the life sciences
Carrying on the high standards of the much-praised first edition of Nitric Oxide Protocols, Aviv Hassid has brought together a panel of expert researchers and clinician scientists to describe in step-by-step detail the latest methodologies for the measurement of nitric oxide--and the enzyme that produces it-in biological tissues and fluids. The authors take advantage of the latest methodologies for the quantitation of biological fluids and tissues, including capillary electrophoresis, microcoaxial electrodes, in vivo measurement of nitric oxide in exhaled air, confocal microscopy, gas chromatography, in situ hybridization, and real-time polymerase chain reaction. Chapters on the measurement of the novel products of nitric oxide, such as nitrated proteins, S-nitrosylated proteins, and dioxygen-dependent NO metabolism, are also included. Additional chapters address the expression of nitric oxide synthase via the use of viral vectors in gene therapy for erectile dysfunction and cancer, as well as in retrovirus, adenovirus, or adenoassociated virus-mediated expression of nitric oxide synthase in vivo. The protocols follow the successful Methods in Molecular BiologyTM series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. State-of-the-art and highly practical, Nitric Oxide Protocols, 2nd ed., offers investigators and clinican/scientists a gold-standard collection of readily reproducible analytical techniques for measuring levels of nitric oxide and determining its manifold functions and effects.
Nitric Oxide in Health and Disease by Jill Lincoln,Charles H. V. Hoyle,Geoffrey Burnstock Pdf
Nitric oxide has a tantalizing role in health and disease: while many of its wide-ranging effects are well known, there remains much more to explore and to learn about the interactions of this fascinating molecule in physiological and pathophysiological processes. The volume reviews the myriad effects of nitric oxide as a chemical messenger in the central nervous system, peripheral nervous system, immune system and cardiovascular system. Furthermore, it provides a very practical introduction to the procedures and experimental protocols necessary to work with and study nitric oxide and its synthesizing enzyme, nitric oxide synthase, in the laboratory. In this respect the volume is unique, providing as it does a complete single-volume review of the role of nitric oxide in health and disease, and a very practical introduction to the methods and protocols involved in this intriguing and active area of biomedical research.
Nitric oxide (NO) is a gas that transmits signals in an organism. Signal transmission by a gas that is produced by one cell and which penetrates through membranes and regulates the function of another cell represents an entirely new principle for signaling in biological systems. NO is a signal molecule of key importance for the cardiovascular system acting as a regulator of blood pressure and as a gatekeeper of blood flow to different organs. NO also exerts a series of other functions, such as acting a signal molecule in the nervous system and as a weapon against infections. NO is present in most living creatures and made by many different types of cells. NO research has led to new treatments for treating heart as well as lung diseases, shock, and impotence. Scientists are currently testing whether NO can be used to stop the growth of cancerous tumors, since the gas can induce programmed cell death, apoptosis. This book is the first comprehensive text on nitric oxide to cover all aspects--basic biology, chemistry, pathobiology, effects on various disease states, and therapeutic implications. Edited by Nobel Laureate Louis J. Ignarro, editor of the Academic Press journal, Nitric Oxide Authored by world experts on nitric oxide Includes an overview of basic principles of biology and chemical biology Covers principles of pathobiology, including the nervous system, cardiovascular function, pulmonary function, and immune defense
General Description of the Volume: Nitric Oxide, recently designated "Molecule of the Year," impinges on a wide range of fields in biological research, particularly in the areas of biomedicine and cell and organismal biology, as well as in fundamental chemistry. This volume will be a valuable resource for the experienced researcher as well as for those newly entering the field. This volume continues the coverage of new and important tools for the elucidation of Nitric Oxide action initiated in Volumes 268 and 269 of Methods in Enzymology. Techniques for researching the physiology and toxicity of nitric oxide in cellular and organismal systems are highlighted. General Description of the Series: The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences. Key Features * Biological Activity * NO Donors: Nitrosothiols and Nitroxyls * Peroxynitrite * Oxidant and Antioxidant Action.
Nitric Oxide, Part A: Sources and Detection of NO; NO Synthase by Anonim Pdf
Nitric oxide, recently named"Molecule of the Year"by the journal Science, impinges on a wide range of fields in biological research, particularly in the areas of biomedicine and cell and organismal biology, as well as in fundamental chemistry. This volume and its companion Volume 269 highlight the most current and up-to-date methods used to research the physiology and toxicity of nitric oxide in cellular and organismal systems. It will be a valuable resourcefor the experienced researcher as well as for those newly entering the field. Generation, detection, and characterization of biological and chemical sources of nitric oxide Biochemistry and molecular biology of enzymes and proteins associated with nitric oxide metabolism
Nitric Oxide Synthase: Characterization and Functional Analysis by Anonim Pdf
Although the function of nitric oxide in a regulatory capacity in the central and peripheral nervous system is widely recognized, the full scope of its actions and its interrelationships with other classes of regulatory molecules is just beginning to be comprehended. This volume contains a number of sophisticated and advanced methods essential for exploring the activity of nitric oxide in the brain. It will be a valuable tool for the established investigator and for those just entering the field. Comprehensive protocols included for detection of NO and related compounds by chemical, immunohistochemical, and in situ hybridization techniques Newly developed methods for the purification of neuronal and endothelial NO synthase, production of monoclonal antibodies to NO synthase, molecular cloning and expression of NO synthesis, and control of NO synthase gene expression Assessment of NO-mediated functions in neurons, central nervous system, cerebral circulation, synaptic transmission, and vascular tone Calcium imaging by confocal microscopy, evaluation of the effect of NO on iron metabolism, and detection of heme oxygenase-1 and -2 message level and distribution
The Nobel Prize was awarded in Physiology or Medicine in 1998 to Louis J. Ignarro, Robert F. Furchgott and Ferid Murad for demonstrating the signaling properties of nitric oxide. Nitric oxide (NO) is one of the few gaseous signaling molecules and is a key biological messenger that plays a role in many biological processes. NO research has led to new treatments for treating heart as well as lung diseases, shock and impotence. (Sildenafil, popularly known by the trade name Viagra, enhances signaling through NO pathways.) Scientists are currently testing whether NO can be used to stop the growth of cancerous tumors, since the gas can induce programmed cell death, apoptosis.This is another “must-have volume packed with robust methods from authors around the globe. Researchers interested in the detailed biochemistry of NO and its synthesis will have this indispensable volume on their shelves. Essential resource for every laboratory involved in NO-related work Gathers tried and tested techniques from global labs which eliminates searching through many different sources and avoids pitfalls so the same mistakes are not made over and over Aids researchers in the design of medically important therapies for heart disease and cancer
Nitric Oxide, Part B: Physiological and Pathological Processes by Anonim Pdf
Nitric oxide, recently named"Molecule of the Year"by the journal Science, impinges on a wide range of fields in biological research, particularly in the areas of biomedicine and cell and organismal biology, as well as in fundamental chemistry. This volume and its companion Volume 268 highlight methods used to research the physiology and toxicity of nitric oxide in cellular and organismal systems. It will be a valuable resource for the experienced researcher as well as for those newly entering the field.
