Methods Of Cell Separation

Author : P.T. Sharpe
Publisher : Elsevier
Page : 286 pages
File Size : 49,8 Mb
Release : 1988-05-01
Category : Science
ISBN : 0080858872

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Methods of Cell Separation by P.T. Sharpe Pdf

Methods of Cell Separation brings to the attention of researchers at all levels the variety of methods available for separating viable populations of cells. Methods are grouped into 3 categories based on the criteria of separation, namely; size or density; non-specific surface properties; and specific surface properties. The principle of each method is described together with general and, where possible, specific protocols for conducting cell separation experiments. A central theme of the book is the separation of populations of blood lymphocytes which is used as an example for each method. Methods of Cell Separation is distinguished by three powerful assets: descriptions of the majority of cell separation methods currently being used; details of the experimental procedures involved in each method; and comparisons of the different methods for separating cell populations with particular reference to blood lymphocytes. An excellent addition to a distinguished series, and extremely useful as a laboratory manual.

Author : Diether Recktenwald
Publisher : CRC Press
Page : 354 pages
File Size : 54,7 Mb
Release : 1997-11-04
Category : Medical
ISBN : 0824798643

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Cell Separation Methods and Applications by Diether Recktenwald Pdf

"Offers complete coverage and assessment of cell separation technologies for analytical and preparative isolations of biological cell populations-demonstrating how to select and devise optimal sorting strategies for applications in biochemistry, immunology, cell and molecular biology, and clinical research. "

Author : Thomas G. Pretlow,Theresa P. Pretlow
Publisher : Academic Press
Page : 336 pages
File Size : 44,9 Mb
Release : 2014-05-10
Category : Science
ISBN : 9781483219400

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Cell Separation by Thomas G. Pretlow,Theresa P. Pretlow Pdf

Cell Separation: Methods and Selected Application, Volume 3 provides information pertinent to the design and application of methods for the separation of cells. This book covers a variety of topics, including lymphoma cells, lectins, purification of cells from lung tumors, macrophage electrophoretic migration test, tissue heterogeneity, and characteristics of cultured cells. Organized into 13 chapters, this volume begins with an overview of the approaches in examining particular cell-surface properties and their role in the metastatic process. This text then examines lectins as important tools for identification and separation of cells, particularly of lymphocyte subpopulations. Other chapters consider the various methods that have been used to disperse rat pituitary tissue into single cells. This book discusses as well the different methods for isolating type II cells. The final chapter deals with the significance of having cell cultures homogeneous for a given cell type. This book is a valuable resource for cell biologists, experimental oncologists, hematologists, immunologists, and endocrinologists.

Author : Nicholas Catsimpoolas
Publisher : Springer Science & Business Media
Page : 211 pages
File Size : 51,6 Mb
Release : 2013-03-08
Category : Medical
ISBN : 9781468436624

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Methods of Cell Separation by Nicholas Catsimpoolas Pdf

Presently, the need for methods involving separation, identification, and characterization of different kinds of cells is amply realized among immu nologists, hematologists, cell biologists, clinical pathologists, and cancer researchers. Unless cells exhibiting different functions and stages of differ entiation are separated from one another, it will be exceedingly difficult to study some of the molecular mechanisms involved in cell recognition, spe cialization, interactions, cytotoxicity, and transformation. Clinical diagno sis of diseased states and use of isolated cells for therapeutic (e. g. , immu notherapy) or survival (e. g, transfusion) purposes are some of the pressing areas where immediate practical benefits can be obtained by applying cell separation techniques. However, the development of such useful methods is still in its infancy. A number of good techniques exist based either on the physical or biological properties of the cells, and these have produced some valuable results. Still others are to be discovered. Therefore, the purpose of this open-ended treatise is to acquaint the reader with some of the basic principles, instrumentation, and procedures presently in practice at various laboratories around the world and to present some typical applications of each technique to particular biological problems.

Author : Nicholas Catsimpoolas
Publisher : Unknown
Page : 220 pages
File Size : 52,9 Mb
Release : 1980-05-01
Category : Electronic
ISBN : 1468436635

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Methods of Cell Separation by Nicholas Catsimpoolas Pdf

Author : Paul T. Sharpe
Publisher : Unknown
Page : 272 pages
File Size : 41,7 Mb
Release : 1988
Category : Cell separation
ISBN : 0720442001

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Methods of Cell Separation by Paul T. Sharpe Pdf

Author : Ashok Kumar,Igor Yu Galaev,Bo Mattiasson
Publisher : Springer
Page : 203 pages
File Size : 42,6 Mb
Release : 2007-10-09
Category : Science
ISBN : 9783540752639

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Cell Separation by Ashok Kumar,Igor Yu Galaev,Bo Mattiasson Pdf

With contributions by numerous experts

Author : Anonim
Publisher : Elsevier
Page : 486 pages
File Size : 44,8 Mb
Release : 2011-08-31
Category : Science
ISBN : 0080553508

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Magnetic Cell Separation by Anonim Pdf

Cell separation is at the core of current methods in experimental biology and medicine. Its importance is illustrated by the large number of physical and biochemical principles that have been evaluated for application to cell separation. The development of cell separation methods is driven by the needs of biological and medical research, and the ever-increasing demands for sensitivity, selectivity, yield, timeliness and economy of the process. The interdisciplinary nature of research in this area and the volume of information available in research publications and conferences necessitates a basic description of the fundamental processes involved in magnetic cell separation that may help the user in navigating this wealth of information available online and in scientific publications. This book will appeal to researchers in many areas utilizing this technique, including those working in cell biology, clinical research, inorganic chemistry, biochemistry, chemical engineering, materials science, physics and electrical engineering. Provides examples of how to calculate the volume magnetic susceptibility, a fundamental quantity for calculating the magnetic force acting on a cell, from various types of magnetic susceptibilities available in literature Introduces the elements of magnetostatics as they apply to cell magnetization and the magnetization of magnetic micro- and nano- particles used for cell separation Describes the parameters used to determine cell magnetophoresis

Author : Bruce Alberts
Publisher : Unknown
Page : 0 pages
File Size : 54,6 Mb
Release : 2002
Category : Cytology
ISBN : 0815332181

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Molecular Biology of The Cell by Bruce Alberts Pdf

Author : Derek Fisher,Gillian E. Francis,David Rickwood
Publisher : OUP Oxford
Page : 290 pages
File Size : 40,5 Mb
Release : 1998-11-26
Category : Science
ISBN : 9780191565557

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Cell Separation by Derek Fisher,Gillian E. Francis,David Rickwood Pdf

Techniques for separating cells are needed in many areas of cell biology. This book presents modern methods from the laboratories of experts in the field, and includes tested, reproducible protocols, hints and tips for success, and troubleshooting suggestions. It will be invaluable to a wide range of cell biologists.

Author : Thomas G. Pretlow
Publisher : Unknown
Page : 330 pages
File Size : 43,6 Mb
Release : 1982
Category : Cell separation
ISBN : OCLC:38054356

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Cell separation : methods and selected applications. 1 (1982) by Thomas G. Pretlow Pdf

Author : A. M. Cheret
Publisher : Unknown
Page : 400 pages
File Size : 43,7 Mb
Release : 1982
Category : Cell separation
ISBN : UOM:39015018296460

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Cell Separation by A. M. Cheret Pdf

Author : Gillian, Derek Francis Fisher
Publisher : Oxford University Press, USA
Page : 267 pages
File Size : 51,5 Mb
Release : 1998
Category : Science
ISBN : 1280375396

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Cell Separation by Gillian, Derek Francis Fisher Pdf

Isolating specific cells or fractionating viable cell populations is an essential step of many of the techniques used across a wide range of biological and related disciplines. This text aims to inform those working in all areas of the biological sciences of the methods which are available to them and which is likely to be the most useful.; The contributing authors have provided a comprehensive guide to the methods used to prepare cell suspensions and to separate viable cells depending on their different characteristics - such as size, density, surface charge and immunological identity. Each chapter offers direct practical guidance for the various techniques and describes the advantages and limitations of each. Reproducible protocols, hints and tips for success and troubleshooting suggestions are provided.

Author : J. Harry Cutts
Publisher : Unknown
Page : 248 pages
File Size : 40,8 Mb
Release : 1970
Category : Science
ISBN : UOM:39015004434117

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Cell Separation by J. Harry Cutts Pdf

Author : Anonim
Publisher : Elsevier
Page : 486 pages
File Size : 49,9 Mb
Release : 2011-08-31
Category : Science
ISBN : 0080553508

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Magnetic Cell Separation by Anonim Pdf

Cell separation is at the core of current methods in experimental biology and medicine. Its importance is illustrated by the large number of physical and biochemical principles that have been evaluated for application to cell separation. The development of cell separation methods is driven by the needs of biological and medical research, and the ever-increasing demands for sensitivity, selectivity, yield, timeliness and economy of the process. The interdisciplinary nature of research in this area and the volume of information available in research publications and conferences necessitates a basic description of the fundamental processes involved in magnetic cell separation that may help the user in navigating this wealth of information available online and in scientific publications. This book will appeal to researchers in many areas utilizing this technique, including those working in cell biology, clinical research, inorganic chemistry, biochemistry, chemical engineering, materials science, physics and electrical engineering. Provides examples of how to calculate the volume magnetic susceptibility, a fundamental quantity for calculating the magnetic force acting on a cell, from various types of magnetic susceptibilities available in literature Introduces the elements of magnetostatics as they apply to cell magnetization and the magnetization of magnetic micro- and nano- particles used for cell separation Describes the parameters used to determine cell magnetophoresis