Microfluidic Methods For Molecular Biology

Microfluidic Methods For Molecular Biology Book in PDF, ePub and Kindle version is available to download in english. Read online anytime anywhere directly from your device. Click on the download button below to get a free pdf file of Microfluidic Methods For Molecular Biology book. This book definitely worth reading, it is an incredibly well-written.

Microfluidic Methods for Molecular Biology

Chang Lu,Scott S. Verbridge
Microfluidic Methods for Molecular Biology Book PDF

Author : Chang Lu,Scott S. Verbridge
Publisher : Springer
Page : 376 pages
File Size : 55,8 Mb
Release : 2016-05-14
Category : Medical
ISBN : 9783319300191

Get Book

Microfluidic Methods for Molecular Biology by Chang Lu,Scott S. Verbridge Pdf

This book covers the state-of-the-art research on molecular biology assays and molecular techniques enabled or enhanced by microfluidic platforms. Topics covered include microfluidic methods for cellular separations and single cell studies, droplet-based approaches to study protein expression and forensics, and microfluidic in situ hybridization for RNA analysis. Key molecular biology studies using model organisms are reviewed in detail. This is an ideal book for students and researchers in the microfluidics and molecular biology fields as well as engineers working in the biotechnology industry. This book also: Reviews exhaustively the latest techniques for single-cell genetic, epigenetic, metabolomic, and proteomic analysis Illustrates microfluidic approaches for inverse metabolic engineering, as well as analysis of circulating exosomes Broadens readers’ understanding of microfluidics convection-based PCR technology, microfluidic RNA-seq, and microfluidics for robust mobile diagnostics

Microfluidic Techniques

Shelley D. Minteer
Microfluidic Techniques Book PDF

Author : Shelley D. Minteer
Publisher : Springer Science & Business Media
Page : 245 pages
File Size : 51,9 Mb
Release : 2008-02-04
Category : Science
ISBN : 9781592599974

Get Book

Microfluidic Techniques by Shelley D. Minteer Pdf

Hands-on researchers review the principles behind successful miniaturization and describe the key techniques for miniaturizing large-scale biochemical and bioanalytical methods for microchip analysis. The authors cover not only the most popular methods for the fabrication of microchips (photolithography, laser ablation, and soft lithography), but also microfluidic techniques for such bioanalytical assays and bioprocesses as DNA analysis, PCR, immunoassays, and cell reactors. Highlights include PCR on a microchip, microscale cell culturing, and the study of cellular processes on a microchip. The protocols offer step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

Microfluidics for Biologists

Chandra K. Dixit,Ajeet Kaushik
Microfluidics for Biologists Book PDF

Author : Chandra K. Dixit,Ajeet Kaushik
Publisher : Springer
Page : 252 pages
File Size : 48,6 Mb
Release : 2016-10-13
Category : Medical
ISBN : 9783319400365

Get Book

Microfluidics for Biologists by Chandra K. Dixit,Ajeet Kaushik Pdf

This book describes novel microtechnologies and integration strategies for developing a new class of assay systems to retrieve desired health information from patients in real-time. The selection and integration of sensor components and operational parameters for developing point-of-care (POC) are also described in detail. The basics that govern the microfluidic regimen and the techniques and methods currently employed for fabricating microfluidic systems and integrating biosensors are thoroughly covered. This book also describes the application of microfluidics in the field of cell and molecular biology, single cell biology, disease diagnostics, as well as the commercially available systems that have been either introduced or have the potential of being used in research and development. This is an ideal book for aiding biologists in understanding the fundamentals and applications of microfluidics. This book also: Describes the preparatory methods for developing 3-dimensional microfluidic structures and their use for Lab-on-a-Chip design Explains the significance of miniaturization and integration of sensing components to develop wearable sensors for point-of-care (POC) Demonstrates the application of microfluidics to life sciences and analytical chemistry, including disease diagnostics and separations Motivates new ideas related to novel platforms, valving technology, miniaturized transduction methods, and device integration to develop next generation sequencing Discusses future prospects and challenges of the field of microfluidics in the areas of life sciences in general and diagnostics in particular

Microfluidics in Cell Biology Part C: Microfluidics for Cellular and Subcellular Analysis

Anonim
Microfluidics in Cell Biology Part C Microfluidics for Cellular and Subcellular Analysis Book PDF

Author : Anonim
Publisher : Academic Press
Page : 266 pages
File Size : 42,5 Mb
Release : 2018-11-22
Category : Science
ISBN : 9780128142851

Get Book

Microfluidics in Cell Biology Part C: Microfluidics for Cellular and Subcellular Analysis by Anonim Pdf

Microfluidics in Cell Biology Part C, Volume 148, a new release in the Methods in Cell Biology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Unique to this updated volume are three sections on microfluidics in various multi-cellular models, including microfluidics in cell monolayers/spheroids, microfluidics in organ on chips, and microfluidics in model organisms. Specific chapters discuss collective migration in microtubes, leukocyte adhesion dynamics on endothelial monolayers under flow, constrained spheroid for perfusion culture, cells in droplet arrays, heart on chips, kidney on chips, liver on chips, and more. Contains contributions from experts in the field from across the world Covers a wide array of topics on both mitosis and meiosis Includes relevant, analysis based topics

Microfluidic Diagnostics

Gareth Jenkins,Colin David Mansfield
Microfluidic Diagnostics Book PDF

Author : Gareth Jenkins,Colin David Mansfield
Publisher : Unknown
Page : 525 pages
File Size : 43,8 Mb
Release : 2013
Category : Biotechnology
ISBN : 1627031340

Get Book

Microfluidic Diagnostics by Gareth Jenkins,Colin David Mansfield Pdf

Microfluidic techniques are becoming widely incorporated into medical diagnostic systems due to the inherent advantages of miniaturization. In Microfluidic Diagnostics: Methods in Molecular Biology, researchers in the field detail methods and protocols covering subjects such as microfluidic device fabrication, on-chip sample preparation, diagnostic applications and detection methodologies. The protocols described range from cutting-edge developments to established techniques and basic demonstrations suitable for education and training; from basic fabrication methods to commercializing research. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Microfluidic Diagnostics: Methods in Molecular Biology seeks to aid scientists in the further development and commercialization of microfluidic diagnostic technologies.

Microfluidics in Cell Biology Part B: Microfluidics in Single Cells

Anonim
Microfluidics in Cell Biology Part B Microfluidics in Single Cells Book PDF

Author : Anonim
Publisher : Academic Press
Page : 247 pages
File Size : 40,7 Mb
Release : 2018-08-27
Category : Science
ISBN : 9780128142837

Get Book

Microfluidics in Cell Biology Part B: Microfluidics in Single Cells by Anonim Pdf

Microfluidics in Cell Biology Part B: Microfluidics in Single Cells, Volume 147, a new volume in the Methods in Cell Biology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Unique to this updated volume are three sections on microfluidics in various single cell models, including microfludics in micro-organisms, microfluidics for cell culture and cell sorting of mammalian cells, and microfluidics for cell migration. Specific sections in this latest release include Temperature control and drug delivery for cell division cycle control in fission yeast H2O2 stress response in budding yeast, Antibiotic resistance in bacteria, Metabolism in bacteria, Fluidized beds for bacterial sorting and amplification, Microfluidics for cell culture and cell sorting of mammalian cells, Hydrogel microwells, Immune cells migration in complex environments, Neutrophiles migration in health and disease, Cell guidance by physical cues, Stable gradients in gels of extracellular matrix for cancer cell migration, and more. Contains contributions from experts in the field from across the world Covers a wide array of topics on both mitosis and meiosis Includes relevant, analysis based topics

Microfluidics in Cell BiologyPart A: Microfluidics for multicellular systems

Anonim
Microfluidics in Cell BiologyPart A Microfluidics for multicellular systems Book PDF

Author : Anonim
Publisher : Academic Press
Page : 273 pages
File Size : 45,7 Mb
Release : 2018-07-20
Category : Science
ISBN : 9780128142813

Get Book

Microfluidics in Cell BiologyPart A: Microfluidics for multicellular systems by Anonim Pdf

Microfluidics in Cell Biology Part A: Volume 146, the latest release in the Methods in Cell Biology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Unique to this updated volume are sections on Cell monolayers/spheroids, Collective migration in microtubes, Leukocyte adhesion dynamics on endothelial monolayers under flow, Constrained spheroid for perfusion culture, Cells in droplet arrays, Heart on chips, Kidney on chips, Liver on chips and hepatic immune responses, Gut on chips, 3D microvascular model-based lymphoma model, Blood brain barrier on chips, Multi-organ-on-a-chip for pharmacokinetic analysis, Cancer immunotherapy on chips, and more. Contains contributions from experts in the field from across the globe Covers a wide array of topics on both mitosis and meiosis Includes relevant, analysis based topics

Optimization of Trustworthy Biomolecular Quantitative Analysis Using Cyber-Physical Microfluidic Platforms

Mohamed Ibrahim,Krishnendu Chakrabarty
Optimization of Trustworthy Biomolecular Quantitative Analysis Using Cyber Physical Microfluidic Platforms Book PDF

Author : Mohamed Ibrahim,Krishnendu Chakrabarty
Publisher : CRC Press
Page : 335 pages
File Size : 51,8 Mb
Release : 2020-05-31
Category : Technology & Engineering
ISBN : 9781000082708

Get Book

Optimization of Trustworthy Biomolecular Quantitative Analysis Using Cyber-Physical Microfluidic Platforms by Mohamed Ibrahim,Krishnendu Chakrabarty Pdf

A microfluidic biochip is an engineered fluidic device that controls the flow of analytes, thereby enabling a variety of useful applications. According to recent studies, the fields that are best set to benefit from the microfluidics technology, also known as lab-on-chip technology, include forensic identification, clinical chemistry, point-of-care (PoC) diagnostics, and drug discovery. The growth in such fields has significantly amplified the impact of microfluidics technology, whose market value is forecast to grow from $4 billion in 2017 to $13.2 billion by 2023. The rapid evolution of lab-on-chip technologies opens up opportunities for new biological or chemical science areas that can be directly facilitated by sensor-based microfluidics control. For example, the digital microfluidics-based ePlex system from GenMarkDx enables automated disease diagnosis and can bring syndromic testing near patients everywhere. However, as the applications of molecular biology grow, the adoption of microfluidics in many applications has not grown at the same pace, despite the concerted effort of microfluidic systems engineers. Recent studies suggest that state-of-the-art design techniques for microfluidics have two major drawbacks that need to be addressed appropriately: (1) current lab-on-chip systems were only optimized as auxiliary components and are only suitable for sample-limited analyses; therefore, their capabilities may not cope with the requirements of contemporary molecular biology applications; (2) the integrity of these automated lab-on-chip systems and their biochemical operations are still an open question since no protection schemes were developed against adversarial contamination or result-manipulation attacks. Optimization of Trustworthy Biomolecular Quantitative Analysis Using Cyber-Physical Microfluidic Platforms provides solutions to these challenges by introducing a new design flow based on the realistic modeling of contemporary molecular biology protocols. It also presents a microfluidic security flow that provides a high-level of confidence in the integrity of such protocols. In summary, this book creates a new research field as it bridges the technical skills gap between microfluidic systems and molecular biology protocols but it is viewed from the perspective of an electronic/systems engineer.

Microfluidics for Biological Applications

Wei-Cheng Tian,Erin Finehout
Microfluidics for Biological Applications Book PDF

Author : Wei-Cheng Tian,Erin Finehout
Publisher : Springer Science & Business Media
Page : 429 pages
File Size : 47,5 Mb
Release : 2009-03-02
Category : Technology & Engineering
ISBN : 9780387094809

Get Book

Microfluidics for Biological Applications by Wei-Cheng Tian,Erin Finehout Pdf

Microfluidics for Biological Applications provides researchers and scientists in the biotechnology, pharmaceutical, and life science industries with an introduction to the basics of microfluidics and also discusses how to link these technologies to various biological applications at the industrial and academic level. Readers will gain insight into a wide variety of biological applications for microfluidics. The material presented here is divided into four parts, Part I gives perspective on the history and development of microfluidic technologies, Part II presents overviews on how microfluidic systems have been used to study and manipulate specific classes of components, Part III focuses on specific biological applications of microfluidics: biodefense, diagnostics, high throughput screening, and tissue engineering and finally Part IV concludes with a discussion of emerging trends in the microfluidics field and the current challenges to the growth and continuing success of the field.

Micro/Nanofluidics and Lab-on-Chip Based Emerging Technologies for Biomedical and Translational Research Applications - Part B

Anonim
Micro Nanofluidics and Lab on Chip Based Emerging Technologies for Biomedical and Translational Research Applications Part B Book PDF

Author : Anonim
Publisher : Academic Press
Page : 370 pages
File Size : 47,5 Mb
Release : 2022-01-28
Category : Science
ISBN : 9780323853040

Get Book

Micro/Nanofluidics and Lab-on-Chip Based Emerging Technologies for Biomedical and Translational Research Applications - Part B by Anonim Pdf

Micro/Nanofluidics and Lab-on-Chip Based Emerging Technologies for Biomedical and Translational Research Applications - Part B, Volume 187 represents the collation of chapters written by eminent scientists worldwide. Chapters in this new release include Design and fabrication of microfluidics devices for molecular biology applications, Micro/Nanofluidics devices for drug delivery, From organ-on-chip to body-on-chip: the next generation of microfluidics platforms for in vitro drug toxicity testing, Micro/Nanofluidics for high throughput drug screening, Design, fabrication and assembly of lab-on-a-chip and its uses, Advances in microfluidic 3D cell culture for pre-clinical drug development, Tissue and organ culture on lab-on-a chip for biomedical applications, and much more. Offers a basic understanding of the state-of-the-art design and fabrication of microfluidics/ nanofluidics and lab on chip Explains how to develop microfluidics/nanofluidic for advanced application such as healthcare, high throughout drug screening, 3D cell culture and organ-on-chip Discusses the emerging demands and research of micro/nanofluidic based devices in biomedical and translational research applications

Microchip Diagnostics

Valérie Taly,Jean-Louis Viovy,Stéphanie Descroix
Microchip Diagnostics Book PDF

Author : Valérie Taly,Jean-Louis Viovy,Stéphanie Descroix
Publisher : Humana
Page : 223 pages
File Size : 46,7 Mb
Release : 2018-06-08
Category : Science
ISBN : 1493982869

Get Book

Microchip Diagnostics by Valérie Taly,Jean-Louis Viovy,Stéphanie Descroix Pdf

This volume illustrates how microfluidic approaches can meet the requirement of clinical diagnosis based on molecular or cellular biomarkers. Microchip Diagnostics: Methods and Protocols is divided into four sections describing the business aspects of the microfluidic-based systems for diagnosis, demonstrating how versatile microfluidics can be regarding to protein bioassay integration, presenting microfluidic approaches for nucleic analysis based on mono or diphasic format, and highlighting recent contributions. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cell Analysis on Microfluidics

Jin-Ming Lin
Cell Analysis on Microfluidics Book PDF

Author : Jin-Ming Lin
Publisher : Springer
Page : 429 pages
File Size : 43,9 Mb
Release : 2017-10-25
Category : Science
ISBN : 9789811053948

Get Book

Cell Analysis on Microfluidics by Jin-Ming Lin Pdf

This book presents a detailed overview of the design, formatting, application, and development of microfluidic chips in the context of cell biology research, enumerating each element involved in microfluidics-based cell analysis, discussing its history, status quo, and future prospects, It also offers an extensive review of the research completed in the past decade, including numerous color figures. The individual chapters are based on the respective authors' studies and experiences, providing tips from the frontline to help researchers overcome bottlenecks in their own work. It highlights a number of cutting-edge techniques, such as 3D cell culture, microfluidic droplet technique, and microfluidic chip-mass spectrometry interfaces, offering a first-hand impression of the latest trends in the field and suggesting new research directions. Serving as both an elementary introduction and advanced guidebook, the book interests and inspires scholars and students who are currently studying microfluidics-based cell analysis methods as well as those who wish to do so.

Applications of Microfluidic Systems in Biology and Medicine

Manabu Tokeshi
Applications of Microfluidic Systems in Biology and Medicine Book PDF

Author : Manabu Tokeshi
Publisher : Springer
Page : 387 pages
File Size : 50,8 Mb
Release : 2019-04-25
Category : Science
ISBN : 9789811362293

Get Book

Applications of Microfluidic Systems in Biology and Medicine by Manabu Tokeshi Pdf

This book focuses on state-of-the-art microfluidic research in medical and biological applications. The top-level researchers in this research field explain carefully and clearly what can be done by using microfluidic devices. Beginners in the field —undergraduates, engineers, biologists, medical researchers—will easily learn to understand microfluidic-based medical and biological applications. Because a wide range of topics is summarized here, it also helps experts to learn more about fields outside their own specialties. The book covers many interesting subjects, including cell separation, protein crystallization, single-cell analysis, cell diagnosis, point-of-care testing, immunoassay, embyos/worms on a chip and organ-on-a-chip. Readers will be convinced that microfluidic devices have great potential for medical and biological applications.

Microfluidics Diagnostics

Valérie Taly,Stéphanie Descroix,Karla Perez-Toralla
Microfluidics Diagnostics Book PDF

Author : Valérie Taly,Stéphanie Descroix,Karla Perez-Toralla
Publisher : Humana
Page : 0 pages
File Size : 42,8 Mb
Release : 2024-06-04
Category : Technology & Engineering
ISBN : 1071638491

Get Book

Microfluidics Diagnostics by Valérie Taly,Stéphanie Descroix,Karla Perez-Toralla Pdf

This detailed volume explores a wide range of microfluidic-based approaches that exploit the unique features of microfluidic devices, which hold significant potential in the field of diagnosis. Beginning with a section on microchips for sample preparation, including cell-free DNA, exosomes, and cells, the book continues by covering protein marker analysis and detection, single-cell analysis, analysis of bacteria and viruses, as well as human cell-based culture and analysis. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Microfluidics Diagnostics: Methods and Protocols serves as an ideal guide for researchers investigating how microfluidics can shape the future of diagnosis.

Single-Cell Assays

Paul C.H. Li,Angela Ruohao Wu
Single Cell Assays Book PDF

Author : Paul C.H. Li,Angela Ruohao Wu
Publisher : Humana
Page : 0 pages
File Size : 51,6 Mb
Release : 2023-07-11
Category : Science
ISBN : 1071633228

Get Book

Single-Cell Assays by Paul C.H. Li,Angela Ruohao Wu Pdf

This detailed volume explores the use of single-cell assays in research for drug discovery, microfluidics, and more. The book delves into methodologies involving a variety of cell types and diseases, small molecules and biologics, as well as studies of the genome and transciptome. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Single-Cell Assays: Microfluidics, Genomics, and Drug Discovery serves to enable researchers to obtain a rapid overview in state-of-the-art microfluidic single-cell assays and an impression of what possibilities these assays offer to drug discovery.