Microfluidics In Cell Biology Part B Microfluidics In Single Cells

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Microfluidics in Cell Biology Part B: Microfluidics in Single Cells

Author : Anonim
Publisher : Academic Press
Page : 247 pages
File Size : 42,6 Mb
Release : 2018-08-27
Category : Science
ISBN : 9780128142837

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Microfluidics in Cell Biology Part B: Microfluidics in Single Cells by Anonim Pdf

Microfluidics in Cell Biology Part B: Microfluidics in Single Cells, Volume 147, a new volume in the Methods in Cell Biology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Unique to this updated volume are three sections on microfluidics in various single cell models, including microfludics in micro-organisms, microfluidics for cell culture and cell sorting of mammalian cells, and microfluidics for cell migration. Specific sections in this latest release include Temperature control and drug delivery for cell division cycle control in fission yeast H2O2 stress response in budding yeast, Antibiotic resistance in bacteria, Metabolism in bacteria, Fluidized beds for bacterial sorting and amplification, Microfluidics for cell culture and cell sorting of mammalian cells, Hydrogel microwells, Immune cells migration in complex environments, Neutrophiles migration in health and disease, Cell guidance by physical cues, Stable gradients in gels of extracellular matrix for cancer cell migration, and more. Contains contributions from experts in the field from across the world Covers a wide array of topics on both mitosis and meiosis Includes relevant, analysis based topics

Microfluidics in Cell Biology Part C: Microfluidics for Cellular and Subcellular Analysis

Author : Anonim
Publisher : Academic Press
Page : 266 pages
File Size : 53,6 Mb
Release : 2018-11-22
Category : Science
ISBN : 9780128142851

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Microfluidics in Cell Biology Part C: Microfluidics for Cellular and Subcellular Analysis by Anonim Pdf

Microfluidics in Cell Biology Part C, Volume 148, a new release in the Methods in Cell Biology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Unique to this updated volume are three sections on microfluidics in various multi-cellular models, including microfluidics in cell monolayers/spheroids, microfluidics in organ on chips, and microfluidics in model organisms. Specific chapters discuss collective migration in microtubes, leukocyte adhesion dynamics on endothelial monolayers under flow, constrained spheroid for perfusion culture, cells in droplet arrays, heart on chips, kidney on chips, liver on chips, and more. Contains contributions from experts in the field from across the world Covers a wide array of topics on both mitosis and meiosis Includes relevant, analysis based topics

Cell Analysis on Microfluidics

Author : Jin-Ming Lin
Publisher : Springer
Page : 429 pages
File Size : 41,7 Mb
Release : 2017-10-25
Category : Science
ISBN : 9789811053948

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Cell Analysis on Microfluidics by Jin-Ming Lin Pdf

This book presents a detailed overview of the design, formatting, application, and development of microfluidic chips in the context of cell biology research, enumerating each element involved in microfluidics-based cell analysis, discussing its history, status quo, and future prospects, It also offers an extensive review of the research completed in the past decade, including numerous color figures. The individual chapters are based on the respective authors' studies and experiences, providing tips from the frontline to help researchers overcome bottlenecks in their own work. It highlights a number of cutting-edge techniques, such as 3D cell culture, microfluidic droplet technique, and microfluidic chip-mass spectrometry interfaces, offering a first-hand impression of the latest trends in the field and suggesting new research directions. Serving as both an elementary introduction and advanced guidebook, the book interests and inspires scholars and students who are currently studying microfluidics-based cell analysis methods as well as those who wish to do so.

Microfluidics in Cell BiologyPart A: Microfluidics for multicellular systems

Author : Anonim
Publisher : Academic Press
Page : 273 pages
File Size : 47,9 Mb
Release : 2018-07-20
Category : Science
ISBN : 9780128142813

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Microfluidics in Cell BiologyPart A: Microfluidics for multicellular systems by Anonim Pdf

Microfluidics in Cell Biology Part A: Volume 146, the latest release in the Methods in Cell Biology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Unique to this updated volume are sections on Cell monolayers/spheroids, Collective migration in microtubes, Leukocyte adhesion dynamics on endothelial monolayers under flow, Constrained spheroid for perfusion culture, Cells in droplet arrays, Heart on chips, Kidney on chips, Liver on chips and hepatic immune responses, Gut on chips, 3D microvascular model-based lymphoma model, Blood brain barrier on chips, Multi-organ-on-a-chip for pharmacokinetic analysis, Cancer immunotherapy on chips, and more. Contains contributions from experts in the field from across the globe Covers a wide array of topics on both mitosis and meiosis Includes relevant, analysis based topics

Microfluidics for Single-Cell Analysis

Author : Jin-Ming Lin
Publisher : Springer Nature
Page : 261 pages
File Size : 54,5 Mb
Release : 2019-08-28
Category : Science
ISBN : 9789813297296

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Microfluidics for Single-Cell Analysis by Jin-Ming Lin Pdf

This book summarizes the various microfluidic-based approaches for single-cell capture, isolation, manipulation, culture and observation, lysis, and analysis. Single-cell analysis reveals the heterogeneities in morphology, functions, composition, and genetic performance of seemingly identical cells, and advances in single-cell analysis can overcome the difficulties arising due to cell heterogeneity in the diagnostics for a targeted model of disease. This book provides a detailed review of the state-of-the-art techniques presenting the pros and cons of each of these methods. It also offers lessons learned and tips from front-line investigators to help researchers overcome bottlenecks in their own studies. Highlighting a number of techniques, such as microfluidic droplet techniques, combined microfluidics-mass-spectrometry systems, and nanochannel sampling, it describes in detail a new microfluidic chip-based live single-cell extractor (LSCE) developed in the editor’s laboratory, which opens up new avenues to use open microfluidics in single-cell extraction, single-cell mass spectrometric analysis, single-cell adhesion analysis and subcellular operations. Serving as both an elementary introduction and advanced guidebook, this book interests and inspires scholars and students who are currently studying or wish to study microfluidics-based cell analysis methods.

Microfluidics for Biologists

Author : Chandra K. Dixit,Ajeet Kaushik
Publisher : Springer
Page : 252 pages
File Size : 52,7 Mb
Release : 2016-10-13
Category : Medical
ISBN : 9783319400365

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Microfluidics for Biologists by Chandra K. Dixit,Ajeet Kaushik Pdf

This book describes novel microtechnologies and integration strategies for developing a new class of assay systems to retrieve desired health information from patients in real-time. The selection and integration of sensor components and operational parameters for developing point-of-care (POC) are also described in detail. The basics that govern the microfluidic regimen and the techniques and methods currently employed for fabricating microfluidic systems and integrating biosensors are thoroughly covered. This book also describes the application of microfluidics in the field of cell and molecular biology, single cell biology, disease diagnostics, as well as the commercially available systems that have been either introduced or have the potential of being used in research and development. This is an ideal book for aiding biologists in understanding the fundamentals and applications of microfluidics. This book also: Describes the preparatory methods for developing 3-dimensional microfluidic structures and their use for Lab-on-a-Chip design Explains the significance of miniaturization and integration of sensing components to develop wearable sensors for point-of-care (POC) Demonstrates the application of microfluidics to life sciences and analytical chemistry, including disease diagnostics and separations Motivates new ideas related to novel platforms, valving technology, miniaturized transduction methods, and device integration to develop next generation sequencing Discusses future prospects and challenges of the field of microfluidics in the areas of life sciences in general and diagnostics in particular

Micropatterning in Cell Biology, Part B

Author : Anonim
Publisher : Elsevier
Page : 305 pages
File Size : 55,5 Mb
Release : 2014-01-27
Category : Technology & Engineering
ISBN : 9780124171398

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Micropatterning in Cell Biology, Part B by Anonim Pdf

This new volume of Methods in Cell Biology is the second volume describing micropatterning, complementing Volume 120. Chapters are written by experts in the field and include cutting-edge material. Includes sections on micropatterning in 2D with photomask, maskless micropatterning and 2D nanopatterning Chapters are written by experts in the field Cutting-edge material

Microfluidic Cell Culture Systems

Author : Jeffrey T Borenstein,Vishal Tandon,Sarah L Tao,Joseph L. Charest
Publisher : Elsevier
Page : 396 pages
File Size : 53,7 Mb
Release : 2018-09-12
Category : Science
ISBN : 9780128136720

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Microfluidic Cell Culture Systems by Jeffrey T Borenstein,Vishal Tandon,Sarah L Tao,Joseph L. Charest Pdf

Techniques for microfabricating intricate microfluidic structures that mimic the microenvironment of tissues and organs, combined with the development of biomaterials with carefully engineered surface properties, have enabled new paradigms in and cell culture-based models for human diseases. The dimensions of surface features and fluidic channels made accessible by these techniques are well-suited to the size scale of biological cells. Microfluidic Cell Culture Systems applies design and experimental techniques used in in microfluidics, and cell culture technologies to organ-on-chip systems. This book is intended to serve as a professional reference, providing a practical guide to design and fabrication of microfluidic systems and biomaterials for use in cell culture systems and human organ models. The book covers topics ranging from academic first principles of microfluidic design, to clinical translation strategies for cell culture protocols. The goal is to help professionals coming from an engineering background to adapt their expertise for use in cell culture and organ models applications, and likewise to help biologists to design and employ microfluidic technologies in their cell culture systems. This 2nd edition contains new material that strengthens the focus on in vitro models useful for drug discovery and development. One new chapter reviews liver organ models from an industry perspective, while others cover new technologies for scaling these models and for multi-organ systems. Other new chapters highlight the development of organ models and systems for specific applications in disease modeling and drug safety. Previous chapters have been revised to reflect the latest advances. Provides design and operation methodology for microfluidic and microfabricated materials and devices for organ-on-chip disease and safety models. This is a rapidly expanding field that will continue to grow along with advances in cell biology and microfluidics technologies. Comprehensively covers strategies and techniques ranging from academic first principles to industrial scale-up approaches. Readers will gain insight into cell-material interactions, microfluidic flow, and design principles. Offers three fundamental types of information: 1) design principles, 2) operation techniques, and 3) background information/perspectives. The book is carefully designed to strike a balance between these three areas, so it will be of use to a broad range of readers with different technical interests and educational levels.

Microtechnology for Cell Manipulation and Sorting

Author : Wonhee Lee,Peter Tseng,Dino Di Carlo
Publisher : Springer
Page : 281 pages
File Size : 51,8 Mb
Release : 2016-10-05
Category : Technology & Engineering
ISBN : 9783319441399

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Microtechnology for Cell Manipulation and Sorting by Wonhee Lee,Peter Tseng,Dino Di Carlo Pdf

This book delves into the recent developments in the microscale and microfluidic technologies that allow manipulation at the single and cell aggregate level. Expert authors review the dominant mechanisms that manipulate and sort biological structures, making this a state-of-the-art overview of conventional cell sorting techniques, the principles of microfluidics, and of microfluidic devices. All chapters highlight the benefits and drawbacks of each technique they discuss, which include magnetic, electrical, optical, acoustic, gravity/sedimentation, inertial, deformability, and aqueous two-phase systems as the dominant mechanisms utilized by microfluidic devices to handle biological samples. Each chapter explains the physics of the mechanism at work, and reviews common geometries and devices to help readers decide the type of style of device required for various applications. This book is appropriate for graduate-level biomedical engineering and analytical chemistry students, as well as engineers and scientists working in the biotechnology industry.

Microfluidics for Cells and Other Organisms

Author : Danny van Noort
Publisher : MDPI
Page : 200 pages
File Size : 44,5 Mb
Release : 2019-10-23
Category : Technology & Engineering
ISBN : 9783039215621

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Microfluidics for Cells and Other Organisms by Danny van Noort Pdf

Microfluidics-based devices play an important role in creating realistic microenvironments in which cell cultures can thrive. They can, for example, be used to monitor drug toxicity and perform medical diagnostics, and be in a static-, perfusion- or droplet-based device. They can also be used to study cell-cell, cell-matrix or cell-surface interactions. Cells can be either single cells, 3D cell cultures or co-cultures. Other organisms could include bacteria, zebra fish embryo, C. elegans, to name a few.

Microfluidics and Nanotechnology

Author : Eric Lagally
Publisher : CRC Press
Page : 290 pages
File Size : 47,6 Mb
Release : 2017-12-19
Category : Medical
ISBN : 9781466594913

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Microfluidics and Nanotechnology by Eric Lagally Pdf

An increasing number of technologies are being used to detect minute quantities of biomolecules and cells. However, it can be difficult to determine which technologies show the most promise for high-sensitivity and low-limit detection in different applications. Microfluidics and Nanotechnology: Biosensing to the Single Molecule Limit details proven approaches for the detection of single cells and even single molecules—approaches employed by the world’s foremost microfluidics and nanotechnology laboratories. While similar books concentrate only on microfluidics or nanotechnology, this book focuses on the combination of soft materials (elastomers and other polymers) with hard materials (semiconductors, metals, and glass) to form integrated detection systems for biological and chemical targets. It explores physical and chemical—as well as contact and noncontact—detection methods, using case studies to demonstrate system capabilities. Presenting a snapshot of the current state of the art, the text: Explains the theory behind different detection techniques, from mechanical resonators for detecting cell density to fiber-optic methods for detecting DNA hybridization, and beyond Examines microfluidic advances, including droplet microfluidics, digital microfluidics for manipulating droplets on the microscale, and more Highlights an array of technologies to allow for a comparison of the fundamental advantages and challenges of each, as well as an appreciation of the power of leveraging scalability and integration to achieve sensitivity at low cost Microfluidics and Nanotechnology: Biosensing to the Single Molecule Limit not only serves as a quick reference for the latest achievements in biochemical detection at the single-cell and single-molecule levels, but also provides researchers with inspiration for further innovation and expansion of the field.

Microfluidics and Multi Organs on Chip

Author : P. V. Mohanan
Publisher : Springer Nature
Page : 712 pages
File Size : 47,6 Mb
Release : 2022-07-11
Category : Medical
ISBN : 9789811913792

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Microfluidics and Multi Organs on Chip by P. V. Mohanan Pdf

This book highlights the application of microfluidics in cell biology research, chemical biology, and drug discovery. It covers the recent breakthroughs and prospects of organ-on-a-chip, human-on-a-chip, multi-organ-on-a-chip for personalized medicine. The book presents the preclinical studies of organs-on-a-chip, concepts of multiple vascularized organ-on-chips, application of organ-on-a-chip in blood-brain barrier model, culture and co-culture of cells on multi-organ-on-chip and parameter measurements in microfluidic devices. It underscores the advantage of microfluidic devices for developing efficient drug carrier particles, cell-free protein synthesis systems, and rapid techniques for direct drug screening. Further, it entails human-on-a-chip for measuring the systemic response as well as immediate effects of an organ reaction on other organs. In summary, this book reviews the development of a microfluidic-based organ-on-a-chip device for the preclinical evaluation, ADME studies of drugs, chemicals, and medical devices. This book is a valuable source for pharma companies, product developers, students, researchers, academicians, and practitioners.

Multidisciplinary Microfluidic and Nanofluidic Lab-on-a-Chip

Author : Xiujun (James) Li,Chaoyong Yang,Paul C. H. Li
Publisher : Newnes
Page : 486 pages
File Size : 42,9 Mb
Release : 2021-09-19
Category : Science
ISBN : 9780444594617

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Multidisciplinary Microfluidic and Nanofluidic Lab-on-a-Chip by Xiujun (James) Li,Chaoyong Yang,Paul C. H. Li Pdf

Multidisciplinary Microfluidic and Nanofluidic Lab-on-a-Chip: Principles and Applications provides chemists, biophysicists, engineers, life scientists, biotechnologists, and pharmaceutical scientists with the principles behind the design, manufacture, and testing of life sciences microfluidic systems. This book serves as a reference for technologies and applications in multidisciplinary areas, with an emphasis on quickly developing or new emerging areas, including digital microfluidics, nanofluidics, papers-based microfluidics, and cell biology. The book offers practical guidance on how to design, analyze, fabricate, and test microfluidic devices and systems for a wide variety of applications including separations, disease detection, cellular analysis, DNA analysis, proteomics, and drug delivery. Calculations, solved problems, data tables, and design rules are provided to help researchers understand microfluidic basic theory and principles and apply this knowledge to their own unique designs. Recent advances in microfluidics and microsystems for life sciences are impacting chemistry, biophysics, molecular, cell biology, and medicine for applications that include DNA analysis, drug discovery, disease research, and biofluid and environmental monitoring. Provides calculations, solved problems, data tables and design rules to help understand microfluidic basic theory and principles Gives an applied understanding of the principles behind the design, manufacture, and testing of microfluidic systems Emphasizes on quickly developing and emerging areas, including digital microfluidics, nanofluidics, papers-based microfluidics, and cell biology

Micro/Nanofluidics and Lab-on-Chip Based Emerging Technologies for Biomedical and Translational Research Applications - Part B

Author : Anonim
Publisher : Academic Press
Page : 370 pages
File Size : 45,6 Mb
Release : 2022-01-28
Category : Science
ISBN : 9780323853040

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Micro/Nanofluidics and Lab-on-Chip Based Emerging Technologies for Biomedical and Translational Research Applications - Part B by Anonim Pdf

Micro/Nanofluidics and Lab-on-Chip Based Emerging Technologies for Biomedical and Translational Research Applications - Part B, Volume 187 represents the collation of chapters written by eminent scientists worldwide. Chapters in this new release include Design and fabrication of microfluidics devices for molecular biology applications, Micro/Nanofluidics devices for drug delivery, From organ-on-chip to body-on-chip: the next generation of microfluidics platforms for in vitro drug toxicity testing, Micro/Nanofluidics for high throughput drug screening, Design, fabrication and assembly of lab-on-a-chip and its uses, Advances in microfluidic 3D cell culture for pre-clinical drug development, Tissue and organ culture on lab-on-a chip for biomedical applications, and much more. Offers a basic understanding of the state-of-the-art design and fabrication of microfluidics/ nanofluidics and lab on chip Explains how to develop microfluidics/nanofluidic for advanced application such as healthcare, high throughout drug screening, 3D cell culture and organ-on-chip Discusses the emerging demands and research of micro/nanofluidic based devices in biomedical and translational research applications

Nanotechnology for Microfluidics

Author : Xingyu Jiang
Publisher : John Wiley & Sons
Page : 444 pages
File Size : 45,7 Mb
Release : 2020-09-08
Category : Technology & Engineering
ISBN : 9783527345335

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Nanotechnology for Microfluidics by Xingyu Jiang Pdf

The book focuses on microfluidics with applications in nanotechnology. The first part summarizes the recent advances and achievements in the field of microfluidic technology, with emphasize on the the influence of nanotechnology. The second part introduces various applications of microfluidics in nanotechnology, such as drug delivery, tissue engineering and biomedical diagnosis.