Molecular Methods In Ecology

Author : Allan Baker
Publisher : John Wiley & Sons
Page : 349 pages
File Size : 49,9 Mb
Release : 2009-04-01
Category : Science
ISBN : 9781444311419

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Molecular Methods in Ecology by Allan Baker Pdf

The incorporation of molecular methods in ecological research has added an exciting new dimension to conventional studies, and opened windows into previously intractable areas of research, at the interface between ecology and genetics. Using these new methods it has now become routine to use genetic markers to study ecological phenomena, from molecular sexing of individuals and parentage of offspring, through to population structure of species and phylogenetic relationships of taxa. These methods have stimulated an explosion of empirical and analytical developments in molecular ecology, which have in turn, increasingly attracted students and professional biologists eager to employ them in their studies. Molecular Methods in Ecology traces the development of molecular ecology by reviewing basic molecular biological techniques and earlier methods such as protein electrophoresis, DNA-DNA hybridisation, restriction analysis of DNA, and DNA fingerprinting. Later chapters review methods using newer classes of markers such as microsatellites, introns, MHC, SSRs and AFLP markers in plants and molecular sexing in animals. The strengths and limitations of methods are discussed and guidance is provided in selecting the most appropriate methods for particular problems in ecology. This book will provide both postgraduates and researchers with a guide to choosing and employing appropriate methodologies for successful research in the field of molecular ecology. Provides up-to-date summaries of the latest molecular approaches in this rapidly expanding field. Gives guidance on the appropriate choice of methods for particular problems in ecology, and their strengths and limitations. Provides brief laboratory protocols for each molecular method and summaries of software available for analysis of data in molecular ecology. Outlines examples of the latest research results from studies of both plants and animals, integrated within the framework of molecular ecology.

Author : Mark Osborn,Cindy Smith
Publisher : Taylor & Francis
Page : 256 pages
File Size : 48,7 Mb
Release : 2004-06-02
Category : Science
ISBN : 9781135320416

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Molecular Microbial Ecology by Mark Osborn,Cindy Smith Pdf

Microoganisms are distributed across every ecosystem, and microbial transformations are fundamental to the operation of the biosphere. Microbial ecology is the study of this interaction between microorganisms and their environment, and arguably represents one of the most important areas of biological research. Yet for many years our study of microbial flora was severely limited: the primary method of culturing microorganisms on media allowed us to study only between 0.1 and 10% of the total microbial flora in any given environment. Molecular Microbial Ecology gives a comprehensive guide to the recent revolution in the study of microorganisms in the environment. Details are given on molecular methods for isolating some of the previously uncultured and numerically dominant microbial groups. PCR-based approaches to studying prokaryotic systematics are described, including ribosomal RNA analysis and stable isotope probing. Later chapters cover DNA hybridisation techniques (including fluorescent in situ hybridisation), as well as genomic and metagenomic approaches to microbial ecology. Gathering together some of the world’s leading experts, this book provides an invaluable introduction to the modern theory and molecular methods used in studying microbial ecology.

Author : R. deSalle,Bernd Schierwater
Publisher : Springer Science & Business Media
Page : 390 pages
File Size : 49,8 Mb
Release : 1998-09-29
Category : Medical
ISBN : 3764357258

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Molecular Approaches To Ecology And Evolution by R. deSalle,Bernd Schierwater Pdf

"The last ten years have seen an explosion of activity in the application of molecular biological techniques to evolutionary and ecological studies. This volume attempts to summarize advances in the field and place into context the wide variety of methods available to ecologists and evolutionary biologists using molecular techniques. Both the molecular techniques and the variety of methods available for the analysis of such data are presented in the text. The book has three major sections - populations, species and higher taxa. Each of these sections contains chapters by leading scientists working at these levels, where clear and concise discussion of technology and implication of results are presented. The volume is intended for advanced students of ecology and evolution and would be a suitable textbook for advanced undergraduate and graduate student seminar courses." -- Publisher.

Author : B. Schierwater,B. Streit,G.P. Wagner,R. DeSalle
Publisher : Birkhäuser
Page : 626 pages
File Size : 41,9 Mb
Release : 2013-06-29
Category : Science
ISBN : 9783034875271

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Molecular Ecology and Evolution: Approaches and Applications by B. Schierwater,B. Streit,G.P. Wagner,R. DeSalle Pdf

The past 25 years have witnessed a revolution in the way ecologists and evolutionary biologists approach their disciplines. Modern molecular techniques are now reshaping the spectrum of questions that can be addressed while studying the mechanisms and consequences of the ecology and evolution of living organisms. "Molecular Ecology and Evolution: Approaches and Applications" describes, from a molecular perspective, several methodological and technical approaches used in the fields of ecology, evolution, population biology, molecular systematics, conservation genetics, and development. Modern techniques are introduced, and older, more classic ones refined. The advantages, limitations, and potentials of each are discussed in detail, and thereby illustrate the widening range of cross-field research and applications which this modern technology is stimulating. This book will serve as an important textbook for graduate and advanced undergraduate students, and as a key reference work for researchers

Author : Antoon D. L. Akkermans,Jan Dirk Van Elsas,Frans J. De Bruijn
Publisher : Springer
Page : 266 pages
File Size : 40,7 Mb
Release : 2014-01-13
Category : Science
ISBN : 9789400902152

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Molecular Microbial Ecology Manual by Antoon D. L. Akkermans,Jan Dirk Van Elsas,Frans J. De Bruijn Pdf

For a long time microbial ecology has been developed as a distinct field within Ecology. In spite of the important role of microorganisms in the environment, this group of 'invisible' organisms remained unaccessable to other ecologists. Detection and identification of microorganisms remain largely dependent on isolation techniques and characterisation of pure cul tures. We now realise that only a minor fraction of the microbial com munity can be cultivated. As a result of the introduction of molecular methods, microbes can now be detected and identified at the DNA/RNA level in their natural environment. This has opened a new field in ecology: Molecular Microbial Ecology. In the present manual we aim to introduce the microbial ecologist to a selected number of current molecular techniques that are relevant in micro bial ecology. The first edition of the manual contains 33 chapters and an equal number of additional chapters will be added this year. Since the field of molecular ecology is in a continuous progress, we aim to update and extend the Manual regularly and will invite anyone to depo sit their new protocols in full detail in the next edition of this Manual. We hope this book finds its place where it was born: at the lab bench! Antoon D.L. Akkermans, Jan Dirk van Elsas and Frans J. de Bruijn March 1995 Molecular Microbial Ecology Manual 1.3.6: 1-8, 1996. © 1996 Kluwer Academic Publishers.

Author : Trevor Beebee,Graham Rowe
Publisher : Oxford University Press
Page : 418 pages
File Size : 50,8 Mb
Release : 2008
Category : Juvenile Nonfiction
ISBN : 9780199292059

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An Introduction to Molecular Ecology by Trevor Beebee,Graham Rowe Pdf

How do we know whether a particular species is monogamous or promiscuous? How can we monitor the illegal trafficking of wildlife? How can we differentiate between the many similar species making up a microbial community? An Introduction to Molecular Ecology introduces the latest molecular concepts and techniques, demonstrating how genetic markers and molecular tools can be used to answer such ecological questions. Such questions, whose answers were previously out of our reach, can now be probed, thereby revolutionizing our understanding of ecological systems and phenomena. Blending conceptual detail with the most instructive examples, An Introduction to Molecular Ecology is an ideal resource for those new to the subject needing to develop a strong working understanding of the field. The book captures the broad scope of the subject, exploring the use of molecular tools in the context of topics including behavioral genetics, phylogeography, microbial ecology, and conservation. Features - Demonstrates the power of molecular ecology as a research tool in a style ideally suited for an undergraduate audience - Uses practical examples to demonstrate the latest methods and concepts rather than relying exclusively on theoretical models - Blends factual content with tools for active learning

Author : Luca Cocolin,Danilo Ercolini
Publisher : Springer Science & Business Media
Page : 291 pages
File Size : 47,9 Mb
Release : 2007-12-15
Category : Technology & Engineering
ISBN : 9780387745206

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Molecular Techniques in the Microbial Ecology of Fermented Foods by Luca Cocolin,Danilo Ercolini Pdf

With the application of new analytical techniques, the field of food fermentation has grown in recent years. This book provides the latest information and relevant advances on the microbial ecology of fermented foods and the application of molecular methods. This book serves as a guide for students and researchers on the most advanced techniques to identify bacteria and helps in choosing the most appropriate tools to study fermented food from a microbiological point of view.

Author : Joanna R. Freeland
Publisher : John Wiley & Sons
Page : 403 pages
File Size : 53,9 Mb
Release : 2006-03-30
Category : Science
ISBN : 9780470090633

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Molecular Ecology by Joanna R. Freeland Pdf

Molecular Ecology provides a comprehensive introduction to the many diverse aspects of this subject. The book unites theory with examples from a wide range of taxa in a logical and progressive manner, and its accessible writing style makes subjects such as population genetics and phylogenetics highly comprehensible to its readers. The first part of the book introduces the essential underpinnings of molecular ecology, starting with a review of genetics and a discussion of the molecular markers that are most frequently used in ecological research. This leads into an overview of population genetics in ecology. The second half of the book then moves on to specific applications of molecular ecology, covering phylogeography, behavioural ecology and conservation genetics. The final chapter looks at molecular ecology in a wider context by using a number of case studies that are relevant to various economic and social concerns, including wildlife forensics, agriculture, and overfishing * comprehensive overview of the different aspects of molecular ecology * attention to both theoretical and applied concerns * accessible writing style and logical structure * numerous up-to-date examples and references This will be an invaluable reference for those studying molecular ecology, population genetics, evolutionary biology, conservation genetics and behavioural ecology, as well as researchers working in these fields.

Author : Rob DeSalle,Gonzalo Giribet,Ward Wheeler
Publisher : Birkhäuser
Page : 414 pages
File Size : 46,9 Mb
Release : 2013-12-01
Category : Science
ISBN : 9783034881258

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Techniques in Molecular Systematics and Evolution by Rob DeSalle,Gonzalo Giribet,Ward Wheeler Pdf

The amount of information that can be obtained by using molecular techniques in evolution, systematics and ecology has increased exponentially over the last ten years. The need for more rapid and efficient methods of data acquisition and analysis is growing accordingly. This manual presents some of the most important techniques for data acquisition developed over the last years. The choice and justification of data analysis techniques is also an important and critical aspect of modern phylogenetic and evolutionary analysis and so a considerable part of this volume addresses this important subject. The book is mainly written for students and researchers from evolutionary biology in search for methods to acquire data, but also from molecular biology who might be looking for information on how data are analyzed in an evolutionary context. To aid the user, information on web-located sites is included wherever possible. Approaches that will push the amount of information which systematics will gather in the

Author : Andrew Lowe,Stephen Harris,Paul Ashton
Publisher : John Wiley & Sons
Page : 432 pages
File Size : 52,8 Mb
Release : 2009-04-01
Category : Science
ISBN : 9781444311211

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Ecological Genetics by Andrew Lowe,Stephen Harris,Paul Ashton Pdf

Ecological Genetics addresses the fundamental problems of which of the many molecular markers should be used and how the resulting data should be analysed in clear, accessible language, suitable for upper-level undergraduates through to research-level professionals. A very accessible straightforward text to deal with this difficult topic - applying modern molecular techniques to ecological processes. Written by active researchers and teachers within the field. There will be an accompanying web site managed by the authors, comprising of worked examples, test data sets and hyperlinks to relevant web pages.

Author : Akke Jitske Van Der Zijpp,Jan Dirk Van Elsas,F. J. de Bruijn
Publisher : Unknown
Page : 514 pages
File Size : 42,5 Mb
Release : 2014-01-15
Category : Electronic
ISBN : 9401103526

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Molecular Microbial Ecology Manual by Akke Jitske Van Der Zijpp,Jan Dirk Van Elsas,F. J. de Bruijn Pdf

Author : A. Mark Osborn,Cindy Jane Smith
Publisher : Garland Science
Page : 404 pages
File Size : 41,8 Mb
Release : 2005
Category : Science
ISBN : 1859962831

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Molecular Microbial Ecology by A. Mark Osborn,Cindy Jane Smith Pdf

Molecular approaches to microbial ecology mean that in principle the entire range of species in a given sample can be analysed, in contrast to conventional techniques which rely on cell cultivation, and thereby typically include only about 10% of the species in a sample. Molecular Microbial Ecology provides both a theoretical and a methodological introduction to various key molecular techniques used in microbial ecology, focusing particularly on nucleic acid-based methodologies.

Author : D. Renouf
Publisher : Springer Science & Business Media
Page : 424 pages
File Size : 47,6 Mb
Release : 2012-12-06
Category : Science
ISBN : 9789401131001

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The Behaviour of Pinnipeds by D. Renouf Pdf

We teach our students of behavioural science that one first defines a research problem, and then the most appropriate animal is selected to investigate hypotheses. The reverse order of events is improper: a particular class of animals should not be studied for its own sake. In the case of the Pinnipeds (seals, sea lions, fur seals and walruses) the organism and the problem are essentially the same. The research questions presented in this volume in one way or another relate to survival in two worlds, the ocean for foraging, and the terrain at its edge or frozen above it for breeding. The evolution of Pinniped behaviour and the mechanisms which underlie it are a consequence of having to cope with two seemingly incompatible sets of environmental constraints. The physiological adaptations for concomitant functioning in two media with very different physical characteristics have produced correlated behav ioural modifications. The energetic demands of reproduction and foraging are idiosyncratic because each activity occurs on opposite sides of the air/water interface. As a result, the mating system must reconcile aquatic design for such functions as locomotion and thermoregulation, with the terrestrial requirements for successful pupping. Similarly, the ecology of this dual habitat prescribes the rules governing the behaviour of the neonate and its interactions with its mother.

Author : Ian Joint
Publisher : Springer Science & Business Media
Page : 422 pages
File Size : 50,5 Mb
Release : 2013-06-29
Category : Science
ISBN : 9783642799235

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Molecular Ecology of Aquatic Microbes by Ian Joint Pdf

A NATO ASI on "Molecular Ecology of Aquatic Microbes" was held at II Ciocco, Lucca, Italy from 28 August - 9 September 1994. The aims of the ASI were to evaluate the potential for molecular biology to solve some important questions in aquatic microbiology, particularly in relation to biogeochemical cycling and microbial physiology. Techniques developed by molecular biologists have now been adopted by a wide range of scientific disciplines. In the last 5 years, aquatic microbial ecologists have begun to incorporate these methods into their research and, as a result, are developing a much clearer understanding of phylogenetic diversity, the molecular basis of physiological acclimations and the transduction of environmental signals and organism responses. The aim of this ASI was to assess progress in this new field of research, to compare and describe techniques and experimental approaches, and to foster communication between disciplines. The ASI offered an excellent opportunity to bring together aquatic ecologists with molecular biologists and to encourage efficient technology transfer. The meeting of information on the status provided a forum for detailed and broad exchange and trends of aquatic molecular ecology and to assess how emerging molecular techniques might solve some important problems in ecology which have prove intractable because of lack of appropriate methodologies.

Author : Virginie Orgogozo,Matthew V. Rockman
Publisher : Humana Press
Page : 503 pages
File Size : 42,6 Mb
Release : 2011-03-15
Category : Medical
ISBN : 1617792276

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Molecular Methods for Evolutionary Genetics by Virginie Orgogozo,Matthew V. Rockman Pdf

We are entering a particularly fruitful period in evolutionary genetics, as rapid technological progress transforms the investigation of genetic variation within and between species. Molecular Methods for Evolutionary Genetics is a collection of advanced molecular biology protocols and general overviews intended to represent the essential methods currently bringing evolutionary genetics to fruition. Divided into six thematic sections, this volume covers methods for characterizing genomes, diverse approaches to enrich DNA for subsets of the genome prior to sequencing, and state-of-the-art protocols for sampling genetic variation for genetic mapping studies and population genetic studies (RAD sequencing, Sequenom, microarrays, etc.). The volume concludes by focusing on methods to study candidate genes, from obtaining their sequences and analyzing their transcripts to experimentally manipulating their activities in vivo. Written in the highly successful Methods in Molecular BiologyTM series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Molecular Methods for Evolutionary Genetics serves as a rich resource to biologists interested in evolution, whether they be specialists or beginners in molecular biology.