Molecular Microbial Ecology Manual

Author : Antoon D. L. Akkermans,Jan Dirk Van Elsas,Frans J. De Bruijn
Publisher : Springer
Page : 266 pages
File Size : 42,7 Mb
Release : 2014-01-13
Category : Science
ISBN : 9789400902152

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Molecular Microbial Ecology Manual by Antoon D. L. Akkermans,Jan Dirk Van Elsas,Frans J. De Bruijn Pdf

For a long time microbial ecology has been developed as a distinct field within Ecology. In spite of the important role of microorganisms in the environment, this group of 'invisible' organisms remained unaccessable to other ecologists. Detection and identification of microorganisms remain largely dependent on isolation techniques and characterisation of pure cul tures. We now realise that only a minor fraction of the microbial com munity can be cultivated. As a result of the introduction of molecular methods, microbes can now be detected and identified at the DNA/RNA level in their natural environment. This has opened a new field in ecology: Molecular Microbial Ecology. In the present manual we aim to introduce the microbial ecologist to a selected number of current molecular techniques that are relevant in micro bial ecology. The first edition of the manual contains 33 chapters and an equal number of additional chapters will be added this year. Since the field of molecular ecology is in a continuous progress, we aim to update and extend the Manual regularly and will invite anyone to depo sit their new protocols in full detail in the next edition of this Manual. We hope this book finds its place where it was born: at the lab bench! Antoon D.L. Akkermans, Jan Dirk van Elsas and Frans J. de Bruijn March 1995 Molecular Microbial Ecology Manual 1.3.6: 1-8, 1996. © 1996 Kluwer Academic Publishers.

Author : George A. Kowalchuk
Publisher : Springer Science & Business Media
Page : 1760 pages
File Size : 49,9 Mb
Release : 2004-08-09
Category : Nature
ISBN : 9781402021763

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Molecular Microbial Ecology Manual by George A. Kowalchuk Pdf

Microbes are key drivers of the world's ecosystems. The vast majority of the world's diversity and metabolic potential lies within micro-organisms, yet we are just beginning to understand and utilize this ultimate resource of biological diversity. Critical to our exploration of the microbial world are methods that allow for the analysis of organisms that are invisible to our eyes, difficult to distinguish from each other, and often impossible to grow using available culture methods. The field of microbial ecology has been revolutionized in the past two decades by the introduction of molecular methods into the toolbox of the microbial ecologist. This molecular arsenal has helped to unveil the enormity of microbial diversity across the breadth of the earth's ecosystems, and has revealed that we are only familiar with a very small minority of the organisms that carry out key microbial functions in diverse habitats. The Molecular Microbial Ecology Manual, Second Edition (MMEM-II) provides a detailed and user-friendly description of the methods that have made this revolution in microbial ecology possible. However, what is perhaps most exciting about MMEM-II is that it contains a large number of new chapters, highlighting the newest trends in microbial ecology research, which seek to provide more quantitative and statistically robust data, and means of coupling microbial identity and function. In addition, the majority of the proven methods described in MMEM's first version have undergone significant revisions to provide the most up-to-date applications available. The state-of-the-art methods described in MMEM-II have not only been provided by experts in the field, but in most cases by the laboratories that actually first developed and applied the methods, thus providing the MMEM-II user with unique first-hand tips and insight. The new on-line format available forMMEM-II should also add to the utility of MMEM-II by allowing users to search for key topics throughout the manual, skip between interrelated chapters at the push of a button, and by providing immediate availability to protocol updates and new chapters dedicated to future technical developments.

Author : Anonim
Publisher : Unknown
Page : 849 pages
File Size : 52,8 Mb
Release : 2004
Category : Electronic
ISBN : OCLC:835573405

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Molecular Microbial Ecology Manual by Anonim Pdf

Author : Frans J. de Bruijn
Publisher : John Wiley & Sons
Page : 1155 pages
File Size : 49,6 Mb
Release : 2011-10-14
Category : Science
ISBN : 9781118010495

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Handbook of Molecular Microbial Ecology I by Frans J. de Bruijn Pdf

The premiere two-volume reference on revelations from studying complex microbial communities in many distinct habitats Metagenomics is an emerging field that has changed the way microbiologists study microorganisms. It involves the genomic analysis of microorganisms by extraction and cloning of DNA from a group of microorganisms, or the direct use of the purified DNA or RNA for sequencing, which allows scientists to bypass the usual protocol of isolating and culturing individual microbial species. This method is now used in laboratories across the globe to study microorganism diversity and for isolating novel medical and industrial compounds. Handbook of Molecular Microbial Ecology is the first comprehensive two-volume reference to cover unculturable microorganisms in a large variety of habitats, which could not previously have been analyzed without metagenomic methodology. It features review articles as well as a large number of case studies, based largely on original publications and written by international experts. This first volume, Metagenomics and Complementary Approaches, covers such topics as: Background information on DNA reassociation and use of 16 rRNA and other DNA fingerprinting approaches Species designation in microbiology Metagenomics: Introduction to the basic tools with examples Consortia and databases Bioinformatics Computer-assisted analysis Complementary approaches—microarrays, metatranscriptomics, metaproteomics, metabolomics, and single cell analysis A special feature of this volume is the highlighting of the databases and computer programs used in each study; they are listed along with their sites in order to facilitate the computer-assisted analysis of the vast amount of data generated by metagenomic studies. Handbook of Molecular Microbial Ecology I is an invaluable reference for researchers in metagenomics, microbiology, and environmental microbiology; those working on the Human Microbiome Project; microbial geneticists; molecular microbial ecologists; and professionals in molecular microbiology and bioinformatics.

Author : Frans J. de Bruijn
Publisher : John Wiley & Sons
Page : 632 pages
File Size : 50,9 Mb
Release : 2011-10-14
Category : Science
ISBN : 9781118010532

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Handbook of Molecular Microbial Ecology II by Frans J. de Bruijn Pdf

The premiere two-volume reference on revelations from studying complex microbial communities in many distinct habitats Metagenomics is an emerging field that has changed the way microbiologists study microorganisms. It involves the genomic analysis of microorganisms by extraction and cloning of DNA from a group of microorganisms, or the direct use of the purified DNA or RNA for sequencing, which allows scientists to bypass the usual protocol of isolating and culturing individual microbial species. This method is now used in laboratories across the globe to study microorganism diversity and for isolating novel medical and industrial compounds. Handbook of Molecular Microbial Ecology is the first comprehensive two-volume reference to cover unculturable microorganisms in a large variety of habitats, which could not previously have been analyzed without metagenomic methodology. It features review articles as well as a large number of case studies, based largely on original publications and written by international experts. This second volume, Metagenomics in Different Habitats, covers such topics as: Viral genomes Metagenomics studies in a variety of habitats, including marine environments and lakes, soil, and human and animal digestive tracts Other habitats, including those involving microbiome diversity in human saliva and functional intestinal metagenomics; diversity of archaea in terrestrial hot springs; and microbial communities living at the surface of building stones Biodegradation Biocatalysts and natural products A special feature of this book is the highlighting of the databases and computer programs used in each study; they are listed along with their sites in order to facilitate the computer-assisted analysis of the vast amount of data generated by metagenomic studies. Such studies in a variety of habitats are described here, which present a large number of different system-dependent approaches in greatly differing habitats. Handbook of Molecular Microbial Ecology II is an invaluable reference for researchers in metagenomics, microbial ecology, microbiology, and environmental microbiology; those working on the Human Microbiome Project; microbial geneticists; and professionals in molecular microbiology and bioinformatics.

Author : Antoon D. L. Akkermans,Frans J. de Bruijn,Jan Dirk van Elsas
Publisher : Unknown
Page : 0 pages
File Size : 46,5 Mb
Release : 1996
Category : Electronic
ISBN : OCLC:1431293981

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Molecular Microbial Ecology Manual by Antoon D. L. Akkermans,Frans J. de Bruijn,Jan Dirk van Elsas Pdf

Author : Anonim
Publisher : Unknown
Page : 0 pages
File Size : 49,8 Mb
Release : 2004
Category : Ecology
ISBN : OCLC:1184137786

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Molecular Microbial Ecology Manual: folletos: Contents by Anonim Pdf

Section 1: Isolation of nucleic acids. Section 2: Detection of microbial nucleic acid sequences. Section 3: Identification and classification of microbes using DNA and RNA sequences. Section 4: Detection, identification and classification of microbes using other methods. Section 5: Detection of gene transfer in the environment. Section 6: Tracking of specific microbes in the environment. Section 7: Statistical, computer-assisted and other analyses. Section 8: Molecular tools to assess microbial activities.

Author : Mark Osborn,Cindy Smith
Publisher : Garland Science
Page : 370 pages
File Size : 51,5 Mb
Release : 2004-03-01
Category : Science
ISBN : 9780203503393

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Molecular Microbial Ecology by Mark Osborn,Cindy Smith Pdf

Microoganisms are distributed across every ecosystem, and microbial transformations are fundamental to the operation of the biosphere. Microbial ecology is the study of this interaction between microorganisms and their environment, and arguably represents one of the most important areas of biological research. Yet for many years our study of microbial flora was severely limited: the primary method of culturing microorganisms on media allowed us to study only between 0.1 and 10% of the total microbial flora in any given environment. Molecular Microbial Ecology gives a comprehensive guide to the recent revolution in the study of microorganisms in the environment. Details are given on molecular methods for isolating some of the previously uncultured and numerically dominant microbial groups. PCR-based approaches to studying prokaryotic systematics are described, including ribosomal RNA analysis and stable isotope probing. Later chapters cover DNA hybridisation techniques (including fluorescent in situ hybridisation), as well as genomic and metagenomic approaches to microbial ecology. Gathering together some of the world’s leading experts, this book provides an invaluable introduction to the modern theory and molecular methods used in studying microbial ecology.

Author : Frans J. de Bruijn
Publisher : John Wiley & Sons
Page : 632 pages
File Size : 46,9 Mb
Release : 2011-09-27
Category : Science
ISBN : 9780470647196

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Handbook of Molecular Microbial Ecology II by Frans J. de Bruijn Pdf

The premiere two-volume reference on revelations from studying complex microbial communities in many distinct habitats Metagenomics is an emerging field that has changed the way microbiologists study microorganisms. It involves the genomic analysis of microorganisms by extraction and cloning of DNA from a group of microorganisms, or the direct use of the purified DNA or RNA for sequencing, which allows scientists to bypass the usual protocol of isolating and culturing individual microbial species. This method is now used in laboratories across the globe to study microorganism diversity and for isolating novel medical and industrial compounds. Handbook of Molecular Microbial Ecology is the first comprehensive two-volume reference to cover unculturable microorganisms in a large variety of habitats, which could not previously have been analyzed without metagenomic methodology. It features review articles as well as a large number of case studies, based largely on original publications and written by international experts. This second volume, Metagenomics in Different Habitats, covers such topics as: Viral genomes Metagenomics studies in a variety of habitats, including marine environments and lakes, soil, and human and animal digestive tracts Other habitats, including those involving microbiome diversity in human saliva and functional intestinal metagenomics; diversity of archaea in terrestrial hot springs; and microbial communities living at the surface of building stones Biodegradation Biocatalysts and natural products A special feature of this book is the highlighting of the databases and computer programs used in each study; they are listed along with their sites in order to facilitate the computer-assisted analysis of the vast amount of data generated by metagenomic studies. Such studies in a variety of habitats are described here, which present a large number of different system-dependent approaches in greatly differing habitats. Handbook of Molecular Microbial Ecology II is an invaluable reference for researchers in metagenomics, microbial ecology, microbiology, and environmental microbiology; those working on the Human Microbiome Project; microbial geneticists; and professionals in molecular microbiology and bioinformatics.

Author : A. Mark Osborn,Cindy Jane Smith
Publisher : Garland Science
Page : 404 pages
File Size : 49,7 Mb
Release : 2005
Category : Science
ISBN : 1859962831

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Molecular Microbial Ecology by A. Mark Osborn,Cindy Jane Smith Pdf

Molecular approaches to microbial ecology mean that in principle the entire range of species in a given sample can be analysed, in contrast to conventional techniques which rely on cell cultivation, and thereby typically include only about 10% of the species in a sample. Molecular Microbial Ecology provides both a theoretical and a methodological introduction to various key molecular techniques used in microbial ecology, focusing particularly on nucleic acid-based methodologies.

Author : Harinder P.S. Makkar,Christopher S. McSweeney
Publisher : Springer Science & Business Media
Page : 221 pages
File Size : 52,5 Mb
Release : 2006-02-23
Category : Science
ISBN : 9781402037917

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Methods in Gut Microbial Ecology for Ruminants by Harinder P.S. Makkar,Christopher S. McSweeney Pdf

Asaresultofvarioushumanactivities,suchasincreaseinhumanpopulation,decrease in arable land due to soil degradation, urbanization, industrialization and associated increase in the demand for livestock products, dramatic changes are occurring in the global ruminant livestock sector. These changes includeshift inthesize of regional livestock populations and in the types of management and feeding systems under which ruminant livestock are held, and increased demand of a wider range of quality attributes from animal agriculture, not just of the products themselves but also of the methods used in their production. The livestock sector will need to respond to newchallengesofincreasinglivestockproductivitywhileprotectingenvironmentand human health and conservingbiodiversity and natural resources. The micro-organisms in the digestive tracts of ruminant livestock have a profound in?uence on the conversion offeedinto end products, which can impact on the- imal and theenvironment. As the livestock sector grows particularly in developing countries, there will be an increasing need to understand these processes for b- ter management and use ofbothfeed and other natural resources that underpinthe development of sustainable feeding systems.

Author : Cindy H. Nakatsu,Robert V. Miller,Suresh D. Pillai
Publisher : John Wiley & Sons
Page : 128 pages
File Size : 40,6 Mb
Release : 2020-08-11
Category : Science
ISBN : 9781555818821

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Manual of Environmental Microbiology by Cindy H. Nakatsu,Robert V. Miller,Suresh D. Pillai Pdf

The single most comprehensive resource for environmental microbiology Environmental microbiology, the study of the roles that microbes play in all planetary environments, is one of the most important areas of scientific research. The Manual of Environmental Microbiology, Fourth Edition, provides comprehensive coverage of this critical and growing field. Thoroughly updated and revised, the Manual is the definitive reference for information on microbes in air, water, and soil and their impact on human health and welfare. Written in accessible, clear prose, the manual covers four broad areas: general methodologies, environmental public health microbiology, microbial ecology, and biodegradation and biotransformation. This wealth of information is divided into 18 sections each containing chapters written by acknowledged topical experts from the international community. Specifically, this new edition of the Manual Contains completely new sections covering microbial risk assessment, quality control, and microbial source tracking Incorporates a summary of the latest methodologies used to study microorganisms in various environments Synthesizes the latest information on the assessment of microbial presence and microbial activity in natural and artificial environments The Manual of Environmental Microbiology is an essential reference for environmental microbiologists, microbial ecologists, and environmental engineers, as well as those interested in human diseases, water and wastewater treatment, and biotechnology.

Author : Gudni G. Hardarson,William J. Broughton
Publisher : Springer Science & Business Media
Page : 180 pages
File Size : 41,7 Mb
Release : 2013-03-09
Category : Nature
ISBN : 9789401723213

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Molecular Microbial Ecology of the Soil by Gudni G. Hardarson,William J. Broughton Pdf

Grain legume crops, e.g. common bean (Phaseolus vulgaris L.), and soyabeans (Glycine max L.) are amongst the main sources of protein in Africa, Asia and Latin America. Their high protein content derive from their ability, in symbiosis with Rhizobium bacteria, to fix atmospheric nitrogen. Incorporating contributions from molecular biologists, microbiologists, plant breeders and soil scientists, this volume reports the results of an FAO/IAEA Co-ordinated Research Programme (1992-1996), whose main objective was to develop molecular biological methods to study rhizobial ecology. Use of better tracking methods will help enhance biological nitrogen fixation and thus grain legume yields, while reducing their reliance on soil- and/or fertilizer-nitrogen. This volume will be invaluable to scientists working on biological nitrogen fixation, soil microbial ecology and legume production.

Author : A. D. L. Akkermans,Frans J. de Bruijn,Jan D. Elsas
Publisher : Springer
Page : 534 pages
File Size : 46,7 Mb
Release : 1995-06
Category : Juvenile Nonfiction
ISBN : UVA:X002680448

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AKKERMANS MOLECULAR MICRO ECOL, by A. D. L. Akkermans,Frans J. de Bruijn,Jan D. Elsas Pdf

This text offers general information about molecular techniques relevant to molecular ecological research. Attention is paid to the use of molecular methods for studying the fate of genetically modified and native microorganisms in the environment.

Author : Paul F. Kemp,Jonathan J. Cole,Barry F. Sherr,Evelyn B. Sherr
Publisher : Routledge
Page : 823 pages
File Size : 53,9 Mb
Release : 2018-05-02
Category : Science
ISBN : 9781351442367

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Handbook of Methods in Aquatic Microbial Ecology by Paul F. Kemp,Jonathan J. Cole,Barry F. Sherr,Evelyn B. Sherr Pdf

Handbook of Methods in Aquatic Microbial Ecology is the first comprehensive compilation of 85 fundamental methods in modern aquatic microbial ecology. Each method is presented in a detailed, step-by-step format that allows readers to adopt new methods with little difficulty. The methods represent the state of the art, and many have become standard procedures in microbial research and environmental assessment. The book also presents practical advice on how to apply the methods. It will be an indispensable reference for marine and freshwater research laboratories, environmental assessment laboratories, and industrial research labs concerned with microbial measurements in water.