Molecular Microbiology

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Molecular Microbiology

David H. Persing,Fred C. Tenover,Randall T. Hayden,Margareta Ieven,Melissa B. Miller,Frederick S. Nolte,Yi-Wei Tang,Alex van Belkum
Molecular Microbiology Book PDF

Author : David H. Persing,Fred C. Tenover,Randall T. Hayden,Margareta Ieven,Melissa B. Miller,Frederick S. Nolte,Yi-Wei Tang,Alex van Belkum
Publisher : John Wiley & Sons
Page : 835 pages
File Size : 48,9 Mb
Release : 2020-07-24
Category : Science
ISBN : 9781555819071

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Molecular Microbiology by David H. Persing,Fred C. Tenover,Randall T. Hayden,Margareta Ieven,Melissa B. Miller,Frederick S. Nolte,Yi-Wei Tang,Alex van Belkum Pdf

Presenting the latest molecular diagnostic techniques in one comprehensive volume The molecular diagnostics landscape has changed dramatically since the last edition of Molecular Microbiology: Diagnostic Principles and Practice in 2011. With the spread of molecular testing and the development of new technologies and their opportunities, laboratory professionals and physicians more than ever need a resource to help them navigate this rapidly evolving field. Editors David Persing and Fred Tenover have brought together a team of experienced researchers and diagnosticians to update this third edition comprehensively, to present the latest developments in molecular diagnostics in the support of clinical care and of basic and clinical research, including next-generation sequencing and whole-genome analysis. These updates are provided in an easy-to-read format and supported by a broad range of practical advice, such as determining the appropriate type and quantity of a specimen, releasing and concentrating the targets, and eliminating inhibitors. Molecular Microbiology: Diagnostic Principles and Practice Presents the latest basic scientific theory underlying molecular diagnostics Offers tested and proven applications of molecular diagnostics for the diagnosis of infectious diseases, including point-of-care testing Illustrates and summarizes key concepts and techniques with detailed figures and tables Discusses emerging technologies, including the use of molecular typing methods for real-time tracking of infectious outbreaks and antibiotic resistance Advises on the latest quality control and quality assurance measures Explores the increasing opportunities and capabilities of information technology Molecular Microbiology: Diagnostic Principles and Practice is a textbook for molecular diagnostics courses that can also be used by anyone involved with diagnostic test selection and interpretation. It is also a useful reference for laboratories and as a continuing education resource for physicians.

Molecular Microbiology Laboratory

Walt Ream,Bruce Geller,Janine Trempy,Katharine G. Field
Molecular Microbiology Laboratory Book PDF

Author : Walt Ream,Bruce Geller,Janine Trempy,Katharine G. Field
Publisher : Elsevier
Page : 268 pages
File Size : 40,7 Mb
Release : 2003-02-28
Category : Science
ISBN : 9780080479514

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Molecular Microbiology Laboratory by Walt Ream,Bruce Geller,Janine Trempy,Katharine G. Field Pdf

Molecular Microbiology Laboratory is designed to teach molecular biology techniques to upper level undergraduates majoring in the life sciences. An extremely detailed lab preparation manual for teaching assistants accompanies the lab book and contains a general discussion of scientific writing and critical reading, as well as detailed instructions for preparation and peer review of lab reports. Each experimental unit is accompanied by a number of additional writing exercises based upon primary journal articles. The studies in these articles employ the techniques that the students are learning in the lab exercises, which reinforces their understanding of the material. These are techniques that students in any biological science will need to know, making this manual applicable to any life science curriculum. Key Features * Not a typical cookbook lab exercise, offers students the excitement and intellectual challenge of characterizing true unknowns. They could discover a new species! * Success rate greater than 85% for the entire experiment, even with very inexperienced students. * The ONLY manual that incorporates writing exercises into the curriculum. * Co-authored by Dr. Janine Trempy, one of four senior editors of the Journal of Microbiology Education, published by the American Society for Microbiology.

Methods for General and Molecular Microbiology

C. A. Reddy,Terry J. Beveridge,John A. Breznak,George Marzluf
Methods for General and Molecular Microbiology Book PDF

Author : C. A. Reddy,Terry J. Beveridge,John A. Breznak,George Marzluf
Publisher : American Society for Microbiology Press
Page : 2737 pages
File Size : 41,9 Mb
Release : 2007-08-17
Category : Science
ISBN : 9781555812232

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Methods for General and Molecular Microbiology by C. A. Reddy,Terry J. Beveridge,John A. Breznak,George Marzluf Pdf

A first source for traditional methods of microbiology as well as commonly used modern molecular microbiological methods. • Provides a comprehensive compendium of methods used in general and molecular microbiology. • Contains many new and expanded chapters, including a section on the newly important field of community and genomic analysis. • Provides step-by-step coverage of procedures, with an extensive list of references to guide the user to the original literature for more complete descriptions. • Presents methods for bacteria, archaea, and for the first time a section on mycology. • Numerous schematics and illustrations (both color and black and white) help the reader to easily understand the topics presented.

Food Molecular Microbiology

Spiros Paramithiotis,Jayanta K Patra
Food Molecular Microbiology Book PDF

Author : Spiros Paramithiotis,Jayanta K Patra
Publisher : CRC Press
Page : 229 pages
File Size : 46,9 Mb
Release : 2019-02-14
Category : Science
ISBN : 9781351615099

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Food Molecular Microbiology by Spiros Paramithiotis,Jayanta K Patra Pdf

With the advances in the field of molecular biology, new tools make it possible to conduct in-depth studies in food microbial communities from a molecular perspective. Information from genomic, transcriptomic, proteomic and metabolomic studies can be integrated through bioinformatic applications, thereby improving our understanding of the interactions between biotic and abiotic factors and concomitantly the physiology of starter cultures, spoilage and pathogenic microbiota. Improvements in the speed, accuracy and reliability of food quality and safety assessment have made the foundation stronger for future developments including the exploitation of gene networks and applications of nanotechnology and systems biology. This book reviews all these developments, provides an integrated view of the subject and helps in identifying areas of future development.

Molecular Wine Microbiology

Alfonso V. Carrascosa Santiago,Rosario Munoz,Ramon Gonzalez Garcia
Molecular Wine Microbiology Book PDF

Author : Alfonso V. Carrascosa Santiago,Rosario Munoz,Ramon Gonzalez Garcia
Publisher : Academic Press
Page : 372 pages
File Size : 53,6 Mb
Release : 2011-05-23
Category : Technology & Engineering
ISBN : 0080962580

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Molecular Wine Microbiology by Alfonso V. Carrascosa Santiago,Rosario Munoz,Ramon Gonzalez Garcia Pdf

Molecular Wine Microbiology features rigorous scientific content written at a level comprehensible for wine professionals as well as advanced students. It includes information on production and spoilage issues, the microbial groups relevant for wine production and microbial wine safety. Microbiology has long been recognized as a key tool in studying wine production, however only recently have wine microbiology studies been addressed at a molecular level, increasing the understanding of how microbiology impacts not only the flavor quality of the wine, but also its safety. Understanding, at a molecular level, how a starter culture can impact ethanol, glycerol, volatile phenols, mannoproteins, biogenic amines or ochratoxin A of a wine are just some of the core points that must be considered in order to achieve maximium consumer acceptability while addressing safety concerns during processing and storage. While other books offer insights into the technological aspects of enology, this book is written by expert microbiologists, who explore the positive and negative impacts of gene function in the production of wine, from a microbiological point of view. Winner of the 2012 Jury Award in Enology from the International Organisation of Vine and Wine Presents the most current methods of studying the microbiology of wine Includes latest identification and typing methods, reducing identification time from days and weeks to minutes and hours Provides important knowledge about the impact of microbiological factors at the molecular level for reduction of wine spoilage and increased wine quality and safety

Type IV Secretion in Gram-Negative and Gram-Positive Bacteria

Steffen Backert,Elisabeth Grohmann
Type IV Secretion in Gram Negative and Gram Positive Bacteria Book PDF

Author : Steffen Backert,Elisabeth Grohmann
Publisher : Springer
Page : 345 pages
File Size : 54,6 Mb
Release : 2018-03-13
Category : Medical
ISBN : 9783319752419

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Type IV Secretion in Gram-Negative and Gram-Positive Bacteria by Steffen Backert,Elisabeth Grohmann Pdf

Type IV secretion systems (T4SSs) are highly versatile membrane-associated transporter machines used by Gram-negative and Gram-positive bacteria to deliver substrate molecules to a large variety of target cells. This volume summarizes our current knowledge of the large variety and structural diversity of T4SSs in pathogenic Escherichia, Agrobacterium, Legionella, Coxiella, Bartonella, Helicobacter, Enterococcus and other species. Divided into 13 chapters contributed by leading experts, it presents findings that significantly enhance our understanding of how various pathogens manipulate host cell functions to trigger bacterial uptake, promote intracellular growth, suppress defense mechanisms and of how bacteria spread antibiotic resistances, thus facilitating bacterial colonization and disease development. The book is an invaluable source of information for researchers and clinicians.

Regulating with RNA in Bacteria and Archaea

Gisela Storz,Kai Papenfort
Regulating with RNA in Bacteria and Archaea Book PDF

Author : Gisela Storz,Kai Papenfort
Publisher : John Wiley & Sons
Page : 400 pages
File Size : 54,5 Mb
Release : 2020-07-10
Category : Medical
ISBN : 9781683670247

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Regulating with RNA in Bacteria and Archaea by Gisela Storz,Kai Papenfort Pdf

Revealing the many roles of RNA in regulating gene expression For decades after the discoveries of messenger RNA, transfer RNA, and ribosomal RNA, it was largely assumed that the role of RNA in the cell was limited to shuttling the genomic message, chaperoning amino acids, and toiling in the ribosomes. Eventually, hints that RNA molecules might have regulatory roles began to appear. With the advent of genomics and bioinformatics, it became evident that numerous other RNA forms exist and have specific functions, including small RNAs (sRNA), RNA thermometers, and riboswitches to regulate core metabolic pathways, bacterial pathogenesis, iron homeostasis, quorum sensing, and biofilm formation. All of these functions, and more, are presented in Regulating with RNA in Bacteria and Archaea, written by RNA biologists from around the globe. Divided into eight sections-RNases and Helicases, Cis-Acting RNAs, Cis Encoded Base Pairing RNAs, Trans-Encoded Base Pairing RNAs, Protein Titration and Scaffolding, General Considerations, Emerging Topics, and Resources-this book serves as an excellent resource for established RNA biologists and for the many scientists who are studying regulated cellular systems. It is no longer a fair assumption that gene expression regulation is the provenance of proteins only or that control is exerted primarily at the level of transcription. This book makes clear that regulatory RNAs are key partners along with proteins in controlling the complex interactions and pathways found within prokaryotes.

Microbiology and Molecular Diagnosis in Pathology

Audrey Wanger,Violeta Chavez,Richard Huang,Amer Wahed,Amitava Dasgupta,Jeffrey K. Actor
Microbiology and Molecular Diagnosis in Pathology Book PDF

Author : Audrey Wanger,Violeta Chavez,Richard Huang,Amer Wahed,Amitava Dasgupta,Jeffrey K. Actor
Publisher : Elsevier
Page : 304 pages
File Size : 42,6 Mb
Release : 2017-06-13
Category : Medical
ISBN : 9780128092385

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Microbiology and Molecular Diagnosis in Pathology by Audrey Wanger,Violeta Chavez,Richard Huang,Amer Wahed,Amitava Dasgupta,Jeffrey K. Actor Pdf

Microbiology and Molecular Diagnosis in Pathology: A Comprehensive Review for Board Preparation, Certification and Clinical Practice reviews all aspects of microbiology and molecular diagnostics essential to successfully passing the American Board of Pathology exam. This review book will also serve as a first resource for residents who want to become familiar with the diagnostic aspects of microbiology and molecular methods, as well as a refresher course for practicing pathologists. Opening chapters discuss issues of laboratory management, including quality control, biosafety, regulations, and proper handling and reporting of laboratory specimens. Review chapters give a quick overview of specific clinical infections as well as different types of bacteria, viruses, fungal infections, and infections caused by parasites. Following these, coverage focuses on diagnostic tools and specific tests: media for clinical microbiology, specific stains and tests for microbial identifications, susceptibility testing and use of antimicrobial agents, tests for detecting antibodies, antigens, and microbial infections. Two final chapters offer overviews on molecular diagnostics principles and methods as well as the application of molecular diagnostics in clinical practice. Takes a practical and easy-to-read approach to understanding microbiology at an appropriate level for both board preparation as well as a professional refresher course Covers all important clinical information found in larger textbooks in a more succinct and easy-to-understand manner Covers essential concepts in microbiology in such a way that residents, fellows, and clinicians understand the methods and tests without having to become specialists in the field Offers a quick overview of specific clinical infections as well as different types of bacteria, viruses, fungal infections, and infections caused by parasites

Diagnostic Molecular Microbiology

David H. Persing
Diagnostic Molecular Microbiology Book PDF

Author : David H. Persing
Publisher : ASM Press
Page : 668 pages
File Size : 52,6 Mb
Release : 1993
Category : Communicable diseases
ISBN : UOM:39015038159748

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Diagnostic Molecular Microbiology by David H. Persing Pdf

Diagnostic Molecular Microbiology is the first major text to provide complete coverage of both the principles and applications of molecular diagnostic methods as they pertain to infectious diseases. Written and edited by leading international experts, this text provides both the theoretical and practical framework for understanding the powerful uses of nucleic acid amplification technologies and for applying these techniques to the rapid detection and characterisation of microbial pathogens (bacterial, viral, fungal, parasitic) in the clinical laboratory. The nine chapters in part 1: Principles summarise the basic theory underlying the emerging discipline of molecular diagnostics. The sixty–six protocols in part 2: Applications, offer proven applications of molecular diagnostic techniques for the diagnosis of infectious diseases. Written in the tradition of ASM′s other classic manuals, this book provides a valuable reference and teaching tool for any clinical microbiology laboratory.

Molecular Microbiology of Heavy Metals

Dietrich H. Nies,Simon Silver
Molecular Microbiology of Heavy Metals Book PDF

Author : Dietrich H. Nies,Simon Silver
Publisher : Springer Science & Business Media
Page : 455 pages
File Size : 48,7 Mb
Release : 2007-03-24
Category : Science
ISBN : 9783540697718

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Molecular Microbiology of Heavy Metals by Dietrich H. Nies,Simon Silver Pdf

This book covers allocation of metals in cells, metal transporter, storage and metalloregulatory proteins, cellular responses to metal ion stress, transcription of genes involved in metal ion homeostasis, uptake of essential metals, metal efflux and other detoxification mechanisms. The book also discusses metal bioreporters for the nanomolar range of concentration and tools to address the metallome. In addition, coverage details specific metals.

Molecular Microbiology

Stephen J.W. Busby,Christopher M. Thomas,Nigel L. Brown
Molecular Microbiology Book PDF

Author : Stephen J.W. Busby,Christopher M. Thomas,Nigel L. Brown
Publisher : Springer Science & Business Media
Page : 330 pages
File Size : 49,9 Mb
Release : 2013-06-29
Category : Science
ISBN : 9783642720710

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Molecular Microbiology by Stephen J.W. Busby,Christopher M. Thomas,Nigel L. Brown Pdf

Molecular microbiology is a rapidly expanding area of contemporary science: the application of molecular biology has opened up the microbial world in many remarkable ways. The attraction of microbes is that they are self-contained and that they offer complete solutions to understanding the phenomenon of life. This book provides a concise introduction to current research in the field. Four major areas are introduced and explained: - Bacterial Biochemistry - Bacterial Genomes - Gene Expression - Microbial Cell Biology

Molecular Medical Microbiology

Yi-Wei Tang,Musa Hindiyeh,Dongyou Liu,Andrew Sails,Paul Spearman,Jing-Ren Zhang
Molecular Medical Microbiology Book PDF

Author : Yi-Wei Tang,Musa Hindiyeh,Dongyou Liu,Andrew Sails,Paul Spearman,Jing-Ren Zhang
Publisher : Academic Press
Page : 3535 pages
File Size : 46,6 Mb
Release : 2023-11-21
Category : Science
ISBN : 9780323899925

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Molecular Medical Microbiology by Yi-Wei Tang,Musa Hindiyeh,Dongyou Liu,Andrew Sails,Paul Spearman,Jing-Ren Zhang Pdf

Molecular Medical Microbiology, Third Edition presents the latest release in what is considered to be the first book to synthesize new developments in both molecular and clinical research. The molecular age has brought about dramatic changes in medical microbiology, along with great leaps in our understanding of the mechanisms of infectious disease. This third edition is completely updated, reviewed and expanded, providing a timely and helpful update for microbiologists, students and clinicians in the era of increasing use of molecular techniques, changing epidemiology and prevalence, and increasing resistance of many pathogenic bacteria. Written by experts in the field, chapters include cutting-edge information and clinical overviews for each major bacterial group, along with the latest updates on vaccine development, molecular technology and diagnostic technology. Completely updated and revised edition of this comprehensive and accessible reference on molecular medical microbiology Includes full color presentations throughout Delves into in-depth discussions on individual pathogenic bacteria in a system-oriented approach Includes a clinical overview for each major bacterial group Presents the latest information on vaccine development, molecular technology and diagnostic technology Provides more than 100 chapters on all major groups of bacteria

Advanced Techniques in Diagnostic Microbiology

Yi-Wei Tang,Charles W. Stratton
Advanced Techniques in Diagnostic Microbiology Book PDF

Author : Yi-Wei Tang,Charles W. Stratton
Publisher : Springer Science & Business Media
Page : 540 pages
File Size : 53,6 Mb
Release : 2007-01-16
Category : Medical
ISBN : 9780387328928

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Advanced Techniques in Diagnostic Microbiology by Yi-Wei Tang,Charles W. Stratton Pdf

Clinical microbiologists are engaged in the field of diagnostic microbiology to determine whether pathogenic microorganisms are present in clinical specimens collected from patients with suspected infections. If microorganisms are found, these are identified and susceptibility profiles, when indicated, are determined. During the past two decades, technical advances in the field of diagnostic microbiology have made constant and enormous progress in various areas, including bacteriology, mycology, mycobacteriology, parasitology, and virology. The diagnostic capabilities of modern clinical microbiology laboratories have improved rapidly and have expanded greatly due to a technological revolution in molecular aspects of microbiology and immunology. In particular, rapid techniques for nucleic acid amplification and characterization combined with automation and user-friendly software have significantly broadened the diagnostic arsenal for the clinical microbiologist. The conventional diagnostic model for clinical microbiology has been labor-intensive and frequently required days to weeks before test results were available. Moreover, due to the complexity and length of such testing, this service was usually directed at the hospitalized patient population. The physical structure of laboratories, staffing patterns, workflow, and turnaround time all have been influenced profoundly by these technical advances. Such changes will undoubtedly continue and lead the field of diagnostic microbiology inevitably to a truly modern discipline. Advanced Techniques in Diagnostic Microbiology provides a comprehensive and up-to-date description of advanced methods that have evolved for the diagnosis of infectious diseases in the routine clinical microbiology laboratory. The book is divided into two sections. The first techniques section covers the principles and characteristics of techniques ranging from rapid antigen testing, to advanced antibody detection, to in vitro nucleic acid amplification techniques, and to nucleic acid microarray and mass spectrometry. Sufficient space is assigned to cover different nucleic acid amplification formats that are currently being used widely in the diagnostic microbiology field. Within each technique, examples are given regarding its application in the diagnostic field. Commercial product information, if available, is introduced with commentary in each chapter. If several test formats are available for a technique, objective comparisons are given to illustrate the contrasts of their advantages and disadvantages. The second applications section provides practical examples of application of these advanced techniques in several "hot" spots in the diagnostic field. A diverse team of authors presents authoritative and comprehensive information on sequence-based bacterial identification, blood and blood product screening, molecular diagnosis of sexually transmitted diseases, advances in mycobacterial diagnosis, novel and rapid emerging microorganism detection and genotyping, and future directions in the diagnostic microbiology field. We hope our readers like this technique-based approach and your feedback is highly appreciated. We want to thank the authors who devoted their time and efforts to produce their chapters. We also thank the staff at Springer Press, especially Melissa Ramondetta, who initiated the whole project. Finally, we greatly appreciate the constant encouragement of our family members through this long effort. Without their unwavering faith and full support, we would never have had the courage to commence this project.

PCR for Clinical Microbiology

Ian W.J. Carter,Margret Schuller,Gregory S. James,Theo P. Sloots,Catriona L. Halliday
PCR for Clinical Microbiology Book PDF

Author : Ian W.J. Carter,Margret Schuller,Gregory S. James,Theo P. Sloots,Catriona L. Halliday
Publisher : Springer Science & Business Media
Page : 438 pages
File Size : 50,9 Mb
Release : 2010-07-03
Category : Science
ISBN : 9789048190393

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PCR for Clinical Microbiology by Ian W.J. Carter,Margret Schuller,Gregory S. James,Theo P. Sloots,Catriona L. Halliday Pdf

Not another textbook, but a valuable tool for doctors and microbiologists wanting to know how to set up a PCR diagnostic microbiology laboratory according to current regulatory standards and perform assays supplied with patient clinical diagnostic criteria and easy to follow protocols. Whether laboratories are using commercial kits or in-house methods developed in their own laboratories or adopted from published methods, all clinical microbiology laboratories need to be able to understand, critically evaluate, perform and interpret these tests according to rigorous and clinically appropriate standards and international guidelines. The cost and effort of development and evaluation of in-house tests is considerable and many laboratories do not have the resources to do so. This compendium is a vehicle to improve and maintain the clinical relevance and high quality of diagnostic PCR. It is a unique collection of; guidelines for PCR laboratory set up and quality control, test selection criteria, methods and detailed step by step protocols for a diagnostic assays in the field of molecular microbiology. The structure of the book provides the PCR fundamentals and describes the clinical aspects and diagnosis of infectious disease. This is followed by protocols divided into; bacteria, virus, fungi and parasites, and susceptibility screens. The inclusion of medical criteria and interpretation adds value to the compendium and benefits clinicians, scientists, researchers and students of clinical diagnostic microbiology

Molecular Food Microbiology

Dongyou Liu
Molecular Food Microbiology Book PDF

Author : Dongyou Liu
Publisher : CRC Press
Page : 525 pages
File Size : 44,6 Mb
Release : 2021-04-11
Category : Medical
ISBN : 9781351120371

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Molecular Food Microbiology by Dongyou Liu Pdf

The elucidation of DNA double helix in 1953 and the publication of DNA cloning protocol in 1973 have put wings under the sail of molecular biology, which has since quietly revolutionized many fields of biological science, including food microbiology. Exploiting the power and versatility of molecular technologies, molecular food microbiology extends and greatly improves on phenotypically based food microbiology, leading to the development of better diagnostics for foodborne infections and intoxications, and contributing to the design of more effective therapeutics and prophylaxes against foodborne diseases. Forming part of the Food Microbiology series, Molecular Food Microbiology provides a state of art coverage on molecular techniques applicable to food microbiology. While the introductory chapter contains an overview on the principles of current DNA, RNA and protein techniques and discusses their utility in helping solve practical problems that food microbiology is facing now and in the future, the remaining chapters present detailed moleuclar analyses of selective foodborne viruses, bacteria, fungi and parasites. Key Features: Contains a state of art overview on moleuclar techniques applicable to food microbiology research and development Presents in-depth molecular analysis of selective foodborne viruses, bacteria, fungi and parasites Highlights the utility of molecular tecniques for accurate diagnosis and effective control of foodborne diseases Includes expert contributions from international scientists involved in molecular food microbiology research Represents a highly informative textbook for students majoring in food, medical, and veterinary microbiology Offers a contemporary reference for scholars and educators wishing to keep abreast with the latest developments in molecular food microbiology With contributions from international scientists involved in molecular food microbiology research, this book constitutes an informative textbook for undergraduates and postgraduates majoring in food, medical, and veterinary microbiology; represents an indispensable guide for food, medical, and veterinary scientists engaged in molecular food microbiology research and development; and offers a contemporary update for scholars and educators trying to keep in touch with the latest developments in molecular food microbiology.