Pcr For Clinical Microbiology

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PCR for Clinical Microbiology

Ian W.J. Carter,Margret Schuller,Gregory S. James,Theo P. Sloots,Catriona L. Halliday
PCR for Clinical Microbiology Book PDF

Author : Ian W.J. Carter,Margret Schuller,Gregory S. James,Theo P. Sloots,Catriona L. Halliday
Publisher : Springer Science & Business Media
Page : 420 pages
File Size : 47,7 Mb
Release : 2010-07-03
Category : Science
ISBN : 9789048190393

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PCR for Clinical Microbiology by Ian W.J. Carter,Margret Schuller,Gregory S. James,Theo P. Sloots,Catriona L. Halliday Pdf

Not another textbook, but a valuable tool for doctors and microbiologists wanting to know how to set up a PCR diagnostic microbiology laboratory according to current regulatory standards and perform assays supplied with patient clinical diagnostic criteria and easy to follow protocols. Whether laboratories are using commercial kits or in-house methods developed in their own laboratories or adopted from published methods, all clinical microbiology laboratories need to be able to understand, critically evaluate, perform and interpret these tests according to rigorous and clinically appropriate standards and international guidelines. The cost and effort of development and evaluation of in-house tests is considerable and many laboratories do not have the resources to do so. This compendium is a vehicle to improve and maintain the clinical relevance and high quality of diagnostic PCR. It is a unique collection of; guidelines for PCR laboratory set up and quality control, test selection criteria, methods and detailed step by step protocols for a diagnostic assays in the field of molecular microbiology. The structure of the book provides the PCR fundamentals and describes the clinical aspects and diagnosis of infectious disease. This is followed by protocols divided into; bacteria, virus, fungi and parasites, and susceptibility screens. The inclusion of medical criteria and interpretation adds value to the compendium and benefits clinicians, scientists, researchers and students of clinical diagnostic microbiology

Rapid Cycle Real-Time PCR — Methods and Applications

U. Reischl,C. Wittwer,F. Cockerill
Rapid Cycle Real Time PCR Methods and Applications Book PDF

Author : U. Reischl,C. Wittwer,F. Cockerill
Publisher : Springer Science & Business Media
Page : 253 pages
File Size : 50,6 Mb
Release : 2012-12-06
Category : Science
ISBN : 9783642483516

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Rapid Cycle Real-Time PCR — Methods and Applications by U. Reischl,C. Wittwer,F. Cockerill Pdf

Rapid Cycle Real-Time PCR is a powerful analytical tool with broad application for the basic and applied life sciences. Compared with conventional PCR technology, Rapid Cycle Real-Time PCR is faster, has greater specificity, and is more easily adaptable for a variety of diagnostic tests, including qualitative, quantitative and mutation detection assays. This book provides general overviews of this technology for use in the clinical microbiology laboratory as well as specific diagnostic protocols for the detection of viral, bacterial and fungal pathogens and genetically modified organisms in human specimens and foodstuffs. All of these protocols have been developed, verified, and validated by experts in the field and should be of great interest for clinical microbiologists, pathologists, laboratory technologists as well as practicing physicians.

DNA Methods in Clinical Microbiology

P. Singleton
DNA Methods in Clinical Microbiology Book PDF

Author : P. Singleton
Publisher : Springer Science & Business Media
Page : 262 pages
File Size : 48,6 Mb
Release : 2013-04-17
Category : Medical
ISBN : 9789401712866

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DNA Methods in Clinical Microbiology by P. Singleton Pdf

DNA Methods in Clinical Microbiology describes the novel DNA-based technology now used in the diagnosis and management of infectious diseases. It is a concise, yet readable, overview written primarily for clinicians, clinical microbiologists, medical students and undergraduates in medical and veterinary microbiology. The book has two primary aims. First, to explain the principles of these methods at the `molecular' level. Second, to provide a clinical perspective by reporting results from actual DNA-based investigations on a range of specimens. Those approaching DNA methods for the first time are assisted by a brief résumé of the relevant features of nucleic acids (Chapter 2): this information is essential for an understanding of later chapters. Subsequent text covers detection, characterization and quantification of pathogens by a variety of methods - e.g., target amplification (PCR, LCR, NASBA, TMA and SDA), signal amplification (bDNA) and probe-based techniques; the chapter on typing describes nearly twenty named molecular methods, including spoligotyping and MLST. All chapters include an adequate range of current reference from which, if required, detailed protocols can be obtained. The diagrams are clear, and readers are assisted by a detailed index.

Clinical Microbilogy

Charles W Stratton, MD
Clinical Microbilogy Book PDF

Author : Charles W Stratton, MD
Publisher : Demos Medical Publishing
Page : 164 pages
File Size : 41,7 Mb
Release : 2011-07-10
Category : Medical
ISBN : 9781617050480

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Clinical Microbilogy by Charles W Stratton, MD Pdf

About the Diagnostic Standards of Care Series A key issue for every laboratory and individual laboratory practitioner is the assessment of risk and a current working knowledge of the standards of care established for diagnostic testing via guidelines, major studies and trials. The Diagnostic Standards of Care series presents an overview of the key diagnoses in clinical pathology using case examples to illustrate effective analysis of the case in light of current evidence and standards for the problem discussed. In addition to being practical diagnostic guides, these volumes will have a unique emphasis on quality assurance and evidence-based testing practices and the role of the pathologist in ensuring quality and patient safety. Clinical Microbiology addresses common medical errors seen in the clinical microbiology laboratory in order to show these errors to pathologists and laboratory technicians as well as clinicians. The goal is to allow such errors to be corrected by both individual effort and a systems approach in the laboratory. The book addresses potential medical errors in test ordering and specimen collection, testing in the laboratory, and reporting and interpretation of test results. Each of these phases can have an adverse impact on the diagnosis and treatment of an infectious disease if a medical error occurs. Potential medical errors are described and discussed in a clinical case-based learning format to effectively illustrate the conditions that contribute to these errors and enable the reader to recognize and avoid them in daily practice. Clinical Microbiology Features Descriptions of potential errors in test ordering and specimen collection in clinical microbiology Descriptions of potential errors in test performance in clinical microbiology Descriptions of potential errors in test reporting and interpretation in clinical microbiology Clinical case discussions provide "real world" illustrations of potential errors and how to anticipate and avoid them in practice Is pocket-sized for portability

Rapid Cycle Real-Time PCR — Methods and Applications

Udo Reischl,Carl Wittwer,F. Cockerill
Rapid Cycle Real Time PCR Methods and Applications Book PDF

Author : Udo Reischl,Carl Wittwer,F. Cockerill
Publisher : Springer
Page : 258 pages
File Size : 54,5 Mb
Release : 2012-12-12
Category : Science
ISBN : 3642483534

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Rapid Cycle Real-Time PCR — Methods and Applications by Udo Reischl,Carl Wittwer,F. Cockerill Pdf

Rapid Cycle Real-Time PCR is a powerful analytical tool with broad application for the basic and applied life sciences. Compared with conventional PCR technology, Rapid Cycle Real-Time PCR is faster, has greater specificity, and is more easily adaptable for a variety of diagnostic tests, including qualitative, quantitative and mutation detection assays. This book provides general overviews of this technology for use in the clinical microbiology laboratory as well as specific diagnostic protocols for the detection of viral, bacterial and fungal pathogens and genetically modified organisms in human specimens and foodstuffs. All of these protocols have been developed, verified, and validated by experts in the field and should be of great interest for clinical microbiologists, pathologists, laboratory technologists as well as practicing physicians.

Clinical Microbiology Procedures Handbook

Anonim
Clinical Microbiology Procedures Handbook Book PDF

Author : Anonim
Publisher : John Wiley & Sons
Page : 2954 pages
File Size : 45,9 Mb
Release : 2020-08-06
Category : Medical
ISBN : 9781555818814

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Clinical Microbiology Procedures Handbook by Anonim Pdf

In response to the ever-changing needs and responsibilities of the clinical microbiology field, Clinical Microbiology Procedures Handbook, Fourth Edition has been extensively reviewed and updated to present the most prominent procedures in use today. The Clinical Microbiology Procedures Handbook provides step-by-step protocols and descriptions that allow clinical microbiologists and laboratory staff personnel to confidently and accurately perform all analyses, including appropriate quality control recommendations, from the receipt of the specimen through processing, testing, interpretation, presentation of the final report, and subsequent consultation.

Clinical Microbiology for Diagnostic Laboratory Scientists

Sarah J. Pitt
Clinical Microbiology for Diagnostic Laboratory Scientists Book PDF

Author : Sarah J. Pitt
Publisher : John Wiley & Sons
Page : 307 pages
File Size : 52,5 Mb
Release : 2018-01-16
Category : Science
ISBN : 9781118745854

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Clinical Microbiology for Diagnostic Laboratory Scientists by Sarah J. Pitt Pdf

A modern, evaluative, and integrative approach to diagnostic microbiology encouraging problem-solving in the clinical laboratory context through the use of examples to illustrate clinical and diagnostic issues Clinical Microbiology for Diagnostic Laboratory Scientists is designed to encourage readers to develop a way of thinking that can be applied to any diagnostic scenario in microbiology. Through consideration of a selected range of infections caused by pathogenic bacteria, viruses, fungi, protozoa, and helminths, the book encourages readers to explore connections between the available information about clinical symptoms, pathogenesis of infections, and the approaches used in laboratory diagnosis, in order to develop new insights. The book begins with an introductory chapter that outlines the scope of clinical diagnostic microbiology and the key areas for the laboratory scientist to be aware of. The subsequent six chapters review a type of infection in depth, using particular pathogenic microorganisms to illustrate salient points. At the end of each chapter there are three exercises related to management of a diagnostic service and assessing the suitability of test methods to specific contexts. There are no right or wrong answers to these, but the reader can discuss them with their laboratory colleagues or university tutor. Makes extensive use of published research in the form of journal articles, publically available epidemiological data, professional guidelines, and specialist websites Stimulates the reader in critical appraisal of published evidence and encourages problem-solving in the laboratory Outlines the scope of clinical diagnostic microbiology and the key areas for the laboratory scientist to be aware of Considers topics relevant to professional scientists working in the area of diagnostic microbiology Clinical Microbiology for Diagnostic Laboratory Scientists is ideal for post graduate scientists intending to pursue careers in diagnostic clinical microbiology and for biomedical scientists, clinical scientists, and full time students studying for upper level qualifications in biomedical science, microbiology, or virology.

Practical Medical Microbiology for Clinicians

Frank E. Berkowitz,Robert C. Jerris
Practical Medical Microbiology for Clinicians Book PDF

Author : Frank E. Berkowitz,Robert C. Jerris
Publisher : John Wiley & Sons
Page : 480 pages
File Size : 46,8 Mb
Release : 2016-02-15
Category : Science
ISBN : 9781119066743

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Practical Medical Microbiology for Clinicians by Frank E. Berkowitz,Robert C. Jerris Pdf

Infectious diseases constitute a major portion of illnesses worldwide, and microbiology is a main pillar of clinical infectious disease practice. Knowledge of viruses, bacteria, fungi, and parasites is integral to practice in clinical infectious disease. Practical Medical Microbiology is an invaluable reference for medical microbiology instructors. Drs. Berkowitz and Jerris are experienced teachers in the fields of infectious diseases and microbiology respectively, and provide expert insight into microorganisms that affect patients, how organisms are related to each other, and how they are isolated and identified in the microbiology laboratory. The text also is designed to provide clinicians the knowledge they need to facilitate communication with the microbiologist in their laboratory. The text takes a systematic approach to medical microbiology, describing taxonomy of human pathogens and consideration of organisms within specific taxonomic groups. The text tackles main clinical infections caused by different organisms, and supplements these descriptions with clinical case studies, in order to demonstrate the effects of various organisms. Practical Medical Microbiology is an invaluable resource for students, teachers, and researchers studying clinical microbiology, medical microbiology, infectious diseases, and virology.

Advanced Techniques in Diagnostic Microbiology

Yi-Wei Tang,Charles W. Stratton
Advanced Techniques in Diagnostic Microbiology Book PDF

Author : Yi-Wei Tang,Charles W. Stratton
Publisher : Springer
Page : 760 pages
File Size : 53,6 Mb
Release : 2018-11-09
Category : Medical
ISBN : 9783319951119

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Advanced Techniques in Diagnostic Microbiology by Yi-Wei Tang,Charles W. Stratton Pdf

In recent years, advanced molecular techniques in diagnostic microbiology have been revolutionizing the practice of clinical microbiology in the hospital setting. Molecular diagnostic testing in general and nucleic acid-based amplification methods in particular have been heralded as diagnostic tools for the new millennium. This third edition covers not only the most recent updates and advances, but details newly invented omic techniques, such as next generation sequencing. It is divided into two distinct volumes, with Volume 1 describing the techniques, and Volume 2 addressing their applications in the field. In addition, both volumes focus more so on the clinical relevance of the test results generated by these techniques than previous editions.

Real-time PCR in Microbiology

Ian Maxwell Mackay
Real time PCR in Microbiology Book PDF

Author : Ian Maxwell Mackay
Publisher : Caister Academic Press
Page : 0 pages
File Size : 43,8 Mb
Release : 2007
Category : Medical
ISBN : 1904455182

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Real-time PCR in Microbiology by Ian Maxwell Mackay Pdf

Real-time PCR has established itself as a sensitive and specific qualitative and quantitative technique that has become important to all areas of microbiology. This invaluable book describes and explains some of the more complex aspects of real-time PCR presenting a background for the novice, a theoretical reference for the experienced user, and useful discussions of future developments. Chapters address the basics of PCR history, oligonucleotide design, target preparation, standardisation, quantification, various applications, and future challenges. The final chapter is presented in the format of a roundtable discussion providing an insightful, topical and interesting discourse with contributions from over 30 authorities and experts on real-time PCR. The editor and authors have produced an excellent book that will be extremely useful for all microbiologists. It is a recommended book for all microbiology laboratories.

Advanced Techniques in Diagnostic Microbiology

Yi-Wei Tang,Charles W. Stratton
Advanced Techniques in Diagnostic Microbiology Book PDF

Author : Yi-Wei Tang,Charles W. Stratton
Publisher : Springer
Page : 541 pages
File Size : 55,5 Mb
Release : 2018-11-09
Category : Medical
ISBN : 9783319339009

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Advanced Techniques in Diagnostic Microbiology by Yi-Wei Tang,Charles W. Stratton Pdf

In recent years, advanced molecular techniques in diagnostic microbiology have been revolutionizing the practice of clinical microbiology in the hospital setting. Molecular diagnostic testing in general and nucleic acid-based amplification methods in particular have been heralded as diagnostic tools for the new millennium. This third edition covers not only the most recent updates and advances, but details newly invented omic techniques, such as next generation sequencing. It is divided into two distinct volumes, with Volume 1 describing the techniques, and Volume 2 addressing their applications in the field. In addition, both volumes focus more so on the clinical relevance of the test results generated by these techniques than previous editions.

Medical Microbiology

Michael Ford
Medical Microbiology Book PDF

Author : Michael Ford
Publisher : Unknown
Page : 565 pages
File Size : 40,7 Mb
Release : 2019-06-05
Category : Electronic
ISBN : 9780198818144

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Medical Microbiology by Michael Ford Pdf

Biomedical scientists are the foundation of modern healthcare, from cancer screening to diagnosing HIV, from blood transfusion for surgery to food poisoning and infection control. Without biomedical scientists, the diagnosis of disease, the evaluation of the effectiveness of treatment, andresearch into the causes and cures of disease would not be possible.The Fundamentals of Biomedical Science series has been written to reflect the challenges of practicing biomedical science today. It draws together essential basic science with insights into laboratory practice to show how an understanding of the biology of disease is coupled to the analyticalapproaches that lead to diagnosis. Assuming only a minimum of prior knowledge, the series reviews the full range of disciplines to which a Biomedical Scientist may be exposed - from microbiology to cytopathology to transfusion science.The series:- Understands the complex roles of Biomedical Scientists in the modern practice of medicine.- Understands the development needs of employers and the Profession.- Addresses the need for understanding of a range of fundamental sciences in the context of Biomedicine.- Places the theoretical aspects of Biomedical Science in their practical context via clinical case studies.Medical Microbiology covers a range of key laboratory techniques used in the diagnosis of important human diseases caused by microorganisms. From sample collection, through to analysis and laboratory investigation, the text covers a wide range of procedures and highlights how and why results aregenerated. The third edition has been expanded to cover a wider range of topics, including a new chapter on Whole Genome Sequencing and extended coverage of syphilis and MALDI.

Molecular Methods for Microbial Identification and Typing

K.J. Towner,A. Cockayne
Molecular Methods for Microbial Identification and Typing Book PDF

Author : K.J. Towner,A. Cockayne
Publisher : Springer Science & Business Media
Page : 208 pages
File Size : 40,7 Mb
Release : 2013-03-07
Category : Science
ISBN : 9789401115063

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Molecular Methods for Microbial Identification and Typing by K.J. Towner,A. Cockayne Pdf

The accurate identification and typing of microbes is essential for workers active in all fields of microbiology. Many examples of modern molecular methods have been concealed in scientific and medical literature but this introductory text considers the possible applications of such methods and compares their advantages and disadvantages.

Advanced Techniques in Diagnostic Microbiology

Yi-Wei Tang,Charles W. Stratton
Advanced Techniques in Diagnostic Microbiology Book PDF

Author : Yi-Wei Tang,Charles W. Stratton
Publisher : Springer Science & Business Media
Page : 943 pages
File Size : 55,8 Mb
Release : 2012-09-13
Category : Medical
ISBN : 9781461439691

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Advanced Techniques in Diagnostic Microbiology by Yi-Wei Tang,Charles W. Stratton Pdf

In the United States, hospitals annually report over 5 million cases of infectious-disease-related illnesses: clinical microbiology laboratories in these hospitals are engaged in detecting and identifying the pathogenic microorganisms in clinical specimens collected from these patients with suspected infections. Clearly, the timely and accurate detection/identification of these microbial pathogens is critical for patient treatment decisions and outcomes for millions of patients each year. Despite an appreciation that the outcome of an infectious-disease-related illness is directly related to the time required to detect and identify a microbial pathogen, clinical microbiology laboratories in the United States as well as worldwide have long been hampered by traditional culture-based assays, which may require prolonged incubation time for slowly growing microorganisms such as Mycobacterium tuberculosis. Moreover, traditional culture-based assays often require multiple steps with additional time needed for discernment of species and/or detection of antimicrobial resistance. Finally, these traditional, slow multistep culture-based assays are labor-intensive and required skilled clinical microbiologists at the bench. Over the past several decades, advanced molecular techniques in diagnostic microbiology quietly have been revolutionizing the practice of clinical microbiology in the hospital setting. Indeed, molecular diagnostic testing in general and nucleic-acid-based amplification methods in particular have been heralded as diagnostic tools for the new millennium. There is no question that the development of rapid molecular techniques for nucleic acid amplification/characterization combined with automation and user-friendly software has greatly broadened the diagnostic capabilities of the clinical microbiology laboratory. These technical advances in molecular microbiology over the first decade of the 21st Century have profoundly influenced the physical structure of clinical microbiology laboratories as well as their staffing patterns, workflow, and turnaround time. These molecular microbiology advances have also resulted in the need for a revised and updated second edition of Advanced Techniques in Diagnostic Microbiology. This second edition again provides an updated and comprehensive description of the ongoing evolution of molecular methods for the diagnosis of infectious diseases. In addition, many new chapters have been added, including a chapter on the clinical interpretation and relevance of advanced technique results. The second edition, like the first edition, includes both a “techniques” section describing the latest molecular techniques and an “applications” section describing how these advanced molecular techniques are being used in the clinical setting. Finally, the second edition, like the first edition, utilizes a diverse team of authors who have compiled chapters that provide the reader with comprehensive and useable information on advanced molecular microbiology techniques.

Manual of Clinical Microbiology

James Versalovic
Manual of Clinical Microbiology Book PDF

Author : James Versalovic
Publisher : American Society for Microbiology Press
Page : 3227 pages
File Size : 52,8 Mb
Release : 2011-05-16
Category : Medical
ISBN : 9781555814632

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Manual of Clinical Microbiology by James Versalovic Pdf

The most authoritative, comprehensive reference in the field. • Sets the standard for state-of-the-science laboratory practice. • A collaborative effort of 22 editors and more than 260 authors from around the world, all experienced researchers and practitioners in medical and diagnostic microbiology. • Includes 149 chapters of the latest research findings, infectious agents, methods, practices, and safety guidelines. • Indispensable to clinical microbiologists, laboratory technologists, and infectious disease specialists in hospitals, clinics, reference laboratories, and more