Monoclonal Hybridoma Antibodies

Author : National Research Council,Institute for Laboratory Animal Research,Committee on Methods of Producing Monoclonal Antibodies
Publisher : National Academies Press
Page : 74 pages
File Size : 41,8 Mb
Release : 1999-05-06
Category : Medical
ISBN : 9780309173056

Get Book

Monoclonal Antibody Production by National Research Council,Institute for Laboratory Animal Research,Committee on Methods of Producing Monoclonal Antibodies Pdf

The American Anti-Vivisection Society (AAVS) petitioned the National Institutes of Health (NIH) on April 23, 1997, to prohibit the use of animals in the production of mAb. On September 18, 1997, NIH declined to prohibit the use of mice in mAb production, stating that "the ascites method of mAb production is scientifically appropriate for some research projects and cannot be replaced." On March 26, 1998, AAVS submitted a second petition, stating that "NIH failed to provide valid scientific reasons for not supporting a proposed ban." The office of the NIH director asked the National Research Council to conduct a study of methods of producing mAb. In response to that request, the Research Council appointed the Committee on Methods of Producing Monoclonal Antibodies, to act on behalf of the Institute for Laboratory Animal Research of the Commission on Life Sciences, to conduct the study. The 11 expert members of the committee had extensive experience in biomedical research, laboratory animal medicine, animal welfare, pain research, and patient advocacy (Appendix B). The committee was asked to determine whether there was a scientific necessity for the mouse ascites method; if so, whether the method caused pain or distress; and, if so, what could be done to minimize the pain or distress. The committee was also asked to comment on available in vitro methods; to suggest what acceptable scientific rationale, if any, there was for using the mouse ascites method; and to identify regulatory requirements for the continued use of the mouse ascites method. The committee held an open data-gathering meeting during which its members summarized data bearing on those questions. A 1-day workshop (Appendix A) was attended by 34 participants, 14 of whom made formal presentations. A second meeting was held to finalize the report. The present report was written on the basis of information in the literature and information presented at the meeting and the workshop.

Author : Roger H. Kennett
Publisher : Springer Science & Business Media
Page : 423 pages
File Size : 41,9 Mb
Release : 2012-12-06
Category : Medical
ISBN : 9781461575054

Get Book

Monoclonal Antibodies by Roger H. Kennett Pdf

On August 7, 1975, Kohler and Milstein published in Nature (256:495) a report describing "Continuous cultures of fused cells secreting antibody of predefined specificity. " Their report has become a classic and has already had a profound effect on basic and applied research in biology and medicine. By the time the first Workshop on Lymphocyte Hybridomas (Current Topics in Microbiology and Im munology 81, 1978) was held on April 3-5, 1978, in Bethesda, Maryland, investi gators from many laboratories had made hybrids between plasmacytomas and spleen cells from immunized animals and had obtained monoclonal antibodies reacting with a broad variety of antigenic determinants. At the time Kohler and Milstein introduced this new technology, the editors of this volume were involved in the production of antisera against differentiation antigens (K. B. B. ), histocompatibility antigens (T. ]. McK. ), and human tumor associated antigens (R. H. K. ). Because of the potential usefulness of monoclonal antibodies in these areas, we each began production of hybridomas and analysis of the resulting monoclonal reagents. One of the most interesting aspects of participation in the early stages of the development and application of hybrid oma technology has been observing how the implications of the initial observa tions gradually spread first among the practitioners of immunology and immu nogenetics, and then to other areas of the biological sciences, such as developmental biology, biochemistry, human genetics, and cell and tumor biology.

Author : Lawrence B. Schook
Publisher : New York : Dekker
Page : 344 pages
File Size : 54,5 Mb
Release : 1987
Category : Medical
ISBN : UOM:39015011765164

Get Book

Monoclonal Antibody Production Techniques and Applications by Lawrence B. Schook Pdf

Author : J. Eryl Liddell,A. Cryer
Publisher : John Wiley & Sons
Page : 212 pages
File Size : 53,6 Mb
Release : 1991-08-26
Category : Medical
ISBN : 0471929050

Get Book

A Practical Guide to Monoclonal Antibodies by J. Eryl Liddell,A. Cryer Pdf

Includes all of the information required to produce monoclonal antibodies in the laboratory and to prepare them for use in a multitude of given applications. Production procedures are treated in chronological order, beginning with basic tissue culture techniques, immunization strategies and screening test design, followed by production of hybridoma cell lines and basic antibody characterization, purification and labeling. Each chapter contains explanatory text on each step with comparative analysis of methods where appropriate. All necessary experimental protocols are presented in a self-contained format that is easy to follow in the laboratory. Alternative protocols are provided where relevant; for others not included in full, source references are presented. Surveys the current status of human hybridoma production and antibody engineering using molecular biology techniques.

Author : John G.R. Hurrell
Publisher : CRC Press
Page : 239 pages
File Size : 55,9 Mb
Release : 2018-05-04
Category : Health & Fitness
ISBN : 9781351083270

Get Book

Monoclonal Hybridoma Antibodies by John G.R. Hurrell Pdf

The first section of this volume is aimed to provide a comprehensive review of the many varied and often empirically derived techniques and procedures currently in use to produce monoclonal hybridoma cell lines and to characterize the antibodies secreted. The goal has been achieved with the chapter contributed by Zola and Brookes who, as each step in the process of hybridoma production and antibody characterisation is reviewed, have provided an experimental procedure found to be satisfactory in their laboratory.The second section of this volume is designed to provide a review of areas in which monoclonal hybridoma antibodies have been of particular advantage. This is a rapidly advancing field which could not be thoroughly reviewed in a single volume.

Author : Edgar Engleman
Publisher : Springer Science & Business Media
Page : 528 pages
File Size : 54,7 Mb
Release : 2013-11-11
Category : Medical
ISBN : 9781468449495

Get Book

Human Hybridomas and Monoclonal Antibodies by Edgar Engleman Pdf

Soon after Kohler and Milstein described the use of somatic cell hybridization for the production of murine monoclonal antibodies of desired specificity, this relatively simple technique became widely applied. Indeed, production of murine monoclonal antibodies is now considered routine by immunologists and nonimmunologists alike. However, as heterologous proteins, mouse monoclonal antibodies have one major limitation: they are immunogenic in man and, hence, their use in vivo is severely limited. An obvious solution to this problem is to produce human hybridomas with the same techniques used for the production of rodent hybrids. Unfortunately, the history of human hybridomas has been marked by substantive and often exasperating tech nical problems, and the first reports of hybrids secreting human immu noglobulin of desired specificity did not appear until 1980. These reports were met with initial enthusiasm, but it soon became apparent that while human lymphocytes might be fused, their frequency, level of Ig synthesis, and stability were such that production of human antibodies with this method was neither routine nor practical. Nonetheless, a sufficient number of investiga tors persevered, and during the next 5 years relatively efficient B-cell fusion partners as well as improved methods of Epstein-Barr virus transformation were developed. Generation of human T -T hybrids has also been achieved, although problems of chromosomal stability remain a substantial obstacle, more so than with B-cell lines.

Author : Johann H. Peters,Horst Baumgarten
Publisher : Springer Science & Business Media
Page : 504 pages
File Size : 42,6 Mb
Release : 2012-12-06
Category : Medical
ISBN : 9783642745324

Get Book

Monoclonal Antibodies by Johann H. Peters,Horst Baumgarten Pdf

The present new version of this popular laboratory manual is at the same time the first one of this text in the English language - and this makes me even a little proud, as it reminds me of probably the first collection of monoclonal recipes in English, written by myself, which circulated for a couple of years in many laboratories. In the meantime many researchers have put enormous effort into improving methods for monoclonal antibody production. The proce dures have become more and more standardized and by this have more and more lost the character of magic secrets. Hinrich Peters and Horst Baumgarten, who had followed this good tradition already in the previous edition, written in German, suc ceeded in making laboratory tricks teachable. They had contributed their own experiences in cell culture and immunology, and were able to engage a number of experienced authors to contribute to the work. They were all willing to follow the general concept of this book, which contains a brief theoretical background for the methods described and presents the procedures in a highly organized structure. So the book has retained its shape as a "cook-book", which I especially like.

Author : Timothy Springer
Publisher : Springer Science & Business Media
Page : 603 pages
File Size : 55,6 Mb
Release : 2013-11-11
Category : Medical
ISBN : 9781468449648

Get Book

Hybridoma Technology in the Biosciences and Medicine by Timothy Springer Pdf

Author : Geoffrey Josiah Letchworth
Publisher : Unknown
Page : 58 pages
File Size : 43,8 Mb
Release : 1984
Category : Hybridomas
ISBN : UVA:X001754766

Get Book

Methods for Production of Monoclonal Antibodies by Geoffrey Josiah Letchworth Pdf

Author : A.M. Campbell
Publisher : Elsevier
Page : 264 pages
File Size : 44,8 Mb
Release : 2000-04-01
Category : Medical
ISBN : 0080858821

Get Book

Monoclonal Antibody Technology: The Production and Characterization of Rodent and Human Hybridomas by A.M. Campbell Pdf

This volume contains detailed, comprehensive advice on rat, mouse and human hybridoma production. It begins with a general introduction, then describes the practical applications of the technology with photographs and protocols for everything from animal dissection to epitope analysis of antigens.

Author : Committee on Methods of Producing Monoclonal Antibodies,Institute for Laboratory Animal Research,National Research Council
Publisher : National Academies Press
Page : 74 pages
File Size : 49,5 Mb
Release : 1999-05-20
Category : Science
ISBN : 9780309519045

Get Book

Monoclonal Antibody Production by Committee on Methods of Producing Monoclonal Antibodies,Institute for Laboratory Animal Research,National Research Council Pdf

The American Anti-Vivisection Society (AAVS) petitioned the National Institutes of Health (NIH) in early 1997 to prohibit the use of an animal in the production of mAb (monoclonal antibodies). NIH responded late in 1997, asserting that continued use of the mouse method for producing mAb was scientifically required. In a second petition, in early 1998, AAVS did not accept the NIH response. NIH asked the National Research Council to form a committee to study this issue. The Committee on Methods of Producing Monoclonal Antibodies was composed of 11 experts with extensive experience in biomedical research, laboratory animal medicine, pain research, animal welfare, and patient advocacy. The committee was asked to determine whether there is a scientific necessity for producing mAb by the mouse method and, if so, to recommend ways to minimize any pain or distress that might be associated with the method. The committee was also to determine whether there are regulatory requirements for the mouse method and to summarize the current stage of development of tissue-culture methods.

Author : Vincent Ossipow,Nicolas Fischer
Publisher : Humana
Page : 0 pages
File Size : 44,6 Mb
Release : 2016-08-23
Category : Medical
ISBN : 1493963228

Get Book

Monoclonal Antibodies by Vincent Ossipow,Nicolas Fischer Pdf

Monoclonal Antibodies: Methods and Protocols, Second Edition expands upon the previous edition with current, detailed modern approaches to isolate and characterize monoclonal antibodies against carefully selected epitopes. This edition includes new chapters covering the key steps to generate high quality monoclonals via different methods, from antigen generation to epitope mapping and quality control of the purified IgG. Chapters are divided into four parts corresponding to four distinct objectives. Part I covers monoclonal antibody generation, Part II deals with monoclonal antibody expression and purification, Part III presents methods for monoclonal antibody characterization and modification, and Part IV describes selected applications of monoclonal antibodies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Monoclonal Antibodies: Methods and Protocols, Second Edition provides crucial initial steps of monoclonal antibody generation and characterization with state-of-the art protocols.

Author : Kenneth C. McCullough,Raymond E. Spier
Publisher : Cambridge University Press
Page : 401 pages
File Size : 53,9 Mb
Release : 1990-04-19
Category : Medical
ISBN : 9780521258906

Get Book

Monoclonal Antibodies in Biotechnology by Kenneth C. McCullough,Raymond E. Spier Pdf

Monoclonal antibodies are one of the most exciting developments in biotechnology in recent years; this book provides a comprehensive description of principles, methodologies and applications of this powerful technology to modern science and industry.

Author : A.M. Campbell
Publisher : Elsevier
Page : 426 pages
File Size : 54,9 Mb
Release : 1991-12-12
Category : Medical
ISBN : 008088735X

Get Book

Monoclonal Antibody and Immunosensor Technology by A.M. Campbell Pdf

This highly practical book, and successor to Volume 13 in the Laboratory Techniques series, explores further and provides more comprehensive, autoritative information on the production of Mabs. Much new and illuminating material has been included covering the concepts behind the application of recombinant DNA technology and biosensor technology to monoclonal antibodies, and all the human Mab technology facilitated by PCR of antibody genes. Also included in this latest volume is a section focussing on other methods of obtaining B cell clones such as short-term culture and oncogene transformation and an interesting section on Mab patents.

Author : Herve Bazin
Publisher : CRC Press
Page : 786 pages
File Size : 55,5 Mb
Release : 2017-11-22
Category : Medical
ISBN : 9781351358286

Get Book

Rat Hybridomas and Rat Monoclonal Antibodies (1990) by Herve Bazin Pdf

The purpose of this book is to provide information which supports the fact that rat hybridomas are no more difficult to develop than mouse hybridomas. This is the first book devoted to the development of rat hybridomas. It includes theories, step-by-step techniques, ingredients and apparatus. The focus of this work is on the antibody repertoire, the unique biological properties of rat immunoglobulins, the one-step purification procedure by immunoaffinity chromatography, the absence of C-type particles, and the easy production of large amounts of ascitic fluid containing rat MAb. This rare publication is an absolute must for all scientists using MAbs and those interested in the fields of immunology, biotechnology, and biochemistry.