Multimodal And Nanoscale Optical Microscopy

Author : P. Bianchini,A. Diaspro,C.J.R. Sheppard
Publisher : IOS Press
Page : 176 pages
File Size : 50,6 Mb
Release : 2023-09-11
Category : Science
ISBN : 9781643684154

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Multimodal and Nanoscale Optical Microscopy by P. Bianchini,A. Diaspro,C.J.R. Sheppard Pdf

Optical microscopy is developing into nanoscopy and multimodal microscopy the better to decipher the functioning mechanisms in living systems, and investigating biological specimens at molecular level using fluorescence as a mechanism of contrast. Results have demonstrated the potential to provide information at the Angstrom level. Other optical methods now offer more in terms of spatial and temporal resolution, making it possible to study the delicate and complex relationship between structure and function in cells. Modern optical microscopes also use the decisive advantage provided by artificial intelligence algorithms. All in all, a rapidly changing field with an increasing number of questions to be answered. This book contains the lectures presented as Course 210 of the prestigious International School of Physics Enrico Fermi, Multimodal and Nanoscale Optical Microscopy, held in Varenna, Italy from 11 to 16 July 2021. Topics covered include fluorescence; linear and nonlinear microscopy; label-free with Mueller matrix and Brillouin microscopy; F-methods such as FRAP, FLIM, FRET and FCS; super-resolution, phototoxicity and photodamage; optical and magnetic trapping; image formation; and bioimage analysis, among others. The book also includes selected contributions of a number of young researchers. Packed with novel ideas, new instruments, challenging theoretical approaches and amazing applications, the book will be of interest to all those working in the field of multimodal and nanoscale optical microscopy.

Author : P. Bianchini,A. Diaspro,C. J. Sheppard,M. Bouzin
Publisher : Unknown
Page : 0 pages
File Size : 50,5 Mb
Release : 2023
Category : Science
ISBN : 8874381352

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«Multimodal and nanoscale optical microscopy». Int. School of Physics Enrico Fermi Course 210 by P. Bianchini,A. Diaspro,C. J. Sheppard,M. Bouzin Pdf

Author : Alberto Diaspro
Publisher : CRC Press
Page : 450 pages
File Size : 52,8 Mb
Release : 2010-04-26
Category : Science
ISBN : 9781420078893

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Nanoscopy and Multidimensional Optical Fluorescence Microscopy by Alberto Diaspro Pdf

"Alberto Diaspro has been choreographing light's dance for over 20 years, and in Nanoscopy and Multidimensional Optical Fluorescence Microscopy, he has assembled a diverse group of experts to explain the methods they use to coax light to reveal biology's secrets."- From the Foreword by Daniel Evanko, editor, Nature Methods Nanoscopy and Multidimens

Author : Alberto Diaspro
Publisher : Springer
Page : 217 pages
File Size : 53,5 Mb
Release : 2022-04-20
Category : Science
ISBN : 3030944719

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Expedition into the Nanoworld by Alberto Diaspro Pdf

The story of microscopy over the years is one of wonder, revelation, and even love. What better words could there be to describe the amazing things that we have been able to see, learn and accomplish thanks to the progress made in this field? A love story between a pieace of glass and the rainbow with an original soundtrack mad of poetry and music. From Galilei’s initial foray into basic optical microscopy, including the Camillo Golgi and Giuliano Toraldo di Francia lessons, to such later developments as time-resolved microscopy, multi-photon microscopy and three-dimensional microscopy to innovations such as optical nanoscopy, bioimaging and super resolution imaging, the book seeks to take the reader, be they scientist or layperson, on a journey through the evolution of the microscope and its many uses, including in the field of medicine. The author uses visible light as a through-line to unite the various chapters, as well as using fluorescence as a touchpoint from which to map the changes in the science, a significant choice, as it, along with label-free approaches and the addition of artificial intelligence, form the natural environment for development of the modern multi-messenger microscope towards bioimaging at the nanoscale.

Author : Anatoly V. Zayats,David Richards
Publisher : Artech House
Page : 379 pages
File Size : 45,9 Mb
Release : 2009
Category : Science
ISBN : 9781596932845

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Nano-optics and Near-field Optical Microscopy by Anatoly V. Zayats,David Richards Pdf

"This groundbreaking book focuses on near-field microscopy which has opened up optical processes at the nanoscale for direct inspection. Further, it explores the emerging area of nano-optics which promises to make possible optical microscopy with true nanometer resolution. This frontline resource helps you achieve high resolution optical imaging of biological species and functional materials. You also find guidance in the imaging of optical device operation and new nanophotonics functionalities"--EBL.

Author : Philip Tinnefeld,Christian Eggeling,Stefan W. Hell
Publisher : Springer
Page : 340 pages
File Size : 41,6 Mb
Release : 2015-02-07
Category : Science
ISBN : 9783662455470

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Far-Field Optical Nanoscopy by Philip Tinnefeld,Christian Eggeling,Stefan W. Hell Pdf

This book describes developments in the field of super-resolution fluorescence microscopy or nanoscopy. In 11 chapters, distinguished scientists and leaders in their respective fields describe different nanoscopy approaches, various labeling technologies, and concrete applications. The topics covered include the principles and applications of the most popular nanoscopy techniques STED and (f)PALM/STORM, along with advances brought about by fluorescent proteins and organic dyes optimized for fluorescence nanoscopy. Furthermore, the photophysics of fluorescent labels is addressed, specifically for improving their photoswitching capabilities. Important applications are also discussed, such as the tracking and counting of molecules to determine acting forces in cells, and quantitative cellular imaging, respectively, as well as the mapping of chemical reaction centers at the nano-scale. The 2014 Chemistry Nobel Prize® was awarded for the ground-breaking developments of super-resolved fluorescence microscopy. In this book, which was co-edited by one of the prize winners, readers will find the most recent developments in this field.

Author : Alberto Diaspro
Publisher : Springer Science & Business Media
Page : 252 pages
File Size : 54,7 Mb
Release : 2010-11-25
Category : Science
ISBN : 9783642151750

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Optical Fluorescence Microscopy by Alberto Diaspro Pdf

In the last decade, fluorescence microscopy has evolved from a classical “retrospective” microscopy approach into an advanced imaging technique that allows the observation of cellular activities in living cells with increased resolution and dimensions. A bright new future has arrived as the nano era has placed a whole new array of tools in the hands of biophysicists who are keen to go deeper into the intricacies of how biological systems work. Following an introduction to the complex world of optical microscopy, this book covers topics such as the concept of white confocal, nonlinear optical microscopy, fluctuation spectroscopies, site-specific labeling of proteins in living cells, imaging molecular physiology using nanosensors, measuring molecular dynamics, muscle braking and stem cell differentiation.

Author : Ping-chin Cheng
Publisher : World Scientific
Page : 344 pages
File Size : 49,9 Mb
Release : 1999
Category : Science
ISBN : 9810239920

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Focus on Multidimensional Microscopy by Ping-chin Cheng Pdf

This book covers various aspects of modern microscopy, with emphasis on multidimensional (three-dimensional and higher) and multimodality microscopy. The topics discussed include multiphoton fluorescent microscopy, confocal microscopy, x-ray microscopy and microtomography, electron microscopy, probe microscopy and multidimensional image processing for microscopy. In addition, there are chapters demonstrating typical microscopical applications, both biological and material.

Author : Hanry Yu,Ping-chin Cheng,Pao-chun Lin,Fu-jen Kao
Publisher : World Scientific
Page : 317 pages
File Size : 48,8 Mb
Release : 2006-05-08
Category : Technology & Engineering
ISBN : 9789814479127

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Multi-modality Microscopy by Hanry Yu,Ping-chin Cheng,Pao-chun Lin,Fu-jen Kao Pdf

This book covers important aspects of modern optical microscopy and image restoration technologies. Instead of pure optical treatment, the book is delivered with the consideration of the scientists who utilize optical microscopy in their daily research. However, enough details are provided in basic imaging principles, optics and instrumentation in microscopy, spherical aberrations, deconvolution and image restoration. A number of microscopic technologies such as polarization, confocal and multi-photon microscopy are highlighted with their applications in biological and materials sciences/engineering.

Author : Peng Xi
Publisher : CRC Press
Page : 268 pages
File Size : 42,8 Mb
Release : 2014-09-19
Category : Science
ISBN : 9781466586307

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Optical Nanoscopy and Novel Microscopy Techniques by Peng Xi Pdf

Microscopy is at the forefront of multidisciplinary research. It was developed by physicists, made specific by chemists, and applied by biologists and doctors to better understand how the human body works. For this very reason, the field has been revolutionized in past decades.The objective of Optical Nanoscopy and Novel Microscopy Techniques is to

Author : A. Di Giuseppe
Publisher : IOS Press
Page : 567 pages
File Size : 43,9 Mb
Release : 2013-10-21
Category : Science
ISBN : 9781614993261

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Metrology and Physical Constants by A. Di Giuseppe Pdf

The reliability and accuracy of systems of measurement continue to advance. We are about to enter a period of the most stable measurement system we can imagine with the anticipated new definitions of the SI units of measurement; a direct link between fundamental physics and metrology which will eliminate the current definition of the kilogram, until now based upon an artifact. This book presents selected papers from Course 185 of the Enrico Fermi International School of Physics, held in Varenna, Italy, in July 2012 and jointly organized with the Bureau International des Poids et Mesures (BIPM). The papers delivered at the school covered some of the most advanced topics in the discipline of metrology, including nano-technologies; quantum information and quantum devices; biology and medicine; food; surface quality; ionising radiation for health, environment, art and archaeology; and climate. The continuous and striking advances in basic research concerning atomic frequency standards operating both in the visible range and at microwave levels and the applications to satellite systems are also considered, in the framework of a historical review of the international organization of metrology, as are the problems inherent in uncertainty statements and definitions. This book will be of interest to all those whose work involves scientific measurement at the highest levels of accuracy.

Author : Jean-pierre Fillard
Publisher : World Scientific
Page : 460 pages
File Size : 40,8 Mb
Release : 1996-09-20
Category : Science
ISBN : 9789814520140

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Near Field Optics And Nanoscopy by Jean-pierre Fillard Pdf

This book contains the most recent information on optical nanoscopy. Far-Field and Near-Field properties on e.m. waves are presented which illustrate how optical images can be obtained from sub-micron objects. Scanning Probe techniques and computer processing are covered here. An explanation is given on how propagating photons or evanescent waves can behave over distances shorter than the wavelength, taking into account the presence of small objects. Quantum tunneling of photons is explained comparatively with the electron mechanism. Technical details are given on photon tunneling microscopes. Typical results already obtained with these techniques are also described.

Author : Jordan A. Hachtel
Publisher : Springer
Page : 129 pages
File Size : 43,6 Mb
Release : 2017-12-09
Category : Science
ISBN : 9783319702599

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The Nanoscale Optical Properties of Complex Nanostructures by Jordan A. Hachtel Pdf

This book presents studies of complex nanostructures with unique optical responses from both theoretical and experimental perspectives. The theory approaches the optical response of a complex structure from both quantum-mechanical and semiclassical frameworks, and is used to understand experimental results at a fundamental level as well as to form a quantitative model to allow the design of custom nanostructures. The experiments utilize scanning transmission electron microscopy and its associated analytical spectroscopies to observe nanoscale optical effects, such as surface plasmon resonances, with nanometer-scale spatial resolution. Furthermore, there is a focus in the dissertation on the combination of distinct techniques to study the difficult-to-access aspects of the nanoscale response of complex nanostructures: the combination of complementary spectroscopies, the combination of electron microscopy and photonics, and the combination of experiment and theory. Overall, the work demonstrates the importance of observing nanoscale optical phenomena in complex structures, and observing them directly at the nanoscale.

Author : J. Hastings,C. Pellegrini,A. Marinelli
Publisher : IOS Press
Page : 272 pages
File Size : 49,5 Mb
Release : 2020-12-18
Category : Science
ISBN : 9781643681337

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Physics of and Science with X-Ray Free-Electron Lasers by J. Hastings,C. Pellegrini,A. Marinelli Pdf

Many X-Ray Free-Electron Lasers (X-FELs) have been designed, built and commissioned since the first lasing of the Linac Coherent Light Source in the hard and soft X-ray regions, and great progress has been made in improving their performance and extending their capabilities. Meanwhile, experimental techniques to exploit the unique properties of X-FELs to explore atomic and molecular systems of interest to physics, chemistry, biology and the material sciences have also been developed. As a result, our knowledge of atomic and molecular science has been greatly extended. Nevertheless, there is still much to be accomplished, and the potential for discovery with X-FELs is still largely unexplored. The next generation of scientists will need to be well versed in both particle beams/FEL physics and X-ray photon science. This book presents material from the Enrico Fermi summer school: Physics of and Science with X-Ray Free-Electron Lasers, held at the Enrico Fermi International School of Physics in Varenna, Italy, from 26 June - 1 July 2017. The lectures presented at the school were aimed at introducing graduate students and young scientists to this fast growing and exciting scientific area, and subjects covered include basic accelerator and FEL physics, as well as an introduction to the main research topics in X-FEL-based biology, atomic molecular optical science, material sciences, high-energy density physics and chemistry. Bridging the gap between accelerator/FEL physicists and scientists from other disciplines, the book will be of interest to all those working in the field.

Author : John Girkin
Publisher : CRC Press
Page : 247 pages
File Size : 42,5 Mb
Release : 2019-06-14
Category : Science
ISBN : 9781351630351

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A Practical Guide to Optical Microscopy by John Girkin Pdf

Choice Recommended Title, March 2020 Optical microscopy is used in a vast range of applications ranging from materials engineering to in vivo observations and clinical diagnosis, and thanks to the latest advances in technology, there has been a rapid growth in the number of methods available. This book is aimed at providing users with a practical guide to help them select, and then use, the most suitable method for their application. It explores the principles behind the different forms of optical microscopy, without the use of complex maths, to provide an understanding to help the reader utilise a specific method and then interpret the results. Detailed physics is provided in boxed sections, which can be bypassed by the non-specialist. It is an invaluable tool for use within research groups and laboratories in the life and physical sciences, acting as a first source for practical information to guide less experienced users (or those new to a particular methodology) on the range of techniques available. Features: The first book to cover all current optical microscopy methods for practical applications Written to be understood by a non-optical expert with inserts to provide the physical science background Brings together conventional widefield and confocal microscopy, with advanced non-linear and super resolution methods, in one book To learn more about the author please visit here.