Optical Nanoscopy And Novel Microscopy Techniques

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Optical Nanoscopy and Novel Microscopy Techniques

Peng Xi
Optical Nanoscopy and Novel Microscopy Techniques Book PDF

Author : Peng Xi
Publisher : CRC Press
Page : 278 pages
File Size : 50,5 Mb
Release : 2014-09-19
Category : Science
ISBN : 9781466586291

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Optical Nanoscopy and Novel Microscopy Techniques by Peng Xi Pdf

Microscopy is at the forefront of multidisciplinary research. It was developed by physicists, made specific by chemists, and applied by biologists and doctors to better understand how the human body works. For this very reason, the field has been revolutionized in past decades. The objective of Optical Nanoscopy and Novel Microscopy Techniques is to choose some of those revolutionary ideas and serve a general audience from broad disciplines to achieve a fundamental understanding of these technologies and to better apply them in their daily research. The book begins with coverage of super-resolution optical microscopy, which discusses targeted modulation such as STED and SIM or localization methods such as PALM. It then discusses novel development of fluorescent probes, such as organic small-molecule probes, fluorescent proteins, and inorganic labels such as quantum dots. Finally, it describes advanced optical microscopy, such as fluorescence lifetime imaging, fiber optic microscopy, scanning ion conductance microscopy, and the joining of optics and acoustics—photoacoustic microscopy. Following each chapter, a detailed list of references is provided. Problems at the end of each chapter are also included.

Nanoscopy and Multidimensional Optical Fluorescence Microscopy

Alberto Diaspro
Nanoscopy and Multidimensional Optical Fluorescence Microscopy Book PDF

Author : Alberto Diaspro
Publisher : CRC Press
Page : 450 pages
File Size : 54,8 Mb
Release : 2010-04-26
Category : Science
ISBN : 9781420078893

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Nanoscopy and Multidimensional Optical Fluorescence Microscopy by Alberto Diaspro Pdf

"Alberto Diaspro has been choreographing light's dance for over 20 years, and in Nanoscopy and Multidimensional Optical Fluorescence Microscopy, he has assembled a diverse group of experts to explain the methods they use to coax light to reveal biology's secrets."- From the Foreword by Daniel Evanko, editor, Nature Methods Nanoscopy and Multidimens

Biomedical Optical Phase Microscopy and Nanoscopy

Natan T. Shaked,Zeev Zalevsky,Lisa L Satterwhite
Biomedical Optical Phase Microscopy and Nanoscopy Book PDF

Author : Natan T. Shaked,Zeev Zalevsky,Lisa L Satterwhite
Publisher : Academic Press
Page : 428 pages
File Size : 41,9 Mb
Release : 2012-11-05
Category : Medical
ISBN : 9780124158719

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Biomedical Optical Phase Microscopy and Nanoscopy by Natan T. Shaked,Zeev Zalevsky,Lisa L Satterwhite Pdf

Written by leading optical phase microscopy experts, this book is a comprehensive reference to phase microscopy and nanoscopy techniques for biomedical applications, including differential interference contrast (DIC) microscopy, phase contrast microscopy, digital holographic microscopy, optical coherence tomography, tomographic phase microscopy, spectral-domain phase detection, and nanoparticle usage for phase nanoscopy The Editors show biomedical and optical engineers how to use phase microscopy for visualizing unstained specimens, and support the theoretical coverage with applied content and examples on designing systems and interpreting results in bio- and nanoscience applications. Provides a comprehensive overview of the principles and techniques of optical phase microscopy and nanoscopy with biomedical applications. Tips/advice on building systems and working with advanced imaging biomedical techniques, including interpretation of phase images, and techniques for quantitative analysis based on phase microscopy. Interdisciplinary approach that combines optical engineering, nanotechnology, biology and medical aspects of this topic. Each chapter includes practical implementations and worked examples.

Far-Field Optical Nanoscopy

Philip Tinnefeld,Christian Eggeling,Stefan W. Hell
Far Field Optical Nanoscopy Book PDF

Author : Philip Tinnefeld,Christian Eggeling,Stefan W. Hell
Publisher : Springer
Page : 335 pages
File Size : 40,7 Mb
Release : 2015-02-07
Category : Science
ISBN : 9783662455470

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Far-Field Optical Nanoscopy by Philip Tinnefeld,Christian Eggeling,Stefan W. Hell Pdf

This book describes developments in the field of super-resolution fluorescence microscopy or nanoscopy. In 11 chapters, distinguished scientists and leaders in their respective fields describe different nanoscopy approaches, various labeling technologies, and concrete applications. The topics covered include the principles and applications of the most popular nanoscopy techniques STED and (f)PALM/STORM, along with advances brought about by fluorescent proteins and organic dyes optimized for fluorescence nanoscopy. Furthermore, the photophysics of fluorescent labels is addressed, specifically for improving their photoswitching capabilities. Important applications are also discussed, such as the tracking and counting of molecules to determine acting forces in cells, and quantitative cellular imaging, respectively, as well as the mapping of chemical reaction centers at the nano-scale. The 2014 Chemistry Nobel Prize® was awarded for the ground-breaking developments of super-resolved fluorescence microscopy. In this book, which was co-edited by one of the prize winners, readers will find the most recent developments in this field.

Near Field Optics And Nanoscopy

Jean-pierre Fillard
Near Field Optics And Nanoscopy Book PDF

Author : Jean-pierre Fillard
Publisher : World Scientific
Page : 460 pages
File Size : 42,8 Mb
Release : 1996-09-20
Category : Science
ISBN : 9789814520140

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Near Field Optics And Nanoscopy by Jean-pierre Fillard Pdf

This book contains the most recent information on optical nanoscopy. Far-Field and Near-Field properties on e.m. waves are presented which illustrate how optical images can be obtained from sub-micron objects. Scanning Probe techniques and computer processing are covered here. An explanation is given on how propagating photons or evanescent waves can behave over distances shorter than the wavelength, taking into account the presence of small objects. Quantum tunneling of photons is explained comparatively with the electron mechanism. Technical details are given on photon tunneling microscopes. Typical results already obtained with these techniques are also described.

Super Resolution Optical Imaging and Microscopy

Junle Qu,Zhigang Yang
Super Resolution Optical Imaging and Microscopy Book PDF

Author : Junle Qu,Zhigang Yang
Publisher : John Wiley & Sons
Page : 261 pages
File Size : 54,9 Mb
Release : 2023-12-15
Category : Science
ISBN : 9783527835522

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Super Resolution Optical Imaging and Microscopy by Junle Qu,Zhigang Yang Pdf

Super Resolution Optical Imaging and Microscopy Extremely comprehensive resource containing cutting-edge and practical knowledge of super-resolution optical imaging This book covers both the basic principles and specific technical details of super-resolution microscopy techniques. It covers the criteria to choose different fluorophores for various SRM methods and critically assesses the nitty-gritty of associated problems that are often encountered in practical applications. A progressive guide to designing the next generation of advanced fluorophores to meet the goal of advanced SR imaging studies is also put forward. Written by two well-qualified authors, the book contains exclusive content to enhance readers’ understanding on innovation of newer SRM technologies. Sample topics covered in the book include: Optical techniques, fluorescent probe design, and algorithm development Recent highlight and breakthroughs in biology using SRM methods The overall success of SRM in biological inventions The future direction and scope of the field This book is an invaluable resource for chemists and researchers/scientists involved in designing newer fluorescent materials for SRM studies. It can also assist biologists engaged in advanced biological studies using SRM by guiding them through sample preparation, image processing, and precautions to be taken in practical imaging studies.

Handbook of Nanoscopy, 2 Volume Set

Gustaaf van Tendeloo,Dirk van Dyck,Stephen J. Pennycook
Handbook of Nanoscopy 2 Volume Set Book PDF

Author : Gustaaf van Tendeloo,Dirk van Dyck,Stephen J. Pennycook
Publisher : John Wiley & Sons
Page : 1484 pages
File Size : 42,6 Mb
Release : 2012-05-21
Category : Technology & Engineering
ISBN : 9783527317066

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Handbook of Nanoscopy, 2 Volume Set by Gustaaf van Tendeloo,Dirk van Dyck,Stephen J. Pennycook Pdf

This completely revised successor to the Handbook of Microscopy supplies in-depth coverage of all imaging technologies from the optical to the electron and scanning techniques. Adopting a twofold approach, the book firstly presents the various technologies as such, before going on to cover the materials class by class, analyzing how the different imaging methods can be successfully applied. It covers the latest developments in techniques, such as in-situ TEM, 3D imaging in TEM and SEM, as well as a broad range of material types, including metals, alloys, ceramics, polymers, semiconductors, minerals, quasicrystals, amorphous solids, among others. The volumes are divided between methods and applications, making this both a reliable reference and handbook for chemists, physicists, biologists, materials scientists and engineers, as well as graduate students and their lecturers.

New Techniques of Optical Microscopy and Microspectroscopy

Richard J. Cherry
New Techniques of Optical Microscopy and Microspectroscopy Book PDF

Author : Richard J. Cherry
Publisher : Unknown
Page : 279 pages
File Size : 45,8 Mb
Release : 1991
Category : Microscope and microscopy
ISBN : 0333491084

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New Techniques of Optical Microscopy and Microspectroscopy by Richard J. Cherry Pdf

The advent of various techniques such as confocal microscopy and the availability of extremely sensitive digital imaging devices are revolutionizing the field of optical microscopy. This book intends to bring together the various advances in order to provide an account of what can be achieved with modern optical microscopic methods.

Introduction to Optical Microscopy

Jerome Mertz
Introduction to Optical Microscopy Book PDF

Author : Jerome Mertz
Publisher : Cambridge University Press
Page : 475 pages
File Size : 51,7 Mb
Release : 2019-08
Category : Medical
ISBN : 9781108428309

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Introduction to Optical Microscopy by Jerome Mertz Pdf

Presents a fully updated, self-contained textbook covering the core theory and practice of both classical and modern optical microscopy techniques.

A Practical Guide to Optical Microscopy

John Girkin
A Practical Guide to Optical Microscopy Book PDF

Author : John Girkin
Publisher : CRC Press
Page : 247 pages
File Size : 48,9 Mb
Release : 2019-06-14
Category : Science
ISBN : 9781351630351

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A Practical Guide to Optical Microscopy by John Girkin Pdf

Choice Recommended Title, March 2020 Optical microscopy is used in a vast range of applications ranging from materials engineering to in vivo observations and clinical diagnosis, and thanks to the latest advances in technology, there has been a rapid growth in the number of methods available. This book is aimed at providing users with a practical guide to help them select, and then use, the most suitable method for their application. It explores the principles behind the different forms of optical microscopy, without the use of complex maths, to provide an understanding to help the reader utilise a specific method and then interpret the results. Detailed physics is provided in boxed sections, which can be bypassed by the non-specialist. It is an invaluable tool for use within research groups and laboratories in the life and physical sciences, acting as a first source for practical information to guide less experienced users (or those new to a particular methodology) on the range of techniques available. Features: The first book to cover all current optical microscopy methods for practical applications Written to be understood by a non-optical expert with inserts to provide the physical science background Brings together conventional widefield and confocal microscopy, with advanced non-linear and super resolution methods, in one book To learn more about the author please visit here.

Optical Imaging and Microscopy

Fu-Jen Kao,Peter Török
Optical Imaging and Microscopy Book PDF

Author : Fu-Jen Kao,Peter Török
Publisher : Unknown
Page : 420 pages
File Size : 49,6 Mb
Release : 2003
Category : Imaging systems
ISBN : 366214381X

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Optical Imaging and Microscopy by Fu-Jen Kao,Peter Török Pdf

This text on contemporary optical systems is intended for optical researchers and engineers, graduate students and optical microscopists in the biological and biomedical sciences. It consists of three parts: The first discusses high-aperture optical systems, which form the backbone of optical microscopes. Here particular attention is paid to optical data storage. The second part is on the use of non-linear optical techniques, including non-linear optical excitation (second and third harmonic generation and two-photon microscopy) and non-linear spectroscopy (CARS). The final part of the book presents miscellaneous techniques that are either novel or well known but finding new applications. An example of the latter is adaptive optics, which is a well-established method, for example, in astronomy. However, its application in eye imaging in combination with high power pulsed lasers, opens a novel approach to eye surgery.

Super-Resolution Imaging in Biomedicine

Alberto Diaspro,Marc A. M. J. van Zandvoort
Super Resolution Imaging in Biomedicine Book PDF

Author : Alberto Diaspro,Marc A. M. J. van Zandvoort
Publisher : CRC Press
Page : 525 pages
File Size : 47,5 Mb
Release : 2016-11-03
Category : Science
ISBN : 9781315355566

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Super-Resolution Imaging in Biomedicine by Alberto Diaspro,Marc A. M. J. van Zandvoort Pdf

This book encompasses the full breadth of the super-resolution imaging field, representing modern techniques that exceed the traditional diffraction limit, thereby opening up new applications in biomedicine. It shows readers how to use the new tools to increase resolution in sub-nanometer-scale images of living cells and tissue, which leads to new information about molecules, pathways and dynamics. The book highlights the advantages and disadvantages of the techniques, and gives state-of-the-art examples of applications using microscopes currently available on the market. It covers key techniques such as stimulated emission depletion (STED), structured illumination microscopy (SSIM), photoactivated localization microscopy (PALM), and stochastic optical reconstruction microscopy (STORM). It will be a useful reference for biomedical researchers who want to work with super-resolution imaging, learn the proper technique for their application, and simultaneously obtain a solid footing in other techniques.

Biomedical Optical Imaging

James G. Fujimoto,Daniel Farkas
Biomedical Optical Imaging Book PDF

Author : James G. Fujimoto,Daniel Farkas
Publisher : Oxford University Press
Page : 440 pages
File Size : 41,8 Mb
Release : 2009-04-22
Category : Science
ISBN : 0199722293

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Biomedical Optical Imaging by James G. Fujimoto,Daniel Farkas Pdf

Biomedical optical imaging is a rapidly emerging research area with widespread fundamental research and clinical applications. This book gives an overview of biomedical optical imaging with contributions from leading international research groups who have pioneered many of these techniques and applications. A unique research field spanning the microscopic to the macroscopic, biomedical optical imaging allows both structural and functional imaging. Techniques such as confocal and multiphoton microscopy provide cellular level resolution imaging in biological systems. The integration of this technology with exogenous chromophores can selectively enhance contrast for molecular targets as well as supply functional information on processes such as nerve transduction. Novel techniques integrate microscopy with state-of-the-art optics technology, and these include spectral imaging, two photon fluorescence correlation, nonlinear nanoscopy; optical coherence tomography techniques allow functional, dynamic, nanoscale, and cross-sectional visualization. Moving to the macroscopic scale, spectroscopic assessment and imaging methods such as fluorescence and light scattering can provide diagnostics of tissue pathology including neoplastic changes. Techniques using light diffusion and photon migration are a means to explore processes which occur deep inside biological tissues and organs. The integration of these techniques with exogenous probes enables molecular specific sensitivity.

Super-Resolution Microscopy

Udo J. Birk
Super Resolution Microscopy Book PDF

Author : Udo J. Birk
Publisher : John Wiley & Sons
Page : 408 pages
File Size : 41,9 Mb
Release : 2017-09-12
Category : Science
ISBN : 9783527802081

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Super-Resolution Microscopy by Udo J. Birk Pdf

This unique book on super-resolution microscopy techniques presents comparative, in-depth analyses of the strengths and weaknesses of the individual approaches. It was written for non-experts who need to understand the principles of super-resolution or who wish to use recently commercialized instruments as well as for professionals who plan to realize novel microscopic devices. Explaining the practical requirements in terms of hardware, software and sample preparation, the book offers a wealth of hands-on tips and practical tricks to get a setup running, provides invaluable help and support for successful data acquisition and specific advice in the context of data analysis and visualization. Furthermore, it addresses a wide array of transdisciplinary fields of applications. The author begins by outlining the joint efforts that have led to achieving super-resolution microscopy combining advances in single-molecule photo-physics, fluorophore design and fluorescent labeling, instrument design and software development. The following chapters depict and compare current main standard techniques such as structured illumination microscopy, single-molecule localization, stimulated emission depletion microscopy and multi-scale imaging including light-sheet and expansion microscopy. For each individual approach the experimental setups are introduced, the imaging protocols are provided and the various applications illustrated. The book concludes with a discussion of future challenges addressing issues of routine applications and further commercialization of the available methods. Guiding users in how to make choices for the design of their own experiments from scratch to promising application, this one-stop resource is intended for researchers in the applied sciences, from chemistry to biology and medicine to physics and engineering.

Multimodal and Nanoscale Optical Microscopy

P. Bianchini,A. Diaspro,C.J.R. Sheppard
Multimodal and Nanoscale Optical Microscopy Book PDF

Author : P. Bianchini,A. Diaspro,C.J.R. Sheppard
Publisher : IOS Press
Page : 176 pages
File Size : 44,9 Mb
Release : 2023-09-11
Category : Science
ISBN : 9781643684154

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Multimodal and Nanoscale Optical Microscopy by P. Bianchini,A. Diaspro,C.J.R. Sheppard Pdf

Optical microscopy is developing into nanoscopy and multimodal microscopy the better to decipher the functioning mechanisms in living systems, and investigating biological specimens at molecular level using fluorescence as a mechanism of contrast. Results have demonstrated the potential to provide information at the Angstrom level. Other optical methods now offer more in terms of spatial and temporal resolution, making it possible to study the delicate and complex relationship between structure and function in cells. Modern optical microscopes also use the decisive advantage provided by artificial intelligence algorithms. All in all, a rapidly changing field with an increasing number of questions to be answered. This book contains the lectures presented as Course 210 of the prestigious International School of Physics Enrico Fermi, Multimodal and Nanoscale Optical Microscopy, held in Varenna, Italy from 11 to 16 July 2021. Topics covered include fluorescence; linear and nonlinear microscopy; label-free with Mueller matrix and Brillouin microscopy; F-methods such as FRAP, FLIM, FRET and FCS; super-resolution, phototoxicity and photodamage; optical and magnetic trapping; image formation; and bioimage analysis, among others. The book also includes selected contributions of a number of young researchers. Packed with novel ideas, new instruments, challenging theoretical approaches and amazing applications, the book will be of interest to all those working in the field of multimodal and nanoscale optical microscopy.