Oligonucleotides And Analogues

Author : Fritz Eckstein
Publisher : Oxford University Press, USA
Page : 346 pages
File Size : 42,5 Mb
Release : 1991
Category : Language Arts & Disciplines
ISBN : UOM:39015058985592

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Oligonucleotides and Analogues by Fritz Eckstein Pdf

Interest in oligonucleotide synthesis has expanded dramatically since the publication of M. Gait's book in this series in 1984. The process has been automated, new reagents have been developed, and the range of uses for the compounds produced has increased and continues to grow. This volume provides practical guidance on oligonucleotide synthesis and methods for introducing modifications into these molecules. State- of-the-art techniques for automated synthesis are covered in two chapters on the production of oligodeoxynucleotides and oligoribonucleotides. Synthesis of modified oligodeoxynucleotides is also detailed, including modification of the phosphate backbone to produce phosphorothioates, phosphorodithiolates and methyl phosphonates, all of which are of considerable importance due to thier potential therapeutic applications. Other chapters decsribe production of sugar-modified ilgodeoxynucleotides and the attachment of various reported groups; these techniques are useful to those interested in non-radioactive probes for hybridization and for the study of DNA-DNA and DNA protein interactions. This book will be of interest to academic and industrial researchers, enabling both chemists and non-chemists to synthesize oligonucleotides and analogues for a wide variety of experimental purposes.

Author : Sudhir Agrawal
Publisher : Springer Science & Business Media
Page : 503 pages
File Size : 41,8 Mb
Release : 1993-08-31
Category : Science
ISBN : 9780896032811

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Protocols for Oligonucleotides and Analogs by Sudhir Agrawal Pdf

When first conceived, not only was the aim of Protocols for Oligo nucleotides and Analogs to provide wide coverage of the ohgonuc- otide chemistry field for readers who are well versed within the field, but also to give investigators just entering into the field a new perspective. The very first book on this topic was edited and published by Michael Gait in 1984, in whose laboratory I encountered the newer aspects of oligonucleotide chemistry. Since then, oligonucleotide research has developed to such an extent that its uses extend far beyond basic studies, and now find wide application throughout clinical science as well. Until recently, the major application of oligonucleotides has been in the area of DNA-based diagnostic and "antisense oligonucleotid- based therapeutic approaches. However, oligonucleotides are now also being used as therapeutic agents and are thus frequently found in clinical trials in humans. Synthesis of unmodified oligonucleotides using automated synthe sizers has become a common practice in numerous laboratories. How ever, improvements on the existing techniques and the introduction of ever newer methods for oligonucleotide synthesis is constantly driving ahead in the leading research laboratories. And several new oligonucle otide analogs have been synthesized and studied for their individual prop erties in recent years. The present volume strives to bring the readers the most up-to-date information on the newest aspects of synthesis of oligo nucleotides and their analogs. A separate volume covers synthesis of oligonucleotide conjugates, along with most of the analytical techniques presently used for analysis of oligonucleotides.

Author : Fritz Eckstein
Publisher : Unknown
Page : 0 pages
File Size : 43,9 Mb
Release : 2024-07-02
Category : Oligonucleotides
ISBN : 1383048274

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Oligonucleotides and Analogues by Fritz Eckstein Pdf

Provides practical guidance on the synthesis and chemical modification of oligonucleotides. These molecules have a wide variety of experimental uses, including use as potential therapeutics and in non-radioactive probing for the study of DNA-DNA and DNA-protein interactions.

Author : Nicolay Ferrari,Rosanne Seguin
Publisher : John Wiley & Sons
Page : 563 pages
File Size : 51,6 Mb
Release : 2018-06-06
Category : Medical
ISBN : 9781119070306

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Oligonucleotide-Based Drugs and Therapeutics by Nicolay Ferrari,Rosanne Seguin Pdf

A comprehensive review of contemporary antisense oligonucleotides drugs and therapeutic principles, methods, applications, and research Oligonucleotide-based drugs, in particular antisense oligonucleotides, are part of a growing number of pharmaceutical and biotech programs progressing to treat a wide range of indications including cancer, cardiovascular, neurodegenerative, neuromuscular, and respiratory diseases, as well as other severe and rare diseases. Reviewing fundamentals and offering guidelines for drug discovery and development, this book is a practical guide covering all key aspects of this increasingly popular area of pharmacology and biotech and pharma research, from the basic science behind antisense oligonucleotides chemistry, toxicology, manufacturing, to safety assessments, the design of therapeutic protocols, to clinical experience. Antisense oligonucleotides are single strands of DNA or RNA that are complementary to a chosen sequence. While the idea of antisense oligonucleotides to target single genes dates back to the 1970's, most advances have taken place in recent years. The increasing number of antisense oligonucleotide programs in clinical development is a testament to the progress and understanding of pharmacologic, pharmacokinetic, and toxicologic properties as well as improvement in the delivery of oligonucleotides. This valuable book reviews the fundamentals of oligonucleotides, with a focus on antisense oligonucleotide drugs, and reports on the latest research underway worldwide. • Helps readers understand antisense molecules and their targets, biochemistry, and toxicity mechanisms, roles in disease, and applications for safety and therapeutics • Examines the principles, practices, and tools for scientists in both pre-clinical and clinical settings and how to apply them to antisense oligonucleotides • Provides guidelines for scientists in drug design and discovery to help improve efficiency, assessment, and the success of drug candidates • Includes interdisciplinary perspectives, from academia, industry, regulatory and from the fields of pharmacology, toxicology, biology, and medicinal chemistry Oligonucleotide-Based Drugs and Therapeutics belongs on the reference shelves of chemists, pharmaceutical scientists, chemical biologists, toxicologists and other scientists working in the pharmaceutical and biotechnology industries. It will also be a valuable resource for regulatory specialists and safety assessment professionals and an important reference for academic researchers and post-graduates interested in therapeutics, antisense therapy, and oligonucleotides.

Author : Derek J. Chadwick,Gail Cardew
Publisher : John Wiley & Sons
Page : 260 pages
File Size : 54,5 Mb
Release : 2008-04-30
Category : Science
ISBN : 9780470515402

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Oligonucleotides as Therapeutic Agents by Derek J. Chadwick,Gail Cardew Pdf

The use of oligonucleotides as therapeutic agents rests upon their ability to interfere, in a sequence-specific manner, with the fundamental machinery of protein synthesis either by binding to the mRNAs transcribed from a gene or by binding directly to a target gene. This approach can be used not only for inhibition of the synthesis of host proteins but also of those required by invading pathogens. Potential therapeutic applications are enormous, ranging over hypertension, cardiovascular disease, autoimmune disease, vital and other parasitic infections (especially HIV), and cancer. This book discusses the chemistry and pharmacokinetics of oligonucleotides and their analogues, and surveys the results of structure-activity studies and current clinical trials. It also critically reviews the problems with antisense therapy, such as the enzymatic destruction of oligonucleotides, the doses required for a therapeutic response, the difficulty in directing oligonucleotides to particular target tissues and cells, the need for parenteral administration, and doubts concerning the mechanism of action (especially problems associated with non-specific binding to proteins) and long-term effects.

Author : Satoshi Obika,Mitsuo Sekine
Publisher : Springer
Page : 284 pages
File Size : 52,8 Mb
Release : 2018-12-08
Category : Medical
ISBN : 9789811319129

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Synthesis of Therapeutic Oligonucleotides by Satoshi Obika,Mitsuo Sekine Pdf

This book presents the latest knowledge on a broad range of topics relating to the synthesis of natural and artificial oligonucleotides with therapeutic potential. Nucleic acid-based therapeutics are attracting much attention, and numerous therapeutic oligonucleotides, such as antisense oligonucleotides, siRNAs, splice-switching oligonucleotides, and nucleic acid aptamers, are being evaluated in clinical trials for the treatment of a variety of diseases. Synthesis of Therapeutic Oligonucleotides covers a broad range of topics in the field that are of high relevance to researchers, including the synthesis of natural and chemically modified oligonucleotides, the development of novel nucleic acid analogs, industrial scale synthesis and purification of oligonucleotides, and important aspects of chemistry, manufacturing, and controls (CMC). The aim is to provide new insights and inspire fresh ideas in nucleic acid chemistry that may ultimately lead to novel concepts and techniques and the discovery of more effective nucleic acid drugs. The book will be of high value for both established researchers in the field and students intending to specialize in nucleic acid chemistry research.

Author : Piet Herdewijn
Publisher : Springer Science & Business Media
Page : 436 pages
File Size : 52,8 Mb
Release : 2008-02-04
Category : Medical
ISBN : 9781592598236

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Oligonucleotide Synthesis by Piet Herdewijn Pdf

A collection of powerful new techniques for oligonucleotide synthesis and for the use of modified oligonucleotides in biotechnology. Among the protocol highlights are a novel two-step process that yields a high purity, less costly, DNA, the synthesis of phosphorothioates using new sulfur transfer agents, the synthesis of LNA, peptide conjugation methods to improve cellular delivery and cell-specific targeting, and triple helix formation. The applications include using molecular beacons to monitor the PCR amplification process, nuclease footprinting to study the sequence-selective binding of small molecules of DNA, nucleic acid libraries, and the use of small interference RNA (siRNA) as an inhibitor of gene expression.

Author : Stanley T. Crooke,Bernard Lebleu
Publisher : CRC Press
Page : 620 pages
File Size : 45,8 Mb
Release : 1993-05-27
Category : Medical
ISBN : 084934705X

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Antisense Research and Applications by Stanley T. Crooke,Bernard Lebleu Pdf

Antisense Research and Applications is a comprehensive review of oligonucleotide research covering molecular biological advances in this field, the current status of antisense drug research, and strategies for future research and therapeutic applications. In bringing together the latest research from an array of authoritative scientists, Antisense Research and Applications provides an integrated conceptual basis for considering oligonucleotide therapeutics. Topics covered in the 32 chapters of this book include nucleic acid structure and function, antisense RNA, medicinal chemistry of oligonucleotides, analogs, pharmacokinetics and toxicology, and activities of current antisense drugs. This volume addresses advances in a broad range of disciplines and is an excellent resource for basic researchers and applied investigators in pharmaceutical laboratories and in such fields as biochemistry and molecular biology.

Author : Patrick Couvreur,Claude Malvy
Publisher : CRC Press
Page : 350 pages
File Size : 47,7 Mb
Release : 2003-09-02
Category : Medical
ISBN : 9780203305669

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Pharmaceutical Aspects of Oligonucleotides by Patrick Couvreur,Claude Malvy Pdf

Oligonucleotides diffuse poorly through biological barriers, including cell membranes. They are also rapidly degraded in vivo by nucleuses. Aiming to improve the administration of compounds, the book studies the development of nucleotide chemistry.

Author : Harri Lönnberg
Publisher : Walter de Gruyter GmbH & Co KG
Page : 474 pages
File Size : 50,8 Mb
Release : 2020-08-10
Category : Science
ISBN : 9783110613988

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Chemistry of Nucleic Acids by Harri Lönnberg Pdf

Life in all its forms is based on nucleic acids which store and transfer genetic information. The book addresses the main aspects of synthesis, hydrolytic stability, solution equilibria of nucleosides and nucleotides as well as base modifications of nucleic acids. The author further describes their structural analogues used as therapeutic drugs, such as antivirals and anticancer agents, and prodrug strategies of nucleotides.

Author : Sudhir Agrawal
Publisher : Humana Press
Page : 377 pages
File Size : 46,9 Mb
Release : 1993-11-30
Category : Science
ISBN : 0896032523

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Protocols for Oligonucleotide Conjugates by Sudhir Agrawal Pdf

You will easily synthesize and analyze oligonucleotide conjugates by following the step-by-step protocols presented in this volume. These techniques are widely used by all molecular biologists and antisense researchers and find special application by pharmacologists working in new drug development and quality assurance assay.

Author : Kathrin Susanne Schmidt
Publisher : Unknown
Page : 157 pages
File Size : 50,8 Mb
Release : 2001
Category : Electronic
ISBN : 3897227029

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Solution- and Solid-phase Synthesis of Monofunctionally Trans-Pt(II) Modified Oligonucleotides and Oligonucleotide Analogues and Their Potential Application in Antigene and Antisense Strategy by Kathrin Susanne Schmidt Pdf

Author : Jens Kurreck
Publisher : Royal Society of Chemistry
Page : 362 pages
File Size : 53,6 Mb
Release : 2008
Category : Medical
ISBN : 9780854041169

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Therapeutic Oligonucleotides by Jens Kurreck Pdf

This book provides a compelling overall update on current status of RNA interference

Author : Lucy Andrews,Trygve O. Tollefsbol
Publisher : Springer Science & Business Media
Page : 197 pages
File Size : 55,9 Mb
Release : 2007-11-29
Category : Medical
ISBN : 9781588296832

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Telomerase Inhibition by Lucy Andrews,Trygve O. Tollefsbol Pdf

This volume presents a compendium of the most recent and advanced methods applied to the rapidly expanding field of telomerase inhibition. The techniques described provide the researcher with a diverse and comprehensive set of tools for the study of telomerase inhibition. The volume is aimed at biochemists, molecular biologists, cancer researchers, and geneticists.

Author : Anant R. Kapdi,Debabrata Maiti,Yogesh S. Sanghvi
Publisher : Elsevier
Page : 358 pages
File Size : 45,6 Mb
Release : 2018-06-04
Category : Science
ISBN : 9780128112939

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Palladium-Catalyzed Modification of Nucleosides, Nucleotides and Oligonucleotides by Anant R. Kapdi,Debabrata Maiti,Yogesh S. Sanghvi Pdf

Palladium-Catalyzed Modification of Nucleosides, Nucleotides and Oligonucleotides describes the procedures and protocols related to the modification of nucleosides, nucleotides and oligonucleotides via Pd-mediated cross-coupling processes. The book highlights the growing area of nucleic acid modification and how Pd-mediated coupling reactions can assist this development. Users will find key synthetic protocols for these reactions in this latest volume in the Latest Trends in Palladium Chemistry series. As most of the research in the field of antiviral agents has centered on the use of modified nucleosides that have exhibited promising activity, this book provides an up-to-date reference for both professionals in industry and other interested parties. Provides synthetic routes for useful nucleoside molecules, information otherwise found only through time-consuming literature searches Covers metal-mediated and metal-catalyzed cross coupling processes of nucleosides and related compounds Includes Suzuki-Miyaura, Stille and Sonogashira reactions, as well as C-H bond functionalization Highlights the growing area of nucleic acid modification and how Pd-mediated coupling reactions can assist