Partitioning In Aqueous Two Phase System

Partitioning In Aqueous Two Phase System Book in PDF, ePub and Kindle version is available to download in english. Read online anytime anywhere directly from your device. Click on the download button below to get a free pdf file of Partitioning In Aqueous Two Phase System book. This book definitely worth reading, it is an incredibly well-written.

Partitioning In Aqueous Two – Phase System

Harry Walter
Partitioning In Aqueous Two Phase System Book PDF

Author : Harry Walter
Publisher : Elsevier
Page : 730 pages
File Size : 42,7 Mb
Release : 2012-12-02
Category : Science
ISBN : 9780323142915

Get Book

Partitioning In Aqueous Two – Phase System by Harry Walter Pdf

Partitioning in Aqueous Two-Phase Systems: Theory, Methods, Uses, and Applications to Biotechnology is a collection of papers that discusses the applications of aqueous two-phase systems to problems of separation and extraction of macromolecules, organelles, and cells. Papers focus on the theoretical basis and the practical details of the procedures used. Some of the papers describe in one or a few steps how two components can be separated by the investigator manipulating their partitions so that one component is in one phase and the other component is in the other phase or at the interface. Investigators can also avail of developed batch extractions for plant organelles, cell membranes, nucleic acids, and proteins. The book cites as an example the partitioning of right-side-out and inside-out vesicles (obtained from fragments of thylakoid membranes) to the top and bottom phases, respectively, of a Dx-PEG system. Other papers describe the use of the countercurrent distribution when single extraction steps are not sufficient to produce a separation in materials that do not differ greatly in their partitioning behavior. The collection can prove valuable for bio-chemists, cellular biologists, micro-biologists, and developmental biologists.

Aqueous Two-Phase Systems

Rajni Hatti-Kaul
Aqueous Two Phase Systems Book PDF

Author : Rajni Hatti-Kaul
Publisher : Springer Science & Business Media
Page : 442 pages
File Size : 42,5 Mb
Release : 2008-02-05
Category : Science
ISBN : 9781592590285

Get Book

Aqueous Two-Phase Systems by Rajni Hatti-Kaul Pdf

A mixture of two polymers, or one polymer and a salt, in an aqueous medium separates into two phases: this phenomenon is useful in biotechn- ogy for product separations. Separation of biological molecules and particles in these aqueous two-phase systems (ATPS) was initiated over 40 years ago by P.-Å. Albertsson, and later proved to be of immense utility in biochemical and cell biological research. A boost in the application of ATPS was seen when problems of separations in biotechnology processes were encountered. Its simplicity, biocompatibility, and amenability to easy scaleup operations make the use of ATPS very attractive for large-scale bioseparations. Despite the advantages ATPS enjoys over other separation techniques, the application of two-phase systems has for a long time been confined to selected labora- ries. Recent years have, however, shown a trend in which increasing numbers of researchers employ two-phase partitioning techniques in both basic and applied research.

Aqueous Two-Phase Partitioning

Boris Y. Zaslavsky
Aqueous Two Phase Partitioning Book PDF

Author : Boris Y. Zaslavsky
Publisher : CRC Press
Page : 720 pages
File Size : 45,6 Mb
Release : 1994-11-15
Category : Science
ISBN : 0824794613

Get Book

Aqueous Two-Phase Partitioning by Boris Y. Zaslavsky Pdf

Covers the fundamental principles of solute partitioning in aqueous two-phase systems, explains their important practical features, and furnishes methods of characterization. The information provided by the partition behaviour of a solute in an aqueous two-phase system is examined.

Aqueous Two-Phase Systems

Harry Walter,Göte Johansson
Aqueous Two Phase Systems Book PDF

Author : Harry Walter,Göte Johansson
Publisher : Elsevier
Page : 768 pages
File Size : 54,8 Mb
Release : 1994-04-18
Category : Nature
ISBN : 0121821293

Get Book

Aqueous Two-Phase Systems by Harry Walter,Göte Johansson Pdf

General methodology and apparatus: phase diagrams, preparation and analysis of two-phase systems, partioning and affinity partitioning of macromolecules: Proteins, nucleic acids, studies on protein interactionsmolecular structure, charge, hydrophobicity, and conformational chan ges, partitioning and affinity partitioning of particulates,organellesseparation and subfractionation, menbrane: separation and subfractionation, membrane domain analysis, aqueous phase separation in biologicalsystems, aqueous two-phase systems in large-scale process biotechnolo gy, proteins; downstream processing, design of proteins for enhanced extraction, other applications of aqueous phases in biotechnology. Enzymology.

Protein Structure Analysis

Roza Maria Kamp,Theodora Choli-Papadopoulou,Brigitte Wittmann-Liebold
Protein Structure Analysis Book PDF

Author : Roza Maria Kamp,Theodora Choli-Papadopoulou,Brigitte Wittmann-Liebold
Publisher : Springer Science & Business Media
Page : 311 pages
File Size : 41,9 Mb
Release : 2012-12-06
Category : Science
ISBN : 9783642592195

Get Book

Protein Structure Analysis by Roza Maria Kamp,Theodora Choli-Papadopoulou,Brigitte Wittmann-Liebold Pdf

"Protein Structure Analysis - Preparation and Characterization" is a compilation of practical approaches to the structural analysis of proteins and peptides. Here, about 20 authors describe and comment on techniques for sensitive protein purification and analysis. These methods are used worldwide in biochemical and biotechnical research currently being carried out in pharmaceu tical and biomedical laboratories or protein sequencing facilities. The chapters have been written by scientists with extensive ex perience in these fields, and the practical parts are well documen ted so that the reader should be able to easily reproduce the described techniques. The methods compiled in this book were demonstrated in student courses and in the EMBO Practical Course on "Microsequence Analysis of Proteins" held in Berlin September 10-15, 1995. The topics also derived from a FEBS Workshop, held in Halkidiki, Thessaloniki, Greece, in April, 1995. Most of the authors participated in these courses as lecturers and tutors and made these courses extremely lively and successful. Since polypeptides greatly vary depending on their specific structure and function, strategies for their structural analysis must for the most part be adapted to each individual protein. Therefore, advantages and limitations of the experimen tal approaches are discussed here critically, so that the reader becomes familiar with problems that might be encountered.

Aqueous Two-Phase Systems

Rajni Hatti-Kaul
Aqueous Two Phase Systems Book PDF

Author : Rajni Hatti-Kaul
Publisher : Unknown
Page : 128 pages
File Size : 53,5 Mb
Release : 2000
Category : Electronic
ISBN : OCLC:748539223

Get Book

Aqueous Two-Phase Systems by Rajni Hatti-Kaul Pdf

Rajni Hatti-Kaul and her expert coauthors combine theory, methodology, and applications in a practical collection of easily reproducible protocols for bioseparations in aqueous two-phase systems (ATPS). The protocols range from established methods to cutting-edge techniques with potential biotechnological applications. Among the methods detailed are those for ATPS preparation and characterization, for partitioning applied to soluble molecules and particulates (including whole cells, membranes, and organelles), and for the isolation and purification of proteins - including a glimpse of large-scale handling of two-phase separations. Practical and informative, with its detailed guidelines allowing researchers to adapt specific systems to their own separation needs, Aqueous Two-Phase Systems: Methods and Protocols demonstrates the scope and utility of two-phase aqueous systems in both basic and applied research.

Intensification of Biobased Processes

Andrzej Gorak,Andrzej Stankiewicz
Intensification of Biobased Processes Book PDF

Author : Andrzej Gorak,Andrzej Stankiewicz
Publisher : Royal Society of Chemistry
Page : 518 pages
File Size : 53,7 Mb
Release : 2018-06-18
Category : Science
ISBN : 9781788010320

Get Book

Intensification of Biobased Processes by Andrzej Gorak,Andrzej Stankiewicz Pdf

In recent years bioprocessing has increased in popularity and importance, however, bioprocessing still poses various important techno-economic and environmental challenges, such as product yields, excessive energy consumption for separations in highly watery systems, batch operation or the downstream processing bottlenecks in the production of biopharmaceutical products. Many of those challenges can be addressed by application of different process intensification technologies discussed in the present book. The first book dedicated entirely to this area, Intensification of Biobased Processesprovides a comprehensive overview of modern process intensification technologies used in bioprocessing. The book focusses on four different categories of biobased products: bio-fuels and platform chemicals; cosmeceuticals; food products; and polymers and advanced materials. It will cover various intensification aspects of the processes concerned, including (bio)reactor intensification; intensification of separation, recovery and formulation operations; and process integration. This is an invaluable source of information for researchers and industrialists working in chemical engineering, biotechnology and process engineering.

Aqueous Two-Phase Systems for Bioprocess Development for the Recovery of Biological Products

Marco Rito-Palomares,Jorge Benavides
Aqueous Two Phase Systems for Bioprocess Development for the Recovery of Biological Products Book PDF

Author : Marco Rito-Palomares,Jorge Benavides
Publisher : Springer
Page : 191 pages
File Size : 47,6 Mb
Release : 2017-07-18
Category : Technology & Engineering
ISBN : 9783319593098

Get Book

Aqueous Two-Phase Systems for Bioprocess Development for the Recovery of Biological Products by Marco Rito-Palomares,Jorge Benavides Pdf

This comprehensive and unique text presents a full overview of downstream processing useful for those new to the concept as well as professionals with experience in the area. The history and theoretical principles of Aqueous Two-Phase Systems (ATPS) are covered in depth. Information on ATPS characterization and application is included, and ATPS equilibria and system parameters that have significant effect on partition behavior are studied. Aqueous Two-Phase Systems for Bioprocess Development for the Recovery of Biological Products addresses specific applications of ATPS for the recovery and partial purification of high molecular weight compounds such as proteins, nucleic acids and polysaccharides, particulate bioproducts such as cells and organelles and low molecular weight compounds. Non-conventional strategies involving ATPS such as affinity systems, continuous liquid-liquid fractionation stages and the recovery from plant extracts are presented. Economic analysis of the application of ATPS in comparison to other fractionation techniques, particularly liquid chromatography, is considered, as are opportunity and current trends in the ATPS research area. Each chapter utilizes the contributors' experimental expertise in traditional and non-conventional ATPS strategies, as well as analysis of areas of opportunity and perspectives on the development and future applications of ATPS in both the lab and larger scale operations. The result is a thorough and singular overview of ATPS which has not been matched by any other text on the market.

Partition of Cell Particles and Macromolecules

Per-Ake Albertsson
Partition of Cell Particles and Macromolecules Book PDF

Author : Per-Ake Albertsson
Publisher : Wiley-Interscience
Page : 346 pages
File Size : 48,7 Mb
Release : 1986-03-28
Category : Science
ISBN : 0471828203

Get Book

Partition of Cell Particles and Macromolecules by Per-Ake Albertsson Pdf

Describes partition techniques for the separation and purification of cells, cell organelles, membrane vesicles, viruses, and biopolymers, such as proteins and nucleic acids. The basic theory of partition is discussed, as are the properties of aqueous, two-phase systems and the general behavior of particles and molecules. Updates include advances and new applications introduced since 1971.

Three Phase Partitioning

Munishwar Nath Gupta,Ipsita Roy
Three Phase Partitioning Book PDF

Author : Munishwar Nath Gupta,Ipsita Roy
Publisher : Elsevier
Page : 346 pages
File Size : 42,8 Mb
Release : 2021-08-11
Category : Science
ISBN : 9780323859035

Get Book

Three Phase Partitioning by Munishwar Nath Gupta,Ipsita Roy Pdf

Three Phase Partitioning: Applications in Separation and Purification of Biological Molecules and Natural Products presents applications in diverse areas of both chemical technology and biotechnology. This book serves as a single resource for learning about both the economical, facile and scalable processes, along with their potential for applications in the separation and purification of materials and compounds across the entire spectra of chemical and biological nature. The book begins by explaining the origins and fundamentals of TPP and continues with chapters on related applications, ranging from the purification of parasite recombinant proteases to oil extraction from oilseeds and oleaginous microbes, and more. Written by researchers who have been pioneers in developing and utilizing three phase partitioning Focuses on applications, with chapters detailing relevance to a wide variety of areas and numerous practical examples Designed to give laboratory workers the information needed to undertake the challenge of designing successful three-phase partitioning protocols

Ionic-Liquid-Based Aqueous Biphasic Systems

Mara G. Freire
Ionic Liquid Based Aqueous Biphasic Systems Book PDF

Author : Mara G. Freire
Publisher : Springer
Page : 315 pages
File Size : 43,9 Mb
Release : 2016-10-05
Category : Technology & Engineering
ISBN : 9783662528754

Get Book

Ionic-Liquid-Based Aqueous Biphasic Systems by Mara G. Freire Pdf

This book offers comprehensive information on the fundamentals and applications of ionic-liquid-based aqueous biphasic systems, which have predominantly (and successfully) been employed as alternative platforms for the extraction, separation and purification of diverse high-value products. The book consists of an initial introduction providing a brief overview, from fundamentals to applications, followed by nine chapters addressing the respective phase diagrams (interpretation and characterization) and remarkable examples of their applications. It also includes two final chapters focusing on recent developments in the search for more environmentally-benign and biocompatible ionic-liquid-based aqueous biphasic systems, and on the progress made to date concerning the recovery, recycling and reuse of the phase-forming components, the goal being the development of cost-effective and sustainable processes. The book offers an interesting and useful guide for a broad readership in the fields of green chemistry, biotechnology, chemical engineering, and biochemistry, among others. Mara G. Freire is a Coordinator Researcher at CICECO - Aveiro Institute of Materials, Chemistry Department, University of Aveiro, Portugal.

Separations Using Aqueous Phase Systems

D. Fisher
Separations Using Aqueous Phase Systems Book PDF

Author : D. Fisher
Publisher : Springer Science & Business Media
Page : 476 pages
File Size : 47,6 Mb
Release : 2012-12-06
Category : Science
ISBN : 9781468456677

Get Book

Separations Using Aqueous Phase Systems by D. Fisher Pdf

The use of aqueous two-phase systems for the partitioning of macromolecules, organelles and cells was originally developed by Per-Ake Albertsson in Sweden in the mid-fifties [1-3]. These systems were initially applied to separations of plant organelles and viruses but their use has now extended into most areas of cell biology and biochemistry [4,5]. Since 1979 biennial International Conferences on Partitioning in Aqueous Two-Phase Systems have been held in Los Angeles (1979), Sheffield (1981), Vancouver (1983) and Lund (1985). The 5th conference was held in Oxford from 23-28 August 1987 and was entitled "Advances in Separations Using Aqueous Phase Systems in Cell Biology and Biotechnology". It is the formal presentations from this meeting which comprise this volume. In contrast to earlier books on phase partitioning [4,5] this volume contains, for the first time, worldwide contributions from over sixty partitioners from a variety of scientific disciplines, thereby providing a detailed overview of the widespread application and potential of bioseparations using phase partitioning. Disciplines include Biophysics, Biochemistry, Cell Biology, Microbiology, Biotechnology and Process Engineering, in both academic and commercial establishments. These biennial conferences allow advances in these diverse partitioning fields to be reviewed and compared; they also provide an opportunity for those considering using phase partitioning to obtain information, advice and contacts. Attendance has grown steadily over the years and 140 scientists came to Oxford. The conference consisted of ten symposia on areas of application of partitioning which have been organised as specific chapters in this volume.

Heme, Chlorophyll, and Bilins

Alison Smith,Michael Witty
Heme Chlorophyll and Bilins Book PDF

Author : Alison Smith,Michael Witty
Publisher : Springer Science & Business Media
Page : 341 pages
File Size : 54,8 Mb
Release : 2002
Category : Science
ISBN : 9781588291110

Get Book

Heme, Chlorophyll, and Bilins by Alison Smith,Michael Witty Pdf

Although researchers can profitably investigate heme, chlorophyll, and related tetrapyrroles in a wide range of academic and medical research programs, the handling and manipulation of these delicate compounds requires considerable skill and cross-boundary knowledge. In Heme, Chlorophyll, and Bilins: Methods and Protocols, an interdisciplinary panel of hands-on investigators overcomes these limitations by describing in detail how to work successfully with chlorophyll, heme, and bilins in biological, medical, chemical, and biochemical research. Each method is presented by a researcher who actually uses it on a daily basis and includes step-by-step instructions and pertinent tricks-of-the-trade that often make the difference between laboratory success and failure. Topics range from methods for the analysis of tetrapyrroles, heme, and hemoproteins, to the biosynthesis and the analysis of chlorophyll and bilins. Timely and highly practical, Heme, Chlorophyll, and Bilins: Methods and Protocols is a gold-standard collection of readily reproducible techniques suitable for a wide range of researchers, whether it be a clinician studying photodynamic therapy, an ecologist studying the chlorophyll composition of leaves in a tropical forest, or a cell biologist investigating the function of specific hemoproteins.

Liquid Biphasic System

Pau Loke Show,Sze Ying Lee,Kit Wayne Chew
Liquid Biphasic System Book PDF

Author : Pau Loke Show,Sze Ying Lee,Kit Wayne Chew
Publisher : Elsevier
Page : 330 pages
File Size : 43,5 Mb
Release : 2020-11-28
Category : Technology & Engineering
ISBN : 9780128223987

Get Book

Liquid Biphasic System by Pau Loke Show,Sze Ying Lee,Kit Wayne Chew Pdf

Downstream bioprocesses have a significant role to play in the creation of a sustainable bio-based economy, enabling the creation of new products and systems from the more sustainable bioprocessing of natural products. Liquid Biphasic System: Fundamentals, Methods, and Applications in Bioseparation Technology explores in detail the fundamental processes and applications of this new separation system, aiding understanding of the basic principles of the technique and offering constructive criticisms on the latest findings. Including coverage of the background, principles, mechanisms, and applications, Liquid Biphasic System addresses how to adapt the technology for the purification of useful compounds with greater cost efficiency and greener processing. It is essential reading for bioprocess engineers, biochemical engineers, biosystem engineers, chemists and microbiologists working in the fields of bioprocessing. Researchers, scientists, and engineers concerned with the selection and evaluation of alternative bioseparation processes will find the book particularly useful. Provides information and examples of advanced separations in a single source Includes detailed descriptions of novel bioseparation systems Covers the latest technologies related to advanced liquid–liquid separation and their applications in various industries

Microcompartmentation and Phase Separation in Cytoplasm

Anonim
Microcompartmentation and Phase Separation in Cytoplasm Book PDF

Author : Anonim
Publisher : Academic Press
Page : 352 pages
File Size : 46,8 Mb
Release : 1999-10-21
Category : Science
ISBN : 0080857310

Get Book

Microcompartmentation and Phase Separation in Cytoplasm by Anonim Pdf

International Review of Cytology presents current advances and comprehensive reviews in cell biology-both plant and animal. Articles address structure and control of gene expression, nucleocytoplasmic interactions, control of cell development and differentiation, and cell transformation and growth. Authored by some of the foremost scientists in the field, each volume provides up-to-date information and directions for future research. This volume provides an overview of major cytoplasmic properties and events which including cytoarchitecture and the physical properties of cytoplasm, molecular compartmentation and gradients, channeling, sorting, and trafficking. It also addresses physicochemical events, both measured and anticipated, which attend solutions under conditions prevailing in cytoplasm: molecular crowding. It summarizes the current state of knowledge in the field and considers questions such as how molecules in cytoplasm interact.