Pcr In Neuroscience

Author : Anonim
Publisher : Elsevier
Page : 455 pages
File Size : 54,6 Mb
Release : 1995-04-13
Category : Science
ISBN : 008053645X

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PCR in Neuroscience by Anonim Pdf

The volumes in this series include contemporary techniques significant to a particular branch of neuroscience. They are an invaluable aid to the student as well as the experienced researcher not only in developing protocols in neuroscience but in disciplines where research is becoming closely related to neuroscience. Each volume of Methods in Neurosciences contains an index, and each chapter includes references. Dr. Conn became Editor-in-Chief of the series beginning with Volume 15, so each subsequent volume could be guest-edited by an expert in that specific field. This further strengthens the depth of coverage in Methods in Neurosciences for students and researchers alike. Direct application of PCR to fresh or frozen clinical specimens (e.g., blood and solid tissue) Complete retrieval of novel expressed genes by PCR without screening a library Quantitation by PCR Mutagenesis by PCR PCR in AIDS research Simple and effective protocols for PCR on archival specimens

Author : James H. Eberwine,Karen L. Valentino,Jack D. Barchas
Publisher : Unknown
Page : 230 pages
File Size : 47,7 Mb
Release : 1994
Category : In situ hybridization
ISBN : 0195075072

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In Situ Hybridization in Neurobiology by James H. Eberwine,Karen L. Valentino,Jack D. Barchas Pdf

In situ hybridization has become an important and widely used research tool for neurobiologists since the authors' first book on the subject, In Situ Hybridization: Applications to Neurobiology (Oxford, 1987). This new volume examines the methodological advances that have been made in in situhybridization techniques and their impact on the study of the nervous system. These include methods for improving sensitivity, ways of examining multiple mRNAs within the same section, ways of examining both proteins and mRNAs within the same section, and methods for using in situ hybridizationtechnology to examine areas of cellular biological significance, such as translational control. The development of alternative labels to radioactivity for the detection of hybridized probes, and the combined use of in situ hybridization and immunocytochemistry is also covered in this book. Itdiscusses the quantification of in situ hybridization signal and presents an overview of information generated by using in situ hybridization in diagnostic medicine. This volume will be essential to researchers in the neurosciences as well as those in other disciplines.

Author : John T. Corthell
Publisher : Academic Press
Page : 124 pages
File Size : 49,8 Mb
Release : 2014-04-16
Category : Science
ISBN : 9780128015278

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Basic Molecular Protocols in Neuroscience: Tips, Tricks, and Pitfalls by John T. Corthell Pdf

Basic Neuroscience Protocols: Tips, Tricks, and Pitfalls contains explanatory sections that describe the techniques and what each technique really tells the researcher on a scientific level. These explanations describe relevant controls, troubleshooting, and reaction components for some of the most widely used neuroscience protocols that remain difficult for many neuroscientists to implement successfully. Having this additional information will help researchers ensure that their experiments work the first time, and will also minimize the time spent working on a technique only to discover that the problem was them, and not their materials. Describes techniques in very specific detail with step-by-step instructions, giving researchers in-depth understanding Offers many details not present in other protocol books Describes relevant controls for each technique and what those controls mean Chapters include references (key articles, books, protocols) for additional study Describes both the techniques and the habits necessary to get quality results, such as aseptic technique, aliquoting, and general laboratory rules

Author : Eva Harris
Publisher : Oxford University Press
Page : 327 pages
File Size : 47,6 Mb
Release : 1998-12-31
Category : Science
ISBN : 9780195119268

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A Low-Cost Approach to PCR by Eva Harris Pdf

The polymerase chain reaction (PCR) is a technique used to replicate specific pieces of DNA millions of times, which permits the detection and analysis of minute amounts of nucleic acids. Since its introduction in the late 1980s, this technique has been applied not only in molecular biology research but also in fields as diverse as anthropology, phylogeny, and forensics. However, despite the large impact of PCR, many of its applications remain within the confines of research and the academic environment. Now, in A Low-Cost Approach to PCR: Appropriate Transfer of Biomolecular Techniques, Dr. Eva Harris makes this elegantly simple technique more accessible to researchers, physicians, and laboratory workers throughout the world. She provides a description of the theoretical basis of the technique, the practical details of the method, and the philosophy behind the technology transfer program that she developed over the last ten years. The book serves as a guide for potential users in developing countries and for scientists in developed countries who may wish to work abroad. In addition, the low-cost approach outlined in this book can be useful for high school, undergraduate, or continuing education programs in the United States. While the specific applications of PCR outlined in the book are immediately useful to the study of infectious diseases, the approach presented can be generalized to a number of other technologies and situations. The book will help laboratories in many areas of the world generate information on site for use by physicians, epidemiologists, public health workers, and health policy professionals to develop new strategies for disease control.

Author : Rosemary Martin
Publisher : CRC Press
Page : 280 pages
File Size : 48,6 Mb
Release : 2019-08-16
Category : Science
ISBN : 9781000725353

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Neuroscience Methods by Rosemary Martin Pdf

Unique in its coverage of such an extensive range of methods, Neuroscience Methods: A Guide for Advanced Students provides easy-to-understand descriptions of the many different techniques that are currently being used to study the brain at the molecular and cellular levels. This valuable reference text will help rescue undergraduate and postgraduate students from continuing bewilderment at the methods sections of current neuroscience publications. Topics covered include in vivo and in vitro preparations, electrophysiological, histochemical, hybridization and genetic techniques, measurement of cellular ion concentrations, methods of drug application, production of antibodies, expression systems, and neural grafting.

Author : Jerry J. Buccafusco
Publisher : CRC Press
Page : 341 pages
File Size : 46,8 Mb
Release : 2000-08-29
Category : Medical
ISBN : 9781420041811

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Methods of Behavior Analysis in Neuroscience by Jerry J. Buccafusco Pdf

Using the most well-studied behavioral analyses of animal subjects to promote a better understanding of the effects of disease and the effects of new therapeutic treatments on human cognition, Methods of Behavior Analysis in Neuroscience provides a reference manual for molecular and cellular research scientists in both academia and the pharmaceutic

Author : Huangui Xiong,Howard E. Gendelman
Publisher : Springer
Page : 0 pages
File Size : 44,6 Mb
Release : 2013-11-05
Category : Medical
ISBN : 1461487935

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Current Laboratory Methods in Neuroscience Research by Huangui Xiong,Howard E. Gendelman Pdf

Current Laboratory Methods in Neuroscience Research is a research manual for both students and seasoned researchers. It focuses on commonly-used techniques employed in neuroscience research, presented in a simple, step-by-step manner for laboratory use. The manual also offers a “blueprint” for bench-to-bedside research designed to facilitate multidisciplinary neuroscience pursuits. Sections include coverage of neurohistological techniques, in vitro preparations, leukocyte isolation and application in neuroscience, standard laboratory nucleic acid and protein detections, nanomedicine, bioimaging, neuroelectrophysiology, immunohistochemistry and autoradiography, analysis of gene expression, and animal models.

Author : Patrick F. Chinnery
Publisher : Imperial College Press
Page : 468 pages
File Size : 52,5 Mb
Release : 2006
Category : Science
ISBN : 9781860948961

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Neuroscience for Neurologists by Patrick F. Chinnery Pdf

The last decade has seen major advances in our understanding of the basic scientific principles that underpin clinical neurology. Many of these advances have already had a major impact on routine clinical practice, and this is likely to continue in the future. Although this makes it an exciting time to practice neurology, it also presents new challenges. How can established general neurologists keep up-to-date with clinically relevant scientific advances, and how can the specialist remain competent outside his own field? What should trainee neurologists learn to prepare themselves for the future career?. Written by experts in the field, this timely book presents the scientific principles behind neurology. It aims to take the reader from the human genome, through gene expression, to molecular and cellular pathology, and subsequently to contemporary clinical investigations and clinical trials. Each contribution includes a discussion about the future of the field and highlights areas of potential growth and their relevance to routine clinical practice over the next decade. The succinctly written chapters will be easily accessible to both neurologists and trainees who have no expertise in the area. Many of the chapters are co-written by a clinician and a basic scientist, and each has been subject to peer review OCo both by experts in the field and a OC jobbingOCO general neurologist. This has ensured that the contributions reflect contemporary scientific thinking which is presented in a way that is easy to digest. The book includes generous use of diagrams to help explain difficult concepts, a glossary explaining the terminology, and provides information on links to web-based resources for further information. Sample Chapter(s). Chapter 1: Neurogenetics -- Single Gene Disorders (761 KB). Contents: Neurogenetics OCo Single Gene Disorders; Neurogenetics II: Complex Disorders; Functional Genomics and Proteomics: Application in Neurosciences; Mitochondria; Nanotechnology for Neuronal Ion Channels; Molecular and Cellular Pathways of Neurodegeneration in Motor Neurone Disease; Neurodegenerative Disorders: Parkinson''s Disease and Huntington''s Disease; Molecular Pathogenesis of Neuroinflammation; Neurovirology; Molecular Neurology of Prion Disease; Monitoring and Interpretation of Intracranial Pressure; Cerebral Perfusion and Stroke; A Review of Structural Magnetic Resonance Neuroimaging; Applications of Positron Emission Tomography (PET) in Neurology; Functional Magnetic Resonance Imaging; How to Spot Bias and Other Potential Problems in Randomised Controlled Trials. Readership: Neurologists, trainees/interns in neurology and related disciplines, neurobiologists."

Author : Michael A. Innis,David H. Gelfand,John J. Sninsky
Publisher : Academic Press
Page : 566 pages
File Size : 41,5 Mb
Release : 1999-05-11
Category : Science
ISBN : 9780080919638

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PCR Applications by Michael A. Innis,David H. Gelfand,John J. Sninsky Pdf

PCR is the most powerful technique currently used in molecular biology. It enables the scientist to quickly replicate DNA and RNA on the benchtop. From its discovery in the early 80's, PCR has blossomed into a method that enables everything from ready mutation of DNA/RNA to speedy analysis of tens of thousands of nucleotide sequences daily. PCR Applications examines the latest developments in this field. It is the third book in the series, building on the previous publications PCR Protocols and PCR Strategies. The manual discusses techniques that focus on gene discovery, genomics, and DNA array technology, which are contributing factors to the now-occurring bioinformatics boom. Key Features * Focuses on gene discovery, genomics, and DNA array technology * Covers quantitative PCR techniques, including the use of standards and kinetic analysis includes statistical refinement of primer design parameters * Ilustrates techniques used in microscopic tissue samples, such as single cell PCR, whole cell PCR, laser capture microdissection, and in situ PCR Entries provide information on: * Nomenclature * Expression * Sequence analysis * Structure and function * Electrophysiology * Parmacology * Information retrieval

Author : Sarah Maddocks,Rowena Jenkins
Publisher : Academic Press
Page : 98 pages
File Size : 45,5 Mb
Release : 2016-10-27
Category : Science
ISBN : 9780128026977

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Understanding PCR by Sarah Maddocks,Rowena Jenkins Pdf

Understanding PCR: A Practical Bench-Top Guide gives you all of the information you need to plan your first PCR, from reagents to conditions to analysis and beyond. It is a user friendly book that has step-by-step basic protocols, which can be adapted to your needs. Includes helpful information such as where to order your reagents and basic troubleshooting hints and tips. Includes resources for reagents Explains basic laboratory preparation Provides straightforward experimental protocols Incorporates fundamental analytical techniques Contains a troubleshooting guide

Author : Larry Ernest Davis,Peter G. E. Kennedy
Publisher : Butterworth-Heinemann
Page : 572 pages
File Size : 54,7 Mb
Release : 2000
Category : Central nervous system
ISBN : UOM:39015042574577

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Infectious Diseases of the Nervous System by Larry Ernest Davis,Peter G. E. Kennedy Pdf

This is a current review of neurological infections and management options.

Author : M Dorak
Publisher : Garland Science
Page : 484 pages
File Size : 52,8 Mb
Release : 2007-01-24
Category : Medical
ISBN : 9781134183999

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Real-time PCR by M Dorak Pdf

With a variety of detection chemistries, an increasing number of platforms, multiple choices for analytical methods and the jargon emerging along with these developments, real-time PCR is facing the risk of becoming an intimidating method, especially for beginners. Real-time PCR provides the basics, explains how they are exploited to run a real-time PCR assay, how the assays are run and where these assays are informative in real life. It addresses the most practical aspects of the techniques with the emphasis on 'how to do it in the laboratory'. Keeping with the spirit of the Advanced Methods Series, most chapters provide an experimental protocol as an example of a specific assay.

Author : Jacqueline N. Crawley,Charles Gerfen,Ron McKay,Michael Rogawski,David R. Sibley
Publisher : Current Protocols
Page : 128 pages
File Size : 55,8 Mb
Release : 1997-06-01
Category : Medical
ISBN : 0471163619

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Current Protocols in Neuroscience by Jacqueline N. Crawley,Charles Gerfen,Ron McKay,Michael Rogawski,David R. Sibley Pdf

Current Protocols in Neuroscience (CPN) draws from techniques in molecular neurobiology, neurophysiology, neuroanatomy, neuropharmacology, and behavioral neuroscience to meet the specific needs of researchers in the full range of disciplines that is involved in studying the brain, nervous system, and corresponding behaviors. The editorial board of CPN have assembled an outstanding range of methods to enable users to explore their fields in greater depth and branch into related areas. The one-volume, looseleaf manual features carefully edited techniques with authors' troubleshooting tips and helpful comments that come from extensive experience in using these procedures. Quarterly updates, filed into the looseleaf, keep you and your laboratory current with the latest developments in this rapidly changing field. The initial purchase includes one year of updates and then subscribers may renew their annual subscriptions. Current Protocols publishes a family of laboratory manuals for bioscientists, including Molecular Biology, Immunology, Human Genetics, Protein Science, Cytometry, Cell Biology, Pharmacology, and Toxicology.

Author : Michael A. Innis,David H. Gelfand,John J. Sninsky
Publisher : Elsevier
Page : 373 pages
File Size : 46,7 Mb
Release : 1995-07-06
Category : Science
ISBN : 9780080538549

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PCR Strategies by Michael A. Innis,David H. Gelfand,John J. Sninsky Pdf

PCR Strategies expands and updates the landmark volume PCR Protocols. It is a companion laboratory manual that provides a completely new set of up-to-date strategies and protocols for getting the most from PCR. The editors have organized the book into four sections, focusing on principles, analyses, research applications, and alternative strategies for a wide variety of basic and clinical needs. If you own PCR Protocols, you will want PCR Strategies. If you don't own PCR Protocols, you will want to buy both! Concepts explained Methods detailed Trouble-shooting emphasized Novel applications highlighted Key concepts for PCR Analysis of PCR products Research applications Alternative amplification strategies

Author : Kirstin J. Edwards,Julie M. J. Logan,Nick A. Saunders
Publisher : Taylor & Francis
Page : 362 pages
File Size : 48,5 Mb
Release : 2004
Category : Polymerase chain reaction
ISBN : 9781134184002

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Real-Time PCR by Kirstin J. Edwards,Julie M. J. Logan,Nick A. Saunders Pdf