Pcr Applications

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PCR Applications

Michael A. Innis,David H. Gelfand,John J. Sninsky
PCR Applications Book PDF

Author : Michael A. Innis,David H. Gelfand,John J. Sninsky
Publisher : Academic Press
Page : 566 pages
File Size : 44,6 Mb
Release : 1999-05-11
Category : Science
ISBN : 9780080919638

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PCR Applications by Michael A. Innis,David H. Gelfand,John J. Sninsky Pdf

PCR is the most powerful technique currently used in molecular biology. It enables the scientist to quickly replicate DNA and RNA on the benchtop. From its discovery in the early 80's, PCR has blossomed into a method that enables everything from ready mutation of DNA/RNA to speedy analysis of tens of thousands of nucleotide sequences daily. PCR Applications examines the latest developments in this field. It is the third book in the series, building on the previous publications PCR Protocols and PCR Strategies. The manual discusses techniques that focus on gene discovery, genomics, and DNA array technology, which are contributing factors to the now-occurring bioinformatics boom. Key Features * Focuses on gene discovery, genomics, and DNA array technology * Covers quantitative PCR techniques, including the use of standards and kinetic analysis includes statistical refinement of primer design parameters * Ilustrates techniques used in microscopic tissue samples, such as single cell PCR, whole cell PCR, laser capture microdissection, and in situ PCR Entries provide information on: * Nomenclature * Expression * Sequence analysis * Structure and function * Electrophysiology * Parmacology * Information retrieval

PCR Technology

Henry Erlich
PCR Technology Book PDF

Author : Henry Erlich
Publisher : Springer
Page : 246 pages
File Size : 41,7 Mb
Release : 2015-12-31
Category : Science
ISBN : 9781349202355

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PCR Technology by Henry Erlich Pdf

This is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years.

Clinical Applications of PCR

Y. M. Dennis Lo
Clinical Applications of PCR Book PDF

Author : Y. M. Dennis Lo
Publisher : Humana Press
Page : 0 pages
File Size : 45,7 Mb
Release : 1998-03-02
Category : Science
ISBN : 0896034992

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Clinical Applications of PCR by Y. M. Dennis Lo Pdf

The polymerase chain reaction (PCR) is one of the most important molecular biological methods ever devised, with numerous applications to cli- cal molecular medicine. Since its description in 1985, PCR has undergone tremendous improvements, and many variations on the basic PCR theme have been published. With such a large volume of PCR-related literature, a clinical scientist wishing to use the technique will have a difficult task loc- ing the relevant information to implement it effectively. There is thus clearly a need for an up-to-date volume with detailed protocols to facilitate the setting up of those techniques most relevant to clinical applications. Unlike some other books on this topic, Clinical Applications of PCR includes only methods that are of direct relevance in clinical settings. The book is organized in three parts: an introductory section, a section on general methodology, and a final section with specific clinical applications. The first section covers the basic principles of PCR and is most useful to those new to molecular diagnosis. The next chapter includes useful tips for setting up a PCR laboratory. Section 2 then outlines some of the most commonly used PCR-based techniques in molecular diagnosis. Section 3 includes carefully chosen examples that represent typical applications of PCR in diverse clinical fields, encompassing hematology, oncology, genetics, and microbiology.

PCR Protocols

Bruce A. White
PCR Protocols Book PDF

Author : Bruce A. White
Publisher : Springer Science & Business Media
Page : 397 pages
File Size : 55,9 Mb
Release : 2008-02-02
Category : Science
ISBN : 9781592595020

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PCR Protocols by Bruce A. White Pdf

PCR has been successfully utilized in every facet of basic, cli- cal, and applied studies of the life sciences, and the impact that PCR has had on life science research is already staggering. C- comitant with the essentially universal use of PCR has been the creative and explosive development of a wide range of PCR-based techniques and applications. These increasingly numerous pro- cols have each had the general effect of facilitating and acceler- ing research. Because PCR technology is relatively easy and inexpensive, PCR applications are well within the reach of every research lab. In this sense, PCR has become the "equalizer" between "small" and "big" labs, since its use makes certain projects, especially those related to molecular cloning, now far more feasible for the small lab with a modest budget. This new volume on PCR Protocols does not attempt the impossible task of representing all PCR-based protocols. Rather, it presents a range of protocols, both analytical and preparative, that provide a solid base of knowledge on the use of PCR in many c- mon research problems. The first six chapters provide some basic information on how to get started. Chapters 7-19 represent primarily analytical uses of PCR, both for simple DNA and RNA detection, as well as for more complex analyses of nucleic acid (e. g. , DNA footprin ting, RNA splice site localization). The remaining chapters represent "synthetic," or preparative, uses of PCR.

Clinical Applications of PCR

Rajyalakshmi Luthra,Rajesh Singh,Keyur Pravinchandra Patel
Clinical Applications of PCR Book PDF

Author : Rajyalakshmi Luthra,Rajesh Singh,Keyur Pravinchandra Patel
Publisher : Humana
Page : 0 pages
File Size : 47,5 Mb
Release : 2016-02-03
Category : Science
ISBN : 1493933582

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Clinical Applications of PCR by Rajyalakshmi Luthra,Rajesh Singh,Keyur Pravinchandra Patel Pdf

This updated volume explores a wide variety of clinical applications of PCR such as detecting DNA methylation, detection of viruses and protozoa in infectious diseases, estimation of gene copy number aberrations, primer extension coupled with mass spectroscopy, and high throughput NGS techniques. The application of PCR has shown incredible value in the study of genomics and transcriptomics, not only for discovery but also for routine clinical applications, and it forms the cornerstone of personalized medicine. Written for the highly successful Methods in Molecular Biology series, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and authoritative, Clinical Applications of PCR, Third Edition serves as an ideal guide for researchers aiming to understand the principles behind each application and for their implementation in the laboratory.

PCR Protocols

Michael A. Innis,David H. Gelfand,John J. Sninsky,Thomas J. White
PCR Protocols Book PDF

Author : Michael A. Innis,David H. Gelfand,John J. Sninsky,Thomas J. White
Publisher : Academic Press
Page : 482 pages
File Size : 47,8 Mb
Release : 2012-12-02
Category : Science
ISBN : 9780080886718

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PCR Protocols by Michael A. Innis,David H. Gelfand,John J. Sninsky,Thomas J. White Pdf

The correct procedures you need for frustration-free PCR methods and applications are contained in this complete, step-by-step, clearly written, inexpensive manual. Avoid contamination--with specific instructions on setting up your lab Avoid cumbersome molecular biological techniques Discover new applications

Clinical Applications of PCR

Y. M. Dennis Lo
Clinical Applications of PCR Book PDF

Author : Y. M. Dennis Lo
Publisher : Springer Science & Business Media
Page : 204 pages
File Size : 42,5 Mb
Release : 2008-02-04
Category : Science
ISBN : 9781597450744

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Clinical Applications of PCR by Y. M. Dennis Lo Pdf

In this updated second edition, leading researchers apply molecular diagnostics to the many recent advances that have occurred in polymerase chain reaction( PCR)-based technologies. Highlights include real-time PCR, which allows the technique to be performed in a quantitative manner with improved sensitivity, robustness, and resilience to carryover contamination, mass spectrometric analysis of nucleic acids, and circulating cell-free nucleic acids in plasma. The authors apply these innovations to a broad spectrum of applications, including gene expression, methylation, trace molecule, gene dosage, and single cell analysis.

Rapid Cycle Real-Time PCR — Methods and Applications

W. Dietmaier,C. Wittwer,N. Sivasubramanian
Rapid Cycle Real Time PCR Methods and Applications Book PDF

Author : W. Dietmaier,C. Wittwer,N. Sivasubramanian
Publisher : Springer Science & Business Media
Page : 200 pages
File Size : 51,8 Mb
Release : 2013-06-29
Category : Medical
ISBN : 9783642593970

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Rapid Cycle Real-Time PCR — Methods and Applications by W. Dietmaier,C. Wittwer,N. Sivasubramanian Pdf

Rapid-Cycle Real-Time PCR is a powerful technique for nucleic acid amplification and analysis that often requires less than half an hour to perform. Samples are amplified by rapid-cycle PCR followed by immediate melting curve analysis in the same instrument. Melting curve analysis of PCR products with SYBR Green I often allows product identification without gel electrophoresis. Furthermore, in the presence of fluorescent hybridization probes, melting curves provide "dynamic dot blots" for fine sequence analysis, including single nucleotide polymorphisms (SNPs). The method is often cited as the most versatile, efficient method for nucleic acid analysis in research and diagnostics in the fields of genetics and oncology. Molecular diagnostics has never been easier!

Rapid Cycle Real-Time PCR — Methods and Applications

Carl Wittwer,Meinhard Hahn,Karen Kaul
Rapid Cycle Real Time PCR Methods and Applications Book PDF

Author : Carl Wittwer,Meinhard Hahn,Karen Kaul
Publisher : Springer
Page : 223 pages
File Size : 42,9 Mb
Release : 2012-12-06
Category : Science
ISBN : 9783642188404

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Rapid Cycle Real-Time PCR — Methods and Applications by Carl Wittwer,Meinhard Hahn,Karen Kaul Pdf

Rapid Cycle Real-Time PCR is a powerful technique for nucleic acid quantification and analysis that takes less than 30 minutes to complete. Fluorescence is automatically monitored each cycle and the amount of template quantified by advanced analytical methods, such as the second derivative maximum method. Immediately following rapid cycle PCR, melting curve analysis is performed to verify product purity with SYBR Green I and/or genotype with fluorescently-labeled hybridization probes(HybProbes or SimpleProbes). Rapid cycle real-time PCR is often cited as the most versatile, efficient method for nucleic acid quantification in research and climical studies. Molecular analysis has never been easier!

Polymerase Chain Reaction for Biomedical Applications

Ali Samadikuchaksaraei
Polymerase Chain Reaction for Biomedical Applications Book PDF

Author : Ali Samadikuchaksaraei
Publisher : BoD – Books on Demand
Page : 186 pages
File Size : 49,6 Mb
Release : 2016-12-14
Category : Science
ISBN : 9789535127956

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Polymerase Chain Reaction for Biomedical Applications by Ali Samadikuchaksaraei Pdf

Do you want to know the details that should be taken into consideration in order to have accurate conventional and real-time PCR results? If so, this book is for you. Polymerase Chain Reaction for Biomedical Applications is a collection of chapters for both novice and experienced scientists and technologists aiming to address obtaining an optimized real-time PCR result, simultaneous processing of a large number of samples and assays, performing PCR and RT-PCR on cell lysate without extraction of DNA or RNA, detecting false-positive PCR results, detecting organisms in viral and microbial diseases and hospital environment, following safety assessments of food products, and using PCR for introduction of mutations. This is a must-have book for any PCR laboratory.

The PCR Revolution

Stephen A. Bustin
The PCR Revolution Book PDF

Author : Stephen A. Bustin
Publisher : Cambridge University Press
Page : 327 pages
File Size : 51,8 Mb
Release : 2010
Category : Science
ISBN : 9780521882316

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The PCR Revolution by Stephen A. Bustin Pdf

Examines the latest innovations and the overall impact of PCR on areas of molecular research.

PCR Strategies

Michael A. Innis,David H. Gelfand,John J. Sninsky
PCR Strategies Book PDF

Author : Michael A. Innis,David H. Gelfand,John J. Sninsky
Publisher : Elsevier
Page : 373 pages
File Size : 52,7 Mb
Release : 1995-07-06
Category : Science
ISBN : 9780080538549

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PCR Strategies by Michael A. Innis,David H. Gelfand,John J. Sninsky Pdf

PCR Strategies expands and updates the landmark volume PCR Protocols. It is a companion laboratory manual that provides a completely new set of up-to-date strategies and protocols for getting the most from PCR. The editors have organized the book into four sections, focusing on principles, analyses, research applications, and alternative strategies for a wide variety of basic and clinical needs. If you own PCR Protocols, you will want PCR Strategies. If you don't own PCR Protocols, you will want to buy both! Concepts explained Methods detailed Trouble-shooting emphasized Novel applications highlighted Key concepts for PCR Analysis of PCR products Research applications Alternative amplification strategies

PCR

Mike McPherson,Simon Møller
PCR Book PDF

Author : Mike McPherson,Simon Møller
Publisher : Garland Science
Page : 292 pages
File Size : 53,9 Mb
Release : 2007-01-25
Category : Science
ISBN : 9780203002674

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PCR by Mike McPherson,Simon Møller Pdf

A thoroughly updated version of the successful first edition with a new chapter on Real-Time PCR, more prokaryotic applications, and more detail in the complex mutagenesis sections. Information on PCR applications in genomics and proteomics have been expanded and integrated throughout the text. There is also advice on available products and specific pointers to the most appropriate methods. As with the first edition, this will be an ideal practical introduction and invaluable guide to PCR and its applications.

Real-Time PCR

M Tevfik Dorak
Real Time PCR Book PDF

Author : M Tevfik Dorak
Publisher : Taylor & Francis
Page : 362 pages
File Size : 55,9 Mb
Release : 2007-01-24
Category : Medical
ISBN : 9781134184002

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Real-Time PCR by M Tevfik Dorak Pdf

With a variety of detection chemistries, an increasing number of platforms, multiple choices for analytical methods and the jargon emerging along with these developments, real-time PCR is facing the risk of becoming an intimidating method, especially for beginners. Real-time PCR provides the basics, explains how they are exploited to run a real-time PCR assay, how the assays are run and where these assays are informative in real life. It addresses the most practical aspects of the techniques with the emphasis on 'how to do it in the laboratory'. Keeping with the spirit of the Advanced Methods Series, most chapters provide an experimental protocol as an example of a specific assay.

DNA Amplification

Vadim V. Demidov,Natalia E. Broude
DNA Amplification Book PDF

Author : Vadim V. Demidov,Natalia E. Broude
Publisher : Taylor & Francis
Page : 342 pages
File Size : 44,7 Mb
Release : 2004
Category : Science
ISBN : 0954523296

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DNA Amplification by Vadim V. Demidov,Natalia E. Broude Pdf

Whereas most books on DNA amplification focus on PCR-based technologies, this volume presents a wider range of methods to amplify DNA with an emphasis on their diverse applications. The book covers both well-established and newly-developed protocols including ligation-based thermocycling approaches, real-time PCR and other new PCR developments, plus several powerful non-PCR isothermal DNA amplification techniques, for example: real-time strand displacement amplification (SDA), rolling-circle amplification (RCA) and multiple-displacement amplification (MDA). An entire section is devoted to a group of enzymes, both natural and engineered, which are employed for DNA amplification and related purposes. In addition, the use of DNA amplification in the detection of non-DNA analytes is presented.