Peptide Analysis Protocols

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HPLC of Peptides and Proteins

Marie-Isabel Aguilar
HPLC of Peptides and Proteins Book PDF

Author : Marie-Isabel Aguilar
Publisher : Springer Science & Business Media
Page : 411 pages
File Size : 55,7 Mb
Release : 2008-02-03
Category : Science
ISBN : 9781592597420

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HPLC of Peptides and Proteins by Marie-Isabel Aguilar Pdf

The introduction of high-performance liquid chromatography (HPLC) to the analysis of peptides and proteins some 25 years ago revolutionized the biological sciences by enabling the rapid and sensitive analysis of peptide and protein structure through the exquisite speed, sensitivity, and resolution that can be easily obtained. Today, HPLC in its various modes has become the pivotal technique in the characterization of peptides and proteins and currently plays a critical role in both our understanding of biological processes and in the development of peptide- and protein-based pharmaceuticals. The number of applications of HPLC in peptide and protein purification continues to expand at an extremely rapid rate. Solid-phase peptide synthesis and recombinant DNA techniques have allowed the production of large quantities of peptides and proteins that need to be highly purified. HPLC techniques are also used extensively in the isolation and characterization of novel proteins that will become increasingly important in the postgenomic age. The design of multidimensional purification schemes to achieve high levels of product purity further demonstrates the power of HPLC techniques not only in the characterization of cellular events, but also in the production of pepti- and protein-based therapeutics. HPLC continues to be at the heart of the analytical techniques with which scientists in both academia and in industry must arm themselves to be able to fully characterize the identity, purity, and potency of peptides and proteins.

Peptide Analysis Protocols

Ben M. Dunn,Michael W. Pennington
Peptide Analysis Protocols Book PDF

Author : Ben M. Dunn,Michael W. Pennington
Publisher : Unknown
Page : 335 pages
File Size : 51,9 Mb
Release : 1994
Category : Biochemistry
ISBN : 1592595235

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Peptide Analysis Protocols by Ben M. Dunn,Michael W. Pennington Pdf

In this second volume of a two-volume set, state-of-the-art methods are given for the analysis of peptides. Leading authorities provide reproducible protocols for peptide analysis using HPLC, capillary zone electrophoresis, mass spectrometry, UV and fluorescence spectroscopy, to name a few. The authors describe the procedures in a practical, step-by-step fashion suitable for both experts or novices. The chapters also include a helpful notes section offering troubleshooting tips, alternative procedures, details on substituting unavailable materials, and informative explanations about why certain steps are necessary. These extra measures help to guarantee the successful outcome of your experiments.; Dunn and Pennington's Peptide Analysis Protocols offers a comprehensive collection of standard, readily reproducible methods for many procedures that will enable scientists in every area of biochemical and biological research to analyze those peptides that have been synthesized in the laboratory.

Peptide Analysis Protocols

Ben M. Dunn,Michael W. Pennington
Peptide Analysis Protocols Book PDF

Author : Ben M. Dunn,Michael W. Pennington
Publisher : Humana
Page : 335 pages
File Size : 42,5 Mb
Release : 2013-12-06
Category : Science
ISBN : 1489940049

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Peptide Analysis Protocols by Ben M. Dunn,Michael W. Pennington Pdf

As the technology base for the preparation of increasingly c- plex peptides has improved, the methods for their purification and ana- sis have also been improved and supplemented. Peptide science routinely utilizes tools and techniques that are common to organic chemistry, p- tein chemistry, biophysical chemistry, enzymology, pharmacology, and molecular biology. A fundamental understanding of each of these areas is essential for interpreting all of the data that a peptide scientist may see. The purpose of Peptide Analysis Protocols is to provide the novice with sufficient practical information necessary to begin developing useful analysis and separation skills. Understanding and developing these skills will ultimately yield a scientist with broadened knowledge and good problem-solving abilities. Although numerous books that address d- ferent specialties, such as HPLC, FAB-MS, CE, and NMR, have been written, until now no single volume has reviewed all of these techniques with a focus on "getting started" in separation and analysis of peptides. This volume will also provide those who already possess practical knowledge of the more advanced aspects of peptide science with detailed applications for each of these protocols. Because the chapters have been written by researchers active in each of the fields that they discuss, a great deal of information on and insight into solution of real problems that they have encountered is presented. Examplary results are clearly demonstrated and discussed. For more advanced investi- tions, supplementary experiments are often suggested.

Peptide Characterization and Application Protocols

Gregg B. Fields
Peptide Characterization and Application Protocols Book PDF

Author : Gregg B. Fields
Publisher : Humana Press
Page : 342 pages
File Size : 43,8 Mb
Release : 2010-11-19
Category : Science
ISBN : 161737637X

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Peptide Characterization and Application Protocols by Gregg B. Fields Pdf

This book is dedicated to the characterization of peptides and their applications for the study of biochemical systems. The contributing authors are all leaders in the field of peptide research. Part I, Characterization, presents the most recent advances in select analytical techniques. Part II, Application, presents a variety of specific applications for synthetic peptides. This book is an indispensable aid in the pursuit of new directions in peptide research.

Basic Protein and Peptide Protocols

John M. Walker
Basic Protein and Peptide Protocols Book PDF

Author : John M. Walker
Publisher : Humana
Page : 512 pages
File Size : 53,8 Mb
Release : 1994-04-26
Category : Medical
ISBN : UOM:39015026916380

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Basic Protein and Peptide Protocols by John M. Walker Pdf

Basic Protein and Peptide Protocols offers an excellent collection of reproducible, step-by-step laboratory methods covering three major areas: (1) the quantitation and characterization of proteins, (2) the electrophoretic and blotting procedures used in protein isolation and characterization, and (3) the analysis of protein and peptide structure. THOUSANDS of labs are already using Basic Protein and Peptide Protocols-you should be too!

A Practical Guide to Protein and Peptide Purification for Microsequencing

Paul T. Matsudaira
A Practical Guide to Protein and Peptide Purification for Microsequencing Book PDF

Author : Paul T. Matsudaira
Publisher : Elsevier
Page : 205 pages
File Size : 47,9 Mb
Release : 2012-12-02
Category : Science
ISBN : 9780080924618

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A Practical Guide to Protein and Peptide Purification for Microsequencing by Paul T. Matsudaira Pdf

Why a Second Edition? The Second Edition provides practical answers to the general question, "How can I obtain useful sequence information from my protein or peptide?" rather than the more specific question asked in the first edition, "How can I obtain the N-terminal sequence?" Important new methods include ways of dealing with blocked N termini, computer analysis of protein sequences, and the recent revolution in mass spectrometry. Mass spectrophotometric characterization of proteins and peptides N-terminal sequencing of proteins with blocked N termini Internal amino acid sequence analysis after protease digestion in-gel and on-blot Improved microscale peptide purification methods Computer analysis of protein sequences New protocols tested and refined through everyday use in authors' laboratories Updated reference chapter covering all aspects of protein microsequencing

Capillary Electrophoresis of Proteins and Peptides

Nguyet Thuy Tran,Myriam Taverna
Capillary Electrophoresis of Proteins and Peptides Book PDF

Author : Nguyet Thuy Tran,Myriam Taverna
Publisher : Humana
Page : 0 pages
File Size : 49,5 Mb
Release : 2016-07-30
Category : Science
ISBN : 1493940120

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Capillary Electrophoresis of Proteins and Peptides by Nguyet Thuy Tran,Myriam Taverna Pdf

This book provides a comprehensive survey of recent developments and applications of high performance capillary electrophoresis in the field of protein and peptide analysis with a distinct focus on the analysis of intact proteins. With practical detail, the contents cover different modes of capillary electrophoresis (CE) useful for protein and peptide analysis, CZE, CIEF, ACE, CGE, and different types of application such as the quality control of therapeutic proteins and monoclonal antibodies, clinical analyses of chemokines in tissues, qualitative and quantitative analysis of vaccine proteins, and determination of binding constants in complexes involving peptides or proteins. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and exhaustive, Capillary Electrophoresis of Proteins and Peptides: Methods and Protocols serves both beginners and experts with a collection of the current and most active topics in this vital field of study.

The Protein Protocols Handbook

John M. Walker
The Protein Protocols Handbook Book PDF

Author : John M. Walker
Publisher : Springer Science & Business Media
Page : 759 pages
File Size : 46,9 Mb
Release : 1996
Category : Electronic books
ISBN : 9780896033382

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The Protein Protocols Handbook by John M. Walker Pdf

-- 145 concise, step-by-step protocols for the analysis of proteins and peptides -- Provides more than three times as many protocols than the competitors' manuals -- in the incomparable Methods in Molecular Biology "TM" style -- at an affordable price -- Combound in a large 7 x l0 inch format for easy benchtop use -- An indispensable reference for industry-based researchers, professors, and their students

Peptide Synthesis Protocols

Michael W. Pennington,Ben M. Dunn
Peptide Synthesis Protocols Book PDF

Author : Michael W. Pennington,Ben M. Dunn
Publisher : Humana Press
Page : 321 pages
File Size : 48,5 Mb
Release : 1994-11-07
Category : Science
ISBN : 0896032736

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Peptide Synthesis Protocols by Michael W. Pennington,Ben M. Dunn Pdf

Peptide synthesis has emerged as one of the most powerful tools in biochemical, pharmacological, immunological, and biophysical la- ratories. Recent improvements include general solid-phase method- ogy, new protecting groups, and automated equipment. These advances have allowed the facile synthesis of increasingly more complex p- tides. Many of these new and improved methods for the synthesis of peptides and peptide-related substances have been reported in various publications, but never compiled in a convenient handbook. Like other volumes in this series, Peptide Synthesis Protocols concentrates on the practical aspects of these procedures, providing the researcher with detailed descriptions and helpful tips about potential problems. This volume is not intended to serve as a basic guide to standard Merrifie- type solid-phase strategy, but rather to provide the researcher with some of the most recent applications in the field of peptide science. A c- panion volume, Peptide Analysis Protocols, will detail methodology for the charaterization of new synthetic peptides. Development of new methods and applications has continued actively even as this volume was in preparation. Owing to the number of contributors to this volume, it was necessary to establish a cutoff for publication purposes. We feel that all of the protocols presented are timely and up-to-date. Several promising new strategies, such as allyloxycarbonyl-based syntheses, were being developed at the time this volume was in the editing stages and will be included in future editions.

Amino Acid Analysis Protocols

Catherine Cooper
Amino Acid Analysis Protocols Book PDF

Author : Catherine Cooper
Publisher : Springer Science & Business Media
Page : 268 pages
File Size : 52,8 Mb
Release : 2008-02-05
Category : Science
ISBN : 9781592590476

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Amino Acid Analysis Protocols by Catherine Cooper Pdf

A collection of classic and cutting-edge techniques of high utility in answering specific biological questions about amino acids. Common methods include those based on HPLC or gas chromatography separation and analysis after precolumn derivatization. New techniques based on capillary electrophoresis separation, high-performance anion exchange chromatography, and mass spectrometry are also presented. Each method is described in step-by-step detail to ensure successful experimental results and emphasizes sample preparation, particularly the collection and storage of bodily fluids. Up-to-date and highly practical, Amino Acid Analysis Protocols offers analytical and clinical chemists, as well as a broad range of biological and biomedical investigators, a rich compendium of laboratory tools for the productive analysis of both common and uncommon amino acids.

Combinatorial Peptide Library Protocols

Shmuel Cabilly
Combinatorial Peptide Library Protocols Book PDF

Author : Shmuel Cabilly
Publisher : Springer Science & Business Media
Page : 320 pages
File Size : 45,8 Mb
Release : 2008-02-02
Category : Science
ISBN : 9781592595716

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Combinatorial Peptide Library Protocols by Shmuel Cabilly Pdf

During the course of evolution, an imbalance was created between the rate of vertebrate genetic adaptation and that of the lower forms of living organisms, such as bacteria and viruses. This imbalance has given the latter the advantage of generating, relatively quickly, molecules with unexpected structures and features that carry a threat to vertebrates. To compensate for their weakness, vertebrates have accelerated their own evolutionary processes, not at the level of whole organism, but in specialized cells containing the genes that code for antibody molecules or for T-cell receptors. That is, when an immediate requirement for molecules capable of specific interactions arose, nature has preferred to speed up the mode of Darwinian evolution in pref- ence to any other approach (such as the use of X-ray diffraction studies and computergraphic analysis). Recently, Darwinian rules have been adapted for test tube research, and the concept of selecting molecules having particular characteristics from r- dom pools has been realized in the form of various chemical and biological combinatorial libraries. While working with these libraries, we noticed the interesting fact that when combinatorial libraries of oligopeptides were allowed to interact with different selector proteins, only the actual binding sites of these proteins showed binding properties, whereas the rest of the p- tein surface seemed "inert. " This seemingly common feature of protein- having no extra potential binding sites--was probably selected during evolution in order to minimize nonspecific interactions with the surrounding milieu.

Mass Spectrometry Data Analysis in Proteomics

Rune Matthiesen
Mass Spectrometry Data Analysis in Proteomics Book PDF

Author : Rune Matthiesen
Publisher : Springer Science & Business Media
Page : 322 pages
File Size : 44,8 Mb
Release : 2008-02-02
Category : Science
ISBN : 9781597452755

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Mass Spectrometry Data Analysis in Proteomics by Rune Matthiesen Pdf

This is an in-depth guide to the theory and practice of analyzing raw mass spectrometry (MS) data in proteomics. The volume outlines available bioinformatics programs, algorithms, and databases available for MS data analysis. General guidelines for data analysis using search engines such as Mascot, Xtandem, and VEMS are provided, with specific attention to identifying poor quality data and optimizing search parameters.

2-D Proteome Analysis Protocols

Andrew J. Link
2 D Proteome Analysis Protocols Book PDF

Author : Andrew J. Link
Publisher : Springer Science & Business Media
Page : 585 pages
File Size : 49,8 Mb
Release : 2008-02-02
Category : Science
ISBN : 9781592595846

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2-D Proteome Analysis Protocols by Andrew J. Link Pdf

With the completion of sequencing projects and the advancement of a- lytical tools for protein identification, proteomics—the study of the expressed part of the genome—has become a major region of the burgeoning field of functional genomics. High-resolution 2-D gels can reveal virtually all p- teins present in a cell or tissue at any given time, including posttranslationally modified proteins. Changes in the expression and structure of most cellular proteins caused by differentiation or external stimuli can be displayed and eventually identified using 2-D protein gels. 2-D Proteome Analysis Protocols covers all aspects of the use of 2-D protein electrophoresis for the analysis of biological problems. The contri- tors include many of the leaders in the fields of biochemistry and analytical chemistry who were instrumental in the development of high-resolution 2-D gels, immobilized pH gradients, computer analysis, and mass spectromet- based protein identification methodologies. This book is intended as a benchtop manual and guide both for novices to 2-D gels and for those aficionados who wish to try the newer techniques. Any group using protein biochemistry—especially in the fields of molecular biology, biochemistry, microbiology, and cell biology—should find this book eminently useful. 2-D Proteome Analysis Protocols takes the researcher through the c- plete process of working with 2-D protein gels from making the protein - tract to finally identifying the proteins of interest. It includes protocols for generating 2-D protein extracts from most of the standard model organisms, including bacteria, yeast, nematode, Drosophila, plants, mouse, and human.

Peptide Nucleic Acids

Peter E. Nielsen
Peptide Nucleic Acids Book PDF

Author : Peter E. Nielsen
Publisher : Humana Press
Page : 274 pages
File Size : 47,8 Mb
Release : 2002-07-23
Category : Science
ISBN : 0896039765

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Peptide Nucleic Acids by Peter E. Nielsen Pdf

Peptide nucleic acids (PNAs) have now existed for slightly more than ten years, with the interest in and applications of this pseudopeptide DNA mimic steadily increasing during the entire period. PNAs have rapidly attracted the attention of scientists from a diversity of fields ranging from (bio)organic and biophysical chemistry to prebiotic evolution, and from molecular biology to genetic diagnostics and drug development. Many of the applications take advantage of the unique properties of PNA—an uncharged pseudopeptide—that distinguish this DNA mimic from more traditional DNA analogs. Rather than trying to create a comprehensive collection of all published methods and protocols involving PNA—many of which have not yet been validated— I have decided to concentrate on select protocols that are either very well established by several groups around the world, such as PCR-clamping and in situ hybridization, or on new methods that may have broader future impact. Basic methods for PNA oligomer synthesis and analyses have also been included. I am very grateful to those friends and colleagues who have enthusiastically contributed their work, discussions, and writing, and thereby made this book possible. Peter E. Nielsen v Contents Preface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v Contributors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ix IINTRODUCTION 1 PNA Technology Peter E. Nielsen. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 II CHEMISTRY 2 Solid Phase Synthesis of PNA Oligomers Frederik Beck. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29 3 Synthesis of PNA-Peptide Conjugates Satish Kumar Awasthi and Peter E. Nielsen. . . . . . . . . . . . . . . . . . 43 4 Parallel Synthesis of PNA-Peptide Conjugate Libraries Satish Kumar Awasthi and Peter E. Nielsen. . . . . . . . . . . . . . . . . .

Protein Purification Protocols

Paul Cutler
Protein Purification Protocols Book PDF

Author : Paul Cutler
Publisher : Springer Science & Business Media
Page : 474 pages
File Size : 45,8 Mb
Release : 2008-02-02
Category : Science
ISBN : 9781592596553

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Protein Purification Protocols by Paul Cutler Pdf

The first edition of Protein Purification Protocols (1996), edited by Professor Shawn Doonan, rapidly became very successful. Professor Doonan achieved his aims of p- ducing a list of protocols that were invaluable to newcomers in protein purification and of significant benefit to established practitioners. Each chapter was written by an ex- rienced expert in the field. In the intervening time, a number of advances have w- ranted a second edition. However, in attempting to encompass the recent developments in several areas, the intention has been to expand on the original format, retaining the concepts that made the initial edition so successful. This is reflected in the structure of this second edition. I am indebted to Professor Doonan for his involvement in this new edition and the continuity that this brings. Each chapter that appeared in the original volume has been reviewed and updated to reflect advances and bring the topic into the 21st century. In many cases, this reflects new applications or new matrices available from vendors. Many of these have increased the performance and/or scope of the given method. Several new chapters have been introduced, including chapters on all the currently used protein fractionation and ch- matographic techniques. They introduce the theory and background for each method, providing lists of the equipment and reagents required for their successful execution, as well as a detailed description of how each is performed.