Plant Tissue Culture And Transformation Techniques

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Plant Tissue Culture and Transformation Techniques

Balram Sharma
Plant Tissue Culture and Transformation Techniques Book PDF

Author : Balram Sharma
Publisher : Daya Books
Page : 178 pages
File Size : 50,8 Mb
Release : 2008
Category : Horticultural crops
ISBN : 8170355273

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Plant Tissue Culture and Transformation Techniques by Balram Sharma Pdf

This publication deals with various aspects of the genetic engineering-plant tissue culture and transformation techniques. Due to their biological, ecological and geographic diversity, the demand for various horticultural crops is likely to increase manifold in the future and in order to meet such demand, there is an urgent need to concentrate on the research aspects for improvement of these crops. Plant tissues culture offers new tools to accomplish this objective. Plant tissue culture is an important area of biotechnology, whic is used for the propagation of problem-species, rapid propagation of high value genotypes, production of secondary metabolites etc. Tissue culture is an important step in developing new hybrids from distant parents and transgenics and particularly cost-effective technology with palpable impact in vegetatively propagated plants, which is celarly visible in improved yields of cultivars incorporating genes from unexplored sources and improved germplasm, enhancement of quality parameters and supply of disease-free clones of true-to-type planting materials. Plant tissue culture is the most rapid and efficacious way to speedy productin of large volumes of identical plants for specific markets. Micropropagation is the quickest way for popularization of new varieties of horiticultural crops where other methods of mass multiplication of genetically pure and homogeneous planting materials are very slow. With the advent of transformation technology, it has become a useful tool to mass produce new plants with genetic material transferred from unrelated sources with the help of tissue culture. The volume contains contributions by several authors highlighting the status of genetic engineering and plant tissue culture research and development programmes in various developing countries and case studies on a few economically important crops. The publication will be of immense value to the working scientists, institutions, policy makers and all those bearing responsibility to develop, implement and intensify programmes in the related subjects in their respective countries. This book provides a good picture of efforts being made and success already achieved in the Third World countries at various levels of development striving to secure gains from the latest advances in science and technology. Contents Chapter 1: China-Cotton Genetic Engineering and Tissue Culture Developments by Reddy Naganagouda and Zhu Shuijin; Chapter 2: Egypt: Development of Transgenic Wheat with Improved Salt and Drought Tolerance by Ahmed Bahelidin & Hala F Eissa; Chapter 3: Egypt-Use of Genetic Engineering Approach to Develop Virus Resistance for Some Plants Belonging to Different Plant Families by Atef Shoukry Sadik; Chapter 4: Egypt-Genetic Transformation of Maize (Zea mays L) by Shireen Assem; Chapter 5: Egypt-Tissue Culture and Transformation of Potato by Taymour Nasr El Din; Chapter 6: Eritrea-Genetic Engineering by Tadesse Mehari; Chapter 7: India-Present Status, Policy and Constrains in Genetic Engineering by Jeetendra Jaysing Solanki; Chapter 8: Indonesia-Review on the Role of Biotechnology for Food Security by Lukit Devy; Chapter 9: Iran-Status of Agricultural Biotechnology by M Kafi; Chapter 10: Kenya-Status of Biotechnology Research and Development by C N Ngaman, M G Karembu and D Otunge; Chapter 11: Kenya-Present Status, Policies and Constraints in Areas Related to Plant Biotechnology by Salome Mallowa Obura; Chapter 12: Malaysia-A Brief Report on Biotechnology and Genetic Engineering by Z A Aziz; Chapter 13: Pakistan-Present Status, Policies and Constraints of Biotechnology by Saghir Ahmed Sheikh; Chapter 14: Sri Lanks-Present Status of Biotechnology by P Aruni Weerasinghe; Chapter 15: Syria-Current Status and Future Prospective of Agricultural Biotechnology Program at GCSAR by Nabila Ali Bacha; Chapter 16: Uganda-Report on the Present Status Policies and Constraints to Genetic Engineering by Kyeyune Gerald Muwanga.

Plant Tissue Culture Concepts and Laboratory Exercises

Robert N. Trigiano
Plant Tissue Culture Concepts and Laboratory Exercises Book PDF

Author : Robert N. Trigiano
Publisher : Routledge
Page : 470 pages
File Size : 51,6 Mb
Release : 2018-04-27
Category : Science
ISBN : 9781351424035

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Plant Tissue Culture Concepts and Laboratory Exercises by Robert N. Trigiano Pdf

Alternating between topic discussions and hands-on laboratory experiments that range from the in vitro flowering of roses to tissue culture of ferns, Plant Tissue Culture Concepts and Laboratory Exercises, Second Edition, addresses the most current principles and methods in plant tissue culture research. The editors use the expertise of some of the top researchers and educators in plant biotechnology to furnish students, instructors and researchers with a broad consideration of the field. Divided into eight major parts, the text covers everything from the history of plant tissue culture and basic methods to propagation techniques, crop improvement procedures, specialized applications and nutrition of callus cultures. New topic discussions and laboratory exercises in the Second Edition include ""Micropropagation of Dieffenbachia,"" ""Micropropagation and in vitro flowering of rose,"" ""Propagation from nonmeristematic tissue-organogenesis,"" ""Variation in culture"" and ""Tissue culture of ferns.""It is the book's extensive laboratory exercises that provide a hands-on approach in illustrating various topics of discussion, featuring step-by-step procedures, anticipated results, and a list of materials needed. What's more, editors Trigiano and Gray go beyond mere basic principles of plant tissue culture by including chapters on genetic transformation techniques, and photographic methods and statistical analysis of data. In all, Plant Tissue Culture Concepts and Laboratory Exercises, Second Edition, is a veritable harvest of information for the continued study and research in plant tissue culture science.

Plant Cell and Tissue Culture

Indra K. Vasil,Trevor A. Thorpe
Plant Cell and Tissue Culture Book PDF

Author : Indra K. Vasil,Trevor A. Thorpe
Publisher : Springer Science & Business Media
Page : 593 pages
File Size : 51,5 Mb
Release : 2013-03-09
Category : Science
ISBN : 9789401726818

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Plant Cell and Tissue Culture by Indra K. Vasil,Trevor A. Thorpe Pdf

Plant Cell and Tissue Culture gives an exhaustive account of plant cell culture and genetic transformation, including detailed chapters on all major field and plantation crops. Part A presents a comprehensive coverage of all necessary laboratory techniques for the initiation, nutrition, maintenance and storage of plant cell and tissue cultures, including discussions on these topics, as well as on morphogenesis and regeneration, meristem and shoot tip culture, plant protoplasts, mutant cell lines, variation in tissue cultures, isogenic lines, fertilization control, cryopreservation, transformation, and the production of secondary metabolites. Part B then proceeds into detail on the specific in vitro culture of specific crops, including cereals, legumes, vegetables, potatoes, other roots and tubers, oilseeds, temperate fruits, tropical fruits, plantation crops, forest trees and ornamentals. Plant Cell and Tissue Culture is, and is likely to remain, the laboratory manual of choice, as well as a source of inspiration and a guide to all workers in the field.

Plant Tissue Culture

Roberta H. Smith
Plant Tissue Culture Book PDF

Author : Roberta H. Smith
Publisher : Academic Press
Page : 206 pages
File Size : 44,9 Mb
Release : 2013
Category : Science
ISBN : 9780124159204

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Plant Tissue Culture by Roberta H. Smith Pdf

Plant Tissue Culture, Third Edition builds on the classroom tested, audience proven manual that has guided users through successful plant culturing A.tumefaciens mediated transformation, infusion technology, the latest information on media components and preparation, and regeneration and morphogenesis along with new exercises and diagrams provide current information and examples. The included experiments demonstrate major concepts and can be conducted with a variety of plant material that are readily available throughout the year. This book provides a diverse learning experience and is appropriate for both university students and plant scientists. Provides new exercises demonstrating tobacco leaf infiltration to observe transient expression of proteins and subcellular location of the protein, and information on development of a customized protocol for protoplast isolation for other experimental systems Includes detailed drawings that complement both introductions and experiments Guides reader from lab setup to supplies, stock solution and media preparation, explant selection and disinfestations, and experimental observations and measurement Provides the latest techniques and media information, including A. tumefaciens mediated transformation and infusion technology Fully updated literature

Plant Tissue Culture

B. N. Sathyanarayana,Dalia B. Varghese
Plant Tissue Culture Book PDF

Author : B. N. Sathyanarayana,Dalia B. Varghese
Publisher : I. K. International Pvt Ltd
Page : 299 pages
File Size : 46,6 Mb
Release : 2007
Category : Botany, Experimental
ISBN : 9788189866112

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Plant Tissue Culture by B. N. Sathyanarayana,Dalia B. Varghese Pdf

Plant Tissue Culture forms an integral basis of the present day biotechnology. Plant Tissue Culture: Practices and New Experimental Protocols is being brought out to fill the existing gap in the available literature on plant tissue culture, especially focusing on the aspects of practical procedures and protocols of tissue culture. This book contains important experimental techniques and gives guidance on carrying out hands-on experiences. It has been designed in a simple way, giving all the necessary procedures as a general guideline and also necessary tips to maneuver any problem encountered. These tips are based on the first hand experiences of the author while teaching and researching the techniques of plant tissue culture. A unique feature of this book is the inclusion of several techniques describing the actual protocols experimented and developed with different plant species by different scientists. A substantial number of original colored plates including fluorescence photographs standout the book. This pioneering work is valuable for the students who are looking for fresh outlook and search.

Plant Tissue Culture Manual

K. Lindsey
Plant Tissue Culture Manual Book PDF

Author : K. Lindsey
Publisher : Springer Science & Business Media
Page : 138 pages
File Size : 50,5 Mb
Release : 2012-12-06
Category : Science
ISBN : 9789400901810

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Plant Tissue Culture Manual by K. Lindsey Pdf

This manual comprises a range of techniques for research workers in the fields of cell and molecular biology, physiology, plant breeding and propagation, and genetic engineering.

Plant Tissue Culture

S.S. Bhojwani
Plant Tissue Culture Book PDF

Author : S.S. Bhojwani
Publisher : Elsevier
Page : 470 pages
File Size : 49,5 Mb
Release : 2012-12-02
Category : Science
ISBN : 9780444598479

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Plant Tissue Culture by S.S. Bhojwani Pdf

During the past decade, Plant Tissue Culture (PTC) has attracted considerable attention because of its vital role in plant biotechnology. PTC offers novel approaches to plant production, propagation, and preservation. Some in vitro techniques are being applied on a commercial scale while many others hold great potential. Consequently, the literature in this area has grown rapidly.This book deals with recent developments in plant tissue culture, and presents a critical assessment of the proven and potential applications of the various in vitro techniques, it also highlights current problems limiting the application of tissue culture, and projects the future lines of research in this field.

Plant Tissue Culture, Development, and Biotechnology

Robert N. Trigiano,Dennis J. Gray
Plant Tissue Culture Development and Biotechnology Book PDF

Author : Robert N. Trigiano,Dennis J. Gray
Publisher : CRC Press
Page : 608 pages
File Size : 51,5 Mb
Release : 2016-03-30
Category : Medical
ISBN : 9781439896143

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Plant Tissue Culture, Development, and Biotechnology by Robert N. Trigiano,Dennis J. Gray Pdf

Under the vast umbrella of Plant Sciences resides a plethora of highly specialized fields. Botanists, agronomists, horticulturists, geneticists, and physiologists each employ a different approach to the study of plants and each for a different end goal. Yet all will find themselves in the laboratory engaging in what can broadly be termed biotechnol

Plant Cell Culture

Michael R. Davey,Paul Anthony
Plant Cell Culture Book PDF

Author : Michael R. Davey,Paul Anthony
Publisher : John Wiley & Sons
Page : 358 pages
File Size : 47,6 Mb
Release : 2010-05-20
Category : Science
ISBN : 0470686510

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Plant Cell Culture by Michael R. Davey,Paul Anthony Pdf

The ability to culture cells is fundamental for mass propagation and as a baseline for the genetic manipulation of plant nuclei and organelles. The introduction to Plant Cell Culture: Essential Methods provides a general background to plant cell culture, including basic principles, technologies and laboratory practices that underpin the more detailed techniques described in subsequent chapters. Whilst each chapter provides a background to the topic area and methodology, a crucial aspect is the provision of detailed protocols with emphasis on trouble shooting, describing common problems and detailed advice for their avoidance. Plant Cell Culture: Essential Methods provides the reader with a concise overview of these techniques, including micropropagation, mutagenesis, cryopreservation, genetic and plastid transformation and somatic cell technologies. This book will be an essential addition to any plant science laboratory's bookshelf. Highlights the best and most up-to-date techniques for working on plant cell culture Explains clearly and precisely how to carry out selected techniques in addition to background information on the various approaches Chapters are written by leading international authorities in the field and cover both well-known and new, tried and tested, methods for working in plant cell culture An essential laboratory manual for students and early-career researchers.

Plant Cell Culture Protocols

Robert D. Hall
Plant Cell Culture Protocols Book PDF

Author : Robert D. Hall
Publisher : Springer Science & Business Media
Page : 422 pages
File Size : 42,6 Mb
Release : 2008-02-02
Category : Science
ISBN : 9781592595839

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Plant Cell Culture Protocols by Robert D. Hall Pdf

Robert Hall and a panel of expert researchers present a comprehensive collection of the most frequently used and broadly applicable techniques for plant cell and tissue culture. Readily reproducible and extensively annotated, the methods cover culture initiation, maintenance, manipulation, application, and long-term storage, with emphasis on techniques for genetic modification and micropropagation. Many of these protocols are currently used in major projects designed to produce improved varieties of important crop plants. Plant Cell Culture Protocols's state-of-the-art techniques are certain to make the book today's reference of choice, an indispensable tool in the development of new transgenic plants and full-scale commercial applications.

Plant Tissue Culture

Robert H. Smith
Plant Tissue Culture Book PDF

Author : Robert H. Smith
Publisher : Academic Press
Page : 190 pages
File Size : 44,5 Mb
Release : 2012-12-02
Category : Science
ISBN : 9780323160476

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Plant Tissue Culture by Robert H. Smith Pdf

Plant Tissue Culture Techniques and Experiments is a manual that contains laboratory exercises about the demonstration of the methods and different plant materials used in plant tissue culture. It provides an overview on the plant cell culture techniques and plant material options in selecting the explant source. This book starts by discussing the proper setup of a tissue culture laboratory and the selection of the culture medium. It then explains the determination of an explant which is the ultimate goal of the cell culture project. The explant is a piece of plant tissue that is used in tissue culture. Furthermore, the book discusses topics about callus induction, regeneration and morphogenesis process, and haploid plants from anther and pollen culture. The meristem culture for virus-free plants and in vitro propagation for commercial propagation of ornamentals are also explained in this manual. The book also provides topics and exercises on the protoplast isolation and fusion and agrobacterium-mediated transformation of plants. This manual is intended for college students, both graduate and undergraduate, who study chemistry, plant anatomy, and plant physiology.

Plant Cell and Tissue Culture – A Tool in Biotechnology

Karl-Hermann Neumann,Ashwani Kumar,Jafargholi Imani
Plant Cell and Tissue Culture A Tool in Biotechnology Book PDF

Author : Karl-Hermann Neumann,Ashwani Kumar,Jafargholi Imani
Publisher : Springer Nature
Page : 459 pages
File Size : 45,9 Mb
Release : 2020-10-01
Category : Science
ISBN : 9783030490980

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Plant Cell and Tissue Culture – A Tool in Biotechnology by Karl-Hermann Neumann,Ashwani Kumar,Jafargholi Imani Pdf

This textbook is clearly structured with fourteen richly illustrated chapters and practical examples for easy understanding and direct implementation. The methods and findings developed in the authors’ group are presented in detailed, revised chapters. Readers will find valuable updates on the molecular basis of biotechnological processes, secondary metabolite production and genetic engineering. In addition, the basic principles of important biotechnologies, as well as examples of specially designed crops that deliver improved productivity under stress conditions, are presented. This second edition sets the direction for future research on the basic aspects of plant tissue culture and its applications in the fields of secondary metabolite production and genetic engineering. It provides both general and specific information for students, teachers, academic researchers and industrial teams who are interested in new developments in plant tissue culture and its applications.

Thin Cell Layer Culture System: Regeneration and Transformation Applications

Duong Tan Nhut,Kiem Tran Thanh Van,B. Van Le,Trevor A. Thorpe
Thin Cell Layer Culture System Regeneration and Transformation Applications Book PDF

Author : Duong Tan Nhut,Kiem Tran Thanh Van,B. Van Le,Trevor A. Thorpe
Publisher : Springer Science & Business Media
Page : 522 pages
File Size : 45,6 Mb
Release : 2013-03-09
Category : Science
ISBN : 9789401735223

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Thin Cell Layer Culture System: Regeneration and Transformation Applications by Duong Tan Nhut,Kiem Tran Thanh Van,B. Van Le,Trevor A. Thorpe Pdf

Scientists within the field of plant biotechnology are in a constant search for techniques that can, in the simplest manner possible, answer the genetic and biochemical questions that underlie developmental processes. Thin Cell Layer Culture System not only takes an in-depth look at a technique that has had so much success in attempting, through various practical models and systems, to answer these issues, but also represents a celebration of almost 30 years of research that has covered a massive scope of plant species and areas of study. The initial studies conducted on tobacco thin cell layers (TCLs) - proving that organogenesis can be strictly controlled in vitro - allowed plant research to benefit from this finding, expanding this knowledge in a practical and applied manner into the biotechnological fields of tissue culture and micropropagation, cell and organ genetics and biochemistry. The chapters in this book tell the enigmatic tale of TCLs. An historical perspective opens the scene for an inquiry into the possible cellular, biochemical and genetic processes that allow for the controlled development of a TCL into any organ type. The success of the system is further demonstrated in both monocotyledonous and dicotyledonous species, covering successful organogenesis and in vitro flowering in species within ornamental, leguminous and wood crops, cereals and grasses. Methodologies are outlined in detail, as is the rationale behind the TCL-organogenesis-developmental sequel. The TCL method, shown to be superior to many conventional micropropagation systems, has also shown to be vital in the recovery of transgenic plants. This book is an essential part of every plant, cell and developmental biologist, geneticist and tissue culturalist's shelf as it addresses the primary issue of any plant: the cell, the tissue, and their subsequent development into a highly organized system.

Introduction to Plant Tissue Culture

M. K. Razdan
Introduction to Plant Tissue Culture Book PDF

Author : M. K. Razdan
Publisher : Science Publishers
Page : 394 pages
File Size : 44,8 Mb
Release : 2003
Category : Science
ISBN : 1578082374

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Introduction to Plant Tissue Culture by M. K. Razdan Pdf

Introduction and techniques; Introductory history; Laboratory organisation; Media; Aseptic manipulation; Basic aspects; Cell culture; Cellular totipotency; Somatic embryogenesis; Applications to plant breeding; Haploid prodution; Triploid production; In vitro pollination and fertilization; Zygotic embryo culture; Somatic hybridisation and cybridisation; Genetic transformation; Somaclonal and gametoclonal variant selection; Application to horticulture and forestry; Production of disease-free plants; clonal propagation; General applications; Industrial applications: secondary metabolite production; Germplasm conservation.

Plant Tissue Culture

Jayarama Reddy
Plant Tissue Culture Book PDF

Author : Jayarama Reddy
Publisher : CRC Press
Page : 226 pages
File Size : 42,6 Mb
Release : 2024-02-25
Category : Science
ISBN : 9781003862130

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Plant Tissue Culture by Jayarama Reddy Pdf

This book is a comprehensive text on plant tissue culture, with its past, present, and future prospects and techniques discussed in detail. In the first three chapters, the history, terminologies, and applications are given in detail. The fourth chapter is dedicated to the instrumentation of plant tissue culture. The basic techniques used in PTC are described in the sixth chapter. The details of the constituents and types of different nutrient media are discussed in the eighth chapter. In chapter number 9, methods of haploid production have been described. Bioreactors are the instruments that are used for the large-scale production of plantlets and plant products. This book is useful for all the students, researchers, teachers, and industrialists interested in plant tissue culture. Print edition not for sale in india. This book is a comprehensive text on plant tissue culture, with its past, present, and future prospects and techniques discussed in detail.