Practice Of High Performance Liquid Chromatography

Author : W.J. Lough,I.W. Wainer
Publisher : CRC Press
Page : 292 pages
File Size : 40,8 Mb
Release : 1995-09-30
Category : Science
ISBN : 0751400769

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High Performance Liquid Chromatography by W.J. Lough,I.W. Wainer Pdf

High performance liquid chromatography (HPLC) has long been recognized as one of the most useful and versatile analytical techniques. It has now progressed from being a highly expensive method of analysis to a routine technique with wide applications. Consequently there is a requirement in many chemistry and chemistry-related courses for students to acquire a detailed understanding of the principles and practice of HPLC. Written in a manner suitable for undergraduate students studying analytical chemistry and learning about chromatographic analytical techniques applied to pharmaceutical analysis, biochemistry and related disciplines, High-performance Liquid Chromatography: Fundamental Principles and Practice introduces the fundamentals of HPLC. Loosely structured in three parts, the text begins with a thorough introduction of the subject and then progresses through the essential knowledge of the instrumentation needed for HPLC. The final part covers with the applications of HPLC in real-world situations. Developed by a team of international experts from a wide cross-section of disciplines, the text is relevant to a wide range of courses.

Author : Heinz Engelhardt
Publisher : Springer Science & Business Media
Page : 468 pages
File Size : 49,8 Mb
Release : 2012-12-06
Category : Science
ISBN : 9783642692253

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Practice of High Performance Liquid Chromatography by Heinz Engelhardt Pdf

During its short 20 year history High Performance Liquid Chro matography (HPLC) has won itself a firm place amongst the instrumental methods of analysis. HPLC has caused a revolution in biological and pharmaceutical chemistry. Approximately two thirds of the publications on HPLC are concerned with problems from this area of life science. Biotechnology, where it is necessary to isolate substances from complicated mixtures, is likely to give further impetus to the dissemination of modern liquid chromatog raphy in columns, particularly on the preparative scale. This book presents, by means of examples, the application of HPLC to various fields, as well as fundamental discussions of chromatographic methods. The quality of the analytical result is decisively dependent on the qualities of the equipment employed (by Colin, Guiochon, and Martin). Especially the demands are discussed that are placed on the components of the instrument including those for data acquisition and processing. The section on "quantitative analy sis" (by ABhauer, Ullner) covers besides the principles also the problems of ensuring the quality of the data in detail. The basic problems arising by enlarging the sample size to preparative di mensions and the requirements put on the aparatus are discussed in the section on "preparative applications" (by Wehrli).

Author : Yuegang Zuo
Publisher : Nova Science Publishers
Page : 0 pages
File Size : 43,9 Mb
Release : 2014
Category : Chromatographic analysis
ISBN : 1629488542

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High-performance Liquid Chromatography (HPLC) by Yuegang Zuo Pdf

Includes bibliographical references and index.

Author : Mary T. Gilbert
Publisher : Elsevier
Page : 397 pages
File Size : 44,7 Mb
Release : 2013-10-22
Category : Science
ISBN : 9781483281292

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High Performance Liquid Chromatography by Mary T. Gilbert Pdf

High Performance Liquid Chromatography focuses on the developments, operating techniques, practices, equipment, and packing materials involved in High Performance Liquid Chromatography (HPLC). The book first offers information on basic chromatographic theory, equipment, and the column. Topics include resolution, efficiency, pumps and gradient systems, connectors, detectors, injectors, column packing and testing, packing materials, and coupling of columns. The text also ponders on sample treatment and separation methods, as well as trace analysis, reversed phase chromatography, and selection/optimization conditions. The publication examines adjustment of selectivity by the use of eluent additives and preparative liquid chromatography. Discussions focus on chromatography on dynamically modified oxide gels, metal complexation, crown ethers, ion pair chromatography, materials for preparative chromatography, and separation strategy. The text also reviews the trends in the practice of HPLC and chiral chromatography. The book is a dependable reference for readers interested in High Performance Liquid Chromatography.

Author : Sandie Lindsay
Publisher : John Wiley & Sons
Page : 368 pages
File Size : 40,6 Mb
Release : 1992-07-27
Category : Science
ISBN : 0471931152

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High Performance Liquid Chromatography by Sandie Lindsay Pdf

High performance liquid chromatography is the most powerful of all the chromatographic techniques, often achieving separations and analyses that would be difficult or impossible with other forms of chromatography. This study and training text examines the concepts and techniques used in this field. A selection of literature available from equipment manufacturers is included along with a brief review of some more specialized topics.

Author : Veronika R. Meyer
Publisher : John Wiley & Sons
Page : 434 pages
File Size : 45,7 Mb
Release : 2010-05-17
Category : Science
ISBN : 9780470682180

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Practical High-Performance Liquid Chromatography by Veronika R. Meyer Pdf

Jump into the HPLC adventure! Three decades on from publication of the 1st German edition of Veronika Meyer's book on HPLC, this classic text remains one of the few titles available on general HPLC aimed at practitioners. New sections on the following topics have been included in this fifth edition: Comparison of HPLC with capillary electrophoresis How to obtain peak capacity van Deemter curves and other coherences Hydrophilic interaction chromatography Method transfer Comprehensive two-dimensional HPLC Fast separations at 1000 bar HPLC with superheated water In addition, two chapters on the instrument test and troubleshooting in the appendix have been updated and expanded by Bruno E. Lendi, and many details have been improved and numerous references added. A completely new chapter is presented on quality assurance covering: Is it worth the effort? Verification with a second method Method validation Standard operating procedures Measurement uncertainty Qualifications, instrument test, and system suitability test The quest for quality Reviews of earlier editions "That this text is written by an expert in both the practice and teaching of HPLC is evident from the first paragraph....not only an enjoyable, fascinating and easy read, but a truly excellent text that has and will serve many teachers, students and practitioners very well." —The Analyst “…provides essential information on HPLC for LC practitioners in academia, industry, government, and research laboratories…a valuable introduction." - American Journal of Therapeutics

Author : S. Pryde,M.T. Gilbert
Publisher : Springer Science & Business Media
Page : 288 pages
File Size : 55,7 Mb
Release : 1979-01-31
Category : Science
ISBN : 0412142201

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Applications of High Performance Liquid Chromatography by S. Pryde,M.T. Gilbert Pdf

Author : Win Fung Ho,Brian Stuart,Elizabeth R. Prichard
Publisher : Royal Society of Chemistry
Page : 52 pages
File Size : 43,6 Mb
Release : 2003
Category : Education
ISBN : 0854044833

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High Performance Liquid Chromatography by Win Fung Ho,Brian Stuart,Elizabeth R. Prichard Pdf

"This book covers the basic practical aspects of high performance liquid chromatography (HPLC) and is aimed at the inexperienced analyst who may have no or very little knowledge of this technique. It includes basic tips, identifies key skills, arouses awareness and gives guidance on good practice of the basic aspects of HPLC." - page 1.

Author : Heinz Engelhardt
Publisher : Springer
Page : 274 pages
File Size : 45,9 Mb
Release : 1979
Category : Science
ISBN : UOM:39015016088307

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High Performance Liquid Chromatography by Heinz Engelhardt Pdf

Author : Andrea Weston,Phyllis R. Brown
Publisher : Elsevier
Page : 280 pages
File Size : 42,5 Mb
Release : 1997-07-21
Category : Science
ISBN : 0080534163

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High Performance Liquid Chromatography & Capillary Electrophoresis by Andrea Weston,Phyllis R. Brown Pdf

HPLC and CE: Principles and Practice presents the latest information on the most powerful separation techniques available: high-performance liquid chromatography (HPLC) and capillary electrophoresis (CE). Fundamental theory, instrumentation, modes of operation, and optimization of separations are presented in a concise, non-technical style to help the user in choosing the appropriate technique quickly and accurately. Well- illustrated and containing convenientend-of-chapter summaries of the major concepts, the book provides in-depth coverage of trouble-shooting, improvement of resolution, data manipulation, selectivity, and sensitivity. Graduate students, technicians, and researchers who must use separations with little or no background in analytical chemistry can overcome separation anxiety and get started in obtaining the best possible separations in minimal time. The book will alsobe useful to analytical chemists who need a better understanding of theory and processes. Fully up-to-date information on both HPLC and CE includes troubleshooting and comparisons of the two techniques Applicable to a wide variety of separation problems Covers basic concepts governing any separation as well as instrumentation and how to use it Helps the user to obtain optimal resolution in minimal time Contains information on special procedures such as chiral separations, affinity chromatography, and sample preparation Includes information on upcoming trends such as miniaturization Major concepts in each chapter are organized to allow access to information easily and quickly Contains practical bibliography for accessing the literature

Author : Michael W. Dong
Publisher : John Wiley & Sons
Page : 328 pages
File Size : 49,8 Mb
Release : 2016-04-06
Category : Science
ISBN : 9781119293606

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Modern HPLC for Practicing Scientists by Michael W. Dong Pdf

A comprehesive yet concise guide to Modern HPLC Written for practitioners by a practitioner, Modern HPLC for Practicing Scientists is a concise text which presents the most important High-Performance Liquid Chromatography (HPLC) fundamentals, applications, and developments. It describes basic theory and terminology for the novice, and reviews relevant concepts, best practices, and modern trends for the experienced practitioner. Moreover, the book serves well as an updated reference guide for busy laboratory analysts and researchers. Topics covered include: HPLC operation Method development Maintenance and troubleshooting Modern trends in HPLC such as quick-turnaround and "greener" methods Regulatory aspects While broad in scope, this book focuses particularly on reversed-phase HPLC, the most common separation mode, and on applications for the pharmaceutical industry, the largest user segment. Accessible to both novice and intermedate HPLC users, information is delivered in a straightforward manner illustrated with an abundance of diagrams, chromatograms, tables, and case studies, and supported with selected key references and Web resources. With intuitive explanations and clear figures, Modern HPLC for Practicing Scientists is an essential resource for practitioners of all levels who need to understand and utilize this versatile analytical technology.

Author : Veronika Meyer
Publisher : Unknown
Page : 360 pages
File Size : 50,6 Mb
Release : 1998
Category : Science
ISBN : UOM:39015043765034

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Practical High-Performance Liquid Chromatography by Veronika Meyer Pdf

Practical High-Performance Liquid Chromatography Third Edition Veronika R. Meyer Swiss Federal Laboratories for Materials Testing and Research, St. Gallen, Switzerland Veronika Meyer's book is a classic HPLC text and remains one of the few titles on general HPLC. Following on from the excellent success of the first two editions, this third edition continues to provide postgraduate students using HPLC and users of HPLC in industry/pharmaceuticals with a unified approach to HPLC and an equal treatment of the theory and practice of HPLC. This edition provides numerous additions and updated material, including: * 10 out of 26 chapters substantially revised * Synopsis of the most important formulas * lnclusion of chapters on solvent properties and on instrument tests * Completely rewritten chapter on pumps * Updated figures and references

Author : Lloyd R. Snyder,Joseph J. Kirkland,John W. Dolan
Publisher : John Wiley & Sons
Page : 831 pages
File Size : 46,9 Mb
Release : 2011-09-20
Category : Science
ISBN : 9781118210390

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Introduction to Modern Liquid Chromatography by Lloyd R. Snyder,Joseph J. Kirkland,John W. Dolan Pdf

The latest edition of the authoritative reference to HPLC High-performance liquid chromatography (HPLC) is today the leading technique for chemical analysis and related applications, with an ability to separate, analyze, and/or purify virtually any sample. Snyder and Kirkland's Introduction to Modern Liquid Chromatography has long represented the premier reference to HPLC. This Third Edition, with John Dolan as added coauthor, addresses important improvements in columns and equipment, as well as major advances in our understanding of HPLC separation, our ability to solve problems that were troublesome in the past, and the application of HPLC for new kinds of samples. This carefully considered Third Edition maintains the strengths of the previous edition while significantly modifying its organization in light of recent research and experience. The text begins by introducing the reader to HPLC, its use in relation to other modern separation techniques, and its history, then leads into such specific topics as: The basis of HPLC separation and the general effects of different experimental conditions Equipment and detection The column—the "heart" of the HPLC system Reversed-phase separation, normal-phase chromatography, gradient elution, two-dimensional separation, and other techniques Computer simulation, qualitative and quantitative analysis, and method validation and quality control The separation of large molecules, including both biological and synthetic polymers Chiral separations, preparative separations, and sample preparation Systematic development of HPLC separations—new to this edition Troubleshooting tricks, techniques, and case studies for both equipment and chromatograms Designed to fulfill the needs of the full range of HPLC users, from novices to experts, Introduction to Modern Liquid Chromatography, Third Edition offers the most up-to-date, comprehensive, and accessible survey of HPLC methods and applications available.

Author : Serban C. Moldoveanu,Victor David
Publisher : Elsevier
Page : 598 pages
File Size : 53,6 Mb
Release : 2016-11-01
Category : Science
ISBN : 9780128037119

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Selection of the HPLC Method in Chemical Analysis by Serban C. Moldoveanu,Victor David Pdf

Selection of the HPLC Method in Chemical Analysis serves as a practical guide to users of high-performance liquid chromatography and provides criteria for method selection, development, and validation. High-performance liquid chromatography (HPLC) is the most common analytical technique currently practiced in chemistry. However, the process of finding the appropriate information for a particular analytical project requires significant effort and pre-existent knowledge in the field. Further, sorting through the wealth of published data and literature takes both time and effort away from the critical aspects of HPLC method selection. For the first time, a systematic approach for sorting through the available information and reviewing critically the up-to-date progress in HPLC for selecting a specific analysis is available in a single book. Selection of the HPLC Method in Chemical Analysis is an inclusive go-to reference for HPLC method selection, development, and validation. Addresses the various aspects of practice and instrumentation needed to obtain reliable HPLC analysis results Leads researchers to the best choice of an HPLC method from the overabundance of information existent in the field Provides criteria for HPLC method selection, development, and validation Authored by world-renowned HPLC experts who have more than 60 years of combined experience in the field

Author : Lloyd R. Snyder,Joseph J. Kirkland,Joseph L. Glajch
Publisher : John Wiley & Sons
Page : 665 pages
File Size : 49,6 Mb
Release : 2012-12-03
Category : Science
ISBN : 9781118591512

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Practical HPLC Method Development by Lloyd R. Snyder,Joseph J. Kirkland,Joseph L. Glajch Pdf

This revision brings the reader completely up to date on the evolving methods associated with increasingly more complex sample types analyzed using high-performance liquid chromatography, or HPLC. The book also incorporates updated discussions of many of the fundamental components of HPLC systems and practical issues associated with the use of this analytical method. This edition includes new or expanded treatments of sample preparation, computer assisted method development, as well as biochemical samples, and chiral separations.