Applications Of High Performance Liquid Chromatography

Author : Q. Alan Xu
Publisher : John Wiley & Sons
Page : 342 pages
File Size : 46,8 Mb
Release : 2013-04-01
Category : Technology & Engineering
ISBN : 9781118533987

Get Book

Ultra-High Performance Liquid Chromatography and Its Applications by Q. Alan Xu Pdf

Explores both the benefits and limitations of new UHPLC technology High performance liquid chromatography (HPLC) has been widely used in analytical chemistry and biochemistry to separate, identify, and quantify compounds for decades. The science of liquid chromatography, however, was revolutionized a few years ago with the advent of ultra-high performance liquid chromatography (UHPLC), which made it possible for researchers to analyze sample compounds with greater speed, resolution, and sensitivity. Ultra-High Performance Liquid Chromatography and Its Applications enables readers to maximize the performance of UHPLC as well as develop UHPLC methods tailored to their particular research needs. Readers familiar with HPLC methods will learn how to transfer these methods to a UHPLC platform and vice versa. In addition, the book explores a variety of UHPLC applications designed to support research in such fields as pharmaceuticals, food safety, clinical medicine, and environmental science. The book begins with discussions of UHPLC method development and method transfer between HPLC and UHPLC platforms. It then examines practical aspects of UHPLC. Next, the book covers: Coupling UHPLC with mass spectrometry Potential of shell particles in fast liquid chromatography Determination of abused drugs in human biological matrices Analyses of isoflavones and flavonoids Therapeutic protein characterization Analysis of illicit drugs The final chapter of the book explores the use of UHPLC in drug metabolism and pharmacokinetics studies for traditional Chinese medicine. With its frank discussions of UHPLC's benefits and limitations, Ultra-High Performance Liquid Chromatography and Its Applications equips analytical scientists with the skills and knowledge needed to take full advantage of this new separation technology.

Author : Shirley Bayne,Michelle Carlin
Publisher : CRC Press
Page : 276 pages
File Size : 50,6 Mb
Release : 2017-07-27
Category : Law
ISBN : 9781439882597

Get Book

Forensic Applications of High Performance Liquid Chromatography by Shirley Bayne,Michelle Carlin Pdf

Chromatography has many roles in forensic science, ranging from toxicology to environmental analysis. In particular, high-performance liquid chromatography (HPLC) is a primary method of analysis in many types of laboratories. Maintaining a balance between practical solutions and the theoretical considerations involved in HPLC analysis, Forensic App

Author : Heinz Engelhardt
Publisher : Springer Science & Business Media
Page : 468 pages
File Size : 45,6 Mb
Release : 2012-12-06
Category : Science
ISBN : 9783642692253

Get Book

Practice of High Performance Liquid Chromatography by Heinz Engelhardt Pdf

During its short 20 year history High Performance Liquid Chro matography (HPLC) has won itself a firm place amongst the instrumental methods of analysis. HPLC has caused a revolution in biological and pharmaceutical chemistry. Approximately two thirds of the publications on HPLC are concerned with problems from this area of life science. Biotechnology, where it is necessary to isolate substances from complicated mixtures, is likely to give further impetus to the dissemination of modern liquid chromatog raphy in columns, particularly on the preparative scale. This book presents, by means of examples, the application of HPLC to various fields, as well as fundamental discussions of chromatographic methods. The quality of the analytical result is decisively dependent on the qualities of the equipment employed (by Colin, Guiochon, and Martin). Especially the demands are discussed that are placed on the components of the instrument including those for data acquisition and processing. The section on "quantitative analy sis" (by ABhauer, Ullner) covers besides the principles also the problems of ensuring the quality of the data in detail. The basic problems arising by enlarging the sample size to preparative di mensions and the requirements put on the aparatus are discussed in the section on "preparative applications" (by Wehrli).

Author : W.J. Lough,I.W. Wainer
Publisher : CRC Press
Page : 292 pages
File Size : 41,5 Mb
Release : 1995-09-30
Category : Science
ISBN : 0751400769

Get Book

High Performance Liquid Chromatography by W.J. Lough,I.W. Wainer Pdf

High performance liquid chromatography (HPLC) has long been recognized as one of the most useful and versatile analytical techniques. It has now progressed from being a highly expensive method of analysis to a routine technique with wide applications. Consequently there is a requirement in many chemistry and chemistry-related courses for students to acquire a detailed understanding of the principles and practice of HPLC. Written in a manner suitable for undergraduate students studying analytical chemistry and learning about chromatographic analytical techniques applied to pharmaceutical analysis, biochemistry and related disciplines, High-performance Liquid Chromatography: Fundamental Principles and Practice introduces the fundamentals of HPLC. Loosely structured in three parts, the text begins with a thorough introduction of the subject and then progresses through the essential knowledge of the instrumentation needed for HPLC. The final part covers with the applications of HPLC in real-world situations. Developed by a team of international experts from a wide cross-section of disciplines, the text is relevant to a wide range of courses.

Author : S. Pryde,M.T. Gilbert
Publisher : Springer Science & Business Media
Page : 288 pages
File Size : 51,9 Mb
Release : 1979-01-31
Category : Science
ISBN : 0412142201

Get Book

Applications of High Performance Liquid Chromatography by S. Pryde,M.T. Gilbert Pdf

Author : Sandie Lindsay
Publisher : John Wiley & Sons
Page : 368 pages
File Size : 42,6 Mb
Release : 1992-07-27
Category : Science
ISBN : 0471931152

Get Book

High Performance Liquid Chromatography by Sandie Lindsay Pdf

High performance liquid chromatography is the most powerful of all the chromatographic techniques, often achieving separations and analyses that would be difficult or impossible with other forms of chromatography. This study and training text examines the concepts and techniques used in this field. A selection of literature available from equipment manufacturers is included along with a brief review of some more specialized topics.

Author : R. J. Hamilton
Publisher : Unknown
Page : 0 pages
File Size : 47,8 Mb
Release : 1982-06-03
Category : Electronic
ISBN : 9400959397

Get Book

Introduction to High Performance Liquid Chromatography by R. J. Hamilton Pdf

Author : Mary T. Gilbert
Publisher : Elsevier
Page : 396 pages
File Size : 49,6 Mb
Release : 2013-10-22
Category : Science
ISBN : 9781483281292

Get Book

High Performance Liquid Chromatography by Mary T. Gilbert Pdf

High Performance Liquid Chromatography focuses on the developments, operating techniques, practices, equipment, and packing materials involved in High Performance Liquid Chromatography (HPLC). The book first offers information on basic chromatographic theory, equipment, and the column. Topics include resolution, efficiency, pumps and gradient systems, connectors, detectors, injectors, column packing and testing, packing materials, and coupling of columns. The text also ponders on sample treatment and separation methods, as well as trace analysis, reversed phase chromatography, and selection/optimization conditions. The publication examines adjustment of selectivity by the use of eluent additives and preparative liquid chromatography. Discussions focus on chromatography on dynamically modified oxide gels, metal complexation, crown ethers, ion pair chromatography, materials for preparative chromatography, and separation strategy. The text also reviews the trends in the practice of HPLC and chiral chromatography. The book is a dependable reference for readers interested in High Performance Liquid Chromatography.

Author : A. Fallon,R.F.G. Booth,L.D. Bell
Publisher : Elsevier
Page : 337 pages
File Size : 51,8 Mb
Release : 1987-07-01
Category : Science
ISBN : 0080858864

Get Book

Applications of HPLC in Biochemistry by A. Fallon,R.F.G. Booth,L.D. Bell Pdf

This book is intended to familiarize biochemists with HPLC. Theoretical aspects of each mode of chromatography are discussed in chapters 1-9, providing an understanding of the various modes of chromatography which are now possible using commercially available columns, from reversed phase to affinity. Practical aspects and instrumentation are covered in chapter 10. The bulk of the book, which follows, presents examples and applications of each mode of chromatography in current biochemical practice.

Author : Omar Al Sayed Omar,Moustafa A. Khalifa
Publisher : Walter de Gruyter GmbH & Co KG
Page : 322 pages
File Size : 55,6 Mb
Release : 2022-02-21
Category : Science
ISBN : 9783110529456

Get Book

High Performance Liquid Chromatography by Omar Al Sayed Omar,Moustafa A. Khalifa Pdf

The book provides an indispensable guide on how to use HPLC in pharmaceutical analysis and drug control. Following a hands-on approach, the authors give practical advices how to prepare stationary and mobile phases, choose a suitable detector and set up an HPLC analysis. The publication gives insight into the key pharmaceutical applications of HPLC and the latest requirements of the major regulatory agencies.

Author : Yuegang Zuo
Publisher : Nova Science Publishers
Page : 0 pages
File Size : 48,6 Mb
Release : 2014
Category : Chromatographic analysis
ISBN : 1629488542

Get Book

High-performance Liquid Chromatography (HPLC) by Yuegang Zuo Pdf

Includes bibliographical references and index.

Author : Colin T. Mant,Robert S. Hodges
Publisher : CRC Press
Page : 1250 pages
File Size : 45,5 Mb
Release : 2017-11-22
Category : Medical
ISBN : 9781351440745

Get Book

High-Performance Liquid Chromatography of Peptides and Proteins by Colin T. Mant,Robert S. Hodges Pdf

This book consists of a series of 82 precise, easy-to-read articles by internationally renowned scientists and emphasizes the practical approach to HPLC with minimal theory, although the underlying principles for peptide and protein separations are clearly expressed. All of the major modes of microbore, ultrafast and analytical HPLC are discussed, including size-exclusion, ion-exchange, reversed-phase, hydrophobic interaction, and affinity and immunoaffinity chromatography. A section on preparative HPLC, including displacement techniques, is also presented. Problem-solving approaches to the separation of various classes of biologically active peptides and proteins are thoroughly explored, while the importance of peptide standards for monitoring column performance and for optimizing separation conditions is emphasized. Several articles focus on the choice of the correct detection method (electrochemical, UV, fluorescence), as well as the need for a proper knowledge of approaches to column and instrument maintenance and trouble-shooting. A section on predictive approaches deals with both computer simulation of peptide separations and peptide structure. The book also includes complementary techniques to HPLC, as well as other useful applications of HPLC. It enables both novice and experienced chromatographers to realize the full potential of this extremely powerful technique, in the process making an important contribution to scientific literature.

Author : Joel K. Swadesh
Publisher : CRC Press
Page : 480 pages
File Size : 45,7 Mb
Release : 2000-12-21
Category : Science
ISBN : 9781420042665

Get Book

HPLC by Joel K. Swadesh Pdf

Product specifications, regulatory constraints, and tight production schedules impose considerable pressures on separation scientists in industry. The first edition of HPLC: Practical and Industrial Applications helped eliminate the need for extensive library or laboratory research when confronting a problem, an unfamiliar technique, or work in a n

Author : Csaba Horváth
Publisher : Elsevier
Page : 342 pages
File Size : 45,6 Mb
Release : 2013-10-22
Category : Science
ISBN : 9781483262024

Get Book

High-Performance Liquid Chromatography by Csaba Horváth Pdf

High-Performance Liquid Chromatography: Advances and Perspectives, Volume 1 deals with the fundamental aspects of high-performance liquid chromatography, a technique used in chemical analysis. The publication provides accounts, presented by experts in the field, of a variety of topics in high-performance liquid chromatography. Each chapter covers interesting subjects such as the evolution of liquid chromatography; the use of bonded phases in high-performance chromatography; effects of ionization and complex formation on retention and selectivity in reversed-phase chromatography; and gradient elution. Chromatographers, chemists, and researchers in the field of chemical analysis will find this book a valuable reference material.

Author : Lloyd R. Snyder,Joseph J. Kirkland,John W. Dolan
Publisher : John Wiley & Sons
Page : 831 pages
File Size : 51,8 Mb
Release : 2011-09-20
Category : Science
ISBN : 9781118210390

Get Book

Introduction to Modern Liquid Chromatography by Lloyd R. Snyder,Joseph J. Kirkland,John W. Dolan Pdf

The latest edition of the authoritative reference to HPLC High-performance liquid chromatography (HPLC) is today the leading technique for chemical analysis and related applications, with an ability to separate, analyze, and/or purify virtually any sample. Snyder and Kirkland's Introduction to Modern Liquid Chromatography has long represented the premier reference to HPLC. This Third Edition, with John Dolan as added coauthor, addresses important improvements in columns and equipment, as well as major advances in our understanding of HPLC separation, our ability to solve problems that were troublesome in the past, and the application of HPLC for new kinds of samples. This carefully considered Third Edition maintains the strengths of the previous edition while significantly modifying its organization in light of recent research and experience. The text begins by introducing the reader to HPLC, its use in relation to other modern separation techniques, and its history, then leads into such specific topics as: The basis of HPLC separation and the general effects of different experimental conditions Equipment and detection The column—the "heart" of the HPLC system Reversed-phase separation, normal-phase chromatography, gradient elution, two-dimensional separation, and other techniques Computer simulation, qualitative and quantitative analysis, and method validation and quality control The separation of large molecules, including both biological and synthetic polymers Chiral separations, preparative separations, and sample preparation Systematic development of HPLC separations—new to this edition Troubleshooting tricks, techniques, and case studies for both equipment and chromatograms Designed to fulfill the needs of the full range of HPLC users, from novices to experts, Introduction to Modern Liquid Chromatography, Third Edition offers the most up-to-date, comprehensive, and accessible survey of HPLC methods and applications available.