Preparative Chromatography Techniques

Author : Kurt Hostettmann,Maryse Hostettmann,Andrew Marston
Publisher : Springer Science & Business Media
Page : 146 pages
File Size : 43,5 Mb
Release : 2013-04-17
Category : Science
ISBN : 9783662024928

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Preparative Chromatography Techniques by Kurt Hostettmann,Maryse Hostettmann,Andrew Marston Pdf

Over the last few years several new instrumental techniques have been introduced for chromatographie separations. In addition, rapid developments in existing methods, such as preparative HPLC, have taken place. Despite these advances, how ever, a handbook covering the various preparative aspects ofthe new separation tech niques does not exist. This book is an attempt to fill the gap and to present a compila tion of modern separation techniques that will be useful for researchers faced with day-to-day preparative problems. Numerous examples of separations have been selected in order to show the possibilities (and also the limits) of each technique treated. These are often either applications from our own laboratory or else they reflect the approach we have been following for the isolation of natural products from plant sourees. Owing to the large number of published papers and the diversity of secondary plant constituents. an exhaustive survey of the literature has not been undertaken. We hope, however, that the examples selected will suggest to the reader which technique(s) and which conditions to choose for a particular isolation problem in the field of natural products. For invaluable help in the preparation of the manuscript for this book, we would like to thank Corinne Appolonia and Christine Marston. Lausanne, November 1985 K. Hostettmann M. Hostettmann A . . Marston Foreword Although not many people realize this, isolation and purification of biologically active materials is becoming increasingly crucial.

Author : H. Schmidt-Traub,Michael Schulte,Andreas Seidel-Morgenstern
Publisher : John Wiley & Sons
Page : 545 pages
File Size : 49,7 Mb
Release : 2012-09-27
Category : Science
ISBN : 9783527649303

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Preparative Chromatography by H. Schmidt-Traub,Michael Schulte,Andreas Seidel-Morgenstern Pdf

Completely revised and substantially extended to reflect the developments in this fast-changing field. It retains the interdisciplinary approach that elegantly combines the chemistry and engineering involved to describe the conception and improvement of chromatographic processes. It also covers recent advances in preparative chromatographic processes for the separation of "smaller" molecules using standard laboratory equipment as well as the detailed conception of industrial chemical plants. The increase in biopharmaceutical substances is reflected by new and revised chapters on different modifications of continuous chromatography as well as ion-exchange chromatography and other separation principles widely used in biochromatography. Following an introductory section on the history of chromatography, the current state of research and the design of chromatographic processes, the book goes on to define the general terminology. There then follow sections on stationary phases, selection of chromatographic systems and process concepts. A completely new chapter deals with engineering and operation of chromatographic equipment. Final chapters on modeling and determination of model parameters as well as model based design, optimization and control of preparative chromatographic processes allow for optimal selection of chromatographic processes. Essential for chemists and chemical engineers in the chemical, pharmaceutical, and food industries.

Author : Arne Staby,Anurag S. Rathore,Satinder Ahuja
Publisher : John Wiley & Sons
Page : 608 pages
File Size : 41,8 Mb
Release : 2017-02-02
Category : Technology & Engineering
ISBN : 9781119031178

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Preparative Chromatography for Separation of Proteins by Arne Staby,Anurag S. Rathore,Satinder Ahuja Pdf

Preparative Chromatography for Separation of Proteins addresses a wide range of modeling, techniques, strategies, and case studies of industrial separation of proteins and peptides. • Covers broad aspects of preparative chromatography with a unique combination of academic and industrial perspectives • Presents Combines modeling with compliantce useing of Quality-by-Design (QbD) approaches including modeling • Features a variety of chromatographic case studies not readily accessible to the general public • Represents an essential reference resource for academic, industrial, and pharmaceutical researchers

Author : B.A. Bidlingmeyer
Publisher : Elsevier
Page : 340 pages
File Size : 45,8 Mb
Release : 1987-07-01
Category : Science
ISBN : 0080858414

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Preparative Liquid Chromatography by B.A. Bidlingmeyer Pdf

This volume provides a straightforward approach to isolation and purification problems with a thorough presentation of preparative LC strategy including the interrelationship between the input and output of the instrumentation, while keeping to an application focus. The book stresses the practical aspects of preparative scale separations from TLC isolations through various laboratory scale column separations to very large scale production. It also gives a thorough description of the performance parameters (e.g. throughput, separation quality, etc.) as a function of operational parameters (e.g. particle size, column size, solvent usage, etc.). Experts in the field have contributed a well balanced presentation of separation development strategies from preparative TLC to commercial preparative process with practical examples in a wide variety of application areas such as drugs, proteins, nucleotides, industrial extracts, organic chemicals, enantiomers, polymers, etc.

Author : Toma J. Quintin
Publisher : Unknown
Page : 0 pages
File Size : 48,9 Mb
Release : 2010
Category : Liquid chromatography
ISBN : 1608763161

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Chromatography by Toma J. Quintin Pdf

Chromatography is the collective term for a set of laboratory techniques for the separation of mixtures. It involves passing a mixture dissolved in a "mobile phase" through a stationary phase, which separates the analyte to be measured from other molecules in the mixture and allows it to be isolated. Chromatography may be preparative or analytical. The purpose of preparative chromatography is to separate the components of a mixture for further use (and is thus a form of purification). Analytical chromatography is done normally with smaller amounts of material and is for measuring the relative proportions of analytes in a mixture. This new and important book gathers the latest research from around the globe in the study of chomatography and herein highlights such topics as: analysis of veterinary drugs using chromatographic techniques, liquid chromatography for the determination of mycotoxins in foods, chromatography in the research of phenolic secondary metabolites, and others.

Author : G. Ganetsos,P.E. Barker
Publisher : CRC Press
Page : 812 pages
File Size : 49,7 Mb
Release : 1992-09-16
Category : Science
ISBN : 0824787382

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Preparative and Production Scale Chromatography by G. Ganetsos,P.E. Barker Pdf

Describes the latest developments in the scaling-up and application of chromatographic operations and demonstrates that production-scale chromatography is a powerful and invaluable separation process. The book covers every important process design and reveals actual, immediately applicable techniques and is designed to appeal to design, chemical/biochemical, and research and development engineers, process development managers, bioprocess technologists, analytical and clinical chemists and biochemists, pharmacists, and upper-level undergraduate, graduate, and continuing-education students in these disciplines.

Author : Donald A Wellings
Publisher : Elsevier
Page : 176 pages
File Size : 47,6 Mb
Release : 2011-04-18
Category : Technology & Engineering
ISBN : 0080458858

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A Practical Handbook of Preparative HPLC by Donald A Wellings Pdf

This book is a distillation of twenty years of practical experience of the high pressure liquid chromatography (HPLC) process. Deliberately steering clear of complex theoretical aspects, this book concentrates on the everyday problems associated with the technique, making it perfect for frequent use in the laboratory and for those in the pharmaceutical, agrochemical and biotechnology industries for the analysis and purification of drugs, small molecules, proteins and DNA. This book... •Provides practical, hands-on advice based on years of experience •Will help ensure optimal design, equipment and separation results for your particular task •Presents system layouts from laboratory to process scale •Will help you to devise or improve record-keeping and documentation systems ·Provides practical, hands-on advice based on years of experience ·Will help ensure optimal design, equipment and separation results for your particular task ·Presents system layouts from laboratory to process scale ·Will help you to devise or improve record-keeping and documentation systems

Author : Arne Staby,Anurag S. Rathore,Satinder Ahuja
Publisher : John Wiley & Sons
Page : 577 pages
File Size : 53,7 Mb
Release : 2017-03-06
Category : Technology & Engineering
ISBN : 9781119031109

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Preparative Chromatography for Separation of Proteins by Arne Staby,Anurag S. Rathore,Satinder Ahuja Pdf

Preparative Chromatography for Separation of Proteins addresses a wide range of modeling, techniques, strategies, and case studies of industrial separation of proteins and peptides. • Covers broad aspects of preparative chromatography with a unique combination of academic and industrial perspectives • Presents Combines modeling with compliantce useing of Quality-by-Design (QbD) approaches including modeling • Features a variety of chromatographic case studies not readily accessible to the general public • Represents an essential reference resource for academic, industrial, and pharmaceutical researchers

Author : Teresa Kowalska,Joseph Sherma
Publisher : CRC Press
Page : 441 pages
File Size : 45,7 Mb
Release : 2006-02-03
Category : Medical
ISBN : 9781420005820

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Preparative Layer Chromatography by Teresa Kowalska,Joseph Sherma Pdf

Preparative Layer Chromatography explains how this method is used for separating large quantities of mixtures containing a wide variety of important compounds. It offers a broad review of preparative layer chromatography (PLC) applications and adaptable working procedures for microseparations involving organic, inorganic, and organometallic compoun

Author : Georges Guiochon,Dean G. Shirazi,Attila Felinger
Publisher : Academic Press
Page : 991 pages
File Size : 54,8 Mb
Release : 2006-03-21
Category : Medical
ISBN : 9780123705372

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Fundamentals of Preparative and Nonlinear Chromatography by Georges Guiochon,Dean G. Shirazi,Attila Felinger Pdf

The second edition of Fundamentals of Preparative and Nonlinear Chromatography is devoted to the fundamentals of a new process of purification or extraction of chemicals or proteins widely used in the pharmaceutical industry and in preparative chromatography. This process permits the preparation of extremely pure compounds satisfying the requests of the US Food and Drug Administration. The book describes the fundamentals of thermodynamics, mass transfer kinetics, and flow through porous media that are relevant to chromatography. It presents the models used in chromatography and their solutions, discusses the applications made, describes the different processes used, their numerous applications, and the methods of optimization of the experimental conditions of this process.

Author : Lloyd R. Snyder,Joseph J. Kirkland,John W. Dolan
Publisher : John Wiley & Sons
Page : 831 pages
File Size : 55,9 Mb
Release : 2011-09-20
Category : Science
ISBN : 9781118210390

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Introduction to Modern Liquid Chromatography by Lloyd R. Snyder,Joseph J. Kirkland,John W. Dolan Pdf

The latest edition of the authoritative reference to HPLC High-performance liquid chromatography (HPLC) is today the leading technique for chemical analysis and related applications, with an ability to separate, analyze, and/or purify virtually any sample. Snyder and Kirkland's Introduction to Modern Liquid Chromatography has long represented the premier reference to HPLC. This Third Edition, with John Dolan as added coauthor, addresses important improvements in columns and equipment, as well as major advances in our understanding of HPLC separation, our ability to solve problems that were troublesome in the past, and the application of HPLC for new kinds of samples. This carefully considered Third Edition maintains the strengths of the previous edition while significantly modifying its organization in light of recent research and experience. The text begins by introducing the reader to HPLC, its use in relation to other modern separation techniques, and its history, then leads into such specific topics as: The basis of HPLC separation and the general effects of different experimental conditions Equipment and detection The column—the "heart" of the HPLC system Reversed-phase separation, normal-phase chromatography, gradient elution, two-dimensional separation, and other techniques Computer simulation, qualitative and quantitative analysis, and method validation and quality control The separation of large molecules, including both biological and synthetic polymers Chiral separations, preparative separations, and sample preparation Systematic development of HPLC separations—new to this edition Troubleshooting tricks, techniques, and case studies for both equipment and chromatograms Designed to fulfill the needs of the full range of HPLC users, from novices to experts, Introduction to Modern Liquid Chromatography, Third Edition offers the most up-to-date, comprehensive, and accessible survey of HPLC methods and applications available.

Author : Hassan Y. Aboul-Enein
Publisher : CRC Press
Page : 479 pages
File Size : 42,6 Mb
Release : 2020-10-14
Category : Science
ISBN : 9781000110401

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Analytical and Preparative Separation Methods of Biomacromolecules by Hassan Y. Aboul-Enein Pdf

Reports up-to-date research developments on purifying and isolation large organic molecules. The text provides information on high-performance liquid chromatography and capillary electrophoresis (CE) as tools for analyzing biomacromolecules and developing new biochemical and medicinal compounds. It applies biochemical separation technology to the study of macromolecules such as proteins, polysaccharides, nucleic acids and more.

Author : Dorota Antos
Publisher : LAP Lambert Academic Publishing
Page : 148 pages
File Size : 55,9 Mb
Release : 2009-09
Category : Electronic
ISBN : 3838310764

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Gradient Techniques in Preparative Chromatography by Dorota Antos Pdf

Chromatographic separations are usually realized in isocratic mode, i.e., with constant temperature, pressure or solvent composition of the mobile phase during the process. However, it is well known that the adsorption behavior of the system can be strongly affected by changes of these operating parameters in gradient mode. In the work presented here several aspects of applying the gradient technique for preparative scale of both batch and continuous chromatography are discussed. This analysis is included in the main PART II of the work. It starts from the examination of a batch system consisting of a single column. Subsequently, strategies of using gradient in a continuous separation with a parallel multi-column arrangement and with the SMB system are discussed. Before analyzing the gradient technique the application of mathematical models of process dynamics for predicting chromatographic separations is thoroughly examined in PART I of the work. Moreover, in PART I several aspects of the application of numerical methods for solving the model equations are discussed.

Author : Angelo Talamona
Publisher : BÜCHI Labortechnik AG
Page : 125 pages
File Size : 42,9 Mb
Release : 2005
Category : Electronic
ISBN : 9783033003392

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Laboratory Chromatography Guide by Angelo Talamona Pdf

Author : H. Schmidt-Traub
Publisher : John Wiley & Sons
Page : 485 pages
File Size : 53,5 Mb
Release : 2006-03-06
Category : Technology & Engineering
ISBN : 9783527605903

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Preparative Chromatography by H. Schmidt-Traub Pdf

This interdisciplinary approach combines the chemistry and engineering involved to describe the conception and improvement of chromatographic processes. The book covers recent developments in preparative chromatographic processes for the separation of "smaller" molecules using standard laboratory equipment as well as the detailed conception of industrial chemical plants. Following an introductory section on the history of chromatography, the current state of research and the design of chromatographic processes, the book goes on to define the general terminology. There then follow sections on solid materials and packed columns process concepts. Final chapters on modeling and determination of model parameters, the design and optimization of preparative chromatographic processes and chromatographic reactors allow for the optimum selection of chromatographic systems. Essential for chemists and engineers working in the chemicals and pharmaceutical industries as well as for food technologies, due to the interdisciplinary nature of these processes.