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Principles and Practice of Immunoassay by Christopher P. Price,David J. Newman Pdf
Each chapter of this book aims to explore the basic physical and chemical principles involved in the immunoassay techniques discussed. The book also looks at the optimization and limitations of methodology and concludes with a brief overview of the application of the performance of the technology.
Immunoassay Automation: A Practical Guide describes automation of immunoassay from the practical viewpoint of the clinical laboratory. General introduction and evaluation sections demonstrate principles and practice. A comprehensive selection of available systems are detailed by experts, with a view towards popularity, technical advances, and operational efficiency. This laboratory guide is essential for practitioners in clinical chemistry laboratories, and will have lasting value in the evolution of automated systems. Focuses on automation of immunoassay for the clinical laboratoryEmphasizes principles, method evaluation, and the systems approachAids system selection by evaluation of technical, clinical, operational, and economical parametersContains complete descriptions by experts on the latest automated immunoassay systemsBased upon the editor's well-received workshops on automated immunoassay
The fourth edition of The Immunoassay Handbook provides an excellent, thoroughly updated guide to the science, technology and applications of ELISA and other immunoassays, including a wealth of practical advice. It encompasses a wide range of methods and gives an insight into the latest developments and applications in clinical and veterinary practice and in pharmaceutical and life science research. Highly illustrated and clearly written, this award-winning reference work provides an excellent guide to this fast-growing field. Revised and extensively updated, with over 30% new material and 77 chapters, it reveals the underlying common principles and simplifies an abundance of innovation. The Immunoassay Handbook reviews a wide range of topics, now including lateral flow, microsphere multiplex assays, immunohistochemistry, practical ELISA development, assay interferences, pharmaceutical applications, qualitative immunoassays, antibody detection and lab-on-a-chip. This handbook is a must-read for all who use immunoassay as a tool, including clinicians, clinical and veterinary chemists, biochemists, food technologists, environmental scientists, and students and researchers in medicine, immunology and proteomics. It is an essential reference for the immunoassay industry. Provides an excellent revised guide to this commercially highly successful technology in diagnostics and research, from consumer home pregnancy kits to AIDS testing.www.immunoassayhandbook.com is a great resource that we put a lot of effort into. The content is designed to encourage purchases of single chapters or the entire book. David Wild is a healthcare industry veteran, with experience in biotechnology, pharmaceuticals, medical devices and immunodiagnostics, which remains his passion. He worked for Amersham, Eastman-Kodak, Johnson & Johnson, and Bristol-Myers Squibb, and consulted for diagnostics and biotechnology companies. He led research and development programs, design and construction of chemical and biotechnology plants, and integration of acquired companies. Director-level positions included Research and Development, Design Engineering, Operations and Strategy, for billion dollar businesses. He retired from full-time work in 2012 to focus on his role as Editor of The Immunoassay Handbook, and advises on product development, manufacturing and marketing. Provides a unique mix of theory, practical advice and applications, with numerous examples Offers explanations of technologies under development and practical insider tips that are sometimes omitted from scientific papers Includes a comprehensive troubleshooting guide, useful for solving problems and improving assay performancee Provides valuable chapter updates, now available on www.immunoassayhandbook.com
Immunoassay development is a multidisciplinary activity involving a wide range of skills possessed by few laboratories. This presentation of tried and tested methods should enable scientists and researchers in the pharmaceutical and related industries to more rapidly and effectively develop immunoassays upon which their work is becoming heavily dependent.; Important methods are included for preparing Lapten-protein conjugates and raising the necessary antibodies, concentrating on polyclonal sera, as well as methods for the synthesis of radio and enzyme labelled tracers. Particular attention is paid to the requirements of the regulatory authorities such as the FDA (Food and Drug Administration) with respect to assay validation. Further chapters deal with assay development and optimization, curve fitting and quality control procedures.
Containing updated and new information on advanced technology - including micro and nanoscale immunoassays - this text provides a mix of practical information coupled with a review of clincal applications and practical examples.
Immunoassay by Eleftherios P. Diamandis,Theodore K. Christopoulos Pdf
Immunoassays are among the most powerful and sensitive technologies now available for patient diagnosis and monitoring. This book is an indispensable guide to information on the theory and practice of immunoassays. It discusses the scientific basis of these technologies in a logical, organized, and heuristic manner and provides protocols for specific assays. The contents of this unique book are balanced among theory, practical issues, quality control, automation, and subspecialty areas, making it ideal for health science students, laboratory scientists, and clinicians. Presents up-to-date information Provides extensive cross-referencing Covers theory and practice in full detail Written by leading authorities
Author : James P. Gosling Publisher : Oxford University Press, USA Page : 304 pages File Size : 42,6 Mb Release : 2000 Category : Medical ISBN : 0199637113
Immunoassays is the first practical volume designed to help any biologist develop an immunoassay of any common format for any suitable analyte. The basic principles are described and the choices of assay types and formats listed. Methods for raising and for characterizing antibodies, for preparing radioactive, enzymatic, fluorescent and other labels, are described. Solid-phase reagents, standards and immunogens are explained, with procedures for their preparation. But most components can be obtained commercially and comprehensive lists of suppliers are given. Approaches to combining the reagents and optimizing the required assay are described, including how to eliminate common interferences, and thorough validation procedures are given. Manual and computer methods for the calculation of concentrations are explained. Since only assays that are within control give valid results, a graded and comprehensive approach to internal and external quality assurance methods is detailed, including how to prepare control samples.
An Introduction to Radioimmunoassay and Related Techniques by T. Chard Pdf
Immunoassay procedures (isotopic and non-isotopic) have become one of the single most important techniques in present-day diagnostic medicine. This book is designed as an introductory test for the staff of clinical research laboratories who conduct or intend to conduct such techniques, and will be of great value to the clinicians who make use of such services. The volume takes a three-pronged approach in it's in-depth presentation: explanation of the basic principles and applications of radioimmunoassays and non-isotopic immunoassays; practical illustrations of the various steps involved in immunoassays; discussion of the problems and pitfalls in immunoassays and how to avoid them. This fifth revised edition is a worthy successor to it's predecessors in this famous "Laboratory Techniques" series.
Immunodiagnositics: Principles and Practice by S.C. Rastogi Pdf
S.C. Rastogi, Formerly Professor Of Biological Sciences, B.I.T.S., Pilani, Has Vast Experience Of Teaching And Research Spanning More Than 40 Years. After His Training In Molecular Biology At Tata Institute Of Fundamental Research (Tifr), Mumbai, He Worked Constantly To Modernise Biology Courses At Bits. He Has Taught And Supervised Doctoral Research In Computer Applications In Molecular Biology, Enzyme Biotechnology And Physiology, And Has Successfully Completed Several Research Projects. He Has Edited Many Proceedings Of Scientific Symposia And Authored Research Papers And Books In The Field Of Bioinformatics, Biochemistry, Physiology, Molecular Biology And Biotechnology. As A Biologist Of Repute, And Accredited With Many Educational Innovations, He Has Been A Constant Participant In Course Development Work At Bits And Otheruniversities.
Immunochemistry of Solid-Phase Immunoassay by John E. Butler Pdf
Immunochemistry of Solid-Phase Immunoassay fills a niché in the field of immunoassay and immunology. Although solid-phase immunoassay constitutes a major technology in biology and medicine, there is no comprehensive source devoted to the immunochemical principles involved. As a result, this book will benefit students, technicians, and researchers who use this technology, as well as immunodiagnostic and biotech companies who develop the technology. The book is not a methods manual; instead, it incorporates the concepts, data, and opinions of more than 25 investigators working in this field. Topics discussed include: the chemistry of solid-phases, the behavior or antibodies and antigens on solid phases, membrane solid-phases, reaction kinetics, antigen quantitation, enzyme systems, photophysics, immunochemical considerations in data analyses, multianalyte assays and occupancy concepts, antibody quantitation, streptavidin, a review of data analysis software, and solid-phase peptide immunoassay.
Principles and Practice of Bioanalysis by Richard F. Venn Pdf
Principles and Practice of Bioanalysis provides a guide to the methods available and the techniques currently used in this field. It provides up to the minute information and guidance on the methods and strategy used in developing and running ultra-trace analyses for drugs, metabolites and other substances. The authors writes in an informal and didactic style, offering a logical path through the problems of small molecule (bio)analysis and enables readers to choose appropriate methods of analysis for their needs. Principles and Practice of Bioanalysis provides an overview of analytical methods for analytical scientists within the pharmaceutical industry, research and development, the agrochemical industry, and scientists in the health service, biology and biochemistry. It also gives postgraduate students a useful reference for their research methods.
Author : Paul P. Glasziou,Les Irwig,Jeffrey K. Aronson Publisher : John Wiley & Sons Page : 376 pages File Size : 44,7 Mb Release : 2008-04-30 Category : Medical ISBN : 9780470695616
Evidence-Based Medical Monitoring by Paul P. Glasziou,Les Irwig,Jeffrey K. Aronson Pdf
Monitoring is a major component of management of chronic diseases such as diabetes, cardiovascular disease, arthritis and depression. Yet poor monitoring means healthcare costs are rising. This book discusses how monitoring principles adopted in other spheres such as clinical pharmacology and evidence-based medicine can be applied to chronic disease in the global setting. With contributions from leading experts in evidence-based medicine, it is a ground-breaking text for all involved in delivery of better and more effective management of chronic illnesses.
Lateral Flow Immunoassay by Raphael Wong,Harley Tse Pdf
Due to the simplicity, relative accuracy, fast result reporting, and user-friendliness of lateral flow immunoassay, its use has undergone tremendous growth in the diagnostic industry in the last few years. Such technology has been utilized widely and includes pregnancy and woman's health determination, cardiac and emergency conditions monitoring and testing, infectious disease including Flu screening, cancer marker screening, and drugs abuse testing. This book covers the scope of utilization, the principle of the technology, the patent concerns, information on the development and production of the test device and specific applications will be of interest to the diagnostic industry and the general scientific community.
Immunosensing for Detection of Protein Biomarkers by Huangxian Ju,Guosong Lai,Feng Yan Pdf
Immunosensing for Detection of Protein Biomarkers not only introduces the principles, methods, and classification of immunoassay, but also presents the latest achievements in areas such as electrochemical immunosensors, nanoprobe-based immunoassay, chemiluminescence immunoassay, electrochemiluminescent immunoassay, multianalyte immunoassay, optical imaging for immunoassay, signal amplification for immunoassay, and so on. In recent years, immunosensing and immunoassay methods have attracted considerable interest due to their applications in different fields, particularly clinical diagnosis. Although a large number of academic papers in immunosensing and immunoassay have been published in different journals recently, it is still a difficult and time-consuming task for researchers, especially those new to the area, to understand the principles, methods, and research progress of immunosensing. Based on the research experience of the authors and their research groups, this book offers readers with new research ideas to develop immunosensing methodology. As a monograph, it offers deeper and more complete coverage than review papers, which only report certain aspects of progress. Grounded in the research experience of Professor Ju’s research group, the book focuses on immunosensing for detection of protein biomarkers, summarizing understanding, research, and practice on immunosensing methodology in detection of protein biomarkers. Presents the latest research and thinking on immunosensing for detection of protein biomarkers Offers current techniques, and looks to the development of new methodologies Offers the latest developments in various aspects of immunosensing, including electrochemiluminescent immunoassay, multianalyte immunoassay, optical imaging immunoassay, and signal amplification immunoassay Offers readers new ideas to research and develop immunosensing methodologies for the future
