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Author : W. Borchard
Publisher : Unknown
Page : 136 pages
File Size : 40,8 Mb
Release : 1991
Category : Technology & Engineering
ISBN : UCSD:31822015323322
Author : Susumu Uchiyama,Fumio Arisaka,Walter F. Stafford,Tom Laue
Publisher : Springer
Page : 532 pages
File Size : 52,9 Mb
Release : 2016-05-13
Category : Science
ISBN : 9784431559856
This book introduces analytical ultracentrifugation (AUC) as a whole, covering essential theoretical and practical aspects as well as its applications in both biological and non-biological systems. Comprehensive characterizations of macromolecules in a solution are now routinely required not only for understanding the solution system but also for producing a solution with better properties. Analytical ultracentrifugation is one of most powerful and reliable techniques for studying the biophysical behavior of solutes in solution. In the last few years, there have been steady advances made in hardware, software, and applications for AUC. This book provides chapters that cover everything essential for beginners to the most advanced users and also offer updated knowledge of the field on advances in hardware, software, and applications. Recent development of hardware described in this book covers new detection systems that give added dimensions to AUC. Examples of data analysis with essential theoretical explanations for advanced and recently updated software are also introduced. Besides AUC of biological systems including membrane proteins and biopharmaceuticals, AUC applications for non-biological questions are included. AUC studies under non-ideal conditions such as highly concentrated solutions and solutions with high salt concentration are also included. The contributors to this book are leading researchers in the fields of solution biophysics and physical chemistry who extensively employ AUC analysis for their research. From this published work, one can gain new and comprehensive knowledge of recent AUC analysis.
Author : H. Cölfen
Publisher : Springer
Page : 223 pages
File Size : 53,7 Mb
Release : 2003-07-01
Category : Technology & Engineering
ISBN : 9783540487036
The basis for this volume is the 11th Symposium on Analytical Ultracentrifugation held in March 25-26, 1999 at the University of Potsdam, Germany. This book presents a comprehensive collection of 33 contributions from leading scientists in this field including: Technical and methodological innovations.- Innovations in data analysis.- Hydrodynamics/Modelling.- Synthetic polymers, colloids and supramolecular systems.- Biological systems.- Interacting systems and assemblies. In contrast to the increasing significance of analytical ultracentrifugation, related modern books are very rare. Therefore, this volume will be a helpful source of information to anyone who wants to catch up with the most recent developments and results related to this important analytical method.
Author : W. Borchard,A. Straatmann
Publisher : Springer Science & Business Media
Page : 168 pages
File Size : 45,9 Mb
Release : 2002-02-11
Category : Science
ISBN : 9783540424895
This volume includes 20 contributions of the 12th meeting on Analytical Ultracentrifugation from March 1-2, 2001 in Duisburg, Germany. Various fields of ultracentrifugation are covered concerning research problems in biochemistry, biophysical chemistry and macromolecular chemistry as well as interacting systems. New investigations concerning the sedimentation theory are presented. The phase transition of gels is dealt with, as is the sedimentation-diffusion equilibrium of gels. One section contains the hydrodynamics of biopolymers.
Author : M. D. Lechner,Lars Börger
Publisher : Springer Science & Business Media
Page : 180 pages
File Size : 55,8 Mb
Release : 2004-11-24
Category : Science
ISBN : 354020203X
This volume includes 19 contributions to the 13th International Symposium on Analytical Ultracentrifugation which took place at the university of Osnabrück on March 6th and 7th, 2003. The contributions from leading scientists cover a broad spectrum of topics concerning: Technical Methods, Data Analysis, Innovations; Polymers, Colloids, Supramolecular Systems; Biological and Interaction Systems; Hydrodynamics and Modelling. Due to the increasing significance of Analytical Ultracentrifugation for both scientific and technical applications, this book will be an essential source of information with respect to recent developments and results related to this important analytical method.
Author : Stephen E Harding,David Scott,Arther Rowe
Publisher : Royal Society of Chemistry
Page : 612 pages
File Size : 50,5 Mb
Release : 2007-12-16
Category : Science
ISBN : 9781847552617
Analytical ultracentrifugation has become an increasingly important technique for monitoring the size and shape of biological macromolecules. Analytical Ultracentrifugation: Techniques and Methods contains contributions from experts in the field, bringing together the multitude of developments that have taken place in instrumentation and analysis over the past decade into a single volume. This book covers the latest methods in analysis along with an extensive introduction for the novice user. Analysis methods in both sedimentation velocity and sedimentation equilibrium are discussed at length. Protein, protein/DNA, membrane proteins and polymer systems are also explored, along with software developments and non-ideality.
Author : J. Behlke
Publisher : Springer
Page : 228 pages
File Size : 46,9 Mb
Release : 1995-11-20
Category : Science
ISBN : UCSD:31822009014655
The 9th Symposium on Analytical Ultracentrifugation (AUC) which was held at the Max DelbrA1/4ck Center for Molecular Medicine in Berlin-Buch, March, 1995, is the basis of this volume. It presents an actual survey of recent developments and results of ultracentrifugation methods in the frame of the following topics: Reversible Association Reactions.- Analysis of Supramolecular Structures Including Modeling.- Size Distribution Analysis and Gel Formation.- Hardware and Software Developments.
Author : Todd M. Schuster,Thomas M. Laue
Publisher : Springer Science & Business Media
Page : 359 pages
File Size : 54,7 Mb
Release : 2012-12-06
Category : Science
ISBN : 9781468468281
There are numerous examples in the history of science when the parallel develop ments of two or more disciplines, methodologies, technologies or theoretical in sights have converged to produce significant scientific advances. The decades following the 1950s have produced several such significant advances, as a result of a convergence of developments in molecular biology and in solid state-based electronics instrumentation. Since one of these areas of significant advancement, analytical ultracentrifu gation, has been undergoing a renaissance, we thought it would be a useful activity to call upon a group of researchers who have been developing either the experi mental or theoretical aspects of the methodology and gather in one place a group of articles summarizing the current status of the field. The success of recombinant DNA methodologies at producing biologically active macromolecules of commer cial interest has evoked interests in mechanisms of function. Pursuit of the related questions has emphasized the importance of studies of macromolecular binding and interaction. Several contributions to this volume remind us that analytical ultra centrifugation is rigorously based on solid thermodynamic theory and, as such, is fully capable of providing comprehensive quantitative descriptions of molecular interactions in solution. Furthermore, a number of the chapters provide examples, along with innovative methods for carrying out these characterizations. The past decade has seen several developments that reflect the rebirth of interest in analytical ultracentrifugation.
Author : Symposium on Analytical Ultracentrifugation
Publisher : Unknown
Page : 128 pages
File Size : 45,9 Mb
Release : 1995
Category : Electronic
ISBN : OCLC:901516237
Author : Rainer Jaenicke,Helmut Durchschlag
Publisher : Springer
Page : 212 pages
File Size : 44,5 Mb
Release : 1997-11
Category : Science
ISBN : UCSD:31822025565326
This volume contains the contributions of the 10th Symposium on Analytical Ultracentrifugation, held in Regensburg, Germany, on March 13th to 14th, 1997. Topics in the following fields were presented: Technical innovations; innovations in data analysis; modeling; biological systems; ions and polyelectrolytes; polymers, colloids, supramolecular systems; emulsions, gels, and dispersions; interacting systems and assemblies.
Author : Christine Wandrey,Helmut Cölfen
Publisher : Springer
Page : 0 pages
File Size : 43,5 Mb
Release : 2010-02-12
Category : Science
ISBN : 3642067409
The 14th International Symposium on Analytical Ultracentrifugation was held in March 2005 at the École Polytechnique Fédérale de Lausanne in Switzerland. This book presents a comprehensive collection of 21 contributions from leading scientists in this field covering a broad spectrum of topics and presenting recent progress concerning instrumentation, data analysis and modeling, biological systems, particles, colloids, synthetic macromolecules, interacting systems. Analytical Ultracentrifugation is becoming increasingly important in both academic and industrial applications. Due to the versatility of this fascinating and powerful technique, information and original publications are widespread and comprehensive collections are rare. Therefore, this volume presents a valuable source for biologists, chemists, materials scientists, and physicists interested in most recent information, results and development related to this important analytical method.
Author : M.D. Lechner
Publisher : Steinkopff
Page : 120 pages
File Size : 49,7 Mb
Release : 2014-03-12
Category : Technology & Engineering
ISBN : 3662156717
Ultracentrifugation contains the proceedings of the VIII. same-named symposium held in 1993, at Osnabrück, FRG. The volume presents the current concepts in analytical ultracentrifugation (AUC) including: . biochemical and biophysical applications . characterization of polyelectrolytes, synthetic polymers, gels and lattices . modern, fully computerized analytical ultracentrifuge Optima XL-A . future technical developments.
Author : Walter Maechtle,Lars Börger
Publisher : Springer Science & Business Media
Page : 249 pages
File Size : 44,7 Mb
Release : 2006-07-09
Category : Technology & Engineering
ISBN : 9783540262183
This book is divided into chapters covering instrumentation, sedimentation velocity runs, density gradient runs, application examples and future developments. In particular, the detailed application chapter demonstrates the versatility and power of AUC by means of many interesting and important industrial examples. Thus the book concentrates on practical aspects rather than details of centrifugation theory.
Author : Stephen E. Harding,Arthur J. Rowe,John C. Horton
Publisher : Unknown
Page : 656 pages
File Size : 41,6 Mb
Release : 1992
Category : Science
ISBN : STANFORD:36105002260789
This is the first book of its kind to appear for nearly two decades and gives as comprehensive a coverage as is possible of the present state-of-the-art.
Author : Peter Schuck,Huaying Zhao,Chad A. Brautigam,Rodolfo Ghirlando
Publisher : CRC Press
Page : 302 pages
File Size : 40,6 Mb
Release : 2016-01-05
Category : Medical
ISBN : 9781498751162
Analytical ultracentrifugation (AUC) can supply rich information on the mass, shape, size distribution, solvation, and composition of macromolecules and nanoscopic particles. It also provides a detailed view of their reversible single- or multi-component interactions over a wide range of affinities. Yet this powerful technique has been hard to master in mainstream molecular sciences due to a lack of comprehensive books on the subject. Filling this gap in the literature of biophysical methodology, Basic Principles of Analytical Ultracentrifugation explains the fundamentals in the theory and practice of AUC. The book provides you with up-to-date experimental information to confidently practice AUC. You will understand the basic concepts, full potential, and possible pitfalls of AUC as well as appreciate the current relevance of past work in the field. The book first introduces the basic principles and technical setup of an AUC experiment and briefly describes the optical systems used for detection. It then explores the ultracentrifugation experiment from a macromolecular standpoint, offering a detailed physical picture of the sedimentation process and relevant macromolecular parameters. The authors present important practical aspects for conducting an experiment, including sample preparation, data acquisition and data structure, and the execution of the centrifugal experiment. They also cover instrument calibration and quality control experiments.
