Protein Crystallization

Author : Alexander Wlodawer,Zbigniew Dauter,Mariusz Jaskolski
Publisher : Humana Press
Page : 672 pages
File Size : 49,9 Mb
Release : 2017-06-02
Category : Science
ISBN : 1493969986

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Protein Crystallography by Alexander Wlodawer,Zbigniew Dauter,Mariusz Jaskolski Pdf

This volume provides methods for modern macromolecular crystallography, including all steps leading to crystal structure determination and analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Protein Crystallography aims to ensure successful results in the further study of this vital field.

Author : Terese M. Bergfors
Publisher : Internat'l University Line
Page : 505 pages
File Size : 51,5 Mb
Release : 2009
Category : Medical
ISBN : 9780972077446

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Protein Crystallization by Terese M. Bergfors Pdf

Author : Jan Drenth
Publisher : Springer Science & Business Media
Page : 368 pages
File Size : 55,9 Mb
Release : 2002-02-15
Category : Science
ISBN : 0387985875

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Principles of Protein X-ray Crystallography by Jan Drenth Pdf

New textbooks at all levels of chemistry appear with great regularity. Some fields such as basic biochemistry, organic reaction mechanisms, and chemical thermodynamics are well represented by many excellent texts, and new or revised editions are published sufficiently often to keep up with progress in research. However, some areas of chemistry, especially many of those taught at the graduate level, suffer from a real lack of up to-date textbooks. The most serious needs occur in fields that are rapidly changing. Textbooks in these subjects usually have to be written by scientists actually involved in the research that is advancing the field. It is not often easy to persuade such individuals to set time aside to help spread the knowledge they have accumulated. Our goal, in this series, is to pinpoint areas of chemistry where recent progress has outpaced what is covered in any available textbooks, and then seek out and persuade experts in these fields to produce relatively concise but instructive intro ductions to their fields. These should serve the needs of one-semester or one-quarter graduate courses in chemistry and biochemistry. In some cases, the availability of texts in active research areas should help stimulate the creation of new courses. Charles R. Cantor v Preface to the Second Edition Since the publication of the previous edition in 1994, X-ray crystallography of proteins has advanced by improvements in existing techniques and by addition of new techniques.

Author : Allan Myerson
Publisher : Butterworth-Heinemann
Page : 304 pages
File Size : 54,5 Mb
Release : 2002-01-08
Category : Science
ISBN : 0080533515

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Handbook of Industrial Crystallization by Allan Myerson Pdf

Crystallization is an important separation and purification process used in industries ranging from bulk commodity chemicals to specialty chemicals and pharmaceuticals. In recent years, a number of environmental applications have also come to rely on crystallization in waste treatment and recycling processes. The authors provide an introduction to the field of newcomers and a reference to those involved in the various aspects of industrial crystallization. It is a complete volume covering all aspects of industrial crystallization, including material related to both fundamentals and applications. This new edition presents detailed material on crystallization of biomolecules, precipitation, impurity-crystal interactions, solubility, and design. Provides an ideal introduction for industrial crystallization newcomers Serves as a worthwhile reference to anyone involved in the field Covers all aspects of industrial crystallization in a single, complete volume

Author : Naomi E. Chayen
Publisher : Internat'l University Line
Page : 300 pages
File Size : 55,9 Mb
Release : 2007
Category : Science
ISBN : 097207743X

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Protein Crystallization Strategies for Structural Genomics by Naomi E. Chayen Pdf

Author : Anonim
Publisher : Academic Press
Page : 336 pages
File Size : 55,5 Mb
Release : 2009-05-29
Category : Science
ISBN : 0080961592

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Membrane Protein Crystallization by Anonim Pdf

This volume of Current Topics in Membranes focuses on Membrane Protein Crystallization, beginning with a review of past successes and general trends, then further discussing challenges of mebranes protein crystallization, cell free production of membrane proteins and novel lipids for membrane protein crystallization. This publication also includes tools to enchance membrane protein crystallization, technique advancements, and crystallization strategies used for photosystem I and its complexes, establishing Membrane Protein Crystallization as a needed, practical reference for researchers.

Author : Eaton E. Lattman,Patrick J. Loll
Publisher : JHU Press
Page : 149 pages
File Size : 49,6 Mb
Release : 2008-04-28
Category : Science
ISBN : 9780801896842

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Protein Crystallography by Eaton E. Lattman,Patrick J. Loll Pdf

The proteome remains a mysterious realm. Researchers have determined the structures of only a small fraction of the proteins encoded by the human genome. Crystallography continues to be the primary method used to determine the structures of the remaining unknown proteins. This imaging technique uses the diffraction of X-rays to determine a protein’s three-dimensional molecular structure. Drawing on years of research and teaching experience, Eaton E. Lattman and Patrick J. Loll use clear examples and abundant illustrations to provide a concise and accessible primer on protein crystallography. Discussing the basics of diffraction, the behavior of two- and three-dimensional crystals, phase determination (including MIR and MAD phasing and molecular replacement), the Patterson function, and refinement, Lattman and Loll provide a complete overview of this important technique, illuminated by physical insights. The crisp writing style and simple illustrations will provide beginner crystallographers with a guide to the process of unraveling protein structure.

Author : So Iwata
Publisher : Internat'l University Line
Page : 392 pages
File Size : 46,7 Mb
Release : 2003
Category : Science
ISBN : 0963681796

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Methods and Results in Crystallization of Membrane Proteins by So Iwata Pdf

Author : Marc L. Pusey,Ramazan Savaş Aygün
Publisher : Springer
Page : 245 pages
File Size : 50,9 Mb
Release : 2017-11-27
Category : Computers
ISBN : 9783319589374

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Data Analytics for Protein Crystallization by Marc L. Pusey,Ramazan Savaş Aygün Pdf

This unique text/reference presents an overview of the computational aspects of protein crystallization, describing how to build robotic high-throughput and crystallization analysis systems. The coverage encompasses the complete data analysis cycle, including the set-up of screens by analyzing prior crystallization trials, the classification of crystallization trial images by effective feature extraction, the analysis of crystal growth in time series images, the segmentation of crystal regions in images, the application of focal stacking methods for crystallization images, and the visualization of trials. Topics and features: describes the fundamentals of protein crystallization, and the scoring and categorization of crystallization image trials; introduces a selection of computational methods for protein crystallization screening, and the hardware and software architecture for a basic high-throughput system; presents an overview of the image features used in protein crystallization classification, and a spatio-temporal analysis of protein crystal growth; examines focal stacking techniques to avoid blurred crystallization images, and different thresholding methods for binarization or segmentation; discusses visualization methods and software for protein crystallization analysis, and reviews alternative methods to X-ray diffraction for obtaining structural information; provides an overview of the current challenges and potential future trends in protein crystallization. This interdisciplinary work serves as an essential reference on the computational and data analytics components of protein crystallization for the structural biology community, in addition to computer scientists wishing to enter the field of protein crystallization.

Author : Abel Moreno
Publisher : MDPI
Page : 91 pages
File Size : 48,8 Mb
Release : 2019-01-15
Category : Electrical engineering. Electronics. Nuclear engineering
ISBN : 9783038975199

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Protein Crystallization under the Presence of an Electric Field Special Issue Editor Abel Moreno MDPI • by Abel Moreno Pdf

This book is a printed edition of the Special Issue "Protein Crystallization under the Presence of an Electric Field" that was published in Crystals

Author : Jan Drenth
Publisher : Springer Science & Business Media
Page : 355 pages
File Size : 53,8 Mb
Release : 2013-03-09
Category : Science
ISBN : 9781475730920

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Principles of Protein X-ray Crystallography by Jan Drenth Pdf

New textbooks at all levels of chemistry appear with great regularity. Some fields such as basic biochemistry, organic reaction mechanisms, and chemical thermodynamics are well represented by many excellent texts, and new or revised editions are published sufficiently often to keep up with progress in research. However, some areas of chemistry, especially many of those taught at the graduate level, suffer from a real lack of up to-date textbooks. The most serious needs occur in fields that are rapidly changing. Textbooks in these subjects usually have to be written by scientists actually involved in the research that is advancing the field. It is not often easy to persuade such individuals to set time aside to help spread the knowledge they have accumulated. Our goal, in this series, is to pinpoint areas of chemistry where recent progress has outpaced what is covered in any available textbooks, and then seek out and persuade experts in these fields to produce relatively concise but instructive intro ductions to their fields. These should serve the needs of one-semester or one-quarter graduate courses in chemistry and biochemistry. In some cases, the availability of texts in active research areas should help stimulate the creation of new courses. Charles R. Cantor v Preface to the Second Edition Since the publication of the previous edition in 1994, X-ray crystallography of proteins has advanced by improvements in existing techniques and by addition of new techniques.

Author : Carola Hunte,Gebhard von Jagow,Hermann Schagger
Publisher : Elsevier
Page : 316 pages
File Size : 55,6 Mb
Release : 2003-01-05
Category : Science
ISBN : 9780080536170

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Membrane Protein Purification and Crystallization by Carola Hunte,Gebhard von Jagow,Hermann Schagger Pdf

This second edition of Membrane Protein Purification and Crystallization, A Practical Guide is written for bench scientists working in the fields of biochemistry, biology, and proteomic research. This guide presents isolation and crystallization techniques in a concise form, emphasizing the critical aspects unique to membrane proteins. It explains the principles of the methods and provides protocols of general use, permitting researchers and students new to this area to adapt these techniques to their particular needs. This edition is not only an update but is comprised mainly of new contributions. It is the first monograph compiling the essential approaches for membrane protein crystallization, and emphasizes recent progress in production and purification of recombinant membrane proteins. Provides general guidelines and strategies for isolation and crystallization of membrane proteins Gives detailed protocols that have wide application, and low specialized equipment needs Emphasizes recent progress in production and purification of recombinant membrane proteins, especially of histidine-tagged and other affinity-epitope-tagged proteins Summarizes recent developments of Blue-Native PAGE, a high resolution separation technique, which is independent of the use of recombinant techniques, and is especially suited for proteomic analyses of membrane protein complexes Gives detailed protocols for membrane protein crystallization, and describes the production and use of antibody fragments for high resolution crystallization Presents a comprehensive guide to 2D-crystallization of membrane proteins

Author : Rajni Hatti-Kaul,Bo Mattiasson
Publisher : CRC Press
Page : 688 pages
File Size : 51,6 Mb
Release : 2003-02-05
Category : Medical
ISBN : 0203911687

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Isolation and Purification of Proteins by Rajni Hatti-Kaul,Bo Mattiasson Pdf

This publication details the isolation of proteins from biological materials, techniques for solid-liquid separation, concentration, crystallization, chromatography, scale-up, process monitoring, product formulation, and regulatory and commercial considerations in protein production. The authors discuss the release of protein from a biological host, selectivity in affinity chromatography, precipitation of proteins (both non-specific and specific), extraction for rapid protein isolation, adsorption as an initial step for the capture of proteins, scale-up and commercial production of recombinant proteins, and process monitoring in downstream processing.

Author : Jaime Gómez Morales
Publisher : MDPI
Page : 184 pages
File Size : 45,9 Mb
Release : 2019-09-23
Category : Science
ISBN : 9783039214037

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Biological Crystallization by Jaime Gómez Morales Pdf

For at least six hundred million years, life has been a fascinating laboratory of crystallization, referred to as biomineralization. During this huge lapse of time, many organisms from diverse phyla have developed the capability to precipitate various types of minerals, exploring distinctive pathways for building sophisticated structural architectures for different purposes. The Darwinian exploration was performed by trial and error, but the success in terms of complexity and efficiency is evident. Understanding the strategies that those organisms employ for regulating the nucleation, growth, and assembly of nanocrystals to build these sophisticated devices is an intellectual challenge and a source of inspiration in fields as diverse as materials science, nanotechnology, and biomedicine. However, “Biological Crystallization” is a broader topic that includes biomineralization, but also the laboratory crystallization of biological compounds such as macromolecules, carbohydrates, or lipids, and the synthesis and fabrication of biomimetic materials by different routes. This Special Issue collects 15 contributions ranging from biological and biomimetic crystallization of calcium carbonate, calcium phosphate, and silica-carbonate self-assembled materials to the crystallization of biological macromolecules. Special attention has been paid to the fundamental phenomena of crystallization (nucleation and growth), and the applications of the crystals in biomedicine, environment, and materials science.

Author : Wolfgang Aehle
Publisher : John Wiley & Sons
Page : 520 pages
File Size : 44,9 Mb
Release : 2007-11-05
Category : Science
ISBN : 3527316892

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Enzymes in Industry by Wolfgang Aehle Pdf

Weltweit führende Experten geben einen aktuellen Überblick über den gesamten Bereich der Herstellung und Verwendung von Enzymen in der Industrie: - bei der Herstellung von Massengütern wie Glukose oder Fruktose - in der Textil-, Papier-, Nahrungsmittel und Tierfutterindustrie - in der klinischen Diagnose und Therapie - in der Gentechnologie In mehreren ausführlichen Tabellen sind zahlreiche Daten von Enzymen zusammengestellt. Ein umfassendes Literaturverzeichnis ermöglicht einen schellen Zugriff auf Primärliteratur. Gesetzliche Bestimmungen für den sicheren und legalen Gebrauch von Enzymen runden das Buch an. Aus Rezensionen voriger Auflagen: 'Die Qualität...ist so hervorragend, dass es uneingeschränkt als ideale Lektüre jedem Studenten, der eine Einführung in das Gebiet sucht, empfohlen werden kann. ...'Enzymes in Industry' sollte seinen Platz in jeder Bibliothek haben, sei es in der Industrie oder Hochschule, wo es mit Sicherheit oft seinen Nutzen finden wird.' (The Genetic Engineer and Biotechnologist) 'Das Buch ist auf dem neuesten Stand und trotz der Beteiligung von fast 50 Autoren leicht zu lesen. ... Naturwissenschaftler, die sich mit Enzymen beschäftigen, sollten dieses Buch in ihrer Bibliothek haben. Das Buch wird aber auch für den Marketing-Spezialisten, der sich einen Überblick über gegenwärtige und künftige Anwendungen von Enzymen innerhalb und außerhalb der Lebensmittelindustrie machen will, von großem Nutzen sein.' (Angewandte Chemie)