Protein Crystallography

Author : Jan Drenth
Publisher : Springer Science & Business Media
Page : 355 pages
File Size : 42,8 Mb
Release : 2013-03-09
Category : Science
ISBN : 9781475730920

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Principles of Protein X-ray Crystallography by Jan Drenth Pdf

New textbooks at all levels of chemistry appear with great regularity. Some fields such as basic biochemistry, organic reaction mechanisms, and chemical thermodynamics are well represented by many excellent texts, and new or revised editions are published sufficiently often to keep up with progress in research. However, some areas of chemistry, especially many of those taught at the graduate level, suffer from a real lack of up to-date textbooks. The most serious needs occur in fields that are rapidly changing. Textbooks in these subjects usually have to be written by scientists actually involved in the research that is advancing the field. It is not often easy to persuade such individuals to set time aside to help spread the knowledge they have accumulated. Our goal, in this series, is to pinpoint areas of chemistry where recent progress has outpaced what is covered in any available textbooks, and then seek out and persuade experts in these fields to produce relatively concise but instructive intro ductions to their fields. These should serve the needs of one-semester or one-quarter graduate courses in chemistry and biochemistry. In some cases, the availability of texts in active research areas should help stimulate the creation of new courses. Charles R. Cantor v Preface to the Second Edition Since the publication of the previous edition in 1994, X-ray crystallography of proteins has advanced by improvements in existing techniques and by addition of new techniques.

Author : Eaton E. Lattman,Patrick J. Loll
Publisher : JHU Press
Page : 149 pages
File Size : 54,7 Mb
Release : 2008-04-28
Category : Science
ISBN : 9780801896842

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Protein Crystallography by Eaton E. Lattman,Patrick J. Loll Pdf

The proteome remains a mysterious realm. Researchers have determined the structures of only a small fraction of the proteins encoded by the human genome. Crystallography continues to be the primary method used to determine the structures of the remaining unknown proteins. This imaging technique uses the diffraction of X-rays to determine a protein’s three-dimensional molecular structure. Drawing on years of research and teaching experience, Eaton E. Lattman and Patrick J. Loll use clear examples and abundant illustrations to provide a concise and accessible primer on protein crystallography. Discussing the basics of diffraction, the behavior of two- and three-dimensional crystals, phase determination (including MIR and MAD phasing and molecular replacement), the Patterson function, and refinement, Lattman and Loll provide a complete overview of this important technique, illuminated by physical insights. The crisp writing style and simple illustrations will provide beginner crystallographers with a guide to the process of unraveling protein structure.

Author : Duncan E. McRee
Publisher : Academic Press
Page : 386 pages
File Size : 51,6 Mb
Release : 2012-12-02
Category : Science
ISBN : 9780323138888

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Practical Protein Crystallography by Duncan E. McRee Pdf

Designed for easy use by both new and experienced protein crystallographers, this much-needed book is for anyone interested in solving protein structures by the method of crystallography. It contains many examples ofactual experiments and data, including electron density maps. Computer methods and computer code samples are presented. Practical Protein Crystallography is loaded with new information on area detectors, synchrotron radiation techniques, and the latest computer methods, and features the XtalView software system. Graduate students and teachers in physical biochemistry and pharmaceutical researchers will find this text a timely and convenient aid.

Author : Alexander Wlodawer,Zbigniew Dauter,Mariusz Jaskolski
Publisher : Humana Press
Page : 672 pages
File Size : 55,7 Mb
Release : 2017-06-02
Category : Science
ISBN : 1493969986

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Protein Crystallography by Alexander Wlodawer,Zbigniew Dauter,Mariusz Jaskolski Pdf

This volume provides methods for modern macromolecular crystallography, including all steps leading to crystal structure determination and analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Protein Crystallography aims to ensure successful results in the further study of this vital field.

Author : Robert E. Babine,Sherin S. Abdel-Meguid
Publisher : John Wiley & Sons
Page : 278 pages
File Size : 44,9 Mb
Release : 2008-07-15
Category : Science
ISBN : 9783527606351

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Protein Crystallography in Drug Discovery by Robert E. Babine,Sherin S. Abdel-Meguid Pdf

The rational, structure-based approach has become standard in present-day drug design. As a consequence, the availability of high-resolution structures of target proteins is more often than not the basis for an entire drug development program. Protein structures suited for rational drug design are almost exclusively derived from crystallographic studies, and drug developers are relying heavily on the power of this method. Here, researchers from leading pharmaceutical companies present valuable first-hand information, much of it published for the first time. They discuss strategies to derive high-resolution structures for such important target protein classes as kinases or proteases, as well as selected examples of successful protein crystallographic studies. A special section on recent methodological developments, such as for high-throughput crystallography and microcrystallization, is also included. A valuable companion for crystallographers involved in protein structure determination as well as drug developers pursuing the structure-based approach for use in their daily work.

Author : T. L. Blundell,L. N. Johnson
Publisher : Academic Press
Page : 592 pages
File Size : 42,9 Mb
Release : 1976-01-28
Category : Science
ISBN : UCLA:L0065432452

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Protein Crystallography by T. L. Blundell,L. N. Johnson Pdf

Author : Gwyndaf Evans,Konstantinos Beis
Publisher : Royal Society of Chemistry
Page : 244 pages
File Size : 55,9 Mb
Release : 2018-06-21
Category : Science
ISBN : 9781782627289

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Protein Crystallography by Gwyndaf Evans,Konstantinos Beis Pdf

Protein crystallography has become vital to further understanding the structure and function of many complex biological systems. In recent years, structure determination has progressed tremendously however the quality of crystals and data sets can prevent the best results from being obtained. With contributions from world leading researchers whose software are used worldwide, this book provides a coherent approach on how to handle difficult crystallographic data and how to assess its quality. The chapters will cover all key aspects of protein crystallography, from instrumentation and data processing through to model building. This book also addresses challenges that protein crystallographers will face such as dealing with data from microcrystals and multi protein complexes. This book is ideal for both academics and researchers in industry looking for a comprehensive guide to protein crystallography.

Author : Nobuo Niimura,Alberto Podjarny
Publisher : Oxford University Press
Page : 250 pages
File Size : 47,9 Mb
Release : 2011-02-17
Category : Science
ISBN : 9780191004810

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Neutron Protein Crystallography by Nobuo Niimura,Alberto Podjarny Pdf

This is one of the first books dedicated to the emerging field of neutron protein crystallography (NPC). The text covers all of the practical aspects of NPC, from the basic background of neutron scattering and diffraction, to the technical details of neutron facilities, growth of high-quality crystals, and data analysis. The final chapter is devoted to providing many examples of using NPC to investigate a wide range of different proteins. It demonstrates how NPC can explore hydrogen bonds, protonation and deprotonation of amino acid residues, hydration structures, and hydrogen-to-deuterium exchange ratios. To avoid redundancy with other textbooks on X-ray protein crystallography (XPC), this book assumes a familiarity with the basics of XPC and strives to highlight and explain the differences between XPC and NPC. It is therefore especially useful for X-ray protein crystallographers who are eager to have a sound, scientific basis for judging if NPC is the right technique for furthering their experimental programs.

Author : Jan Drenth
Publisher : Springer Science & Business Media
Page : 355 pages
File Size : 46,7 Mb
Release : 2013-03-09
Category : Science
ISBN : 9781475730920

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Principles of Protein X-ray Crystallography by Jan Drenth Pdf

New textbooks at all levels of chemistry appear with great regularity. Some fields such as basic biochemistry, organic reaction mechanisms, and chemical thermodynamics are well represented by many excellent texts, and new or revised editions are published sufficiently often to keep up with progress in research. However, some areas of chemistry, especially many of those taught at the graduate level, suffer from a real lack of up to-date textbooks. The most serious needs occur in fields that are rapidly changing. Textbooks in these subjects usually have to be written by scientists actually involved in the research that is advancing the field. It is not often easy to persuade such individuals to set time aside to help spread the knowledge they have accumulated. Our goal, in this series, is to pinpoint areas of chemistry where recent progress has outpaced what is covered in any available textbooks, and then seek out and persuade experts in these fields to produce relatively concise but instructive intro ductions to their fields. These should serve the needs of one-semester or one-quarter graduate courses in chemistry and biochemistry. In some cases, the availability of texts in active research areas should help stimulate the creation of new courses. Charles R. Cantor v Preface to the Second Edition Since the publication of the previous edition in 1994, X-ray crystallography of proteins has advanced by improvements in existing techniques and by addition of new techniques.

Author : Anonim
Publisher : Elsevier
Page : 404 pages
File Size : 50,8 Mb
Release : 2023-09-25
Category : Science
ISBN : 9780443159275

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Crystallography of Protein Dynamics by Anonim Pdf

Approx.400 pages Approx.400 pages

Author : Robert E. Babine,Sherin S. Abdel-Meguid
Publisher : John Wiley & Sons
Page : 284 pages
File Size : 48,7 Mb
Release : 2004-02-13
Category : Medical
ISBN : 3527306781

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Protein Crystallography in Drug Discovery, Volume 20 by Robert E. Babine,Sherin S. Abdel-Meguid Pdf

The rational, structure-based approach has become standard in present-day drug design. As a consequence, the availability of high-resolution structures of target proteins is more often than not the basis for an entire drug development program. Protein structures suited for rational drug design are almost exclusively derived from crystallographic studies, and drug developers are relying heavily on the power of this method. Here, researchers from leading pharmaceutical companies present valuable first-hand information, much of it published for the first time. They discuss strategies to derive high-resolution structures for such important target protein classes as kinases or proteases, as well as selected examples of successful protein crystallographic studies. A special section on recent methodological developments, such as for high-throughput crystallography and microcrystallization, is also included. A valuable companion for crystallographers involved in protein structure determination as well as drug developers pursuing the structure-based approach for use in their daily work.

Author : Isabel Moraes
Publisher : Springer
Page : 184 pages
File Size : 42,9 Mb
Release : 2016-08-23
Category : Science
ISBN : 9783319350721

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The Next Generation in Membrane Protein Structure Determination by Isabel Moraes Pdf

This book reviews current techniques used in membrane protein structural biology, with a strong focus on practical issues. The study of membrane protein structures not only provides a basic understanding of life at the molecular level but also helps in the rational and targeted design of new drugs with reduced side effects. Today, about 60% of the commercially available drugs target membrane proteins and it is estimated that nearly 30% of proteins encoded in the human genome are membrane proteins. In recent years much effort has been put towards innovative developments to overcome the numerous obstacles associated with the structure determination of membrane proteins. This book reviews a variety of recent techniques that are essential to any modern researcher in the field of membrane protein structural biology. The topics that are discussed are not commonly found in textbooks. The scope of this book includes: Expression screening using fluorescent proteins The use of detergents in membrane protein research The use of NMR Synchrotron developments in membrane protein structural biology Visualisation and X-ray data collection of microcrystals X-ray diffraction data analysis from multiple crystals Serial millisecond crystallography Serial femtosecond crystallography Membrane protein structures in drug discovery The information provided in this book should be of interest to anyone working in the area of structural biology. Students will find carefully prepared overviews of basic ideas and advanced protein scientists will find the level of detail required to apply the material directly to their day to day work. Chapters 4, 5, 6, 8 and 9 of this book are published open access under a CC BY 4.0 license at link.springer.com.

Author : Anonim
Publisher : Academic Press
Page : 336 pages
File Size : 51,8 Mb
Release : 2009-05-29
Category : Science
ISBN : 0080961592

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Membrane Protein Crystallization by Anonim Pdf

This volume of Current Topics in Membranes focuses on Membrane Protein Crystallization, beginning with a review of past successes and general trends, then further discussing challenges of mebranes protein crystallization, cell free production of membrane proteins and novel lipids for membrane protein crystallization. This publication also includes tools to enchance membrane protein crystallization, technique advancements, and crystallization strategies used for photosystem I and its complexes, establishing Membrane Protein Crystallization as a needed, practical reference for researchers.

Author : Terese M. Bergfors
Publisher : Internat'l University Line
Page : 505 pages
File Size : 40,5 Mb
Release : 2009
Category : Medical
ISBN : 9780972077446

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Protein Crystallization by Terese M. Bergfors Pdf

Author : Marc L. Pusey,Ramazan Savaş Aygün
Publisher : Springer
Page : 245 pages
File Size : 44,5 Mb
Release : 2017-11-27
Category : Computers
ISBN : 9783319589374

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Data Analytics for Protein Crystallization by Marc L. Pusey,Ramazan Savaş Aygün Pdf

This unique text/reference presents an overview of the computational aspects of protein crystallization, describing how to build robotic high-throughput and crystallization analysis systems. The coverage encompasses the complete data analysis cycle, including the set-up of screens by analyzing prior crystallization trials, the classification of crystallization trial images by effective feature extraction, the analysis of crystal growth in time series images, the segmentation of crystal regions in images, the application of focal stacking methods for crystallization images, and the visualization of trials. Topics and features: describes the fundamentals of protein crystallization, and the scoring and categorization of crystallization image trials; introduces a selection of computational methods for protein crystallization screening, and the hardware and software architecture for a basic high-throughput system; presents an overview of the image features used in protein crystallization classification, and a spatio-temporal analysis of protein crystal growth; examines focal stacking techniques to avoid blurred crystallization images, and different thresholding methods for binarization or segmentation; discusses visualization methods and software for protein crystallization analysis, and reviews alternative methods to X-ray diffraction for obtaining structural information; provides an overview of the current challenges and potential future trends in protein crystallization. This interdisciplinary work serves as an essential reference on the computational and data analytics components of protein crystallization for the structural biology community, in addition to computer scientists wishing to enter the field of protein crystallization.