Protein Kinase Protocols

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Protein Kinase Protocols

Alastair D. Reith
Protein Kinase Protocols Book PDF

Author : Alastair D. Reith
Publisher : Springer Science & Business Media
Page : 367 pages
File Size : 43,6 Mb
Release : 2008-02-02
Category : Science
ISBN : 9781592590599

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Protein Kinase Protocols by Alastair D. Reith Pdf

As key components of many cell signaling pathways, protein kinases are implicated in a broad variety of diseases, including cancers and neurodegenerative conditions, and offer considerable potential as tractable targets for therapeutic intervention. In Protein Kinase Protocols, a panel of highly skilled laboratory investigators describe both basic and more sophisticated methods for the analysis of kinase-mediated signaling cascades, with emphasis on the identification of proteins according to their interactive relationships and the analysis of their functional properties. Described in step-by-step detail, these readily reproducible techniques offer novices quick access to a complicated field, and provide more experienced investigators many novel time-saving ploys. Emphasis is given to the critical technical steps that are often omitted from methods published in the primary literature. There are also tips on potential pitfalls and copious notes on how to adjust the protocols to work in related systems. Broad in its range of techniques and thoroughly detailed to help ensure experimental success, Protein Kinase Protocols offers both novice and experienced investigators powerful tools for understanding the functional roles of specific protein kinases within signaling cascades and for identification and evaluation of novel therapeutic targets.

Protein Kinase C Protocols

Alexandra C. Newton
Protein Kinase C Protocols Book PDF

Author : Alexandra C. Newton
Publisher : Springer Science & Business Media
Page : 565 pages
File Size : 42,5 Mb
Release : 2008-02-03
Category : Science
ISBN : 9781592593972

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Protein Kinase C Protocols by Alexandra C. Newton Pdf

Since the discovery that protein kinase C (PKC) transduces the ab- dance of signals that result in phospholipid hydrolysis, this enzyme has been at the forefront of research in signal transduction. Protein Kinase C Protocols covers fundamental methods for studying the structure, function, regulation, subcellular localization, and macromolecular interactions of PKC. Protein Kinase C Protocols is divided into 11 sections representing the major aspects of PKC regulation and function. Part I contains an introduction and a historical perspective on the discovery of PKC by Drs. Yasutomi Nishizuka and Ushio Kikkawa. Part II describes methods to purify PKC. Part III describes the standard methods for measuring PKC activity: its enzymatic activity and its stimulus-dependent translocation from the cytosol to the membrane. Part IV describes methods for measuring the membrane interaction of PKC in vivo and in vitro. Part V provides methodologies and techniques for measuring the ph- phorylation state of PKC, including a protocol for measuring the activity of PKC’s upstream kinase, PDK-1. Novel methods for identifying substrates are described in Part VI. Part VII presents protocols for expressing and analyzing the membrane targeting domains of PKC. Part VIII provides a comprehensive c- pilation of methods used to identify binding partners for PKC. Part IX describes pharmacological probes used to study PKC. The book ends with a presentation of genetic approaches to study PKC (Part X) and a discussion of approaches used to study PKC in disease (Part XI).

MAP Kinase Signaling Protocols

Rony Seger
MAP Kinase Signaling Protocols Book PDF

Author : Rony Seger
Publisher : Springer Science & Business Media
Page : 335 pages
File Size : 43,9 Mb
Release : 2008-02-03
Category : Science
ISBN : 9781592596713

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MAP Kinase Signaling Protocols by Rony Seger Pdf

Mitogen-activated protein kinase (MAPK) signaling cascades are a group of protein kinases that play a central role in the intracellular transmission of extracellular signals. These cascades operate as major lines of communication within a complicated signaling network that regulates many cellular processes, including proliferation, differentiation, development, stress response, and apoptosis. More than 15,000 papers on MAPKs have been published over the past few years, with the number of publications increasing each year. More and more laboratories embark on the study of MAPK cascades in many d- tinct cellular systems and in particular their role in disease. Future challenges in the study of MAPK cascades remain in understa- ing the role of the various components and isoforms of the cascades in the multiple critical functions that they regulate in the whole organism, as well as the diseases caused by their malfunction. Data from gene-disrupted mice s- gest that inhibition of the MAPK cascades may have serious consequences on the development and growth of the animals. For example, targeted deletion of MEK1 is lethal, owing to developmental problems of placental vasculature and abnormal fibroblast migration. This lethality occurs in spite of the normal expression of MEK2, indicating that although the two MEK isoforms are apparently similar, they do have distinct functions, at least during embryog- esis. The ERK cascade was also shown to play a central role in brain function and in learning and memory.

Protein Targeting Protocols

Roger A. Clegg
Protein Targeting Protocols Book PDF

Author : Roger A. Clegg
Publisher : Springer Science & Business Media
Page : 331 pages
File Size : 45,5 Mb
Release : 2008-02-04
Category : Science
ISBN : 9781592595723

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Protein Targeting Protocols by Roger A. Clegg Pdf

It is by no means a revelation that proteins are not uniformly distributed throughout the cell. As a result, the idea that protein molecules, because of the specificity with which they can engage in interactions with other proteins, may be aimed—via these interactions—at a restricted target, is a fundamental one in contemporary molecular life sciences. The target may be variously c- ceived as a specific molecule, a group of molecules, a structure, or a more generic type of intracellular environment. Because the concept of protein targeting is intuitive rather than expl- itly defined, it has been variously used by different groups of researchers in cell biology, biochemistry, and molecular biology. For those working in the field of intracellular signaling, an influential introduction to the topic was the seminal article by Hubbard & Cohen (TIBS [1993] 18, 172–177), which was based on the work of Cohen’s laboratory on protein phosphatases. Sub- quently, the ideas that they discussed have been further developed and extended by many workers to other key intermediaries in intracellular sign- ing, including protein kinases and a great variety of modulator and adaptor proteins.

Receptor Tyrosine Kinases

Serena Germano
Receptor Tyrosine Kinases Book PDF

Author : Serena Germano
Publisher : Humana
Page : 0 pages
File Size : 40,8 Mb
Release : 2016-09-22
Category : Science
ISBN : 1493954490

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Receptor Tyrosine Kinases by Serena Germano Pdf

Receptor Tyrosine Kinases: Methods and Protocols explores on the most widely studied of the protein classes, known for their central role in several cellular functions and in a variety of human pathologies. Beginning with a section on activation and signaling of Receptor Tyrosine Kinases (RTKs), the book continues with chapters on aberrant expression of RTKs, trafficking and negative regulation, as well as RTKs as pharmacological targets. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Receptor Tyrosine Kinases: Methods and Protocols contains an up-to-date overview of the most relevant and widely used methods employed in this field, providing detailed protocols that molecular and cellular biologists could easily adopt in their research programs on RTKs.

Plant Kinases

Nico Dissmeyer,Arp Schnittger
Plant Kinases Book PDF

Author : Nico Dissmeyer,Arp Schnittger
Publisher : Humana Press
Page : 312 pages
File Size : 49,5 Mb
Release : 2016-08-23
Category : Science
ISBN : 149395721X

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Plant Kinases by Nico Dissmeyer,Arp Schnittger Pdf

Modification of target protein properties by reversible phosphorylation events has been found to be one of the most prominent cellular control processes in all organisms. Recent advances in the areas of molecular biology and biochemistry are presenting new possibilities for reaching an unprecedented depth and a proteome-wide understanding of phosphorylation processes in plants as well as in other species. The major goal of Plant Kinases: Methods and Protocols is to provide the experimentalist with a detailed account of the practical steps necessary for successfully carrying out each protocol in his or her own laboratory. Plant protein kinases specifically addressed in this volume are members of the plant MAP kinase cascade, cyclin- and Calcium-dependent protein kinases, and plant sensor and receptor kinases. Written in the highly successful Methods in Molecular BiologyTM series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Plant Kinases: Methods and Protocols will prove a useful laboratory companion to both novice and seasoned researchers by facilitating the practical work that will lead them to new and exciting insights in this dynamic field.

Kinase Screening and Profiling

Hicham Zegzouti,Said A. Goueli
Kinase Screening and Profiling Book PDF

Author : Hicham Zegzouti,Said A. Goueli
Publisher : Humana Press
Page : 223 pages
File Size : 55,6 Mb
Release : 2016-08-23
Category : Science
ISBN : 1493949233

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Kinase Screening and Profiling by Hicham Zegzouti,Said A. Goueli Pdf

This volume focuses on screening and profiling kinase inhibitors or activators. Chapters in this book cover several facets of the drug discovery processes starting with target identification, and assay development and screening chemical libraries for hit identification and lead optimization. The profiling portion of Kinase Screening and Profiling: Methods and Protocols discusses several strategies and techniques that are required to minimize off target hits and reduce cellular toxicities that are caused by liability kinases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Timely and authoritative, Kinase Screening and Profiling: Methods and Protocols, will benefit scientists and researchers who are interested in exploring the role of kinases in academic, as well as pharmaceutical research.

Signal Transduction Protocols

Robert C. Dickson,Michael D. Mendenhall
Signal Transduction Protocols Book PDF

Author : Robert C. Dickson,Michael D. Mendenhall
Publisher : Springer Science & Business Media
Page : 331 pages
File Size : 46,8 Mb
Release : 2008-02-02
Category : Science
ISBN : 9781592598168

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Signal Transduction Protocols by Robert C. Dickson,Michael D. Mendenhall Pdf

In 1995, Signal Transduction Protocols, edited by David A. Kendall and Stephen J. Hill, was published in the Methods in Molecular Biology series. This second edition represents an update to that previous work with an emp- sis on new methodologies that have developed in the last few years. The goal, then and now, is to provide procedures written by experts with first-hand ex- rience in a detail that goes far beyond what is generally encountered in the “methods” section of most journals and thus actually permits a particular p- cedure to be replicated. In addition, we have had as a secondary goal the id- tification of protocols for the assay of general classes of signal transduction components that, ideally, can be adapted to the assay of any member of that class. The ability to do this has resulted in large part from the use of affini- based assays, the ease with which specific proteins can be specifically tagged, and an explosion in the availability of highly specific antibodies from comm- cial sources, especially antibodies raised against signaling proteins of human origin. The number of available approaches is, fortunately for those working in signaling research, far too great to fit within the confines of this volume, so hard choices as to what to include had to be made.

Kinase Inhibitors

Bernhard Kuster
Kinase Inhibitors Book PDF

Author : Bernhard Kuster
Publisher : Humana Press
Page : 256 pages
File Size : 41,6 Mb
Release : 2011-10-06
Category : Medical
ISBN : 1617793361

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Kinase Inhibitors by Bernhard Kuster Pdf

Protein and lipid kinases are often the master regulators of cell signaling in eukaryotic systems. The human genome codes for more than 500 of these enzymes and their misregulation has been shown to be involved in the onset and progression of many diseases including cancer and inflammation. Therefore, small molecule kinase inhibitors have become important research tools for the elucidation of the many biological roles of kinases and their mechanisms of action. Kinase inhibitors thus also contribute significantly to the drug pipelines of the pharmaceutical and biotechnology industries and to the growing need to treat cancer and inflammation. In Kinase Inhibitors: Methods and Protocols, experts in kinase biology, drug discovery, and clinical research present a series of exemplary methods that can be used to address the many challenges facing scientists in the discovery and development of kinase inhibitors both for research and clinical use. Written in the highly successful Methods in Molecular BiologyTM series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Kinase Inhibitors: Methods and Protocols aims to provide scientists with modern and relevant methods to accelerate or strengthen their research and drug discovery programs through the utilization of these vital regulators.

Transmembrane Signaling Protocols

Dafna Bar-Sagi
Transmembrane Signaling Protocols Book PDF

Author : Dafna Bar-Sagi
Publisher : Springer Science & Business Media
Page : 631 pages
File Size : 49,5 Mb
Release : 1998
Category : Cell membranes
ISBN : 9780896034884

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Transmembrane Signaling Protocols by Dafna Bar-Sagi Pdf

This collection of practical, cutting-edge techniques for the study of cell signaling provides detailed, step-by-step instructions, helpful notes, and troubleshooting tips that make even the most powerful of the newest techniques readily reproducible. The protocols presented include the use of peptide libraries to study transmembrane signaling; the use of single-cell assays to analyze signal transduction pathways; the reconstitution of signaling complexes; methods for analyzing protein-protein interactions, and more. Introductory reviews explain the basic theory and enable researchers new to the area to rapidly gain understanding, as well as command of the practical knowledge and expertise afforded by the protocols. Transmembrane Signaling Protocols makes available to all researchers the many state-of-the-art techniques that have recently led to landmark discoveries in transmembrane signaling.

Protein Phosphatase Protocols

Greg Moorhead
Protein Phosphatase Protocols Book PDF

Author : Greg Moorhead
Publisher : Springer Science & Business Media
Page : 390 pages
File Size : 44,9 Mb
Release : 2008-02-05
Category : Science
ISBN : 9781597452670

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Protein Phosphatase Protocols by Greg Moorhead Pdf

Protein Phosphatase Protocols presents a broad range of protocols for the study of protein phosphatases, all written by experts and innovators from phosphatase laboratories around the world. This volume is a compendium of resources for the study of protein phosphatases and their potential as drug targets. Experimental methodologies are taken from proteomics, bioinformatics, genomics, biochemistry, RNAi, and genetics.

Jak-Stat Signalling

Sandra E. Nicholson,Nicos Anthony Nicola
Jak Stat Signalling Book PDF

Author : Sandra E. Nicholson,Nicos Anthony Nicola
Publisher : Humana Press
Page : 334 pages
File Size : 46,9 Mb
Release : 2016-05-01
Category : Science
ISBN : 1493959794

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Jak-Stat Signalling by Sandra E. Nicholson,Nicos Anthony Nicola Pdf

This Methods in Molecular Biology volume covers JAK and STAT specific approaches, the negative regulators of the pathway, and production and crystallization of JAK and STAT proteins, and more.Includes protocols, materials lists, tips and troubleshooting advice."

Protein Kinase Functions

James Woodgett
Protein Kinase Functions Book PDF

Author : James Woodgett
Publisher : OUP Oxford
Page : 407 pages
File Size : 41,8 Mb
Release : 2000-08-24
Category : Science
ISBN : 9780191565960

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Protein Kinase Functions by James Woodgett Pdf

Since the publication of Protein Kinases in 1994 many novel protein kinases have been discovered, but perhaps more importantly there have been dramatic advances in our understanding of the cellular functions of this remarkably diverse class of proteins. Protein Kinase Functions is not just an update of the previous edition but provides a new focus on the context and function of protein kinases, thus reflecting the recent advances in kinase biology. Chapters on genetic approaches to protein kinase functions, the MAP kinase pathway, and cyclin-dependent kinases have been completely updated and new topics covered in depth are: phosphatidylinositol 3-kinase signalling, JAK-STAT signalling, suppression of tyrosine kinases by the SOCS family proteins, the TGFß superfamily, and the involvement of protein kinases in response to DNA damage. Throughout, emphasis is placed not on individual kinases, but on the functional aspects of the whole system and the relationship between processes and molecules. It is the aim of Protein Kinase Functions to enable the reader to assimilate, compare, and integrate the molecular machinery used by cells to co-ordinate and respond to their environments.

Plant MAP Kinases

George Komis,Jozef Šamaj
Plant MAP Kinases Book PDF

Author : George Komis,Jozef Šamaj
Publisher : Humana
Page : 0 pages
File Size : 55,8 Mb
Release : 2016-09-17
Category : Science
ISBN : 1493945890

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Plant MAP Kinases by George Komis,Jozef Šamaj Pdf

Mitogen-activated protein kinases (MAPKs) are versatile phosphorylating enzymes which regulate multiple proteins involved in gene expression, cell architecture, plant development and reaction to diverse abiotic and biotic factors. The main aim of Plant MAP Kinases: Methods and Protocols is to provide established and new MAPK protocols adapted to the challenges posed by working with plants. The book contains 19 chapters which encompass a wide array of methods progressively scaling from the single gene, protein or cell level to large-scale arrays of proteomic, phosphoproteomic and interactomic data in order to uncover previously unidentified plant MAPK signaling pathways and to tackle with the challenging task of substrate identification. Techniques for MAPK sequence analysis and subcellular localization helping to identify their substrates and subcellular compartmentalization are also provided. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant MAP Kinases: Methods and Protocols represents a collection of useful plant MAPK protocols written by experts in the field for researchers and students.

The Protein Protocols Handbook

John M. Walker
The Protein Protocols Handbook Book PDF

Author : John M. Walker
Publisher : Springer Science & Business Media
Page : 759 pages
File Size : 48,8 Mb
Release : 1996
Category : Electronic books
ISBN : 9780896033382

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The Protein Protocols Handbook by John M. Walker Pdf

-- 145 concise, step-by-step protocols for the analysis of proteins and peptides -- Provides more than three times as many protocols than the competitors' manuals -- in the incomparable Methods in Molecular Biology "TM" style -- at an affordable price -- Combound in a large 7 x l0 inch format for easy benchtop use -- An indispensable reference for industry-based researchers, professors, and their students