Protein Ligand Interactions

Author : Mark A. Williams,Tina Daviter
Publisher : Humana
Page : 0 pages
File Size : 45,9 Mb
Release : 2016-11-17
Category : Science
ISBN : 1493958739

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Protein-Ligand Interactions by Mark A. Williams,Tina Daviter Pdf

Proteins are the cell’s workers, their messengers and overseers. In these roles, proteins specifically bind small molecules, nucleic acid and other protein partners. Cellular systems are closely regulated and biologically significant changes in populations of particular protein complexes correspond to very small variations of their thermodynamics or kinetics of reaction. Interfering with the interactions of proteins is the dominant strategy in the development of new pharmaceuticals. Protein Ligand Interactions: Methods and Applications, Second Edition provides a complete introduction to common and emerging procedures for characterizing the interactions of individual proteins. From the initial discovery of natural substrates or potential drug leads, to the detailed quantitative understanding of the mechanism of interaction, all stages of the research process are covered with a focus on those techniques that are, or are anticipated to become, widely accessible and performable with mainstream commercial instrumentation. Written in the highly successful Methods in Molecular Biology series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Protein Ligand Interactions: Methods and Applications, Second Edition serves as an ideal guide for researchers new to the field of biophysical characterization of protein interactions – whether they are beginning graduate students or experts in allied areas of molecular cell biology, microbiology, pharmacology, medicinal chemistry or structural biology.

Author : Holger Gohlke
Publisher : John Wiley & Sons
Page : 361 pages
File Size : 46,5 Mb
Release : 2012-05-21
Category : Medical
ISBN : 9783527329663

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Protein-Ligand Interactions by Holger Gohlke Pdf

Innovative and forward-looking, this volume focuses on recent achievements in this rapidly progressing field and looks at future potential for development. The first part provides a basic understanding of the factors governing protein-ligand interactions, followed by a comparison of key experimental methods (calorimetry, surface plasmon resonance, NMR) used in generating interaction data. The second half of the book is devoted to insilico methods of modeling and predicting molecular recognition and binding, ranging from first principles-based to approximate ones. Here, as elsewhere in the book, emphasis is placed on novel approaches and recent improvements to established methods. The final part looks at unresolved challenges, and the strategies to address them. With the content relevant for all drug classes and therapeutic fields, this is an inspiring and often-consulted guide to the complexity of protein-ligand interaction modeling and analysis for both novices and experts.

Author : Hans-Joachim Böhm,Gisbert Schneider
Publisher : John Wiley & Sons
Page : 262 pages
File Size : 46,5 Mb
Release : 2006-03-06
Category : Science
ISBN : 9783527605514

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Protein-Ligand Interactions by Hans-Joachim Böhm,Gisbert Schneider Pdf

The lock-and-key principle formulated by Emil Fischer as early as the end of the 19th century has still not lost any of its significance for the life sciences. The basic aspects of ligand-protein interaction may be summarized under the term 'molecular recognition' and concern the specificity as well as stability of ligand binding. Molecular recognition is thus a central topic in the development of active substances, since stability and specificity determine whether a substance can be used as a drug. Nowadays, computer-aided prediction and intelligent molecular design make a large contribution to the constant search for, e. g., improved enzyme inhibitors, and new concepts such as that of pharmacophores are being developed. An up-to-date presentation of an eternally young topic, this book is an indispensable information source for chemists, biochemists and pharmacologists dealing with the binding of ligands to proteins.

Author : Stephen E. Harding,Babur Z. Chowdhry
Publisher : Oxford University Press, USA
Page : 474 pages
File Size : 52,5 Mb
Release : 2001
Category : Medical
ISBN : 0199637474

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Protein-ligand Interactions, Structure and Spectroscopy by Stephen E. Harding,Babur Z. Chowdhry Pdf

This text on protein-ligand interactions offers a selection of the most useful and easily applied methods and acts as a guide to the principal techniques used.

Author : Stephen E. Harding,Babur Z. Chowdhry
Publisher : Unknown
Page : 128 pages
File Size : 45,7 Mb
Release : 2001
Category : Electronic
ISBN : 0199637466

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Protein-ligand Interactions by Stephen E. Harding,Babur Z. Chowdhry Pdf

Author : Flavio Ballante
Publisher : Unknown
Page : 327 pages
File Size : 47,5 Mb
Release : 2021
Category : Cheminformatics
ISBN : 1071612093

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Protein-Ligand Interactions and Drug Design by Flavio Ballante Pdf

This detailed book collects modern and established computer-based methods aimed at addressing the drug discovery challenge from disparate perspectives by exploiting information on ligand-protein recognition. Beginning with methods that allow for the exploration of specific areas of chemical space and the designing of virtual libraries, the volume continues with sections on methods based on docking, quantitative models, and molecular dynamics simulations, which are employed for ligand discovery or development, as well as methods exploiting an ensemble of protein structures for the identification of potential protein targets. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Protein-Ligand Interactions and Drug Design provides detailed practical procedures of solid computer-aided drug design methodologies employed to rationalize and optimize protein-ligand interactions, for experienced researchers and novices alike.

Author : G. Ulrich Nienhaus
Publisher : Humana Press
Page : 568 pages
File Size : 48,7 Mb
Release : 2005-03-21
Category : Science
ISBN : 1588293726

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Protein'Ligand Interactions by G. Ulrich Nienhaus Pdf

A readily reproducible collection of established and emerging techniques for studying the interaction between proteins and ligands, including biochemical/bulk techniques, structure analysis, spectroscopy, single-molecule studies, and theoretical/computational tools. Among the highlights are surface plasmon resonance (SPR) and reflectometric biosensor approaches, high-throughput screening with confocal optics microscopy, single molecule fluorescence and fluorescence correlation spectroscopy (FCS), atomic force microscopy (AFM), crystallography of reaction intermediates, and time-resolved x-ray crystallography. The protocols follow the successful Methods in Molecular BiologyTM series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

Author : Tina Daviter,Christopher Johnson,Stephen McLaughlin,Mark Williams
Publisher : Humana
Page : 496 pages
File Size : 54,6 Mb
Release : 2021-04-19
Category : Science
ISBN : 1071611968

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Protein-Ligand Interactions by Tina Daviter,Christopher Johnson,Stephen McLaughlin,Mark Williams Pdf

This third edition provides new and updated chapters detailing a complete introduction to common and emerging procedures for characterizing the interactions of individual proteins with their natural ligands, drugs or other binding partners. Chapters detail natural substrates, potential drug leads, quantitative understanding of the mechanism of interaction, and different techniques. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Protein Ligand Interactions: Methods and Applications, Third Edition serves as an ideal guide for researchers new to the field of biophysical characterization of protein interactions.

Author : Horst Sund,Gideon Blauer
Publisher : Walter de Gruyter GmbH & Co KG
Page : 512 pages
File Size : 51,6 Mb
Release : 2019-03-18
Category : Science
ISBN : 9783110830811

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Protein-Ligand Interactions by Horst Sund,Gideon Blauer Pdf

Keine ausführliche Beschreibung für "Protein-Ligand Interactions" verfügbar.

Author : Holger Gohlke
Publisher : John Wiley & Sons
Page : 359 pages
File Size : 47,6 Mb
Release : 2012-04-06
Category : Medical
ISBN : 9783527645978

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Protein-Ligand Interactions by Holger Gohlke Pdf

Innovative and forward-looking, this volume focuses on recent achievements in this rapidly progressing field and looks at future potential for development. The first part provides a basic understanding of the factors governing protein-ligand interactions, followed by a comparison of key experimental methods (calorimetry, surface plasmon resonance, NMR) used in generating interaction data. The second half of the book is devoted to insilico methods of modeling and predicting molecular recognition and binding, ranging from first principles-based to approximate ones. Here, as elsewhere in the book, emphasis is placed on novel approaches and recent improvements to established methods. The final part looks at unresolved challenges, and the strategies to address them. With the content relevant for all drug classes and therapeutic fields, this is an inspiring and often-consulted guide to the complexity of protein-ligand interaction modeling and analysis for both novices and experts.

Author : Thomas Peters
Publisher : Springer
Page : 309 pages
File Size : 44,8 Mb
Release : 2006-12-08
Category : Science
ISBN : 9783540490784

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Bioactive Conformation I by Thomas Peters Pdf

This series presents critical reviews of the present position and future trends in modern chemical research. It contains short and concise reports on chemistry, each written by the world renowned experts – it is still valid and useful after 5 or 10 years. More information as well as the electronic version of the whole content available at: springerlink.com. The book will appeal to scientists and practitioners in the mentioned fields and in industry.

Author : W. Guschlbauer
Publisher : Springer Science & Business Media
Page : 289 pages
File Size : 55,9 Mb
Release : 2013-06-29
Category : Science
ISBN : 9781468453836

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DNA-Ligand Interactions by W. Guschlbauer Pdf

This volume contains the texts of the nineteen lectures presented at the NATO-ASI - FEBS Course on "DNA - ligand interactions: from drugs to proteins." The Advanced Study Institute (ASIl was held from August 30th to September 11th. 1986 in the Abbey of Fontevraud (France). The ASI was attended by 112 participants from a wide scientific horizon and from twentyone different countries. It was in some way a follow-up of the ASI held in Maratea. Italy in May 1981 and which was published in the NATO ASI Life Science series as volume 45. While much has been learned about the way the cellular machinery maintains and transmits the genetic heritage. as well as how these processes are regulated. little is Known about how the interactions between the various partners involved are taKing place. The interactions of drugs and proteins with nucleic acids are of evident importance in the understanding of these problems. The spectacular advances in recombinant DNA technology and the increased sophistication of biophysical techniques. in particular >:-ray diffraction and nuclear magnetic resonance. have created a scientific environment which is highly promising for the future of research in molecular biology. These advances permH the serious hope that biology on the molecular level may become a r-eality. Some of the contributions at the ASI presented the most recent advances in this e>:citing field.

Author : Donald Huddler,Edward R. Zartler
Publisher : John Wiley & Sons
Page : 148 pages
File Size : 42,6 Mb
Release : 2017-10-02
Category : Science
ISBN : 9781119099482

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Applied Biophysics for Drug Discovery by Donald Huddler,Edward R. Zartler Pdf

Applied Biophysics for Drug Discovery is a guide to new techniques and approaches to identifying and characterizing small molecules in early drug discovery. Biophysical methods are reasserting their utility in drug discovery and through a combination of the rise of fragment-based drug discovery and an increased focus on more nuanced characterisation of small molecule binding, these methods are playing an increasing role in discovery campaigns. This text emphasizes practical considerations for selecting and deploying core biophysical method, including but not limited to ITC, SPR, and both ligand-detected and protein-detected NMR. Topics covered include: • Design considerations in biophysical-based lead screening • Thermodynamic characterization of protein-compound interactions • Characterizing targets and screening reagents with HDX-MS • Microscale thermophoresis methods (MST) • Screening with Weak Affinity Chromatography • Methods to assess compound residence time • 1D-NMR methods for hit identification • Protein-based NMR methods for SAR development • Industry case studies integrating multiple biophysical methods This text is ideal for academic investigators and industry scientists planning hit characterization campaigns or designing and optimizing screening strategies.

Author : Mendel Friedman
Publisher : Springer Science & Business Media
Page : 686 pages
File Size : 40,9 Mb
Release : 2012-12-06
Category : Science
ISBN : 9781468409437

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Protein-Metal Interactions by Mendel Friedman Pdf

Metal ions and proteins are ubiquitous. Therefore, not surprisingly, new protein-metal interactions continue to be dis covered, and their importance is increasingly recognized in both physical and life sciences. Because the subject matter is so broad and affects so many disciplines, in organizing this Symposium, I sought participation of speakers with the broadest possible range of interests. Twenty-two accepted my invitation. To supplement the verbal presentations, the Proceedings include five closely re lated invited contributions. The ideas expressed are those of the various authors and are not necessarily approved or rejected by any agency of the United States Government. No official recommendation concerning the sub ject matter or products discussed is implied in this book. This book encompasses many aspects of this multifaceted field. Topics covered represent biochemical, immunochemical, bioorganic, biophysical, metabolic, nutritional, medical, physiological, toxi cological, environmental, textile, and analytical interests. The discoveries and developments in any of these areas inevitably illumine others. I feel that a main objective of this Symposium, bringing together scientists with widely varied experiences yet with common interests in protein-metal interactions, so that new understanding and new ideas would result has been realized. I hope that the reader enjoys and benefits from reading about the fascinat ing interactions of metal ions and proteins as much as I did.

Author : G. Weber
Publisher : Springer
Page : 312 pages
File Size : 55,8 Mb
Release : 1992-05-31
Category : Medical
ISBN : UOM:39015025283865

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Protein Interactions by G. Weber Pdf

A study of the thermodynamics of protein-protein and protein-ligand interactions. The author explains the energetics of protein interactions and gives a thorough account of the complicated biophysics that occur when the effects of multiple, complex molecules are taken into account.